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The map label for this gene is dac [H]

Identifier: 116620153

GI number: 116620153

Start: 1270758

End: 1272266

Strand: Direct

Name: dac [H]

Synonym: Acid_1026

Alternate gene names: 116620153

Gene position: 1270758-1272266 (Clockwise)

Preceding gene: 116620152

Following gene: 116620154

Centisome position: 12.75

GC content: 66.93

Gene sequence:

>1509_bases
ATGACCGCGAAGCAGCGCGCCGCTGTCGCGACATTCTTACTCGCAGCCCTCGCATCCCAGTGCGCCGCCGCCGGATTGCC
GGAGGCCATTCAGAAATTGATCGATTCCTCCCCTGCCGCGCGCACCGCCTTCTGGGGAATCCAGATCGTCGATCTCGCCA
GCGGCAAGACCCTCTACGAGCTCAATCCTGATCACTACTTCGTCCCCGCCTCGAACGCCAAGCTCTTCAGTACCGCGCTC
GCACTGACCCGCCTCGGCCCCGACTACACCTTCCAGACCCGCGTCTTCGCCGACGCCCCGCCCGATTCCCAGGGCCGCAT
CTCTGGCGACCTGCGCCTCATCGGCGGCGGCGATCCGAACCTCTCCGCGCGCGCCATCCCTTATCGAATGGGCCCCCCCA
CCGGCAATCCCCTCGCCGCCATCGAAGACCTCGCCGACCAGCTCGCCGCCCGCGGCATCCGCATCGTCTCCGGCGACATC
GTCGGCGACGACACCTGGTACGTCTGGCAGCCCTACGCGGTCGGCTGGGCGATCGACGATCCACAATCCGACGATGGCCC
GCCCATCTCCGCCCTCACTCTCTCCGATAACGCCCTCAACCTTTCGGTGCATCCGGGCGCCGCCGTCGGCGAGGTCGCAG
AGCTCGCCTTCAGCCCGCCTTTGGAGTTCTACCGCATCGAGAATCGCGTGCGCACCGTCGCCGCCGGAGGCGAGCGCCGC
GTTCACTTTCACCGCATCCCCGGCAGCCGTGACGCCGTCCTGTGGGGAACCATCCCGCTGCGCGACCGCGGCCAGGACAT
GCTGACCTCTGTCGAAGATCCCGCCGAGTTCGCCGCCGAGGCCCTGCGCAACGCGCTCGAGGCGCGCGGCATCACGGTCG
AAGGCGCCGCCCGCTCACAGCACCTCTTCCCCAATCAGCTCGCCGATCTCACACAAGCGCCTCCCTCGGAGGCGGTTGGC
GGAGTGGAACTCGCGAATCGCTCCTCCGCCCGCCTTGTCGAAGACCTGCGCGTAACCGCCAAGGTCAGCCAGAACCTGCA
CGCCGAACTCCTGCTTCGCGCCGTGGGCCGCGCCCGCCGCAACGTGGGCAGCTTCGAGGCGGGTATGACCGAGATGAAGA
TTTTTCTCGCCGACGCAGGCGTGGAGCCGACCGGCTATAACCTGCTCGATGGCTCCGGCCTCAGCCGCCTGGACCTGGTC
ACGCCCGCCACCGTCGTCAGACTCCTCCGCTTCATGTACGATTCGCCGGCGCGCGATAACTGGCTTTCCATCTTTCCCAT
CGGCGGCCAGGATGGCACCCTCAGCACACGTTTCGGGGGCACTCCGGCTGCCGGCCGCGTCCTCGCCAAGACCGGCTCCC
TCTCCCACGTCAGCGCGCTCTCCGGCTATATCCAGCGCCTGGATGGAACCTGGCTGGCATTTTCGATCCTGGTCAACAAC
TACGGCGGCCGCAGTGTCGCCGAAGTGCGCGGCGTCATGGACCGCATTTGTAATCTGATATTGGAATGA

Upstream 100 bases:

>100_bases
GCTGGGGCGTCGGAGTCGTCATCGGCATCCCGTTGATTGGTACCTTTATGGTGATGATCCTGCTCGTGTTGATCTGGCTC
GAATCCAAGGTGACCGGAAG

Downstream 100 bases:

>100_bases
CCTCGGCCCAGCGTAACGGAACGCGAAATTATAAGGTGCTCTACACACGTTCGCGTCGGTTGGAGGATTCGTCTTGTTGG
AGTGCCGGTCTCCGAGAACG

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein; PBP [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MTAKQRAAVATFLLAALASQCAAAGLPEAIQKLIDSSPAARTAFWGIQIVDLASGKTLYELNPDHYFVPASNAKLFSTAL
ALTRLGPDYTFQTRVFADAPPDSQGRISGDLRLIGGGDPNLSARAIPYRMGPPTGNPLAAIEDLADQLAARGIRIVSGDI
VGDDTWYVWQPYAVGWAIDDPQSDDGPPISALTLSDNALNLSVHPGAAVGEVAELAFSPPLEFYRIENRVRTVAAGGERR
VHFHRIPGSRDAVLWGTIPLRDRGQDMLTSVEDPAEFAAEALRNALEARGITVEGAARSQHLFPNQLADLTQAPPSEAVG
GVELANRSSARLVEDLRVTAKVSQNLHAELLLRAVGRARRNVGSFEAGMTEMKIFLADAGVEPTGYNLLDGSGLSRLDLV
TPATVVRLLRFMYDSPARDNWLSIFPIGGQDGTLSTRFGGTPAAGRVLAKTGSLSHVSALSGYIQRLDGTWLAFSILVNN
YGGRSVAEVRGVMDRICNLILE

Sequences:

>Translated_502_residues
MTAKQRAAVATFLLAALASQCAAAGLPEAIQKLIDSSPAARTAFWGIQIVDLASGKTLYELNPDHYFVPASNAKLFSTAL
ALTRLGPDYTFQTRVFADAPPDSQGRISGDLRLIGGGDPNLSARAIPYRMGPPTGNPLAAIEDLADQLAARGIRIVSGDI
VGDDTWYVWQPYAVGWAIDDPQSDDGPPISALTLSDNALNLSVHPGAAVGEVAELAFSPPLEFYRIENRVRTVAAGGERR
VHFHRIPGSRDAVLWGTIPLRDRGQDMLTSVEDPAEFAAEALRNALEARGITVEGAARSQHLFPNQLADLTQAPPSEAVG
GVELANRSSARLVEDLRVTAKVSQNLHAELLLRAVGRARRNVGSFEAGMTEMKIFLADAGVEPTGYNLLDGSGLSRLDLV
TPATVVRLLRFMYDSPARDNWLSIFPIGGQDGTLSTRFGGTPAAGRVLAKTGSLSHVSALSGYIQRLDGTWLAFSILVNN
YGGRSVAEVRGVMDRICNLILE
>Mature_501_residues
TAKQRAAVATFLLAALASQCAAAGLPEAIQKLIDSSPAARTAFWGIQIVDLASGKTLYELNPDHYFVPASNAKLFSTALA
LTRLGPDYTFQTRVFADAPPDSQGRISGDLRLIGGGDPNLSARAIPYRMGPPTGNPLAAIEDLADQLAARGIRIVSGDIV
GDDTWYVWQPYAVGWAIDDPQSDDGPPISALTLSDNALNLSVHPGAAVGEVAELAFSPPLEFYRIENRVRTVAAGGERRV
HFHRIPGSRDAVLWGTIPLRDRGQDMLTSVEDPAEFAAEALRNALEARGITVEGAARSQHLFPNQLADLTQAPPSEAVGG
VELANRSSARLVEDLRVTAKVSQNLHAELLLRAVGRARRNVGSFEAGMTEMKIFLADAGVEPTGYNLLDGSGLSRLDLVT
PATVVRLLRFMYDSPARDNWLSIFPIGGQDGTLSTRFGGTPAAGRVLAKTGSLSHVSALSGYIQRLDGTWLAFSILVNNY
GGRSVAEVRGVMDRICNLILE

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=479, Percent_Identity=28.1837160751566, Blast_Score=144, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 53631; Mature: 53500

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKQRAAVATFLLAALASQCAAAGLPEAIQKLIDSSPAARTAFWGIQIVDLASGKTLYE
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHEEEECEEEEEECCCCEEEE
LNPDHYFVPASNAKLFSTALALTRLGPDYTFQTRVFADAPPDSQGRISGDLRLIGGGDPN
ECCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCEEEEECCCCC
LSARAIPYRMGPPTGNPLAAIEDLADQLAARGIRIVSGDIVGDDTWYVWQPYAVGWAIDD
CCCEECCEECCCCCCCHHHHHHHHHHHHHHCCEEEEECCEECCCCEEEEECEEEEEEECC
PQSDDGPPISALTLSDNALNLSVHPGAAVGEVAELAFSPPLEFYRIENRVRTVAAGGERR
CCCCCCCCEEEEEECCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHEEECCCCCE
VHFHRIPGSRDAVLWGTIPLRDRGQDMLTSVEDPAEFAAEALRNALEARGITVEGAARSQ
EEEEECCCCCCEEEEEECCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCEEECCCCCC
HLFPNQLADLTQAPPSEAVGGVELANRSSARLVEDLRVTAKVSQNLHAELLLRAVGRARR
CCCHHHHHHHHCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
NVGSFEAGMTEMKIFLADAGVEPTGYNLLDGSGLSRLDLVTPATVVRLLRFMYDSPARDN
CCCCHHCCHHHEEEEEEECCCCCCCCEEECCCCCCCEECCCHHHHHHHHHHHHCCCCCCC
WLSIFPIGGQDGTLSTRFGGTPAAGRVLAKTGSLSHVSALSGYIQRLDGTWLAFSILVNN
CEEEEEECCCCCCEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEHHC
YGGRSVAEVRGVMDRICNLILE
CCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TAKQRAAVATFLLAALASQCAAAGLPEAIQKLIDSSPAARTAFWGIQIVDLASGKTLYE
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHEEEECEEEEEECCCCEEEE
LNPDHYFVPASNAKLFSTALALTRLGPDYTFQTRVFADAPPDSQGRISGDLRLIGGGDPN
ECCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCEEEEECCCCC
LSARAIPYRMGPPTGNPLAAIEDLADQLAARGIRIVSGDIVGDDTWYVWQPYAVGWAIDD
CCCEECCEECCCCCCCHHHHHHHHHHHHHHCCEEEEECCEECCCCEEEEECEEEEEEECC
PQSDDGPPISALTLSDNALNLSVHPGAAVGEVAELAFSPPLEFYRIENRVRTVAAGGERR
CCCCCCCCEEEEEECCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHEEECCCCCE
VHFHRIPGSRDAVLWGTIPLRDRGQDMLTSVEDPAEFAAEALRNALEARGITVEGAARSQ
EEEEECCCCCCEEEEEECCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCEEECCCCCC
HLFPNQLADLTQAPPSEAVGGVELANRSSARLVEDLRVTAKVSQNLHAELLLRAVGRARR
CCCHHHHHHHHCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
NVGSFEAGMTEMKIFLADAGVEPTGYNLLDGSGLSRLDLVTPATVVRLLRFMYDSPARDN
CCCCHHCCHHHEEEEEEECCCCCCCCEEECCCCCCCEECCCHHHHHHHHHHHHCCCCCCC
WLSIFPIGGQDGTLSTRFGGTPAAGRVLAKTGSLSHVSALSGYIQRLDGTWLAFSILVNN
CEEEEEECCCCCCEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEHHC
YGGRSVAEVRGVMDRICNLILE
CCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1554361; 7305936 [H]