Click here to switch to the map view.

The map label for this gene is rimO

Identifier: 116620691

GI number: 116620691

Start: 1926091

End: 1927488

Strand: Reverse

Name: rimO

Synonym: Acid_1571

Alternate gene names: 116620691

Gene position: 1927488-1926091 (Counterclockwise)

Preceding gene: 116620692

Following gene: 116620690

Centisome position: 19.34

GC content: 61.87

Gene sequence:

>1398_bases
TTGAAAGTCGGTTTCATCAGTCTGGGCTGTCCCAAAAACCTCGTCGATAGTGAAGTCATGATGGGTCAACTCGTGGCCAA
GGGCCATGAGCTGACGTCGCATCCTGATCAGGCGGACGTGTTGGTGGTCAACACCTGCAGCTTCATCGACCCCGCGAAAA
AAGAGTCTGTTGACACCATCCTGGAGATGGCCGAGTACAAGAAGATCGGCCGCGCCAAGAAGCTGATCGTCGCGGGCTGC
CTGGTGGAGCGATATCGGGGCGATATCCGCACCGAGATGCCCGAAGTCGATGCGTTGATCGGCACCAACGAGCTGGATAG
CATCGTGGATATCTGCGAGGGCATGCCGCCCTCCACCAACCCGCTGGAACCTTATCTGTATCACGACCTGACGCCGCGCG
TGCTGGCCACGCCGCGCCACTTCGCCTACATGAAGATCGCCGAGGGCTGCGATCATCCGTGCACTTTCTGTGTGATTCCG
CAGTATCGCGGCGCCTTCCGCAGCCGGCGCTTCGAATCCGTGGTTTCCGAGGCCACACGCCTCTTCCAGCAGGGCATCCG
CGAGATCAACCTCATCGGACAGGACACCACCTGCTACGGCGAAGACCTCGGGCTGAAGGACGGACTGGCCGAACTGCTGG
CACGCCTCGCGCAGATCGAGACGCCGCAGGAAAAATGGATTCGCTTCCTGTACGCCTATCCGAACAAGGTCACCCAGAAA
CTGCTCGACACCCTGGCCGAACACGCGGCCCTGGCCAAGTACATCGACATGCCGCTGCAGCATGCCAGCGCCAACGTGCT
CAAGCGCATGAAGCGCGGGGCGTCGGGCGACATCTTCCTCAAACTGATCGAGCGCATCCGCCGTACGATTCCCGGCGTTG
CGATCCGCACGAGTTTCATCGTCGGCTTCCCGGGCGAAACGGCCGCCGATTTCGACGAGCTATGCGCGTTCGTGGAAGCG
GCGAAGTTCGATAACCTCGGCGTGTTCACCTACTCGGACGAAGACACCAGCGCCAGCTACGCGCTGGACGGCAAGGTGGA
TGGCCGCACGATTCAGAACCGGAAGCGCCGCTTGATGGCGATCCAGCGCAAGATCGCGCGCGCCCGCAACCGCGGGCTTG
TCGGCAAGGAAGTTCCGGTACTGGTCAGCGGAGTTTCGGGCGAGACCGACCTGCTGTGGGAAGCGCGGATGTCCACGCAG
GCGCCCGAGATCGATGGCGTAACACTGATTAACGATTTTGAAGGCTCGGAGCCGCGGGCCGGCGAGATCCGCCGCCTGCG
CATCACCGAAGCCCACGATTACGACGTGGTGGGAACGCTGTTAGCGCCCACCGAACCGGCCCCGGTGCTCCCCGCCGGCC
CGGGCCTCATCAACATTGAATCTTTAGTTGCCGTATGA

Upstream 100 bases:

>100_bases
TGCGGCGGCGAACTCTTCGCGGCATGCGAGTACACAAAGGCAGAGTACTACAATGCTATCCTGGTAGAGATCCTTCCGTA
GTTAGGTCTGGTATACACGC

Downstream 100 bases:

>100_bases
CCGTGAAGTGTCCGATTTGTAAAAAGGTGGAAGTCGCGGTGGGCGATCCGGAATTCCCTTTCTGCAGCGAGCGCTGCCGC
ATCATCGATTTGGGCAACTG

Product: MiaB-like tRNA modifying protein YliG

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MKVGFISLGCPKNLVDSEVMMGQLVAKGHELTSHPDQADVLVVNTCSFIDPAKKESVDTILEMAEYKKIGRAKKLIVAGC
LVERYRGDIRTEMPEVDALIGTNELDSIVDICEGMPPSTNPLEPYLYHDLTPRVLATPRHFAYMKIAEGCDHPCTFCVIP
QYRGAFRSRRFESVVSEATRLFQQGIREINLIGQDTTCYGEDLGLKDGLAELLARLAQIETPQEKWIRFLYAYPNKVTQK
LLDTLAEHAALAKYIDMPLQHASANVLKRMKRGASGDIFLKLIERIRRTIPGVAIRTSFIVGFPGETAADFDELCAFVEA
AKFDNLGVFTYSDEDTSASYALDGKVDGRTIQNRKRRLMAIQRKIARARNRGLVGKEVPVLVSGVSGETDLLWEARMSTQ
APEIDGVTLINDFEGSEPRAGEIRRLRITEAHDYDVVGTLLAPTEPAPVLPAGPGLINIESLVAV

Sequences:

>Translated_465_residues
MKVGFISLGCPKNLVDSEVMMGQLVAKGHELTSHPDQADVLVVNTCSFIDPAKKESVDTILEMAEYKKIGRAKKLIVAGC
LVERYRGDIRTEMPEVDALIGTNELDSIVDICEGMPPSTNPLEPYLYHDLTPRVLATPRHFAYMKIAEGCDHPCTFCVIP
QYRGAFRSRRFESVVSEATRLFQQGIREINLIGQDTTCYGEDLGLKDGLAELLARLAQIETPQEKWIRFLYAYPNKVTQK
LLDTLAEHAALAKYIDMPLQHASANVLKRMKRGASGDIFLKLIERIRRTIPGVAIRTSFIVGFPGETAADFDELCAFVEA
AKFDNLGVFTYSDEDTSASYALDGKVDGRTIQNRKRRLMAIQRKIARARNRGLVGKEVPVLVSGVSGETDLLWEARMSTQ
APEIDGVTLINDFEGSEPRAGEIRRLRITEAHDYDVVGTLLAPTEPAPVLPAGPGLINIESLVAV
>Mature_465_residues
MKVGFISLGCPKNLVDSEVMMGQLVAKGHELTSHPDQADVLVVNTCSFIDPAKKESVDTILEMAEYKKIGRAKKLIVAGC
LVERYRGDIRTEMPEVDALIGTNELDSIVDICEGMPPSTNPLEPYLYHDLTPRVLATPRHFAYMKIAEGCDHPCTFCVIP
QYRGAFRSRRFESVVSEATRLFQQGIREINLIGQDTTCYGEDLGLKDGLAELLARLAQIETPQEKWIRFLYAYPNKVTQK
LLDTLAEHAALAKYIDMPLQHASANVLKRMKRGASGDIFLKLIERIRRTIPGVAIRTSFIVGFPGETAADFDELCAFVEA
AKFDNLGVFTYSDEDTSASYALDGKVDGRTIQNRKRRLMAIQRKIARARNRGLVGKEVPVLVSGVSGETDLLWEARMSTQ
APEIDGVTLINDFEGSEPRAGEIRRLRITEAHDYDVVGTLLAPTEPAPVLPAGPGLINIESLVAV

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=425, Percent_Identity=28, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI28872784, Length=425, Percent_Identity=28, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI93277076, Length=343, Percent_Identity=28.5714285714286, Blast_Score=145, Evalue=6e-35,
Organism=Escherichia coli, GI1787057, Length=455, Percent_Identity=37.8021978021978, Blast_Score=281, Evalue=4e-77,
Organism=Escherichia coli, GI1786882, Length=450, Percent_Identity=26.8888888888889, Blast_Score=159, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17553146, Length=474, Percent_Identity=25.3164556962025, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI71996771, Length=302, Percent_Identity=26.8211920529801, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21356207, Length=429, Percent_Identity=27.2727272727273, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI19922432, Length=405, Percent_Identity=27.9012345679012, Blast_Score=137, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_SOLUE (Q028J0)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_822847.1
- STRING:   Q028J0
- GeneID:   4430234
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_1571
- NMPDR:   fig|234267.9.peg.1501
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- PhylomeDB:   Q028J0
- ProtClustDB:   CLSK774702
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 51438; Mature: 51438

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVGFISLGCPKNLVDSEVMMGQLVAKGHELTSHPDQADVLVVNTCSFIDPAKKESVDTI
CCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHH
LEMAEYKKIGRAKKLIVAGCLVERYRGDIRTEMPEVDALIGTNELDSIVDICEGMPPSTN
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCHHHHHHCCCHHHHHHHHHCCCCCCCC
PLEPYLYHDLTPRVLATPRHFAYMKIAEGCDHPCTFCVIPQYRGAFRSRRFESVVSEATR
CCCCHHHCCCCCHHHCCCCHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHH
LFQQGIREINLIGQDTTCYGEDLGLKDGLAELLARLAQIETPQEKWIRFLYAYPNKVTQK
HHHHHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHH
LLDTLAEHAALAKYIDMPLQHASANVLKRMKRGASGDIFLKLIERIRRTIPGVAIRTSFI
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEEEE
VGFPGETAADFDELCAFVEAAKFDNLGVFTYSDEDTSASYALDGKVDGRTIQNRKRRLMA
EECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHHH
IQRKIARARNRGLVGKEVPVLVSGVSGETDLLWEARMSTQAPEIDGVTLINDFEGSEPRA
HHHHHHHHHHCCCCCCCCCEEEECCCCCCHHEEHHHHCCCCCCCCCEEEEECCCCCCCCC
GEIRRLRITEAHDYDVVGTLLAPTEPAPVLPAGPGLINIESLVAV
CCEEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCEEEHHHHHCC
>Mature Secondary Structure
MKVGFISLGCPKNLVDSEVMMGQLVAKGHELTSHPDQADVLVVNTCSFIDPAKKESVDTI
CCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHH
LEMAEYKKIGRAKKLIVAGCLVERYRGDIRTEMPEVDALIGTNELDSIVDICEGMPPSTN
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCHHHHHHCCCHHHHHHHHHCCCCCCCC
PLEPYLYHDLTPRVLATPRHFAYMKIAEGCDHPCTFCVIPQYRGAFRSRRFESVVSEATR
CCCCHHHCCCCCHHHCCCCHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHH
LFQQGIREINLIGQDTTCYGEDLGLKDGLAELLARLAQIETPQEKWIRFLYAYPNKVTQK
HHHHHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHH
LLDTLAEHAALAKYIDMPLQHASANVLKRMKRGASGDIFLKLIERIRRTIPGVAIRTSFI
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEEEE
VGFPGETAADFDELCAFVEAAKFDNLGVFTYSDEDTSASYALDGKVDGRTIQNRKRRLMA
EECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHHH
IQRKIARARNRGLVGKEVPVLVSGVSGETDLLWEARMSTQAPEIDGVTLINDFEGSEPRA
HHHHHHHHHHCCCCCCCCCEEEECCCCCCHHEEHHHHCCCCCCCCCEEEEECCCCCCCCC
GEIRRLRITEAHDYDVVGTLLAPTEPAPVLPAGPGLINIESLVAV
CCEEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA