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The map label for this gene is rihA [H]

Identifier: 116620464

GI number: 116620464

Start: 1652241

End: 1653281

Strand: Reverse

Name: rihA [H]

Synonym: Acid_1342

Alternate gene names: 116620464

Gene position: 1653281-1652241 (Counterclockwise)

Preceding gene: 116620471

Following gene: 116620463

Centisome position: 16.59

GC content: 61.38

Gene sequence:

>1041_bases
ATGTCTCGTTTTCTGGCGATGTTTCTGGTCGTTACCTGCCTCGCAGCCACGACCCGCGACCTCGTTATCTTCGATACCGA
TTCCGGACTCTTCGGCGACGACGGTGCGGCCCTCGTCATGCTCCTGCGTGGTTCGTCTCAAGAGGTCTTGCAGGGCATTA
CCATTGTTCCCGGCAACGTCTGGGCAGCCCAGGGCGCCGAGTACATGTTCCACATCCTCGACCTGCTCAAGAAGCCGCAA
ATGCAGGTGGCCGTCGGAGCCGAGGCTCCACTGATCCACACCGCCGCCATGTCCAAGGAGTTCGATCGCCGCTGGGGCGG
CCTCGGCTACACCGGCGCCTTTGCCCAGGACCCCACCGCCGTCGTCCCCGCGCCCGGCAGTAAGATCAGTACCCGCAAAC
TCCGCCGCGACGCCATCAACTTCCTCATCTCCGAGATTGAACGCCACCCCGGCGAAATCACCATTCTCGCCCTCGGTCCC
ATGACTAACATTGCGCTCGCCCTCCGCCTTAAGCCCGATATCGAGACCAAAATCAAGCGCATCGTCTTCATGGGAGGCAA
CATTCGCGTCGCCGGGAACGCAACCCCCGCCGCCGAGTTCAATTTCTGGTTCGACCCCGAAGCCGCGCGCATCGTGCTCC
GCTCTCGCATCCCTAAGAAAATGATGTTCGGCCTCGATATCTGCAACCTCGCCAAAATCCGCAAGGCCGAGTTCGACCAG
ATCGCCTCCGCCCGGAATCCCATCGCCGAACTCTTCCGCGAAGATCTCGGCAACCGCTATCCCGGTTTCCTCAAAAAGCC
GGACGCCATCGGCTTCATGTGGGATTCCCTCGCCGCCGCCTACCTGCTCGATCCCGACTTCGTCACCAAAACCGAATCGC
GCTACCTCGACGTGCAAACCACCTGGGGGCAATTCTACGGCTCCACCACACCCCTCGACCGCCGCGCCACCCCCACTGCC
ACCCCGGTCACGGTCATGCTCGACCTCGATTTCAAACGCGTCTTTGCCCTCTACAAAGACCGCCTCGTCAGAAGCGAATA
A

Upstream 100 bases:

>100_bases
CTTTCTTCATGAACCGCATTGTACCCTGCCGCCCCGCCGCGCTTCCCTAACGCCCATACCGTATCCGGGATCCACCTGCT
CCGCGCTATAGTGAGGTTTC

Downstream 100 bases:

>100_bases
CTACCATGCCGGTCGTTGCCGTCAGCCACCCCGCGCAAATCGAACAGGTACGGATCCTCTTTCAGGAGTACTGGGATTCC
TTCGATTTCACACCCTGCTT

Product: inosine/uridine-preferring nucleoside hydrolase

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MSRFLAMFLVVTCLAATTRDLVIFDTDSGLFGDDGAALVMLLRGSSQEVLQGITIVPGNVWAAQGAEYMFHILDLLKKPQ
MQVAVGAEAPLIHTAAMSKEFDRRWGGLGYTGAFAQDPTAVVPAPGSKISTRKLRRDAINFLISEIERHPGEITILALGP
MTNIALALRLKPDIETKIKRIVFMGGNIRVAGNATPAAEFNFWFDPEAARIVLRSRIPKKMMFGLDICNLAKIRKAEFDQ
IASARNPIAELFREDLGNRYPGFLKKPDAIGFMWDSLAAAYLLDPDFVTKTESRYLDVQTTWGQFYGSTTPLDRRATPTA
TPVTVMLDLDFKRVFALYKDRLVRSE

Sequences:

>Translated_346_residues
MSRFLAMFLVVTCLAATTRDLVIFDTDSGLFGDDGAALVMLLRGSSQEVLQGITIVPGNVWAAQGAEYMFHILDLLKKPQ
MQVAVGAEAPLIHTAAMSKEFDRRWGGLGYTGAFAQDPTAVVPAPGSKISTRKLRRDAINFLISEIERHPGEITILALGP
MTNIALALRLKPDIETKIKRIVFMGGNIRVAGNATPAAEFNFWFDPEAARIVLRSRIPKKMMFGLDICNLAKIRKAEFDQ
IASARNPIAELFREDLGNRYPGFLKKPDAIGFMWDSLAAAYLLDPDFVTKTESRYLDVQTTWGQFYGSTTPLDRRATPTA
TPVTVMLDLDFKRVFALYKDRLVRSE
>Mature_345_residues
SRFLAMFLVVTCLAATTRDLVIFDTDSGLFGDDGAALVMLLRGSSQEVLQGITIVPGNVWAAQGAEYMFHILDLLKKPQM
QVAVGAEAPLIHTAAMSKEFDRRWGGLGYTGAFAQDPTAVVPAPGSKISTRKLRRDAINFLISEIERHPGEITILALGPM
TNIALALRLKPDIETKIKRIVFMGGNIRVAGNATPAAEFNFWFDPEAARIVLRSRIPKKMMFGLDICNLAKIRKAEFDQI
ASARNPIAELFREDLGNRYPGFLKKPDAIGFMWDSLAAAYLLDPDFVTKTESRYLDVQTTWGQFYGSTTPLDRRATPTAT
PVTVMLDLDFKRVFALYKDRLVRSE

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=329, Percent_Identity=31.6109422492401, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1788486, Length=315, Percent_Identity=30.7936507936508, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1786213, Length=285, Percent_Identity=30.5263157894737, Blast_Score=89, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI32565474, Length=287, Percent_Identity=30.3135888501742, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320608, Length=105, Percent_Identity=35.2380952380952, Blast_Score=66, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24583915, Length=284, Percent_Identity=30.2816901408451, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24641839, Length=297, Percent_Identity=29.6296296296296, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24641837, Length=215, Percent_Identity=31.1627906976744, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 38412; Mature: 38281

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRFLAMFLVVTCLAATTRDLVIFDTDSGLFGDDGAALVMLLRGSSQEVLQGITIVPGNV
CHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEECCCCHHHHCCCEECCCCC
WAAQGAEYMFHILDLLKKPQMQVAVGAEAPLIHTAAMSKEFDRRWGGLGYTGAFAQDPTA
CHHCCHHHHHHHHHHHCCCCEEEEECCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCE
VVPAPGSKISTRKLRRDAINFLISEIERHPGEITILALGPMTNIALALRLKPDIETKIKR
EECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCHHHHEEE
IVFMGGNIRVAGNATPAAEFNFWFDPEAARIVLRSRIPKKMMFGLDICNLAKIRKAEFDQ
EEEECCCEEEECCCCCCCCEEEEECCHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHH
IASARNPIAELFREDLGNRYPGFLKKPDAIGFMWDSLAAAYLLDPDFVTKTESRYLDVQT
HHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHEECCCHHCCCCCCEEEEEE
TWGQFYGSTTPLDRRATPTATPVTVMLDLDFKRVFALYKDRLVRSE
HHHHHCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SRFLAMFLVVTCLAATTRDLVIFDTDSGLFGDDGAALVMLLRGSSQEVLQGITIVPGNV
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEECCCCHHHHCCCEECCCCC
WAAQGAEYMFHILDLLKKPQMQVAVGAEAPLIHTAAMSKEFDRRWGGLGYTGAFAQDPTA
CHHCCHHHHHHHHHHHCCCCEEEEECCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCE
VVPAPGSKISTRKLRRDAINFLISEIERHPGEITILALGPMTNIALALRLKPDIETKIKR
EECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCHHHHEEE
IVFMGGNIRVAGNATPAAEFNFWFDPEAARIVLRSRIPKKMMFGLDICNLAKIRKAEFDQ
EEEECCCEEEECCCCCCCCEEEEECCHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHH
IASARNPIAELFREDLGNRYPGFLKKPDAIGFMWDSLAAAYLLDPDFVTKTESRYLDVQT
HHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHEECCCHHCCCCCCEEEEEE
TWGQFYGSTTPLDRRATPTATPVTVMLDLDFKRVFALYKDRLVRSE
HHHHHCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA