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The map label for this gene is iolD1 [H]

Identifier: 116620604

GI number: 116620604

Start: 1819335

End: 1821197

Strand: Direct

Name: iolD1 [H]

Synonym: Acid_1483

Alternate gene names: 116620604

Gene position: 1819335-1821197 (Clockwise)

Preceding gene: 116620603

Following gene: 116620605

Centisome position: 18.26

GC content: 63.93

Gene sequence:

>1863_bases
TTGGAGACGCGTCTCGTAACGGTGGCTCAGGGACTGGTGAGTTTTCTCAAGAACCAGTACGTGGAGCGTGATGGAAAAGA
GCACCGGTTCTTCGCCGGCATGTTGGGGATCTTCGGGCACGGCAACATCGCCGGCATCGGGCAGGCCCTGCAGCAGAATC
CCGACTTTCCGTACATTCCGGTGCGCAATGAGCAATCTGGCGTGCACCTGGCGGCGGCATACGCCAAGGCGAGCAACCGG
CTGCGCACGTTCGCATGCACATCGTCGATTGGGCCGGGCGCGACAAATATGATCACGGGGGCGGCGCTCGCGACGATCAA
CCGGCTGCCGGTGCTGCTGCTGCCGGGCGATATTTTCGCGCGGCGCAATGTGGCTCCAGTGCTCCAGCAACTGGAATCCT
TCGCGACGCAAGATACGGGCGTCAACGATTGCTTCAAGCCGGTGTCGCGCTACTGGGACCGGATCTACCGTCCGGAGCAA
CTGCTCACGGCCCTGCCCGAAGTGATGCGCGTGTTGACTTCGCCGAGCGATTGCGGGGCGGTCACGCTGGCACTGCCGCA
GGACGTGCAGGCCGAGGCATACGAATACCCGGAGGAGTTCTTTCGCAAACGGGTGTGGCTGATCCGTCGCGGACAGCCCG
ATGAGGTCTCTTTCCAGCGCGCGGTGGAGGCGATCCGCGGGGCGCGCAAGCCGTTGATTGTGGCGGGCGGCGGAGTGCTG
ATGAGCGAGGCATCGGAGGCGCTGGCGCGGTTTGCGTCGCAGACCGGAATCGCCGTGGCAGAGACGCAGGCGGGCAAGGG
ATCGCTGGCGTACGATCATGCGCAGTGCATGGGCGCAATCGGCGCGACGGGCACGCTGGCCGCCAACCGGCTGGCGCATG
ATGCGGATCTGGTGATTGGAATCGGAACGCGCTACTCGGATTTCACGTCGGCATCGATGACGGCGTTCCAGAATCCGGCG
GTGCGTTTCGTCAACATCAACACGGCCGAGTTCGATGCGTATAAGGTATCAGCGATCCCGGTGGTGGCGGATGCGCGGGT
GACGCTCGAGCAACTGGCGACGGCGCTAGCGGACTACCGTGTGCCGGCGGAGTACGCGGCCGAAGCCTGCGGGCTGAAGG
CGAAGTGGGAAGCGGAAACCGAGCGCCTGTTCCACCTGAACAATGCGGGGAAGCCGGCGCAAAGCGAAGTGATCGGCGCG
ATGTGGGAGGCTTCGGGACCGCGCGACGTGCTGCTTTCGGCGGCGGGCAGCCATCCCGGCGATCTGCATAAGCTGTGGCG
CACGCGCACTCCCAACGGGTACCACATGGAGTACGGTTACTCCTGCATGTCGTACGAGATTCCCGGGGCGATGGGGGCGA
AACTGGCCGACCCGACGCGGGAAGTTTACGTTTTCCTGGGCGATGGCACGTATCTGATGATGCCGACGGAGATCGTGACG
TCGGTGCAGGAGGGCATCAAGATCATCATCGTGCTGGTGGATAACCACGGCTTCGCGAGCATCGGCGGGCTGAGCCGCTC
GCTGGGGCAGGGCGGATTCGGCACCAGCTACAAGCTGCGCGGAAAGGAATCCGGACAATTGGACGGCGAGACGCTGACGG
TAGATTACGTGGCGAACGCGCGGAGTTTGGGCGCGCACGCTTTGAAGGCGGGCACGCTGGCGGAGTTGAAGCTAGCCCTC
GAAAAAGCCAAGACGCTGGATCGCACGACGGTGATTGTGGTGGAGACCGACCCGGCGGTTGGAGTGCCGGGTTACGAATC
CTGGTGGGATGTGGCGGTGGCCGAGGTCTCGGAGAGCGAGAGCGTGCGGCATGCGCGGGCGCGATATGACGAGGCGCGTA
AGAAGGAACGCTATTACTTGTGA

Upstream 100 bases:

>100_bases
TGGCCGGTGGCTCAGGCACTGGATTCGATGACGGCCAATCGGGGCCGCGATCTGGACCGGCTGTCGCGCTGGTTTTGAAA
CGAAATCAGGGAGGTGGGGA

Downstream 100 bases:

>100_bases
CGGAAAGCCCCTGGCAGGCCGGCAATGCGCCTGTAGCGCGGCGGCCTGCCCGAATCAACCGCGAAGTCCGGTCAAACGAC
TTTCCGAGAGGAAGCACATG

Product: thiamine pyrophosphate binding domain-containing protein

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase 1 [H]

Number of amino acids: Translated: 620; Mature: 620

Protein sequence:

>620_residues
METRLVTVAQGLVSFLKNQYVERDGKEHRFFAGMLGIFGHGNIAGIGQALQQNPDFPYIPVRNEQSGVHLAAAYAKASNR
LRTFACTSSIGPGATNMITGAALATINRLPVLLLPGDIFARRNVAPVLQQLESFATQDTGVNDCFKPVSRYWDRIYRPEQ
LLTALPEVMRVLTSPSDCGAVTLALPQDVQAEAYEYPEEFFRKRVWLIRRGQPDEVSFQRAVEAIRGARKPLIVAGGGVL
MSEASEALARFASQTGIAVAETQAGKGSLAYDHAQCMGAIGATGTLAANRLAHDADLVIGIGTRYSDFTSASMTAFQNPA
VRFVNINTAEFDAYKVSAIPVVADARVTLEQLATALADYRVPAEYAAEACGLKAKWEAETERLFHLNNAGKPAQSEVIGA
MWEASGPRDVLLSAAGSHPGDLHKLWRTRTPNGYHMEYGYSCMSYEIPGAMGAKLADPTREVYVFLGDGTYLMMPTEIVT
SVQEGIKIIIVLVDNHGFASIGGLSRSLGQGGFGTSYKLRGKESGQLDGETLTVDYVANARSLGAHALKAGTLAELKLAL
EKAKTLDRTTVIVVETDPAVGVPGYESWWDVAVAEVSESESVRHARARYDEARKKERYYL

Sequences:

>Translated_620_residues
METRLVTVAQGLVSFLKNQYVERDGKEHRFFAGMLGIFGHGNIAGIGQALQQNPDFPYIPVRNEQSGVHLAAAYAKASNR
LRTFACTSSIGPGATNMITGAALATINRLPVLLLPGDIFARRNVAPVLQQLESFATQDTGVNDCFKPVSRYWDRIYRPEQ
LLTALPEVMRVLTSPSDCGAVTLALPQDVQAEAYEYPEEFFRKRVWLIRRGQPDEVSFQRAVEAIRGARKPLIVAGGGVL
MSEASEALARFASQTGIAVAETQAGKGSLAYDHAQCMGAIGATGTLAANRLAHDADLVIGIGTRYSDFTSASMTAFQNPA
VRFVNINTAEFDAYKVSAIPVVADARVTLEQLATALADYRVPAEYAAEACGLKAKWEAETERLFHLNNAGKPAQSEVIGA
MWEASGPRDVLLSAAGSHPGDLHKLWRTRTPNGYHMEYGYSCMSYEIPGAMGAKLADPTREVYVFLGDGTYLMMPTEIVT
SVQEGIKIIIVLVDNHGFASIGGLSRSLGQGGFGTSYKLRGKESGQLDGETLTVDYVANARSLGAHALKAGTLAELKLAL
EKAKTLDRTTVIVVETDPAVGVPGYESWWDVAVAEVSESESVRHARARYDEARKKERYYL
>Mature_620_residues
METRLVTVAQGLVSFLKNQYVERDGKEHRFFAGMLGIFGHGNIAGIGQALQQNPDFPYIPVRNEQSGVHLAAAYAKASNR
LRTFACTSSIGPGATNMITGAALATINRLPVLLLPGDIFARRNVAPVLQQLESFATQDTGVNDCFKPVSRYWDRIYRPEQ
LLTALPEVMRVLTSPSDCGAVTLALPQDVQAEAYEYPEEFFRKRVWLIRRGQPDEVSFQRAVEAIRGARKPLIVAGGGVL
MSEASEALARFASQTGIAVAETQAGKGSLAYDHAQCMGAIGATGTLAANRLAHDADLVIGIGTRYSDFTSASMTAFQNPA
VRFVNINTAEFDAYKVSAIPVVADARVTLEQLATALADYRVPAEYAAEACGLKAKWEAETERLFHLNNAGKPAQSEVIGA
MWEASGPRDVLLSAAGSHPGDLHKLWRTRTPNGYHMEYGYSCMSYEIPGAMGAKLADPTREVYVFLGDGTYLMMPTEIVT
SVQEGIKIIIVLVDNHGFASIGGLSRSLGQGGFGTSYKLRGKESGQLDGETLTVDYVANARSLGAHALKAGTLAELKLAL
EKAKTLDRTTVIVVETDPAVGVPGYESWWDVAVAEVSESESVRHARARYDEARKKERYYL

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=517, Percent_Identity=22.4371373307544, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI87081685, Length=501, Percent_Identity=25.3493013972056, Blast_Score=153, Evalue=4e-38,
Organism=Escherichia coli, GI1790104, Length=515, Percent_Identity=23.8834951456311, Blast_Score=127, Evalue=3e-30,
Organism=Escherichia coli, GI1786717, Length=541, Percent_Identity=24.5841035120148, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1787096, Length=520, Percent_Identity=21.1538461538462, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323755, Length=570, Percent_Identity=23.1578947368421, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI19922626, Length=547, Percent_Identity=22.4862888482633, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 67183; Mature: 67183

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METRLVTVAQGLVSFLKNQYVERDGKEHRFFAGMLGIFGHGNIAGIGQALQQNPDFPYIP
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEE
VRNEQSGVHLAAAYAKASNRLRTFACTSSIGPGATNMITGAALATINRLPVLLLPGDIFA
CCCCCCCCEEEHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEECCHHHH
RRNVAPVLQQLESFATQDTGVNDCFKPVSRYWDRIYRPEQLLTALPEVMRVLTSPSDCGA
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCE
VTLALPQDVQAEAYEYPEEFFRKRVWLIRRGQPDEVSFQRAVEAIRGARKPLIVAGGGVL
EEEECCCCCCHHHHCCHHHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCCEEEECCCHH
MSEASEALARFASQTGIAVAETQAGKGSLAYDHAQCMGAIGATGTLAANRLAHDADLVIG
HHHHHHHHHHHHHHCCCEEEECCCCCCCCEEHHHHHHHHCCCCHHHHHHHHCCCCCEEEE
IGTRYSDFTSASMTAFQNPAVRFVNINTAEFDAYKVSAIPVVADARVTLEQLATALADYR
ECCCCCCCCCHHHHHHCCCCEEEEEECCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHCC
VPAEYAAEACGLKAKWEAETERLFHLNNAGKPAQSEVIGAMWEASGPRDVLLSAAGSHPG
CCHHHHHHHCCCCCCCCCCHHHEEEECCCCCCHHHHHHHHHHCCCCCHHHHEECCCCCCH
DLHKLWRTRTPNGYHMEYGYSCMSYEIPGAMGAKLADPTREVYVFLGDGTYLMMPTEIVT
HHHHHHHCCCCCCEEEEECCEEEEEECCCCCCCCCCCCCEEEEEEECCCEEEEECHHHHH
SVQEGIKIIIVLVDNHGFASIGGLSRSLGQGGFGTSYKLRGKESGQLDGETLTVDYVANA
HHHCCEEEEEEEECCCCCEECCCHHHHHCCCCCCCCEEECCCCCCCCCCCEEEEEEECCC
RSLGAHALKAGTLAELKLALEKAKTLDRTTVIVVETDPAVGVPGYESWWDVAVAEVSESE
HHHCHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCHH
SVRHARARYDEARKKERYYL
HHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
METRLVTVAQGLVSFLKNQYVERDGKEHRFFAGMLGIFGHGNIAGIGQALQQNPDFPYIP
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEE
VRNEQSGVHLAAAYAKASNRLRTFACTSSIGPGATNMITGAALATINRLPVLLLPGDIFA
CCCCCCCCEEEHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEECCHHHH
RRNVAPVLQQLESFATQDTGVNDCFKPVSRYWDRIYRPEQLLTALPEVMRVLTSPSDCGA
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCE
VTLALPQDVQAEAYEYPEEFFRKRVWLIRRGQPDEVSFQRAVEAIRGARKPLIVAGGGVL
EEEECCCCCCHHHHCCHHHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCCEEEECCCHH
MSEASEALARFASQTGIAVAETQAGKGSLAYDHAQCMGAIGATGTLAANRLAHDADLVIG
HHHHHHHHHHHHHHCCCEEEECCCCCCCCEEHHHHHHHHCCCCHHHHHHHHCCCCCEEEE
IGTRYSDFTSASMTAFQNPAVRFVNINTAEFDAYKVSAIPVVADARVTLEQLATALADYR
ECCCCCCCCCHHHHHHCCCCEEEEEECCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHCC
VPAEYAAEACGLKAKWEAETERLFHLNNAGKPAQSEVIGAMWEASGPRDVLLSAAGSHPG
CCHHHHHHHCCCCCCCCCCHHHEEEECCCCCCHHHHHHHHHHCCCCCHHHHEECCCCCCH
DLHKLWRTRTPNGYHMEYGYSCMSYEIPGAMGAKLADPTREVYVFLGDGTYLMMPTEIVT
HHHHHHHCCCCCCEEEEECCEEEEEECCCCCCCCCCCCCEEEEEEECCCEEEEECHHHHH
SVQEGIKIIIVLVDNHGFASIGGLSRSLGQGGFGTSYKLRGKESGQLDGETLTVDYVANA
HHHCCEEEEEEEECCCCCEECCCHHHHHCCCCCCCCEEECCCCCCCCCCCEEEEEEECCC
RSLGAHALKAGTLAELKLALEKAKTLDRTTVIVVETDPAVGVPGYESWWDVAVAEVSESE
HHHCHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCHH
SVRHARARYDEARKKERYYL
HHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2 pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; Decarboxylation [C]

Inhibitor: 2-oxobutanoate; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA