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The map label for this gene is tgt

Identifier: 116620399

GI number: 116620399

Start: 1574417

End: 1575556

Strand: Direct

Name: tgt

Synonym: Acid_1276

Alternate gene names: 116620399

Gene position: 1574417-1575556 (Clockwise)

Preceding gene: 116620393

Following gene: 116620400

Centisome position: 15.8

GC content: 60.96

Gene sequence:

>1140_bases
TTGCCTCAAATTTCCTTCGAAGTAGTTGCCGAATGTCCGCACACGCGCGCCCGCGCCGGCGTGCTGCACACCGCTCACGG
GGACATTGAAACGCCGGTGTTCATGCCGGTGGGGACGCAGGCCACCGTCAAGGGGCTGACGCAGCGCGATCTCGCCGAAG
ATCTCGGCGTCAAGATTCTGCTGAGCAACACCTATCACCTGTATCTTCGGCCGGGCCATGAATTAATCCGGCAAATGGGC
GGGCTGCACAAGTTCATGTCGTGGCCGAATGCGATCCTGACGGATTCCGGCGGATTTCAGGTGTTCAGCCTCAGCGGCCT
GCGCAAGATCCACGAACACGGCGTAGTGTTTCAATCGCACCTGAACGGCGACACCCACAAGTTCACGCCGGAATCCACGG
TGGACGTGCAACTGGCCTACGGCAGCGACATTCTGATGGTGCTCGACGAGTGTCCGGAATATCCGGTGAGCCACGAGTAC
GCGCGTGAGAGCATGCAGCGCACGGTGCGCTGGGCGCGCGAGGCGAACGAGCATTTCCTCGAGCGCATGAAGCAGATGCC
AACGCGGCACGCGCTGTTCCCCATCGTGCAGGGCTCCATGTTCACGGACCTGCGGCGCGAGTGCGCCACCGCGCTGGTGG
ACCTGGATACCGACGGGTACGCGATCGGCGGTCTATCGGTGGGCGAGCCGCGGCCGCTGAGCCTGGAGGTAGTGGAGGCG
ACGGAAGCGATCCTGCCCCGCACCAAGCCGCGCTATGCGATGGGGGTCGGAATGCCGGCGGAACTTCCGGAATACGTCGC
CCGCGGGATCGACATGATGGATTGCGTGCTGCCCTCGCGCAACGCGCGCAATGGCTACCTTTTCACCTCTGAAGGGCGGG
TAATCATCAAACATGCACGTTACAAGGACGACGAAGGGCCCCTCGATCCCAGGTGCGCCTGCTATACTTGTAAAAGCTAC
TCCCGAGCCTACCTTCGCCACCTGTTCCAGTCCGGGGAGATCCTCTTTTCAGTACTTGCGACACGTCACAACATCCAGCG
GTACCTTGACATCATGCGTGAGATTAGGCACGCTATAATATCGGGATCATTTCCAGACTACTTGAGGTGTGTGCAATCGA
GTCTGCACGACGCCGGGTAG

Upstream 100 bases:

>100_bases
CATAATTCAGCAGCCGGTGCTGAAATTCGATTGTACGCGAAGGTGAAGCCCCCGGCCGCGTTTCAAAATGGCTACCCACG
ATATCATGGGATGAATCCCA

Downstream 100 bases:

>100_bases
CGCTACGGTGTAGCGCGGCCACGTAGTAGTCCGTTGGTTTAAAACGTGATTTTCACTCTGTTTCTTCAATCGGCGCAGCC
CAATTCCCTGCTGGGATTCC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MPQISFEVVAECPHTRARAGVLHTAHGDIETPVFMPVGTQATVKGLTQRDLAEDLGVKILLSNTYHLYLRPGHELIRQMG
GLHKFMSWPNAILTDSGGFQVFSLSGLRKIHEHGVVFQSHLNGDTHKFTPESTVDVQLAYGSDILMVLDECPEYPVSHEY
ARESMQRTVRWAREANEHFLERMKQMPTRHALFPIVQGSMFTDLRRECATALVDLDTDGYAIGGLSVGEPRPLSLEVVEA
TEAILPRTKPRYAMGVGMPAELPEYVARGIDMMDCVLPSRNARNGYLFTSEGRVIIKHARYKDDEGPLDPRCACYTCKSY
SRAYLRHLFQSGEILFSVLATRHNIQRYLDIMREIRHAIISGSFPDYLRCVQSSLHDAG

Sequences:

>Translated_379_residues
MPQISFEVVAECPHTRARAGVLHTAHGDIETPVFMPVGTQATVKGLTQRDLAEDLGVKILLSNTYHLYLRPGHELIRQMG
GLHKFMSWPNAILTDSGGFQVFSLSGLRKIHEHGVVFQSHLNGDTHKFTPESTVDVQLAYGSDILMVLDECPEYPVSHEY
ARESMQRTVRWAREANEHFLERMKQMPTRHALFPIVQGSMFTDLRRECATALVDLDTDGYAIGGLSVGEPRPLSLEVVEA
TEAILPRTKPRYAMGVGMPAELPEYVARGIDMMDCVLPSRNARNGYLFTSEGRVIIKHARYKDDEGPLDPRCACYTCKSY
SRAYLRHLFQSGEILFSVLATRHNIQRYLDIMREIRHAIISGSFPDYLRCVQSSLHDAG
>Mature_378_residues
PQISFEVVAECPHTRARAGVLHTAHGDIETPVFMPVGTQATVKGLTQRDLAEDLGVKILLSNTYHLYLRPGHELIRQMGG
LHKFMSWPNAILTDSGGFQVFSLSGLRKIHEHGVVFQSHLNGDTHKFTPESTVDVQLAYGSDILMVLDECPEYPVSHEYA
RESMQRTVRWAREANEHFLERMKQMPTRHALFPIVQGSMFTDLRRECATALVDLDTDGYAIGGLSVGEPRPLSLEVVEAT
EAILPRTKPRYAMGVGMPAELPEYVARGIDMMDCVLPSRNARNGYLFTSEGRVIIKHARYKDDEGPLDPRCACYTCKSYS
RAYLRHLFQSGEILFSVLATRHNIQRYLDIMREIRHAIISGSFPDYLRCVQSSLHDAG

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=371, Percent_Identity=44.2048517520216, Blast_Score=308, Evalue=6e-84,
Organism=Escherichia coli, GI1786607, Length=353, Percent_Identity=50.1416430594901, Blast_Score=361, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17544680, Length=356, Percent_Identity=44.3820224719101, Blast_Score=289, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI25146502, Length=223, Percent_Identity=27.8026905829596, Blast_Score=78, Evalue=9e-15,
Organism=Drosophila melanogaster, GI19920510, Length=368, Percent_Identity=44.5652173913043, Blast_Score=319, Evalue=2e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_SOLUE (Q029K6)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_822555.1
- ProteinModelPortal:   Q029K6
- SMR:   Q029K6
- STRING:   Q029K6
- GeneID:   4431904
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_1276
- NMPDR:   fig|234267.9.peg.1217
- eggNOG:   COG0343
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- PhylomeDB:   Q029K6
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 42674; Mature: 42543

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: ACT_SITE 95-95 BINDING 96-96

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQISFEVVAECPHTRARAGVLHTAHGDIETPVFMPVGTQATVKGLTQRDLAEDLGVKIL
CCCCCHHHHHCCCCCHHHHCEEEECCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCEEEE
LSNTYHLYLRPGHELIRQMGGLHKFMSWPNAILTDSGGFQVFSLSGLRKIHEHGVVFQSH
EECCEEEEECCHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHCCCEEEEC
LNGDTHKFTPESTVDVQLAYGSDILMVLDECPEYPVSHEYARESMQRTVRWAREANEHFL
CCCCCCCCCCCCCEEEEEEECCCEEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ERMKQMPTRHALFPIVQGSMFTDLRRECATALVDLDTDGYAIGGLSVGEPRPLSLEVVEA
HHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHEECCCCCEEECCCCCCCCCCCEEEHHHH
TEAILPRTKPRYAMGVGMPAELPEYVARGIDMMDCVLPSRNARNGYLFTSEGRVIIKHAR
HHHHCCCCCCCCEECCCCCHHHHHHHHCCCCHHHHHCCCCCCCCCEEEECCCEEEEEECC
YKDDEGPLDPRCACYTCKSYSRAYLRHLFQSGEILFSVLATRHNIQRYLDIMREIRHAII
CCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGSFPDYLRCVQSSLHDAG
CCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PQISFEVVAECPHTRARAGVLHTAHGDIETPVFMPVGTQATVKGLTQRDLAEDLGVKIL
CCCCHHHHHCCCCCHHHHCEEEECCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCEEEE
LSNTYHLYLRPGHELIRQMGGLHKFMSWPNAILTDSGGFQVFSLSGLRKIHEHGVVFQSH
EECCEEEEECCHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHCCCEEEEC
LNGDTHKFTPESTVDVQLAYGSDILMVLDECPEYPVSHEYARESMQRTVRWAREANEHFL
CCCCCCCCCCCCCEEEEEEECCCEEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ERMKQMPTRHALFPIVQGSMFTDLRRECATALVDLDTDGYAIGGLSVGEPRPLSLEVVEA
HHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHEECCCCCEEECCCCCCCCCCCEEEHHHH
TEAILPRTKPRYAMGVGMPAELPEYVARGIDMMDCVLPSRNARNGYLFTSEGRVIIKHAR
HHHHCCCCCCCCEECCCCCHHHHHHHHCCCCHHHHHCCCCCCCCCEEEECCCEEEEEECC
YKDDEGPLDPRCACYTCKSYSRAYLRHLFQSGEILFSVLATRHNIQRYLDIMREIRHAII
CCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGSFPDYLRCVQSSLHDAG
CCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA