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The map label for this gene is gatA [H]

Identifier: 116620010

GI number: 116620010

Start: 1102423

End: 1104219

Strand: Reverse

Name: gatA [H]

Synonym: Acid_0882

Alternate gene names: 116620010

Gene position: 1104219-1102423 (Counterclockwise)

Preceding gene: 116620011

Following gene: 116620009

Centisome position: 11.08

GC content: 68.22

Gene sequence:

>1797_bases
ATGTTCAAGTCGCGCCGGGATTTCCTGAACCGTGCGTCCGCGGCGCTGTTGGCCGCGACCACCGCATGCCGTAAGCCGGC
GCCGCCATCCAGCGCGCTGCCTGCCGGAGCGCCTCCGGCGTTCGGCACGTCGCCGGACGCCGGTCCGCCGGTTTCCGCGG
CGACCTTCGCGGAAGCCGAGAAGCTGGTGCAGTTCGAGCTCACACCCGCGGAGCGCGAGATGGCAGCGGGCAACTGGCGC
AAGAGCCTGGCCCCGGTGTATGAGCGCCGTACCGGGCCGCGCAAGTTTGCGCCGCCCACGCCGGTTGCGCCCGCGACGCG
ATGGGATCCCGTGCTTCCCGGTCAGCAAGCCGGACCCGCGCGCGACCGGTTTGTCCGCACAGGGAACGCGGGGGCGCCTC
TGCCGGCGAGCGAACAGGAGATCGCGTTCGCACCGCTATCGCAGCTCGCTCGCTGGGTGGAAACCCGAAAGATCACCTCG
GAGCGGCTCACGCGCATTTACCTGGACCGGATCCAACGCTTCGACCCCAAGCTGGGGTGCGTGATCACGCTCACGCCGGA
ACTCGCACTCGCGCAGGCGCGGCAGGCCGACCGGGAGATCGCCGCGGGACACTATCGCGGCCCTCTGCACGGCATTCCAT
GGGGCGGAAAGGACCTGCTCGACACGGCCGGCATCGCGACGACTTATGGCGCCGAGCCGTATCGAAACCGCATTCCCCCG
GAGGATGCCGCAGTGGTGAAGCGCCTCCACGACGCCGGCGCGGTGCTGATCGCCAAGTTGAGTCTGGGTGCGCTCGCGCT
GAACGATATCTGGTTCGGCGGGCAGACCATGAATCCCTGGCTGCTGGAGGAAGGCGCCTCGGGATCCAGCGCCGGGCCGG
GCGCAGCTACGGCGGCGGGGCTGGTGGGCTTTTCGATCGGCAGCGAAACCGGGGGCAGCATCGTGAGCCCGAGCATGCGC
TGCGGCGTCACCGGATTGCGGCCGACGTACGGCCGCGTGCCGCGCACAGGAGCCATGACGCTGTGCTGGTCGCTGGATAA
GCTCGGGCCTATGACGCGCAGCGTGGAGGACTCGCTGCTGGTTCTGCAGGCCATCTCCGGCCCTGACGCCGGCGACGTCG
CGAGCGTGCCGAGCCACCTGGATTTCGATGCCTCCGCGCCGGTCGCGGGCCTGCGCGTCGGTTATTTTCCGGGATGGATG
AAGGAGAGTCCCGCGACGGATGTGGATCGCGCCGCGCTCGAGACCACGCGCAAGGCCGGCATGCTGCCGGTGGAAGTGTC
GCTGCCCGATTGGCCGTACAACTCGCTCAGCCTGATTCTCTTCGCCGAGGCCGCCGCGGCATTCGAGGAGCTCACGCTCG
GCGGACATCTCAAGGAGCTCAAGGCGCAGGTCCCGGACGCGTGGCCCAATCTTTTTCGACAGGCGCGATTTCTCTCCGCG
GTGGATTTCGTGCAGGCCGACCGAATGCGCCGGAGCGTGGCGCAGGAAATGGCACGCATTTTTTCGCAGGTGGACCTGCT
GCTGGTTCCCTCGCTTCGGGATGAGATCCTGACGATTACCAACTTCAGCGGGCATCCATCACTGACGCTGCCCGCGGGCT
TCGTGGAAGTCGGCGAGGCACGCAGCGACTGGGCTCCCGACCCGGACCATCCGCTGCCGACATTTTCACCGCCGCGGCGG
GTGCCGCACGGGGTGACGCTGATTGGACGCTTGTTCGATGAAGGCACTCTCGGGCGCGCCGGGCTGGCTCTGGAGCGGGC
GTTCGATATGACCGGGCAGCATCCAGCCGGATTTTAG

Upstream 100 bases:

>100_bases
GACGCTCCCTCACGGTCGCGGCTCTGATGGCTGTCCGGTGCGGAGGGTTTGCAGGCAGCGAGCAAACGAGAGCAGTGTAA
TACAGGACGGGAGGAAAGTA

Downstream 100 bases:

>100_bases
ATCGTTACACTAAAGTCGTGGCGGTGTTCGTAAAGAGCGTTCTGATCGACGCGCCGGTTGACGAGGTCTTCCGCTTCCAC
GAACGCGAGGACGCGCTCGA

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 598; Mature: 598

Protein sequence:

>598_residues
MFKSRRDFLNRASAALLAATTACRKPAPPSSALPAGAPPAFGTSPDAGPPVSAATFAEAEKLVQFELTPAEREMAAGNWR
KSLAPVYERRTGPRKFAPPTPVAPATRWDPVLPGQQAGPARDRFVRTGNAGAPLPASEQEIAFAPLSQLARWVETRKITS
ERLTRIYLDRIQRFDPKLGCVITLTPELALAQARQADREIAAGHYRGPLHGIPWGGKDLLDTAGIATTYGAEPYRNRIPP
EDAAVVKRLHDAGAVLIAKLSLGALALNDIWFGGQTMNPWLLEEGASGSSAGPGAATAAGLVGFSIGSETGGSIVSPSMR
CGVTGLRPTYGRVPRTGAMTLCWSLDKLGPMTRSVEDSLLVLQAISGPDAGDVASVPSHLDFDASAPVAGLRVGYFPGWM
KESPATDVDRAALETTRKAGMLPVEVSLPDWPYNSLSLILFAEAAAAFEELTLGGHLKELKAQVPDAWPNLFRQARFLSA
VDFVQADRMRRSVAQEMARIFSQVDLLLVPSLRDEILTITNFSGHPSLTLPAGFVEVGEARSDWAPDPDHPLPTFSPPRR
VPHGVTLIGRLFDEGTLGRAGLALERAFDMTGQHPAGF

Sequences:

>Translated_598_residues
MFKSRRDFLNRASAALLAATTACRKPAPPSSALPAGAPPAFGTSPDAGPPVSAATFAEAEKLVQFELTPAEREMAAGNWR
KSLAPVYERRTGPRKFAPPTPVAPATRWDPVLPGQQAGPARDRFVRTGNAGAPLPASEQEIAFAPLSQLARWVETRKITS
ERLTRIYLDRIQRFDPKLGCVITLTPELALAQARQADREIAAGHYRGPLHGIPWGGKDLLDTAGIATTYGAEPYRNRIPP
EDAAVVKRLHDAGAVLIAKLSLGALALNDIWFGGQTMNPWLLEEGASGSSAGPGAATAAGLVGFSIGSETGGSIVSPSMR
CGVTGLRPTYGRVPRTGAMTLCWSLDKLGPMTRSVEDSLLVLQAISGPDAGDVASVPSHLDFDASAPVAGLRVGYFPGWM
KESPATDVDRAALETTRKAGMLPVEVSLPDWPYNSLSLILFAEAAAAFEELTLGGHLKELKAQVPDAWPNLFRQARFLSA
VDFVQADRMRRSVAQEMARIFSQVDLLLVPSLRDEILTITNFSGHPSLTLPAGFVEVGEARSDWAPDPDHPLPTFSPPRR
VPHGVTLIGRLFDEGTLGRAGLALERAFDMTGQHPAGF
>Mature_598_residues
MFKSRRDFLNRASAALLAATTACRKPAPPSSALPAGAPPAFGTSPDAGPPVSAATFAEAEKLVQFELTPAEREMAAGNWR
KSLAPVYERRTGPRKFAPPTPVAPATRWDPVLPGQQAGPARDRFVRTGNAGAPLPASEQEIAFAPLSQLARWVETRKITS
ERLTRIYLDRIQRFDPKLGCVITLTPELALAQARQADREIAAGHYRGPLHGIPWGGKDLLDTAGIATTYGAEPYRNRIPP
EDAAVVKRLHDAGAVLIAKLSLGALALNDIWFGGQTMNPWLLEEGASGSSAGPGAATAAGLVGFSIGSETGGSIVSPSMR
CGVTGLRPTYGRVPRTGAMTLCWSLDKLGPMTRSVEDSLLVLQAISGPDAGDVASVPSHLDFDASAPVAGLRVGYFPGWM
KESPATDVDRAALETTRKAGMLPVEVSLPDWPYNSLSLILFAEAAAAFEELTLGGHLKELKAQVPDAWPNLFRQARFLSA
VDFVQADRMRRSVAQEMARIFSQVDLLLVPSLRDEILTITNFSGHPSLTLPAGFVEVGEARSDWAPDPDHPLPTFSPPRR
VPHGVTLIGRLFDEGTLGRAGLALERAFDMTGQHPAGF

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=505, Percent_Identity=27.1287128712871, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI195972892, Length=307, Percent_Identity=26.3843648208469, Blast_Score=100, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17543272, Length=419, Percent_Identity=25.2983293556086, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17537465, Length=310, Percent_Identity=28.0645161290323, Blast_Score=92, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17556264, Length=317, Percent_Identity=28.0757097791798, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17538252, Length=289, Percent_Identity=26.2975778546713, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=399, Percent_Identity=25.8145363408521, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320448, Length=295, Percent_Identity=25.7627118644068, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=439, Percent_Identity=23.6902050113895, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648113, Length=385, Percent_Identity=30.3896103896104, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI21356731, Length=325, Percent_Identity=28, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI161078093, Length=489, Percent_Identity=26.3803680981595, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24644968, Length=240, Percent_Identity=27.9166666666667, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24652985, Length=258, Percent_Identity=27.5193798449612, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922090, Length=258, Percent_Identity=27.5193798449612, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24652981, Length=258, Percent_Identity=27.5193798449612, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24652983, Length=258, Percent_Identity=27.5193798449612, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648435, Length=252, Percent_Identity=29.7619047619048, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648437, Length=252, Percent_Identity=29.7619047619048, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI45550774, Length=263, Percent_Identity=28.8973384030418, Blast_Score=90, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 63856; Mature: 63856

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKSRRDFLNRASAALLAATTACRKPAPPSSALPAGAPPAFGTSPDAGPPVSAATFAEAE
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
KLVQFELTPAEREMAAGNWRKSLAPVYERRTGPRKFAPPTPVAPATRWDPVLPGQQAGPA
HHEEEECCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RDRFVRTGNAGAPLPASEQEIAFAPLSQLARWVETRKITSERLTRIYLDRIQRFDPKLGC
HHHEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
VITLTPELALAQARQADREIAAGHYRGPLHGIPWGGKDLLDTAGIATTYGAEPYRNRIPP
EEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHCCCCC
EDAAVVKRLHDAGAVLIAKLSLGALALNDIWFGGQTMNPWLLEEGASGSSAGPGAATAAG
HHHHHHHHHHHCCCEEEHHHHHCCEEEHHEEECCCCCCCHHEECCCCCCCCCCCHHHHHH
LVGFSIGSETGGSIVSPSMRCGVTGLRPTYGRVPRTGAMTLCWSLDKLGPMTRSVEDSLL
HHHEECCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHEEHHHHHHCCHHHHHHHHEE
VLQAISGPDAGDVASVPSHLDFDASAPVAGLRVGYFPGWMKESPATDVDRAALETTRKAG
EEEECCCCCCCCHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHCC
MLPVEVSLPDWPYNSLSLILFAEAAAAFEELTLGGHLKELKAQVPDAWPNLFRQARFLSA
CCEEEEECCCCCCCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHH
VDFVQADRMRRSVAQEMARIFSQVDLLLVPSLRDEILTITNFSGHPSLTLPAGFVEVGEA
HHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCEEEEECCCCCCCEECCCCHHHHCCC
RSDWAPDPDHPLPTFSPPRRVPHGVTLIGRLFDEGTLGRAGLALERAFDMTGQHPAGF
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MFKSRRDFLNRASAALLAATTACRKPAPPSSALPAGAPPAFGTSPDAGPPVSAATFAEAE
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
KLVQFELTPAEREMAAGNWRKSLAPVYERRTGPRKFAPPTPVAPATRWDPVLPGQQAGPA
HHEEEECCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RDRFVRTGNAGAPLPASEQEIAFAPLSQLARWVETRKITSERLTRIYLDRIQRFDPKLGC
HHHEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
VITLTPELALAQARQADREIAAGHYRGPLHGIPWGGKDLLDTAGIATTYGAEPYRNRIPP
EEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHCCCCC
EDAAVVKRLHDAGAVLIAKLSLGALALNDIWFGGQTMNPWLLEEGASGSSAGPGAATAAG
HHHHHHHHHHHCCCEEEHHHHHCCEEEHHEEECCCCCCCHHEECCCCCCCCCCCHHHHHH
LVGFSIGSETGGSIVSPSMRCGVTGLRPTYGRVPRTGAMTLCWSLDKLGPMTRSVEDSLL
HHHEECCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHEEHHHHHHCCHHHHHHHHEE
VLQAISGPDAGDVASVPSHLDFDASAPVAGLRVGYFPGWMKESPATDVDRAALETTRKAG
EEEECCCCCCCCHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHCC
MLPVEVSLPDWPYNSLSLILFAEAAAAFEELTLGGHLKELKAQVPDAWPNLFRQARFLSA
CCEEEEECCCCCCCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHH
VDFVQADRMRRSVAQEMARIFSQVDLLLVPSLRDEILTITNFSGHPSLTLPAGFVEVGEA
HHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCEEEEECCCCCCCEECCCCHHHHCCC
RSDWAPDPDHPLPTFSPPRRVPHGVTLIGRLFDEGTLGRAGLALERAFDMTGQHPAGF
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA