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The map label for this gene is ispG

Identifier: 116620316

GI number: 116620316

Start: 1473671

End: 1474918

Strand: Direct

Name: ispG

Synonym: Acid_1193

Alternate gene names: 116620316

Gene position: 1473671-1474918 (Clockwise)

Preceding gene: 116620315

Following gene: 116620317

Centisome position: 14.79

GC content: 63.94

Gene sequence:

>1248_bases
ATGGCAGTCAAGCCTCGCCGTAATGCAGTCGTTGTCGATGTCGGAGGAGTGAAAGTCGGCGGCACGCACCCCATCGTCGT
GCAGTCCATGACCAACACCGACACTGCCGATGTCGCCTCCACGGTCAACCAGGTAATGGCGCTCGCCCGCGCCGGATCCG
AACTCGTCCGCGTCACCGTCAACACCGAAGCCGCCGCCGCCGCCGTCCCCAAGATCGTCGATGCCTGCGCCGCTTTCGGC
GTTCGCGTCCCCATCATCGGCGACTTCCACTACAACGGTCACCTCCTCCTCAAGAAGTACCCGGAGTGCGCCCGCGCCCT
CGCCAAGTACCGCATCAATCCCGGCAACTCCGACATCGGCCGCAAGACCGACGACAACTTCCGCACCATGATCGAAGTCG
CCATTGAGAATCGTAAGCCCGTCCGCATCGGTGTCAACTGGGGCTCCCTCGATTCCGCGCTGCTCACCAGAATGATGGAC
GAGAACTCCCTTCTCCCCGAGCCCAAGGACGCCCGCGAAGTCACGCTGGACGCGATGGTCGCCAGCGCCGTCCAGTCCGC
CCGGGCCGCGGAGCGCCACGGCCTCGCCCACGATCAGATCATCCTCAGCGCCAAAGTCAGCGGCGTCCAGGATCTGATCG
ATGTCTACCGCGCCCTCGCCGCCGTCTGCGATTATCCGCTCCACCTCGGCCTCACCGAAGCCGGCCTCGGCAGCAAGGGC
ATCGTTGCCACCACAGCCGCCCTTGCCCCCGTCCTCCAGGACGGCATCGGCGACACAATCCGCACCTCGCTCACCCCAAT
GCCCAATGGCGACCGCACCGAAGAGGTCATCGTCAGCCAGCAGATTCTGCAGTCGCTCGGCATCCGCAGCTTCACTCCGC
AGGTCACCGCCTGCCCTGGCTGTGGCCGCACCACAAGCACCTTCTTCCAGGAAATGGCCGATGAGATCCAGACCTACCTG
CGCGTGCAAATGCCCAGCTGGAAGGCCACTCATCCCGGCGTCGAAACCATGAAGGTTGCCGTCATGGGCTGCGTGGTCAA
TGGACCCGGCGAGTCCAAACACTCCAACATCGGCATCTCGCTCCCCGGCACCTTCGAGGAACCCAAAGCCCCCGTCTACG
TCGATGGCCGCCTCATGACCACCCTCAAAGGCGACCGCATCGTCGCGGAGTTTTTGGAGATTCTGAACGAGTATGTGGAT
ACCCACTACGCCATCGCCGGTGAGCCCGAACTGGTGTCGGGCGATTAG

Upstream 100 bases:

>100_bases
CTCCCTTCCTAGCGAGCCGCAGCGCGAACGGCGACCGTAAAGGAGCGATCTCTTTCCAGATACCCTGCTAAAATCAAATT
TCCACCCTGGAGGGATCTTC

Downstream 100 bases:

>100_bases
CCCGCGCCATGTCCAAGCGCTTCACTCTCGCCGAGGCCCAGAGCCTCATCCCCGAAGTCGATCGTTTGCTTCGCCAAGCC
TTGGACAGCAAGAGCGCCTA

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MAVKPRRNAVVVDVGGVKVGGTHPIVVQSMTNTDTADVASTVNQVMALARAGSELVRVTVNTEAAAAAVPKIVDACAAFG
VRVPIIGDFHYNGHLLLKKYPECARALAKYRINPGNSDIGRKTDDNFRTMIEVAIENRKPVRIGVNWGSLDSALLTRMMD
ENSLLPEPKDAREVTLDAMVASAVQSARAAERHGLAHDQIILSAKVSGVQDLIDVYRALAAVCDYPLHLGLTEAGLGSKG
IVATTAALAPVLQDGIGDTIRTSLTPMPNGDRTEEVIVSQQILQSLGIRSFTPQVTACPGCGRTTSTFFQEMADEIQTYL
RVQMPSWKATHPGVETMKVAVMGCVVNGPGESKHSNIGISLPGTFEEPKAPVYVDGRLMTTLKGDRIVAEFLEILNEYVD
THYAIAGEPELVSGD

Sequences:

>Translated_415_residues
MAVKPRRNAVVVDVGGVKVGGTHPIVVQSMTNTDTADVASTVNQVMALARAGSELVRVTVNTEAAAAAVPKIVDACAAFG
VRVPIIGDFHYNGHLLLKKYPECARALAKYRINPGNSDIGRKTDDNFRTMIEVAIENRKPVRIGVNWGSLDSALLTRMMD
ENSLLPEPKDAREVTLDAMVASAVQSARAAERHGLAHDQIILSAKVSGVQDLIDVYRALAAVCDYPLHLGLTEAGLGSKG
IVATTAALAPVLQDGIGDTIRTSLTPMPNGDRTEEVIVSQQILQSLGIRSFTPQVTACPGCGRTTSTFFQEMADEIQTYL
RVQMPSWKATHPGVETMKVAVMGCVVNGPGESKHSNIGISLPGTFEEPKAPVYVDGRLMTTLKGDRIVAEFLEILNEYVD
THYAIAGEPELVSGD
>Mature_414_residues
AVKPRRNAVVVDVGGVKVGGTHPIVVQSMTNTDTADVASTVNQVMALARAGSELVRVTVNTEAAAAAVPKIVDACAAFGV
RVPIIGDFHYNGHLLLKKYPECARALAKYRINPGNSDIGRKTDDNFRTMIEVAIENRKPVRIGVNWGSLDSALLTRMMDE
NSLLPEPKDAREVTLDAMVASAVQSARAAERHGLAHDQIILSAKVSGVQDLIDVYRALAAVCDYPLHLGLTEAGLGSKGI
VATTAALAPVLQDGIGDTIRTSLTPMPNGDRTEEVIVSQQILQSLGIRSFTPQVTACPGCGRTTSTFFQEMADEIQTYLR
VQMPSWKATHPGVETMKVAVMGCVVNGPGESKHSNIGISLPGTFEEPKAPVYVDGRLMTTLKGDRIVAEFLEILNEYVDT
HYAIAGEPELVSGD

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=376, Percent_Identity=38.031914893617, Blast_Score=188, Evalue=5e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_SOLUE (Q029T9)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_822472.1
- STRING:   Q029T9
- GeneID:   4430636
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_1193
- NMPDR:   fig|234267.9.peg.1132
- eggNOG:   COG0821
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- PhylomeDB:   Q029T9
- ProtClustDB:   PRK00366
- HAMAP:   MF_00159
- InterPro:   IPR016425
- InterPro:   IPR004588
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE

EC number: =1.17.7.1

Molecular weight: Translated: 44369; Mature: 44238

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVKPRRNAVVVDVGGVKVGGTHPIVVQSMTNTDTADVASTVNQVMALARAGSELVRVTV
CCCCCCCCEEEEEECCEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEEE
NTEAAAAAVPKIVDACAAFGVRVPIIGDFHYNGHLLLKKYPECARALAKYRINPGNSDIG
CCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEEECCHHHHHHHHHEECCCCCCCCC
RKTDDNFRTMIEVAIENRKPVRIGVNWGSLDSALLTRMMDENSLLPEPKDAREVTLDAMV
CCCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
ASAVQSARAAERHGLAHDQIILSAKVSGVQDLIDVYRALAAVCDYPLHLGLTEAGLGSKG
HHHHHHHHHHHHCCCCHHHEEEEHHHCCHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCC
IVATTAALAPVLQDGIGDTIRTSLTPMPNGDRTEEVIVSQQILQSLGIRSFTPQVTACPG
HHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEECCC
CGRTTSTFFQEMADEIQTYLRVQMPSWKATHPGVETMKVAVMGCVVNGPGESKHSNIGIS
CCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHEEEEEEEECCCCCCCCCCEEEE
LPGTFEEPKAPVYVDGRLMTTLKGDRIVAEFLEILNEYVDTHYAIAGEPELVSGD
CCCCCCCCCCCEEECCEEEEEECCCHHHHHHHHHHHHHHHCCEEECCCCCCCCCC
>Mature Secondary Structure 
AVKPRRNAVVVDVGGVKVGGTHPIVVQSMTNTDTADVASTVNQVMALARAGSELVRVTV
CCCCCCCEEEEEECCEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEEE
NTEAAAAAVPKIVDACAAFGVRVPIIGDFHYNGHLLLKKYPECARALAKYRINPGNSDIG
CCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEEECCHHHHHHHHHEECCCCCCCCC
RKTDDNFRTMIEVAIENRKPVRIGVNWGSLDSALLTRMMDENSLLPEPKDAREVTLDAMV
CCCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
ASAVQSARAAERHGLAHDQIILSAKVSGVQDLIDVYRALAAVCDYPLHLGLTEAGLGSKG
HHHHHHHHHHHHCCCCHHHEEEEHHHCCHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCC
IVATTAALAPVLQDGIGDTIRTSLTPMPNGDRTEEVIVSQQILQSLGIRSFTPQVTACPG
HHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEECCC
CGRTTSTFFQEMADEIQTYLRVQMPSWKATHPGVETMKVAVMGCVVNGPGESKHSNIGIS
CCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHEEEEEEEECCCCCCCCCCEEEE
LPGTFEEPKAPVYVDGRLMTTLKGDRIVAEFLEILNEYVDTHYAIAGEPELVSGD
CCCCCCCCCCCEEECCEEEEEECCCHHHHHHHHHHHHHHHCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA