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The map label for this gene is dfrA [H]

Identifier: 116620384

GI number: 116620384

Start: 1560517

End: 1561488

Strand: Direct

Name: dfrA [H]

Synonym: Acid_1261

Alternate gene names: 116620384

Gene position: 1560517-1561488 (Clockwise)

Preceding gene: 116620383

Following gene: 116620385

Centisome position: 15.66

GC content: 65.84

Gene sequence:

>972_bases
ATGAAACCGGCTCTGGTAACGGGCGCTTCCGGATTTCTCGGCTGGCATGTGGCGCGCGTGCTGGTGGAGCGCGGACTCCA
CGTCCGGGCGCTGGTCCGACCGGGCAGTAAGGTCGTCGGCATCGACGTGGAGTGTGTCACGGGCGATTTGCGCGATCCCG
CCTCGCTGGCCCTTGCAGTCAAAGGTTGCGGCCTTGTGTTTCACGTGGCGGCGGATTACCGTCTGTGGGCCAAGGACCCC
ACGGAGTTGTACCGGTCGAATGTGGACGGCACGCGCAATCTGCTGGAGGCGGCGCGCCAGGCGGGCGTGGAGCGAACGGT
CTATACCAGCACGGTGGGCTGTATCGGCATGCCGCGGGACGGCATTGGCGACGAAGCGCAGCCGGTGAAACTAGCCCACA
TGGCGGGGGACTACAAGCGCTCCAAATTCCTCGCGGAGAAGGTCGCGCTGGAATTCGCGCGCGCCGGGCAGCCGGTGGTG
ATCGTAAACCCCACGGCTCCGCTTGGCGATCATGACGTGAAGCCGACGCCCACGGGCAAGATCGTCCTCGATTTTCTGAA
GGGCGACATGCCGGCATTCATCGATACCGGGCTAAACGTAGTGGACGTGCGCGATACAGCGGAGGGACACTGGCAGGCGT
GCGAGCGTGGACGCTCCGGCGAGCGCTACATCCTGGGCTCGGAGAATCTTACGCTGGCGCAGATTCTGCAAAAGCTGGCG
GCAATCACGGGCAGGAAAGCGCCCACCCTGCAACTGCCGTACGCACTGGCCTATTGCGCGGGCGCGTGCAGCACAGCCTG
GGCCGCCGTCACCGGCCGTCCGCCCCGGGTGCCGCTCGAAGCCGTGCGGATGGCGAAGAAGAAGATGTGGGTGAGCCACG
ATAAGGCCGCGCGTGAACTGGGCTTTCAGCCGGGTCCGGCCGAGAAGGCGCTGCGGCACGCAGTGGACTGGTTCCGCCAA
ACCGCTGCCTGA

Upstream 100 bases:

>100_bases
GTGGGGCAGGCATTTTGCCTGCAGCCGCCTTTCAGGCGGCTCCTTCATCCAAGCCGTTTCCCAGTTTGCGGCTTGCGGCA
CTGTGGGGCAGACTCCTGCT

Downstream 100 bases:

>100_bases
TGAAACTACTCTTCGTGGCGGCAGATGCGATGGAGTTCCCCGGCATTCTGGCGCGTTCGCGCGAAAGCCGGCGTCCGGCA
GTCGCGGTGGATTGGGCGCG

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKPALVTGASGFLGWHVARVLVERGLHVRALVRPGSKVVGIDVECVTGDLRDPASLALAVKGCGLVFHVAADYRLWAKDP
TELYRSNVDGTRNLLEAARQAGVERTVYTSTVGCIGMPRDGIGDEAQPVKLAHMAGDYKRSKFLAEKVALEFARAGQPVV
IVNPTAPLGDHDVKPTPTGKIVLDFLKGDMPAFIDTGLNVVDVRDTAEGHWQACERGRSGERYILGSENLTLAQILQKLA
AITGRKAPTLQLPYALAYCAGACSTAWAAVTGRPPRVPLEAVRMAKKKMWVSHDKAARELGFQPGPAEKALRHAVDWFRQ
TAA

Sequences:

>Translated_323_residues
MKPALVTGASGFLGWHVARVLVERGLHVRALVRPGSKVVGIDVECVTGDLRDPASLALAVKGCGLVFHVAADYRLWAKDP
TELYRSNVDGTRNLLEAARQAGVERTVYTSTVGCIGMPRDGIGDEAQPVKLAHMAGDYKRSKFLAEKVALEFARAGQPVV
IVNPTAPLGDHDVKPTPTGKIVLDFLKGDMPAFIDTGLNVVDVRDTAEGHWQACERGRSGERYILGSENLTLAQILQKLA
AITGRKAPTLQLPYALAYCAGACSTAWAAVTGRPPRVPLEAVRMAKKKMWVSHDKAARELGFQPGPAEKALRHAVDWFRQ
TAA
>Mature_323_residues
MKPALVTGASGFLGWHVARVLVERGLHVRALVRPGSKVVGIDVECVTGDLRDPASLALAVKGCGLVFHVAADYRLWAKDP
TELYRSNVDGTRNLLEAARQAGVERTVYTSTVGCIGMPRDGIGDEAQPVKLAHMAGDYKRSKFLAEKVALEFARAGQPVV
IVNPTAPLGDHDVKPTPTGKIVLDFLKGDMPAFIDTGLNVVDVRDTAEGHWQACERGRSGERYILGSENLTLAQILQKLA
AITGRKAPTLQLPYALAYCAGACSTAWAAVTGRPPRVPLEAVRMAKKKMWVSHDKAARELGFQPGPAEKALRHAVDWFRQ
TAA

Specific function: Galactose metabolism; third step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Homo sapiens, GI116268111, Length=353, Percent_Identity=26.628895184136, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI193211614, Length=341, Percent_Identity=26.099706744868, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI8393516, Length=341, Percent_Identity=26.099706744868, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71987463, Length=275, Percent_Identity=30.5454545454545, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17570557, Length=336, Percent_Identity=25, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 34820; Mature: 34820

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPALVTGASGFLGWHVARVLVERGLHVRALVRPGSKVVGIDVECVTGDLRDPASLALAV
CCCCEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEEEEEEECCCCCCHHHEEEE
KGCGLVFHVAADYRLWAKDPTELYRSNVDGTRNLLEAARQAGVERTVYTSTVGCIGMPRD
ECCCEEEEEECCCEEECCCHHHHHHCCCCHHHHHHHHHHHCCCCCEEEHHHHCCCCCCCC
GIGDEAQPVKLAHMAGDYKRSKFLAEKVALEFARAGQPVVIVNPTAPLGDHDVKPTPTGK
CCCCCCCCEEHHHHHCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHH
IVLDFLKGDMPAFIDTGLNVVDVRDTAEGHWQACERGRSGERYILGSENLTLAQILQKLA
HHHEEHHCCCCHHHHCCCEEEEECCCCCHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHH
AITGRKAPTLQLPYALAYCAGACSTAWAAVTGRPPRVPLEAVRMAKKKMWVSHDKAAREL
HHHCCCCCCEECHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
GFQPGPAEKALRHAVDWFRQTAA
CCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKPALVTGASGFLGWHVARVLVERGLHVRALVRPGSKVVGIDVECVTGDLRDPASLALAV
CCCCEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEEEEEEECCCCCCHHHEEEE
KGCGLVFHVAADYRLWAKDPTELYRSNVDGTRNLLEAARQAGVERTVYTSTVGCIGMPRD
ECCCEEEEEECCCEEECCCHHHHHHCCCCHHHHHHHHHHHCCCCCEEEHHHHCCCCCCCC
GIGDEAQPVKLAHMAGDYKRSKFLAEKVALEFARAGQPVVIVNPTAPLGDHDVKPTPTGK
CCCCCCCCEEHHHHHCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHH
IVLDFLKGDMPAFIDTGLNVVDVRDTAEGHWQACERGRSGERYILGSENLTLAQILQKLA
HHHEEHHCCCCHHHHCCCEEEEECCCCCHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHH
AITGRKAPTLQLPYALAYCAGACSTAWAAVTGRPPRVPLEAVRMAKKKMWVSHDKAAREL
HHHCCCCCCEECHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
GFQPGPAEKALRHAVDWFRQTAA
CCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]