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The map label for this gene is yfkN [H]

Identifier: 116620417

GI number: 116620417

Start: 1594242

End: 1595816

Strand: Direct

Name: yfkN [H]

Synonym: Acid_1294

Alternate gene names: 116620417

Gene position: 1594242-1595816 (Clockwise)

Preceding gene: 116620415

Following gene: 116620421

Centisome position: 16.0

GC content: 64.44

Gene sequence:

>1575_bases
ATGGATACCATACGCCGCCTGCTTCCGATCCTGCTGTTGTCCGCCGGCCTGCTGCGCCCCGCCGCAGTCACCGTGACGCT
GCTCGCCACCACCGATATGCACGGCAATCTCGTGCCCGTGGACTATGTCACCGGCGCCACCGCTCCCCGGGGACTCGCCC
GCCTGGCCACCCTCATCCGGGCCGTCCGCGCGGAAAACCCGAATACCATCCTGGTCGATTGCGGCGACACCATCCAGGGC
ACGCCGCTCGAAGGCGTCTATCAACGTGCGCTGCGCGCCGGAAATCCGCCGGCCCCTGGCGACCCCATGATGCGCGCCAT
GAACCTGCTGGGCTACGACGCCATGACCGTCGGCAATCACGAATTCAACGCTGGCCTCGCCAATTTCAATAGCGCGCGCC
GCGATGCCCGCTTCCCGTGGATCTCCGCCAACATCGCTGTCGCCACCGGCGGCGCCGAGCGCGCCTTCGCCCCATACATC
GTGAAGACCGTCGCCGGCGTCAAAGTCGCCGTGATCGGCCTGACCACGCCGATGGTGCCCCTGTGGGAGAAGCCCGAAAA
CCTCGGCGCCTACCGCTTCACCCCGCCCGTGGAAGCCGTGAAGTCCGCCATCGCCAAACTGCGCCGCGACGAACGTCCCG
ACCTGATCGTGGTTACCGCACACTCCGGACTCGGCCGCAATCTGCAAACCAACCAGGATGAAGAAACCGTGGAAAACTTC
GTCCACGAACTTGCGCGGCAGATGCCGGAGCTGGACGCGATCGTCTTCGGCCATTCCCACCAGCAACTTGAAGGTGCGCT
CGTCGGCAACGTGCTCCTGGTCCAGCCCAAAAACGCGGCCGCCTCGCTCGCGCGTATCGATTTCACCTTCGATGGCACGC
TGATTTCCAAGCGCAGCCGGCTGATTCCCGCCACCGCCCAGACCGCCGCCGCCCCCGACCTGATGGCGCTCGCCGCACCC
TACGAAGCCGCCACGCAGCGCTACCTCAACACGCCAGTCGCCCATTGCGATCGCGAGCTGACCGCAACTCGCGCGCGTGA
GGAAGACAGCGCAATTGTGGATGCCATCCAGCGTGTGCAGTTGTACTACGCCAAGGCGGATGTCTCCTTCACCGCGCTCT
TCGATCCCCAGGTGCGGATTCCGCGGGGCGAAGTCACCGTCCGGCAGATCGCGGCCCTGTACCCTTACGACAACGAACTG
TTCGGCATCGAGGGTACGGGTAAGATGGTGAAAGACGCGCTCGAAAATGCCGCGCGCTATTTTTCGGCGAAGCCGATCTT
TGGATTTAATTACGACATGGCGGAAGGTGTCGAGTACGAGATCGATCGCTCGCGCCCCGAAGGCGACCGCATTCGCAACC
TTCGCTGGAAAGGCCGCCCGCTGGACCCCGCGCAGCGCCTGCGCATCGCCATCAACAACTACCGCGCCGGCGGCTCCGGC
GGCTACGACATGTTCCGCGGCGCCAAAATCCTCTGGCGCTCCGGCGACGACATCCGCGATCTGCTGATCCGCTACTACAC
CGAGCGCAAATCAATCCCGGCCGAAGCCACCAATAACTGGAAGCTCAAGAATTAA

Upstream 100 bases:

>100_bases
GGGCGTGGTCTGCGCAAACGCGCATGCAGCGATCAGGAAAAGAGTAGACAGCCGTTTCATCTGATTCTCCGTAAAGACTC
ATTCTATTCTATAGACAGCG

Downstream 100 bases:

>100_bases
GAGAGTCACGAATGTCCATTCGTGGCCCAAATTGCTTCACTTCTTAATCCCGAGTTCCGCCTGCAATTGCCGGTACGCCG
CCTCTTCGTAGTTGGTTTTC

Product: 2',3'-cyclic-nucleotide 2'-phosphodiesterase

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MDTIRRLLPILLLSAGLLRPAAVTVTLLATTDMHGNLVPVDYVTGATAPRGLARLATLIRAVRAENPNTILVDCGDTIQG
TPLEGVYQRALRAGNPPAPGDPMMRAMNLLGYDAMTVGNHEFNAGLANFNSARRDARFPWISANIAVATGGAERAFAPYI
VKTVAGVKVAVIGLTTPMVPLWEKPENLGAYRFTPPVEAVKSAIAKLRRDERPDLIVVTAHSGLGRNLQTNQDEETVENF
VHELARQMPELDAIVFGHSHQQLEGALVGNVLLVQPKNAAASLARIDFTFDGTLISKRSRLIPATAQTAAAPDLMALAAP
YEAATQRYLNTPVAHCDRELTATRAREEDSAIVDAIQRVQLYYAKADVSFTALFDPQVRIPRGEVTVRQIAALYPYDNEL
FGIEGTGKMVKDALENAARYFSAKPIFGFNYDMAEGVEYEIDRSRPEGDRIRNLRWKGRPLDPAQRLRIAINNYRAGGSG
GYDMFRGAKILWRSGDDIRDLLIRYYTERKSIPAEATNNWKLKN

Sequences:

>Translated_524_residues
MDTIRRLLPILLLSAGLLRPAAVTVTLLATTDMHGNLVPVDYVTGATAPRGLARLATLIRAVRAENPNTILVDCGDTIQG
TPLEGVYQRALRAGNPPAPGDPMMRAMNLLGYDAMTVGNHEFNAGLANFNSARRDARFPWISANIAVATGGAERAFAPYI
VKTVAGVKVAVIGLTTPMVPLWEKPENLGAYRFTPPVEAVKSAIAKLRRDERPDLIVVTAHSGLGRNLQTNQDEETVENF
VHELARQMPELDAIVFGHSHQQLEGALVGNVLLVQPKNAAASLARIDFTFDGTLISKRSRLIPATAQTAAAPDLMALAAP
YEAATQRYLNTPVAHCDRELTATRAREEDSAIVDAIQRVQLYYAKADVSFTALFDPQVRIPRGEVTVRQIAALYPYDNEL
FGIEGTGKMVKDALENAARYFSAKPIFGFNYDMAEGVEYEIDRSRPEGDRIRNLRWKGRPLDPAQRLRIAINNYRAGGSG
GYDMFRGAKILWRSGDDIRDLLIRYYTERKSIPAEATNNWKLKN
>Mature_524_residues
MDTIRRLLPILLLSAGLLRPAAVTVTLLATTDMHGNLVPVDYVTGATAPRGLARLATLIRAVRAENPNTILVDCGDTIQG
TPLEGVYQRALRAGNPPAPGDPMMRAMNLLGYDAMTVGNHEFNAGLANFNSARRDARFPWISANIAVATGGAERAFAPYI
VKTVAGVKVAVIGLTTPMVPLWEKPENLGAYRFTPPVEAVKSAIAKLRRDERPDLIVVTAHSGLGRNLQTNQDEETVENF
VHELARQMPELDAIVFGHSHQQLEGALVGNVLLVQPKNAAASLARIDFTFDGTLISKRSRLIPATAQTAAAPDLMALAAP
YEAATQRYLNTPVAHCDRELTATRAREEDSAIVDAIQRVQLYYAKADVSFTALFDPQVRIPRGEVTVRQIAALYPYDNEL
FGIEGTGKMVKDALENAARYFSAKPIFGFNYDMAEGVEYEIDRSRPEGDRIRNLRWKGRPLDPAQRLRIAINNYRAGGSG
GYDMFRGAKILWRSGDDIRDLLIRYYTERKSIPAEATNNWKLKN

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=548, Percent_Identity=25.5474452554745, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1790658, Length=597, Percent_Identity=29.4807370184255, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI1786687, Length=503, Percent_Identity=27.037773359841, Blast_Score=140, Evalue=2e-34,
Organism=Drosophila melanogaster, GI28573524, Length=495, Percent_Identity=23.030303030303, Blast_Score=78, Evalue=2e-14,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 57621; Mature: 57621

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: PS00785 5_NUCLEOTIDASE_1 ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTIRRLLPILLLSAGLLRPAAVTVTLLATTDMHGNLVPVDYVTGATAPRGLARLATLIR
CCHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCEEEEHHCCCCCCHHHHHHHHHHHH
AVRAENPNTILVDCGDTIQGTPLEGVYQRALRAGNPPAPGDPMMRAMNLLGYDAMTVGNH
HHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEECCC
EFNAGLANFNSARRDARFPWISANIAVATGGAERAFAPYIVKTVAGVKVAVIGLTTPMVP
CCCCCHHCCCHHHHCCCCCEEECEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCC
LWEKPENLGAYRFTPPVEAVKSAIAKLRRDERPDLIVVTAHSGLGRNLQTNQDEETVENF
CCCCCCCCCCEECCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHH
VHELARQMPELDAIVFGHSHQQLEGALVGNVLLVQPKNAAASLARIDFTFDGTLISKRSR
HHHHHHHCCCCEEEEECCCHHHHHHHEECCEEEEECCCHHHHEEEEEEEECCCEECCCCC
LIPATAQTAAAPDLMALAAPYEAATQRYLNTPVAHCDRELTATRAREEDSAIVDAIQRVQ
CCCCCHHHHCCCCHHHHHCCHHHHHHHHHCCCHHHHCHHHHHHHCCCCHHHHHHHHHHHH
LYYAKADVSFTALFDPQVRIPRGEVTVRQIAALYPYDNELFGIEGTGKMVKDALENAARY
HEEEECCCEEEEEECCCEECCCCCHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHH
FSAKPIFGFNYDMAEGVEYEIDRSRPEGDRIRNLRWKGRPLDPAQRLRIAINNYRAGGSG
HCCCCCCCCCCCHHCCCEEEECCCCCCCCHHCCCCCCCCCCCHHHHHEEEEECCCCCCCC
GYDMFRGAKILWRSGDDIRDLLIRYYTERKSIPAEATNNWKLKN
CHHHHCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDTIRRLLPILLLSAGLLRPAAVTVTLLATTDMHGNLVPVDYVTGATAPRGLARLATLIR
CCHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCEEEEHHCCCCCCHHHHHHHHHHHH
AVRAENPNTILVDCGDTIQGTPLEGVYQRALRAGNPPAPGDPMMRAMNLLGYDAMTVGNH
HHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEECCC
EFNAGLANFNSARRDARFPWISANIAVATGGAERAFAPYIVKTVAGVKVAVIGLTTPMVP
CCCCCHHCCCHHHHCCCCCEEECEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCC
LWEKPENLGAYRFTPPVEAVKSAIAKLRRDERPDLIVVTAHSGLGRNLQTNQDEETVENF
CCCCCCCCCCEECCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHH
VHELARQMPELDAIVFGHSHQQLEGALVGNVLLVQPKNAAASLARIDFTFDGTLISKRSR
HHHHHHHCCCCEEEEECCCHHHHHHHEECCEEEEECCCHHHHEEEEEEEECCCEECCCCC
LIPATAQTAAAPDLMALAAPYEAATQRYLNTPVAHCDRELTATRAREEDSAIVDAIQRVQ
CCCCCHHHHCCCCHHHHHCCHHHHHHHHHCCCHHHHCHHHHHHHCCCCHHHHHHHHHHHH
LYYAKADVSFTALFDPQVRIPRGEVTVRQIAALYPYDNELFGIEGTGKMVKDALENAARY
HEEEECCCEEEEEECCCEECCCCCHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHH
FSAKPIFGFNYDMAEGVEYEIDRSRPEGDRIRNLRWKGRPLDPAQRLRIAINNYRAGGSG
HCCCCCCCCCCCHHCCCEEEECCCCCCCCHHCCCCCCCCCCCHHHHHEEEEECCCCCCCC
GYDMFRGAKILWRSGDDIRDLLIRYYTERKSIPAEATNNWKLKN
CHHHHCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]