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The map label for this gene is oprF [H]

Identifier: 116620394

GI number: 116620394

Start: 1569006

End: 1570643

Strand: Reverse

Name: oprF [H]

Synonym: Acid_1271

Alternate gene names: 116620394

Gene position: 1570643-1569006 (Counterclockwise)

Preceding gene: 116620395

Following gene: 116620392

Centisome position: 15.76

GC content: 62.52

Gene sequence:

>1638_bases
ATGAAAAATCTTACGATGCTGTACCTGGCAACCTTTGGTGCGCTTGTGGCTCAGGTCCCTAACCCGACCCAAAAGCAGAC
GCCTGTCGATGCCAATACAGGGCAACCCATGCCTATCTTCCGGGTGACCGTTGTTTCCCGGACCACCAAGGCAATCAATT
ACAACCACCGCAACGGTTCCACCCCAATCGATTTCCGAGGCACCGAACTGATGCCCGCAGCCCGCGGCCACGCCACCGTG
GATAGCAAAATGGGCTCCACGAAAATTGAGACTCGGCTGGAAAAAATGACTCCGGCCAGCCAGTATGGGCCGGAATATAT
GACCTACGTGTTGTGGGCGATCACGCCCGAAGGCCGCGCCAATAACCTCGGCGAAGTCGTGTTGCAGGGCGATCACGCCA
ACCTGCTCTCCACGACCGACCTGCAGAGCTTCGGCCTCATTGTCACCGCCGAACCTTACTTCGCCGTGACCCAGCCGAGC
GATGTCGTGGTCGCGGAGAACTTCGTCACCAAGGAAACCGAAGGCACGATCCAGCAGGTGGATGCCAAATACGAACTCCT
GAAGCGCGGCCAGTACGTCTTCAACCGCTCCATGTACAAACCGCCGCGGGTTGATCCCAAGGGCCCGCTGCAGCTCGGAC
AGGCCGAGAACGCGGTAGAAATCGCGCGTCTCGCCGGCGCTGACAAGTATGCGGCCGACACCTTTCAGAAAGCCGTGGTC
GATCTGCAAAATGCCGAGGATTTCCTGAATGGCAAGAGCAAGGATCGCAAACGCGCCGAAACCAACGCTCGCGAAGCCAC
CCAGATGGCCGAGGACGCCCGCATCATCACTTTCCGCAAGATGAATGAGGAAGCCCTGGCGAACGAGCGCGCCGCCGCGG
CCGCACGCGAAGCCGACGCCAAGGAAAAGGCCCGCCTCGCCGACGAACAGGCTCGCATGGATCAGCAGCGCCGCGCCCAG
GCCGAGGCCGATCAGCGCGCCTCGGAGACTGCCCGCCGACAGGCCGAGCAGGCCAAGTTGGAAGCCGATAATGCCCGCGC
TTCCGCCGAGAAGGCCGCCGCCGATGCCGCGCGCGAACGCGCCGCGGCCGACGCCGCGCGCCTCGCCGCCGAGCAGCAGC
AGCAGGCCGCGATGGCCGAAGCCGACCGCGCCAAACAGGGCATGCAGAAGGCTGAAGCCGAGAAGGCCGAACTCCGCGAG
CGCCTGCGCAATCAGTTGAACATGATTCTCGAAACCCGCGAAACCGCGCGCGGCCTCATCATCAACATCTCCGACGTGCT
CTTCGATTTCAACAAGTACACGCTCAAGCCGGGCGCTCGCGAAAAACTCGCCAAGGTCTCCGGTATCCTGCTCGCCTATC
CCGGCCTGAACATCAAGGTCGAAGGCCACACGGATAGCATCGGCAGCGACGAATACAACCAGAAGCTCTCCGAATCGCGT
GCCAGCTCCGTGCGCGATTACCTCACCTCGCAGAACGTTCCCGCGGATTCGGTGAGCGCCGTGGGCTTGGGCAAATCCGA
CCCCGTCGCCAGCAACGATACGGCTGCCGGACGCCAACGCAACCGCCGCGTCGAACTCGTCGTCTCGGGCGAGCCGATCG
GCATCGGCGATATCAGCGGGCCTTCCTCTAAGCAGTAG

Upstream 100 bases:

>100_bases
CCTCCGGCCGCTTCCGGCTCTCCAGAGTGATCTGTTTTGTTGTTTGCTGGCGCACATCGGGCACCCGCGTGCTCCTCCAC
CCCCCTGAGGAGCCGCGAAC

Downstream 100 bases:

>100_bases
CCACCGAAAAAGGGACAGGCTGGATCACCGGCCTGTCCCGGTTCTCAAAACAGCTTTAGCCGCAAAAACTTCTTGGGGTT
CTCCCGAAAATCCTTCAGTG

Product: OmpA/MotB domain-containing protein

Products: NA

Alternate protein names: Root adhesin [H]

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MKNLTMLYLATFGALVAQVPNPTQKQTPVDANTGQPMPIFRVTVVSRTTKAINYNHRNGSTPIDFRGTELMPAARGHATV
DSKMGSTKIETRLEKMTPASQYGPEYMTYVLWAITPEGRANNLGEVVLQGDHANLLSTTDLQSFGLIVTAEPYFAVTQPS
DVVVAENFVTKETEGTIQQVDAKYELLKRGQYVFNRSMYKPPRVDPKGPLQLGQAENAVEIARLAGADKYAADTFQKAVV
DLQNAEDFLNGKSKDRKRAETNAREATQMAEDARIITFRKMNEEALANERAAAAAREADAKEKARLADEQARMDQQRRAQ
AEADQRASETARRQAEQAKLEADNARASAEKAAADAARERAAADAARLAAEQQQQAAMAEADRAKQGMQKAEAEKAELRE
RLRNQLNMILETRETARGLIINISDVLFDFNKYTLKPGAREKLAKVSGILLAYPGLNIKVEGHTDSIGSDEYNQKLSESR
ASSVRDYLTSQNVPADSVSAVGLGKSDPVASNDTAAGRQRNRRVELVVSGEPIGIGDISGPSSKQ

Sequences:

>Translated_545_residues
MKNLTMLYLATFGALVAQVPNPTQKQTPVDANTGQPMPIFRVTVVSRTTKAINYNHRNGSTPIDFRGTELMPAARGHATV
DSKMGSTKIETRLEKMTPASQYGPEYMTYVLWAITPEGRANNLGEVVLQGDHANLLSTTDLQSFGLIVTAEPYFAVTQPS
DVVVAENFVTKETEGTIQQVDAKYELLKRGQYVFNRSMYKPPRVDPKGPLQLGQAENAVEIARLAGADKYAADTFQKAVV
DLQNAEDFLNGKSKDRKRAETNAREATQMAEDARIITFRKMNEEALANERAAAAAREADAKEKARLADEQARMDQQRRAQ
AEADQRASETARRQAEQAKLEADNARASAEKAAADAARERAAADAARLAAEQQQQAAMAEADRAKQGMQKAEAEKAELRE
RLRNQLNMILETRETARGLIINISDVLFDFNKYTLKPGAREKLAKVSGILLAYPGLNIKVEGHTDSIGSDEYNQKLSESR
ASSVRDYLTSQNVPADSVSAVGLGKSDPVASNDTAAGRQRNRRVELVVSGEPIGIGDISGPSSKQ
>Mature_545_residues
MKNLTMLYLATFGALVAQVPNPTQKQTPVDANTGQPMPIFRVTVVSRTTKAINYNHRNGSTPIDFRGTELMPAARGHATV
DSKMGSTKIETRLEKMTPASQYGPEYMTYVLWAITPEGRANNLGEVVLQGDHANLLSTTDLQSFGLIVTAEPYFAVTQPS
DVVVAENFVTKETEGTIQQVDAKYELLKRGQYVFNRSMYKPPRVDPKGPLQLGQAENAVEIARLAGADKYAADTFQKAVV
DLQNAEDFLNGKSKDRKRAETNAREATQMAEDARIITFRKMNEEALANERAAAAAREADAKEKARLADEQARMDQQRRAQ
AEADQRASETARRQAEQAKLEADNARASAEKAAADAARERAAADAARLAAEQQQQAAMAEADRAKQGMQKAEAEKAELRE
RLRNQLNMILETRETARGLIINISDVLFDFNKYTLKPGAREKLAKVSGILLAYPGLNIKVEGHTDSIGSDEYNQKLSESR
ASSVRDYLTSQNVPADSVSAVGLGKSDPVASNDTAAGRQRNRRVELVVSGEPIGIGDISGPSSKQ

Specific function: Known to stabilize the outer membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI1787191, Length=118, Percent_Identity=47.4576271186441, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI48994946, Length=139, Percent_Identity=36.6906474820144, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788957, Length=118, Percent_Identity=36.4406779661017, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1786962, Length=102, Percent_Identity=37.2549019607843, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1480 Molecules/Cell In: Growth Phase, Mi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 59574; Mature: 59574

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLTMLYLATFGALVAQVPNPTQKQTPVDANTGQPMPIFRVTVVSRTTKAINYNHRNGS
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHEECCCCCCC
TPIDFRGTELMPAARGHATVDSKMGSTKIETRLEKMTPASQYGPEYMTYVLWAITPEGRA
CCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHCCCHHHCCCCEEEEEEEEECCCCCC
NNLGEVVLQGDHANLLSTTDLQSFGLIVTAEPYFAVTQPSDVVVAENFVTKETEGTIQQV
CCCCEEEEECCCCCCEECCCHHHCCEEEECCCEEEEECCCCEEEECCHHCCCCCCHHHHH
DAKYELLKRGQYVFNRSMYKPPRVDPKGPLQLGQAENAVEIARLAGADKYAADTFQKAVV
HHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHH
DLQNAEDFLNGKSKDRKRAETNAREATQMAEDARIITFRKMNEEALANERAAAAAREADA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHCCH
KEKARLADEQARMDQQRRAQAEADQRASETARRQAEQAKLEADNARASAEKAAADAARER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
AAADAARLAAEQQQQAAMAEADRAKQGMQKAEAEKAELRERLRNQLNMILETRETARGLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
INISDVLFDFNKYTLKPGAREKLAKVSGILLAYPGLNIKVEGHTDSIGSDEYNQKLSESR
EEHHHHHHCCCCEEECCCHHHHHHHHHCEEEECCCCEEEECCCCCCCCCHHHHHHHHHHH
ASSVRDYLTSQNVPADSVSAVGLGKSDPVASNDTAAGRQRNRRVELVVSGEPIGIGDISG
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCC
PSSKQ
CCCCC
>Mature Secondary Structure
MKNLTMLYLATFGALVAQVPNPTQKQTPVDANTGQPMPIFRVTVVSRTTKAINYNHRNGS
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHEECCCCCCC
TPIDFRGTELMPAARGHATVDSKMGSTKIETRLEKMTPASQYGPEYMTYVLWAITPEGRA
CCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHCCCHHHCCCCEEEEEEEEECCCCCC
NNLGEVVLQGDHANLLSTTDLQSFGLIVTAEPYFAVTQPSDVVVAENFVTKETEGTIQQV
CCCCEEEEECCCCCCEECCCHHHCCEEEECCCEEEEECCCCEEEECCHHCCCCCCHHHHH
DAKYELLKRGQYVFNRSMYKPPRVDPKGPLQLGQAENAVEIARLAGADKYAADTFQKAVV
HHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHH
DLQNAEDFLNGKSKDRKRAETNAREATQMAEDARIITFRKMNEEALANERAAAAAREADA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHCCH
KEKARLADEQARMDQQRRAQAEADQRASETARRQAEQAKLEADNARASAEKAAADAARER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
AAADAARLAAEQQQQAAMAEADRAKQGMQKAEAEKAELRERLRNQLNMILETRETARGLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
INISDVLFDFNKYTLKPGAREKLAKVSGILLAYPGLNIKVEGHTDSIGSDEYNQKLSESR
EEHHHHHHCCCCEEECCCHHHHHHHHHCEEEECCCCEEEECCCCCCCCCHHHHHHHHHHH
ASSVRDYLTSQNVPADSVSAVGLGKSDPVASNDTAAGRQRNRRVELVVSGEPIGIGDISG
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCC
PSSKQ
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1538702 [H]