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The map label for this gene is mdh [H]

Identifier: 116620669

GI number: 116620669

Start: 1900924

End: 1901856

Strand: Reverse

Name: mdh [H]

Synonym: Acid_1549

Alternate gene names: 116620669

Gene position: 1901856-1900924 (Counterclockwise)

Preceding gene: 116620671

Following gene: 116620656

Centisome position: 19.08

GC content: 60.02

Gene sequence:

>933_bases
TTGCGAAAGAAAGTCACAGTTGTGGGCGCCGGAAACGTCGGCGCGAACTGCGCGGTGCGCATTGCCGATAAGGAACTCGC
CGATGTCGTCCTCGTGGACGTGGTGGAGGGTGTTCCTCAGGGTAAGGGGCTCGATCTGCTTCAGTCCGGTCCCGTACAGG
GCTACGATGTGAACATCACGGGCGCGAACGACTACGAGCCCACCGCGAATTCCGATATCGCGATCATTACGGCGGGCTTC
CCGCGCAAACCGGGAATGAGCCGCGACGATTTGCTGCTCGCCAACTTCGAGGTCATTAAGACGGCGACCGAGCAGGTGGC
GAAGTATTCACCGAACTGCATCATCATCGTGGTAACGAATCCGCTGGACGCCATGGCGCAGGCGGCGTTCCACGTTTCCA
AGTTCAGCAAGAACCGCGTGATCGGCATGGCGGGCGTGCTGGATAGCGCCCGATTCCGCACTTTCATCGCGCAGGAACTG
AATGTCTCCGTGGAGAATGTGGTCGGCGTGGTCATGGGCGGACATGGCGACACCATGGTGCCGCTGGTGCGGCTCTGCAA
CGTCTCCGGCATCCCGCTCACCGAACTGATGGATCAGGCCACCATCGATAAGATCGTGGATCGCACGGCCAACGGCGGCG
CAGAGATCGTCAAGCACCTCAAGACGGGCAGCGCCTACTATGCCCCTTCGGCGGCCGCGGTCGAAATGGCCGAGTCGATC
ATTAAGGATAAGAAGAAGGTCCTGCCGTGCGCCGCGTACCTCGAAGGCGAGTACGGCATCAACGGTCTCTACGTCGGCGT
GCCGGTGAAACTCGGCGCGCGCGGCATCGAGAAGATTTACGAGATCAAGTTGACGGCGGACGAACAGGCGAAACTCAAGA
AGAGCGCCGATTCCGTACAGGAACTGGTGAACGTTCTCAAGCCCAAGATGTAA

Upstream 100 bases:

>100_bases
GGCACGACTTGTGTCACATCGCGAGCCGTGTGCCCGGCCCTTTGGCGCGCATGACGATATAATCAGGTTCGGTATTCCCC
AAAAACATAAGGAGTCAGTC

Downstream 100 bases:

>100_bases
TCGGGGAGCGGAATCGCCGATTGGGCCACGGAGCGCGGCGCCTGTGGCCCAATCACAGCGAAGGCAGCAGCGCGGTATTC
AACCAGAAGCGTTCGATGGA

Product: malate dehydrogenase (NAD)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MRKKVTVVGAGNVGANCAVRIADKELADVVLVDVVEGVPQGKGLDLLQSGPVQGYDVNITGANDYEPTANSDIAIITAGF
PRKPGMSRDDLLLANFEVIKTATEQVAKYSPNCIIIVVTNPLDAMAQAAFHVSKFSKNRVIGMAGVLDSARFRTFIAQEL
NVSVENVVGVVMGGHGDTMVPLVRLCNVSGIPLTELMDQATIDKIVDRTANGGAEIVKHLKTGSAYYAPSAAAVEMAESI
IKDKKKVLPCAAYLEGEYGINGLYVGVPVKLGARGIEKIYEIKLTADEQAKLKKSADSVQELVNVLKPKM

Sequences:

>Translated_310_residues
MRKKVTVVGAGNVGANCAVRIADKELADVVLVDVVEGVPQGKGLDLLQSGPVQGYDVNITGANDYEPTANSDIAIITAGF
PRKPGMSRDDLLLANFEVIKTATEQVAKYSPNCIIIVVTNPLDAMAQAAFHVSKFSKNRVIGMAGVLDSARFRTFIAQEL
NVSVENVVGVVMGGHGDTMVPLVRLCNVSGIPLTELMDQATIDKIVDRTANGGAEIVKHLKTGSAYYAPSAAAVEMAESI
IKDKKKVLPCAAYLEGEYGINGLYVGVPVKLGARGIEKIYEIKLTADEQAKLKKSADSVQELVNVLKPKM
>Mature_310_residues
MRKKVTVVGAGNVGANCAVRIADKELADVVLVDVVEGVPQGKGLDLLQSGPVQGYDVNITGANDYEPTANSDIAIITAGF
PRKPGMSRDDLLLANFEVIKTATEQVAKYSPNCIIIVVTNPLDAMAQAAFHVSKFSKNRVIGMAGVLDSARFRTFIAQEL
NVSVENVVGVVMGGHGDTMVPLVRLCNVSGIPLTELMDQATIDKIVDRTANGGAEIVKHLKTGSAYYAPSAAAVEMAESI
IKDKKKVLPCAAYLEGEYGINGLYVGVPVKLGARGIEKIYEIKLTADEQAKLKKSADSVQELVNVLKPKM

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=315, Percent_Identity=39.0476190476191, Blast_Score=215, Evalue=4e-56,
Organism=Homo sapiens, GI221136809, Length=315, Percent_Identity=39.0476190476191, Blast_Score=215, Evalue=4e-56,
Organism=Homo sapiens, GI15082234, Length=318, Percent_Identity=35.8490566037736, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI5031857, Length=308, Percent_Identity=38.6363636363636, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI260099723, Length=308, Percent_Identity=38.6363636363636, Blast_Score=199, Evalue=4e-51,
Organism=Homo sapiens, GI291575128, Length=313, Percent_Identity=37.6996805111821, Blast_Score=191, Evalue=8e-49,
Organism=Homo sapiens, GI4557032, Length=313, Percent_Identity=37.6996805111821, Blast_Score=191, Evalue=8e-49,
Organism=Homo sapiens, GI9257228, Length=308, Percent_Identity=35.3896103896104, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI4504973, Length=308, Percent_Identity=35.3896103896104, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI260099727, Length=222, Percent_Identity=40.0900900900901, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI260099725, Length=204, Percent_Identity=42.6470588235294, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI207028494, Length=308, Percent_Identity=30.8441558441558, Blast_Score=127, Evalue=9e-30,
Organism=Homo sapiens, GI103472011, Length=322, Percent_Identity=26.0869565217391, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI21735621, Length=253, Percent_Identity=30.0395256916996, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI103472015, Length=179, Percent_Identity=28.4916201117318, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1789632, Length=241, Percent_Identity=33.6099585062241, Blast_Score=97, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17535107, Length=298, Percent_Identity=31.5436241610738, Blast_Score=145, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17554310, Length=235, Percent_Identity=32.3404255319149, Blast_Score=100, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6322765, Length=223, Percent_Identity=30.0448430493274, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320125, Length=211, Percent_Identity=29.8578199052133, Blast_Score=83, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6324446, Length=170, Percent_Identity=30, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI17136226, Length=303, Percent_Identity=33.003300330033, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI45550422, Length=309, Percent_Identity=28.1553398058252, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24647881, Length=305, Percent_Identity=30.1639344262295, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24663599, Length=231, Percent_Identity=29.4372294372294, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24663595, Length=258, Percent_Identity=27.906976744186, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 32957; Mature: 32957

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKKVTVVGAGNVGANCAVRIADKELADVVLVDVVEGVPQGKGLDLLQSGPVQGYDVNIT
CCCEEEEEECCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCEEEEEE
GANDYEPTANSDIAIITAGFPRKPGMSRDDLLLANFEVIKTATEQVAKYSPNCIIIVVTN
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEEECC
PLDAMAQAAFHVSKFSKNRVIGMAGVLDSARFRTFIAQELNVSVENVVGVVMGGHGDTMV
CHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCCHHH
PLVRLCNVSGIPLTELMDQATIDKIVDRTANGGAEIVKHLKTGSAYYAPSAAAVEMAESI
HHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEECCCHHHHHHHHHH
IKDKKKVLPCAAYLEGEYGINGLYVGVPVKLGARGIEKIYEIKLTADEQAKLKKSADSVQ
HHHHHHHCCHHHEECCCCCCCEEEEECCHHHCHHHHHEEEEEEECCCHHHHHHHHHHHHH
ELVNVLKPKM
HHHHHHCCCC
>Mature Secondary Structure
MRKKVTVVGAGNVGANCAVRIADKELADVVLVDVVEGVPQGKGLDLLQSGPVQGYDVNIT
CCCEEEEEECCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCEEEEEE
GANDYEPTANSDIAIITAGFPRKPGMSRDDLLLANFEVIKTATEQVAKYSPNCIIIVVTN
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEEECC
PLDAMAQAAFHVSKFSKNRVIGMAGVLDSARFRTFIAQELNVSVENVVGVVMGGHGDTMV
CHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCCHHH
PLVRLCNVSGIPLTELMDQATIDKIVDRTANGGAEIVKHLKTGSAYYAPSAAAVEMAESI
HHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEECCCHHHHHHHHHH
IKDKKKVLPCAAYLEGEYGINGLYVGVPVKLGARGIEKIYEIKLTADEQAKLKKSADSVQ
HHHHHHHCCHHHEECCCCCCCEEEEECCHHHCHHHHHEEEEEEECCCHHHHHHHHHHHHH
ELVNVLKPKM
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA