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The map label for this gene is polA [H]

Identifier: 116620469

GI number: 116620469

Start: 1656496

End: 1659093

Strand: Direct

Name: polA [H]

Synonym: Acid_1347

Alternate gene names: 116620469

Gene position: 1656496-1659093 (Clockwise)

Preceding gene: 116620468

Following gene: 116620470

Centisome position: 16.62

GC content: 63.66

Gene sequence:

>2598_bases
ATGATGGGCGCAGTGGCCAGACAGAAGCTGTTTCTCATCGATACATTCGGTTTCATTTTCCGCGCGTACCATGCGCGCGC
GCGCACTGGCGCGCCCCCGATGCGGACCAGCACGGGGTTCTCCACCGAGGCGGTATACATCTTCAACAACATGCTGCGCA
AGCTTTCCAAGCAGCATGACCCGCCCTACGTAGCGGCGATTTTCGAGAGCGGCGAGGCTACGCATCGAGTGCAGGAATTC
GCCGAATACAAAGCCAACCGGACAGAGACGCCGCCGGACCTGCTGGATCAGATTCCGTTCGTGCGCCGCGTGCTGGAGGC
GATGCGGATTCCGATTCTCGAATACCCCGGTTTCGAAGCCGACGATGTGATCGGGACGATCACCCGACGCGCGGAGGCGG
AGGGGCTGGACGTGGTGATTGTCTCCAGCGACAAGGACATGCTGCAACTGGTGACGGAGCATGTCTCGATGCTCAACCCC
GCCAAGGACGATAAGCTCTACAACCCCGAAGAAGTCAAGACCTTCATGGGGGTGCCGCCGGTGCAGGTCGCGGACCTGCT
GGCACTGATGGGCGACAGCGTGGACAACATTCCGGGGGCTCCGGGAATCGGCGACAAGGGCGCAAAGCAGATTCTGGAGA
AGTTCGGGAGCATCGAAGCGGCGCTGGAGCGGGCGGCCGAGGTGGAGCGCAAGGTGTATCGCGAGAGCCTGCAGAATAAC
GTGGATCGTATCCGGATGAGCAAGCGCCTGGCCACGATCTCGACCGATGTTCCGATTGAATTCACGCTGGACGCGGTGAA
GGCGCAGGCGCCGGACCCGGGGCTGCTGAAGGCGATCTACAAAGAGATGGAGTTTCACAGCCTGCTCAAGGAGCTGGGGC
CGAGCGAAGACACGCGTGAGCGGCGCTATAGCGTGATTGGAAGCGCAGGCGAATTGCGCGAGTGGCTGGCGGGAGCGGCC
GGGACGCCGGTGGCGGTGGCGATCACGAAATCCACCGAGGGTGAGTTCGCCCTGGATATGATCGCGGCGGATACGATCGG
GCTGGCGCGGAAGCGGGGAGAGGCATGCGCGGTAACGAGCGAACTGATCGTGGAGCTGAAGCCATGGCTGGAGGACGCGG
CCGTGGTGAAGGTGGCCTGCGACGTGAAATCCGCGGTGCTGCAACTGGCGAGGCTCGGGATCGAAGCGCGCGGCTTCGGT
CATGATGTGATGATTTACGGCTTTCTGCTGGATGCCGACCCTGGGGGGTGCGGCCTGGAGAACCAGGCGCACAGACGGCT
GGACCTGAAGTTGGGCGGCGCGGCGGAGCAGCATGCGGATATCACGCTTGAGCTCTACGAACAGCTGGCGCCGGCGATCG
ACGGCCGCGGGTTGCGCAAGCTGTACGACGAGATCGAGCTGCCGCTGACACGCGTGCTGGCGCGGATGGAGCGCACCGGT
ATCCGCATCGATGGGGCGGAGTTGAAGCGACTTTCGGTGTTGATGGAGAGCCAGATTTCGGGGCTCACGGCCGAGATCCA
CGCGCTGGCCGGCAAGCCGTTCAACATCAGCTCTCCGCAACAATTGGGCAAGGTGCTGTTCGAGGACCTGAATTTGCCGG
CGCCGGTGAAGTACGGCAAGGGTAAGACGATTTCCACGGCCGCGGATGTGCTGGACGAACTCGCAGCCGAGCACGAGATC
GTACGCAAGGTGCTGGAGTATCGCCAGCTCACGAAGTTGAAGGGGACGTATGTGGATGCGCTACCGGCGCTGATCGACCC
GGCGACGGGGCGGCTGCACACGAGCTTCAACCAGACAGGAGCGGCGACGGGGCGGCTTTCGAGCTCTAACCCGAACCTGC
AGAACATTCCGATCCGTACTTCCCTGGGGCGGGAGATTCGCGCGGCCTTTGTGCCGAGAGACGGCTGGAAGCTGCTGGTG
GCCGACTACTCGCAGATCGAGCTGCGCCTGCTGGCGCACATGTCGGGCGACGAGCTGCTGGTTCAGGCGTTCCGGGAAGG
CGAGGACATCCACACGCGGACGGCGGCGGAGGTGATGGGGGTGCCGCCGCTGATGGTCACGCCGGAAGCGCGGCGCGATG
CGAAGGCGGTGAACTTCGGCATTGTGTACGGGATCAGCGCGTTTGGACTCGCGGCGCAGCTCGGCATCGGGAGGGCGGAG
GCGGAGCGGTACATCAAGAATTACTTCGCGCGATACGTGGGGGTGAAGCGTTTCATCGAGGAGACGATCGCAGAGGTACG
CAAGACGGGCGTAACGAAGACGCTGTTTGGGCGGGAGCGTCCGATTCCGGAGATCAACGGGCGCAATCCGAACGCGCGTG
GGTTCGCGGAGCGGACGGCGGTCAATTCGCCGCTACAGGGCACGGCCGCGGACTTGATCAAACTGGCGATGGTGCGGATC
GACGCGGCGCTGGCGGCGGGCGGCTTTCAAGCTGCGATGCTGCTCCAGGTGCACGACGAGCTCGTGTTCGAATGCCCGCC
GGAAGAACTCGACGAAGTTTCGCGGATGGTGAAGCGCGAGATGGAGGGCGCCTGCGAGTTGAAGGTGCCGCTGGTAGTGG
ACGTGGGATCGGGCGACAACTGGCGCGACGCGAAATAA

Upstream 100 bases:

>100_bases
GACCGCAGACTGGCGCGCGCGGTCGGCGGTGTGCCGAGCATGAACGAAGGAGCCGCCTGAAACGCGGCTGCAGAAAAAAT
AGTACAAGCCAACTCGGGCT

Downstream 100 bases:

>100_bases
CATCTGTTTTACACTGGCTCTATCCCTGGGGGCTGGAGGACGATGCGAGTCGCGATCGCCGTGGTTTTGTTTTCCGTATG
GCTGGCCGCGCAAACGGTGT

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 865; Mature: 865

Protein sequence:

>865_residues
MMGAVARQKLFLIDTFGFIFRAYHARARTGAPPMRTSTGFSTEAVYIFNNMLRKLSKQHDPPYVAAIFESGEATHRVQEF
AEYKANRTETPPDLLDQIPFVRRVLEAMRIPILEYPGFEADDVIGTITRRAEAEGLDVVIVSSDKDMLQLVTEHVSMLNP
AKDDKLYNPEEVKTFMGVPPVQVADLLALMGDSVDNIPGAPGIGDKGAKQILEKFGSIEAALERAAEVERKVYRESLQNN
VDRIRMSKRLATISTDVPIEFTLDAVKAQAPDPGLLKAIYKEMEFHSLLKELGPSEDTRERRYSVIGSAGELREWLAGAA
GTPVAVAITKSTEGEFALDMIAADTIGLARKRGEACAVTSELIVELKPWLEDAAVVKVACDVKSAVLQLARLGIEARGFG
HDVMIYGFLLDADPGGCGLENQAHRRLDLKLGGAAEQHADITLELYEQLAPAIDGRGLRKLYDEIELPLTRVLARMERTG
IRIDGAELKRLSVLMESQISGLTAEIHALAGKPFNISSPQQLGKVLFEDLNLPAPVKYGKGKTISTAADVLDELAAEHEI
VRKVLEYRQLTKLKGTYVDALPALIDPATGRLHTSFNQTGAATGRLSSSNPNLQNIPIRTSLGREIRAAFVPRDGWKLLV
ADYSQIELRLLAHMSGDELLVQAFREGEDIHTRTAAEVMGVPPLMVTPEARRDAKAVNFGIVYGISAFGLAAQLGIGRAE
AERYIKNYFARYVGVKRFIEETIAEVRKTGVTKTLFGRERPIPEINGRNPNARGFAERTAVNSPLQGTAADLIKLAMVRI
DAALAAGGFQAAMLLQVHDELVFECPPEELDEVSRMVKREMEGACELKVPLVVDVGSGDNWRDAK

Sequences:

>Translated_865_residues
MMGAVARQKLFLIDTFGFIFRAYHARARTGAPPMRTSTGFSTEAVYIFNNMLRKLSKQHDPPYVAAIFESGEATHRVQEF
AEYKANRTETPPDLLDQIPFVRRVLEAMRIPILEYPGFEADDVIGTITRRAEAEGLDVVIVSSDKDMLQLVTEHVSMLNP
AKDDKLYNPEEVKTFMGVPPVQVADLLALMGDSVDNIPGAPGIGDKGAKQILEKFGSIEAALERAAEVERKVYRESLQNN
VDRIRMSKRLATISTDVPIEFTLDAVKAQAPDPGLLKAIYKEMEFHSLLKELGPSEDTRERRYSVIGSAGELREWLAGAA
GTPVAVAITKSTEGEFALDMIAADTIGLARKRGEACAVTSELIVELKPWLEDAAVVKVACDVKSAVLQLARLGIEARGFG
HDVMIYGFLLDADPGGCGLENQAHRRLDLKLGGAAEQHADITLELYEQLAPAIDGRGLRKLYDEIELPLTRVLARMERTG
IRIDGAELKRLSVLMESQISGLTAEIHALAGKPFNISSPQQLGKVLFEDLNLPAPVKYGKGKTISTAADVLDELAAEHEI
VRKVLEYRQLTKLKGTYVDALPALIDPATGRLHTSFNQTGAATGRLSSSNPNLQNIPIRTSLGREIRAAFVPRDGWKLLV
ADYSQIELRLLAHMSGDELLVQAFREGEDIHTRTAAEVMGVPPLMVTPEARRDAKAVNFGIVYGISAFGLAAQLGIGRAE
AERYIKNYFARYVGVKRFIEETIAEVRKTGVTKTLFGRERPIPEINGRNPNARGFAERTAVNSPLQGTAADLIKLAMVRI
DAALAAGGFQAAMLLQVHDELVFECPPEELDEVSRMVKREMEGACELKVPLVVDVGSGDNWRDAK
>Mature_865_residues
MMGAVARQKLFLIDTFGFIFRAYHARARTGAPPMRTSTGFSTEAVYIFNNMLRKLSKQHDPPYVAAIFESGEATHRVQEF
AEYKANRTETPPDLLDQIPFVRRVLEAMRIPILEYPGFEADDVIGTITRRAEAEGLDVVIVSSDKDMLQLVTEHVSMLNP
AKDDKLYNPEEVKTFMGVPPVQVADLLALMGDSVDNIPGAPGIGDKGAKQILEKFGSIEAALERAAEVERKVYRESLQNN
VDRIRMSKRLATISTDVPIEFTLDAVKAQAPDPGLLKAIYKEMEFHSLLKELGPSEDTRERRYSVIGSAGELREWLAGAA
GTPVAVAITKSTEGEFALDMIAADTIGLARKRGEACAVTSELIVELKPWLEDAAVVKVACDVKSAVLQLARLGIEARGFG
HDVMIYGFLLDADPGGCGLENQAHRRLDLKLGGAAEQHADITLELYEQLAPAIDGRGLRKLYDEIELPLTRVLARMERTG
IRIDGAELKRLSVLMESQISGLTAEIHALAGKPFNISSPQQLGKVLFEDLNLPAPVKYGKGKTISTAADVLDELAAEHEI
VRKVLEYRQLTKLKGTYVDALPALIDPATGRLHTSFNQTGAATGRLSSSNPNLQNIPIRTSLGREIRAAFVPRDGWKLLV
ADYSQIELRLLAHMSGDELLVQAFREGEDIHTRTAAEVMGVPPLMVTPEARRDAKAVNFGIVYGISAFGLAAQLGIGRAE
AERYIKNYFARYVGVKRFIEETIAEVRKTGVTKTLFGRERPIPEINGRNPNARGFAERTAVNSPLQGTAADLIKLAMVRI
DAALAAGGFQAAMLLQVHDELVFECPPEELDEVSRMVKREMEGACELKVPLVVDVGSGDNWRDAK

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=444, Percent_Identity=36.036036036036, Blast_Score=265, Evalue=1e-70,
Organism=Homo sapiens, GI139394648, Length=532, Percent_Identity=28.9473684210526, Blast_Score=228, Evalue=2e-59,
Organism=Escherichia coli, GI1790294, Length=938, Percent_Identity=38.6993603411514, Blast_Score=597, Evalue=1e-171,
Organism=Escherichia coli, GI226510970, Length=260, Percent_Identity=28.8461538461538, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI86563272, Length=409, Percent_Identity=32.2738386308068, Blast_Score=184, Evalue=3e-46,
Organism=Drosophila melanogaster, GI17933644, Length=428, Percent_Identity=30.8411214953271, Blast_Score=190, Evalue=4e-48,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR003583
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 94868; Mature: 94868

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMGAVARQKLFLIDTFGFIFRAYHARARTGAPPMRTSTGFSTEAVYIFNNMLRKLSKQHD
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
PPYVAAIFESGEATHRVQEFAEYKANRTETPPDLLDQIPFVRRVLEAMRIPILEYPGFEA
CCEEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCCCCH
DDVIGTITRRAEAEGLDVVIVSSDKDMLQLVTEHVSMLNPAKDDKLYNPEEVKTFMGVPP
HHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCC
VQVADLLALMGDSVDNIPGAPGIGDKGAKQILEKFGSIEAALERAAEVERKVYRESLQNN
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
VDRIRMSKRLATISTDVPIEFTLDAVKAQAPDPGLLKAIYKEMEFHSLLKELGPSEDTRE
HHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHH
RRYSVIGSAGELREWLAGAAGTPVAVAITKSTEGEFALDMIAADTIGLARKRGEACAVTS
HHHHHHCCHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHH
ELIVELKPWLEDAAVVKVACDVKSAVLQLARLGIEARGFGHDVMIYGFLLDADPGGCGLE
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCCC
NQAHRRLDLKLGGAAEQHADITLELYEQLAPAIDGRGLRKLYDEIELPLTRVLARMERTG
CHHHHEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHCC
IRIDGAELKRLSVLMESQISGLTAEIHALAGKPFNISSPQQLGKVLFEDLNLPAPVKYGK
CEECHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
GKTISTAADVLDELAAEHEIVRKVLEYRQLTKLKGTYVDALPALIDPATGRLHTSFNQTG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEECCCCCC
AATGRLSSSNPNLQNIPIRTSLGREIRAAFVPRDGWKLLVADYSQIELRLLAHMSGDELL
CCCCCCCCCCCCCCCCCCHHHCCHHHHHHCCCCCCCEEEEECHHHHHHHHHHHCCCHHHH
VQAFREGEDIHTRTAAEVMGVPPLMVTPEARRDAKAVNFGIVYGISAFGLAAQLGIGRAE
HHHHHCCCHHHHHHHHHHHCCCCEEECCCHHHCCHHEEHHHHHHHHHHHHHHHHCCCHHH
AERYIKNYFARYVGVKRFIEETIAEVRKTGVTKTLFGRERPIPEINGRNPNARGFAERTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
VNSPLQGTAADLIKLAMVRIDAALAAGGFQAAMLLQVHDELVFECPPEELDEVSRMVKRE
CCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
MEGACELKVPLVVDVGSGDNWRDAK
HCCCCEEEEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MMGAVARQKLFLIDTFGFIFRAYHARARTGAPPMRTSTGFSTEAVYIFNNMLRKLSKQHD
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
PPYVAAIFESGEATHRVQEFAEYKANRTETPPDLLDQIPFVRRVLEAMRIPILEYPGFEA
CCEEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCCCCH
DDVIGTITRRAEAEGLDVVIVSSDKDMLQLVTEHVSMLNPAKDDKLYNPEEVKTFMGVPP
HHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCC
VQVADLLALMGDSVDNIPGAPGIGDKGAKQILEKFGSIEAALERAAEVERKVYRESLQNN
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
VDRIRMSKRLATISTDVPIEFTLDAVKAQAPDPGLLKAIYKEMEFHSLLKELGPSEDTRE
HHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHH
RRYSVIGSAGELREWLAGAAGTPVAVAITKSTEGEFALDMIAADTIGLARKRGEACAVTS
HHHHHHCCHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHH
ELIVELKPWLEDAAVVKVACDVKSAVLQLARLGIEARGFGHDVMIYGFLLDADPGGCGLE
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCCC
NQAHRRLDLKLGGAAEQHADITLELYEQLAPAIDGRGLRKLYDEIELPLTRVLARMERTG
CHHHHEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHCC
IRIDGAELKRLSVLMESQISGLTAEIHALAGKPFNISSPQQLGKVLFEDLNLPAPVKYGK
CEECHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
GKTISTAADVLDELAAEHEIVRKVLEYRQLTKLKGTYVDALPALIDPATGRLHTSFNQTG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEECCCCCC
AATGRLSSSNPNLQNIPIRTSLGREIRAAFVPRDGWKLLVADYSQIELRLLAHMSGDELL
CCCCCCCCCCCCCCCCCCHHHCCHHHHHHCCCCCCCEEEEECHHHHHHHHHHHCCCHHHH
VQAFREGEDIHTRTAAEVMGVPPLMVTPEARRDAKAVNFGIVYGISAFGLAAQLGIGRAE
HHHHHCCCHHHHHHHHHHHCCCCEEECCCHHHCCHHEEHHHHHHHHHHHHHHHHCCCHHH
AERYIKNYFARYVGVKRFIEETIAEVRKTGVTKTLFGRERPIPEINGRNPNARGFAERTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
VNSPLQGTAADLIKLAMVRIDAALAAGGFQAAMLLQVHDELVFECPPEELDEVSRMVKRE
CCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
MEGACELKVPLVVDVGSGDNWRDAK
HCCCCEEEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]