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The map label for this gene is ycsA [H]

Identifier: 116620650

GI number: 116620650

Start: 1875935

End: 1876999

Strand: Direct

Name: ycsA [H]

Synonym: Acid_1530

Alternate gene names: 116620650

Gene position: 1875935-1876999 (Clockwise)

Preceding gene: 116620648

Following gene: 116620651

Centisome position: 18.82

GC content: 62.82

Gene sequence:

>1065_bases
ATGAAGACTTTTACGATTGGCGTGATCGCCGGCGACGGGGTGGGCACCGAGGTCACCCCGCAGGCAAAACGCGTTCTGGA
AAAGAGCGCCCACAAGCATCAGGTCACCTTTTCCTTCCAGGATTTCGAATGGGGCGCGGAGCACTTTTTCCGTTGGGGCC
GCATGATGCCCGCTGGCGCCATCGATCTCCTCCAGCCTTGCGACGCGATCCTGCTCGGCGCCGTGGGCCATCCCGAAATT
CCCGACCACACGACGCTCAACGGTCTCCTCCTGCCCATCCGCCGCGCCTTCGACCAGTACGCCAACGTGCGGCCGGCTTA
TCTCTATCCGGGTGTGCAGTCTCCACTCGCCCGGCCAAAGGGCGGCGATATCGATTTCGTGGTGGTCCGCGAGAACACTG
AGGGTGAGTACGCTCAGGTGGGCGGCTTCGTCTACCACCTCCAGCCCGAAGAGATCGCGATCCAGACCTCGGTATTCACC
CGCCGCGGCATCGACCGCATCGTCCGCTTCGCCTTCGAACTTGCCGTGAAGCGCAATAAGAAGCGGCGCGTCACCTCGAT
CACGAAGTCCAACGCGCAGGGCTACAGCATGGTGCTGTGGGACCGCGCCTTCCAGGAAATCGCCGCCCAGTTCCCAGAGG
TCGAGACCGAATCGCTGCTCGTGGACGCCGCGGCGATGAACTTCATCCGCCGCCCTGAGAGCTTCGACGTAGTCGTCGGG
TCGAATCTGTTCGCCGATATTCTGAGCGATATCTCGGCCATCATTGTCGGCAGCATGGGGTTGGCTGCGAGCGCCAACCT
CGATCCGCTGCGCCGCTTTCCCTCGATGTTCGAGCCGGTACACGGCTCCGCGCCCGATATCGCCGGCAAGGGGATCGTCA
ACCCGCTCGCCACGATCCTCAGCGCCGGCATGATGCTTGACCATTTGGGAATGACGGAGGCGGCGAAAGAGCTCGACGCC
GCCGTCGCCGCCATACTCGCCGACGGCAAAGTGAGTACTCCGGACATCGGCGGTAAGAGCAGTACCGAGGAAGTGACCGA
CGCTGTTCTGGAGAAACTCGGCTAG

Upstream 100 bases:

>100_bases
TTTCCTCTGGCAATTGAAGTGAAAGAACCCGTTCTCTGAGCGCGCTCATCTTGCACATTTTCGCAAAACCACCCCCTCCC
GGTACAATTGTCTTCGAGAC

Downstream 100 bases:

>100_bases
CGTGGCCAGGGAAAACACAGGCCGCCACGCGTTCATGGTCGCCGCTGGGATCCTGATCAGCCGCATCGTCGGGCTGGCCA
GGCAGCGCGTCTTTTCGCAT

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: TDH; D-malate dehydrogenase [decarboxylating] [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MKTFTIGVIAGDGVGTEVTPQAKRVLEKSAHKHQVTFSFQDFEWGAEHFFRWGRMMPAGAIDLLQPCDAILLGAVGHPEI
PDHTTLNGLLLPIRRAFDQYANVRPAYLYPGVQSPLARPKGGDIDFVVVRENTEGEYAQVGGFVYHLQPEEIAIQTSVFT
RRGIDRIVRFAFELAVKRNKKRRVTSITKSNAQGYSMVLWDRAFQEIAAQFPEVETESLLVDAAAMNFIRRPESFDVVVG
SNLFADILSDISAIIVGSMGLAASANLDPLRRFPSMFEPVHGSAPDIAGKGIVNPLATILSAGMMLDHLGMTEAAKELDA
AVAAILADGKVSTPDIGGKSSTEEVTDAVLEKLG

Sequences:

>Translated_354_residues
MKTFTIGVIAGDGVGTEVTPQAKRVLEKSAHKHQVTFSFQDFEWGAEHFFRWGRMMPAGAIDLLQPCDAILLGAVGHPEI
PDHTTLNGLLLPIRRAFDQYANVRPAYLYPGVQSPLARPKGGDIDFVVVRENTEGEYAQVGGFVYHLQPEEIAIQTSVFT
RRGIDRIVRFAFELAVKRNKKRRVTSITKSNAQGYSMVLWDRAFQEIAAQFPEVETESLLVDAAAMNFIRRPESFDVVVG
SNLFADILSDISAIIVGSMGLAASANLDPLRRFPSMFEPVHGSAPDIAGKGIVNPLATILSAGMMLDHLGMTEAAKELDA
AVAAILADGKVSTPDIGGKSSTEEVTDAVLEKLG
>Mature_354_residues
MKTFTIGVIAGDGVGTEVTPQAKRVLEKSAHKHQVTFSFQDFEWGAEHFFRWGRMMPAGAIDLLQPCDAILLGAVGHPEI
PDHTTLNGLLLPIRRAFDQYANVRPAYLYPGVQSPLARPKGGDIDFVVVRENTEGEYAQVGGFVYHLQPEEIAIQTSVFT
RRGIDRIVRFAFELAVKRNKKRRVTSITKSNAQGYSMVLWDRAFQEIAAQFPEVETESLLVDAAAMNFIRRPESFDVVVG
SNLFADILSDISAIIVGSMGLAASANLDPLRRFPSMFEPVHGSAPDIAGKGIVNPLATILSAGMMLDHLGMTEAAKELDA
AVAAILADGKVSTPDIGGKSSTEEVTDAVLEKLG

Specific function: Unknown

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=349, Percent_Identity=32.378223495702, Blast_Score=188, Evalue=7e-48,
Organism=Homo sapiens, GI4758582, Length=361, Percent_Identity=31.5789473684211, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI28178838, Length=343, Percent_Identity=30.6122448979592, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI28178816, Length=360, Percent_Identity=26.6666666666667, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI28178821, Length=360, Percent_Identity=26.6666666666667, Blast_Score=121, Evalue=9e-28,
Organism=Homo sapiens, GI28178819, Length=200, Percent_Identity=29.5, Blast_Score=92, Evalue=9e-19,
Organism=Escherichia coli, GI1788101, Length=358, Percent_Identity=49.4413407821229, Blast_Score=337, Evalue=7e-94,
Organism=Escherichia coli, GI87081683, Length=367, Percent_Identity=37.3297002724796, Blast_Score=207, Evalue=7e-55,
Organism=Escherichia coli, GI1787381, Length=367, Percent_Identity=29.1553133514986, Blast_Score=124, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71986051, Length=353, Percent_Identity=32.0113314447592, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17550882, Length=357, Percent_Identity=31.6526610644258, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI25144293, Length=353, Percent_Identity=30.8781869688385, Blast_Score=140, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI17505779, Length=272, Percent_Identity=32.3529411764706, Blast_Score=138, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6322097, Length=364, Percent_Identity=38.7362637362637, Blast_Score=226, Evalue=3e-60,
Organism=Saccharomyces cerevisiae, GI6319830, Length=368, Percent_Identity=35.3260869565217, Blast_Score=172, Evalue=9e-44,
Organism=Saccharomyces cerevisiae, GI6324709, Length=354, Percent_Identity=32.4858757062147, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6324291, Length=355, Percent_Identity=33.8028169014084, Blast_Score=170, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24643268, Length=358, Percent_Identity=32.6815642458101, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24643270, Length=358, Percent_Identity=32.6815642458101, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24661184, Length=354, Percent_Identity=31.0734463276836, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI161078635, Length=266, Percent_Identity=33.8345864661654, Blast_Score=144, Evalue=7e-35,
Organism=Drosophila melanogaster, GI161078633, Length=266, Percent_Identity=33.8345864661654, Blast_Score=144, Evalue=7e-35,
Organism=Drosophila melanogaster, GI24650122, Length=266, Percent_Identity=33.8345864661654, Blast_Score=144, Evalue=7e-35,
Organism=Drosophila melanogaster, GI161078637, Length=266, Percent_Identity=33.8345864661654, Blast_Score=144, Evalue=7e-35,
Organism=Drosophila melanogaster, GI161078639, Length=268, Percent_Identity=33.5820895522388, Blast_Score=144, Evalue=9e-35,
Organism=Drosophila melanogaster, GI281362242, Length=355, Percent_Identity=27.3239436619718, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24648872, Length=355, Percent_Identity=27.3239436619718, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI20130355, Length=266, Percent_Identity=30.0751879699248, Blast_Score=118, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011829 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.93; =4.1.1.73; =1.1.1.83 [H]

Molecular weight: Translated: 38424; Mature: 38424

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTFTIGVIAGDGVGTEVTPQAKRVLEKSAHKHQVTFSFQDFEWGAEHFFRWGRMMPAGA
CCEEEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCH
IDLLQPCDAILLGAVGHPEIPDHTTLNGLLLPIRRAFDQYANVRPAYLYPGVQSPLARPK
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCC
GGDIDFVVVRENTEGEYAQVGGFVYHLQPEEIAIQTSVFTRRGIDRIVRFAFELAVKRNK
CCCEEEEEEEECCCCCHHHHCCEEEEECCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHCC
KRRVTSITKSNAQGYSMVLWDRAFQEIAAQFPEVETESLLVDAAAMNFIRRPESFDVVVG
HHHHHHHHHCCCCCCEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEC
SNLFADILSDISAIIVGSMGLAASANLDPLRRFPSMFEPVHGSAPDIAGKGIVNPLATIL
CHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
SAGMMLDHLGMTEAAKELDAAVAAILADGKVSTPDIGGKSSTEEVTDAVLEKLG
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKTFTIGVIAGDGVGTEVTPQAKRVLEKSAHKHQVTFSFQDFEWGAEHFFRWGRMMPAGA
CCEEEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCH
IDLLQPCDAILLGAVGHPEIPDHTTLNGLLLPIRRAFDQYANVRPAYLYPGVQSPLARPK
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCC
GGDIDFVVVRENTEGEYAQVGGFVYHLQPEEIAIQTSVFTRRGIDRIVRFAFELAVKRNK
CCCEEEEEEEECCCCCHHHHCCEEEEECCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHCC
KRRVTSITKSNAQGYSMVLWDRAFQEIAAQFPEVETESLLVDAAAMNFIRRPESFDVVVG
HHHHHHHHHCCCCCCEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEC
SNLFADILSDISAIIVGSMGLAASANLDPLRRFPSMFEPVHGSAPDIAGKGIVNPLATIL
CHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
SAGMMLDHLGMTEAAKELDAAVAAILADGKVSTPDIGGKSSTEEVTDAVLEKLG
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]