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The map label for this gene is dapE [H]

Identifier: 116619930

GI number: 116619930

Start: 998366

End: 999736

Strand: Direct

Name: dapE [H]

Synonym: Acid_0802

Alternate gene names: 116619930

Gene position: 998366-999736 (Clockwise)

Preceding gene: 116619927

Following gene: 116619931

Centisome position: 10.02

GC content: 63.82

Gene sequence:

>1371_bases
ATGCGTCTTTTTGCTGCCCTTCTCGTGTTGTTGCCGCTCCACGCGCAAACCTCCGACCAGAGGCTGGCCCGCGAGATTTT
CAAGCAGCTCATCGAAATCAATACCACGGATTCGTCAGGCGACAATACGCGGGCCGCCGAAGCCATGGCCGCGCGTTTCC
GTGCCGCCGGCTTTCCGGCCGCCGACGTCCAGGTGCTGGCGCCGGTCCCGCGCAAAGGCAATCTCGTGGTCCGGCTGCAC
GGCACTGGCACCGGGCGCCCCATTCTCTTCCTCGGGCATCTCGACGTGGTCGAGGCGAGGCGCTCTGACTGGCCGTGGGA
TCCCTTCGAATTCCGCGAGCAGGAAGGCTACTTCTACGGGCGCGGCACCTCCGACATGAAGGGCGACGACGCCACGCTGG
TGGCCGCCTTTCTGCGCATGAAGCGCGAGAATTTCCAGCCTTCACGCGATCTGATTCTCGCGCTCACCTCCGACGAGGAG
GGCGGCCCCGCTAACGGCGTGGACTGGCTGGTCACACAGCACCGCGCTCTGATTGACGCGCAGTTCTGCATCAACACGGA
TGCCGGCGGCGGCCACATCAAGAACGGCAAGCACGTCTATATGGCCATGCAGGCTGCCGAAAAGGTCTTCCTCAGTTTCA
AACTGGAAGTCACCAATAACGGCGGACACAGTTCGCTGCCCGTGAAGGACAACGCGATCTATCATCTGGCCGACGGCCTC
TCGCGGCTATCCAAATTCGATTTTCCGGTCCGTCTCTTCGATGTCACCCGCGCGGCTTTCGAGCGCAGCGCCAATGTTTA
CGGCGGGCAGCTGGGCGCGGACCTCAAGACCATGGTACAGAATCCGGCCGATCCCGCGGCCGTCGCGCGCCTGTCCGCAA
TCCCGTTCTACAACGCGCAGATGCGCACCACCTGTGTCGCCACCATGCTTTCCGGCGGCCACGCTGAGAACGCGCTGCCC
GCGGTCGCGACTGCCGTGGTCAACTGCCGCCTGCTTCCCATCGACAACGCCGCCGACGTGAAGCGTACCCTGCAGCAGGT
CCTCGCCGACCCGAAGATTCAATTGGACGAAATGAAGGAGCCGGTGATCACGCCCTACAAGCCGATCGACCCCGACGTGA
TGGCCGCGGTCGCCGCGAGCACCGCCAGGCTCTGGCCGGGGCTACCCGTGGTGCCCACGATGGACACTGGAGCCAGCGAC
GGCATCTACCTAATCCGCGCCGGCATTCCGACGTACGGCGTCAGCGGCATCTTCGGCGATGAGGACGACGTGCGCGCCCA
CGGCCGTAACGAACGCATCCTGGTGCAGAGCTTCGACCAGGGAGTCGATTTTATTTACGACTTGGCTACTCGCCTGGCGC
GGAGTAACTGA

Upstream 100 bases:

>100_bases
GGTGAATCGGCAGGCGGTCGCCCAGGCTGAAGAAGAGCCCGACGGCTATCAGAACCAGTCCGGCGATGATGAACATCCGC
CCGGGATGCATACTTATAGT

Downstream 100 bases:

>100_bases
CCGGCCGAAAACCACCACCACGGTGCCTGACGGCCAGCCCTGGTGGGCTGAGCGATTGATTCTCCGCAACTTACTAAGTG
GCAAGTAGCTTGCATACTCC

Product: hypothetical protein

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MRLFAALLVLLPLHAQTSDQRLAREIFKQLIEINTTDSSGDNTRAAEAMAARFRAAGFPAADVQVLAPVPRKGNLVVRLH
GTGTGRPILFLGHLDVVEARRSDWPWDPFEFREQEGYFYGRGTSDMKGDDATLVAAFLRMKRENFQPSRDLILALTSDEE
GGPANGVDWLVTQHRALIDAQFCINTDAGGGHIKNGKHVYMAMQAAEKVFLSFKLEVTNNGGHSSLPVKDNAIYHLADGL
SRLSKFDFPVRLFDVTRAAFERSANVYGGQLGADLKTMVQNPADPAAVARLSAIPFYNAQMRTTCVATMLSGGHAENALP
AVATAVVNCRLLPIDNAADVKRTLQQVLADPKIQLDEMKEPVITPYKPIDPDVMAAVAASTARLWPGLPVVPTMDTGASD
GIYLIRAGIPTYGVSGIFGDEDDVRAHGRNERILVQSFDQGVDFIYDLATRLARSN

Sequences:

>Translated_456_residues
MRLFAALLVLLPLHAQTSDQRLAREIFKQLIEINTTDSSGDNTRAAEAMAARFRAAGFPAADVQVLAPVPRKGNLVVRLH
GTGTGRPILFLGHLDVVEARRSDWPWDPFEFREQEGYFYGRGTSDMKGDDATLVAAFLRMKRENFQPSRDLILALTSDEE
GGPANGVDWLVTQHRALIDAQFCINTDAGGGHIKNGKHVYMAMQAAEKVFLSFKLEVTNNGGHSSLPVKDNAIYHLADGL
SRLSKFDFPVRLFDVTRAAFERSANVYGGQLGADLKTMVQNPADPAAVARLSAIPFYNAQMRTTCVATMLSGGHAENALP
AVATAVVNCRLLPIDNAADVKRTLQQVLADPKIQLDEMKEPVITPYKPIDPDVMAAVAASTARLWPGLPVVPTMDTGASD
GIYLIRAGIPTYGVSGIFGDEDDVRAHGRNERILVQSFDQGVDFIYDLATRLARSN
>Mature_456_residues
MRLFAALLVLLPLHAQTSDQRLAREIFKQLIEINTTDSSGDNTRAAEAMAARFRAAGFPAADVQVLAPVPRKGNLVVRLH
GTGTGRPILFLGHLDVVEARRSDWPWDPFEFREQEGYFYGRGTSDMKGDDATLVAAFLRMKRENFQPSRDLILALTSDEE
GGPANGVDWLVTQHRALIDAQFCINTDAGGGHIKNGKHVYMAMQAAEKVFLSFKLEVTNNGGHSSLPVKDNAIYHLADGL
SRLSKFDFPVRLFDVTRAAFERSANVYGGQLGADLKTMVQNPADPAAVARLSAIPFYNAQMRTTCVATMLSGGHAENALP
AVATAVVNCRLLPIDNAADVKRTLQQVLADPKIQLDEMKEPVITPYKPIDPDVMAAVAASTARLWPGLPVVPTMDTGASD
GIYLIRAGIPTYGVSGIFGDEDDVRAHGRNERILVQSFDQGVDFIYDLATRLARSN

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI269846968, Length=450, Percent_Identity=27.1111111111111, Blast_Score=123, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6322289, Length=268, Percent_Identity=26.4925373134328, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24649208, Length=437, Percent_Identity=26.3157894736842, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24649210, Length=438, Percent_Identity=24.6575342465753, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24649212, Length=437, Percent_Identity=22.883295194508, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 49703; Mature: 49703

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00758 ARGE_DAPE_CPG2_1 ; PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFAALLVLLPLHAQTSDQRLAREIFKQLIEINTTDSSGDNTRAAEAMAARFRAAGFPA
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHCCCCC
ADVQVLAPVPRKGNLVVRLHGTGTGRPILFLGHLDVVEARRSDWPWDPFEFREQEGYFYG
CCCEEEECCCCCCCEEEEEECCCCCCCEEEEECCHHHHHHCCCCCCCCHHHHCCCCEEEE
RGTSDMKGDDATLVAAFLRMKRENFQPSRDLILALTSDEEGGPANGVDWLVTQHRALIDA
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHEEE
QFCINTDAGGGHIKNGKHVYMAMQAAEKVFLSFKLEVTNNGGHSSLPVKDNAIYHLADGL
EEEEECCCCCCCCCCCCEEEEEHHHHHEEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHH
SRLSKFDFPVRLFDVTRAAFERSANVYGGQLGADLKTMVQNPADPAAVARLSAIPFYNAQ
HHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCCH
MRTTCVATMLSGGHAENALPAVATAVVNCRLLPIDNAADVKRTLQQVLADPKIQLDEMKE
HHHHHHHHHHCCCCCCCCHHHHHHHHHEEEEEECCCHHHHHHHHHHHHCCCCEEHHHCCC
PVITPYKPIDPDVMAAVAASTARLWPGLPVVPTMDTGASDGIYLIRAGIPTYGVSGIFGD
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCC
EDDVRAHGRNERILVQSFDQGVDFIYDLATRLARSN
CHHHHHCCCCCEEEEEEHHCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRLFAALLVLLPLHAQTSDQRLAREIFKQLIEINTTDSSGDNTRAAEAMAARFRAAGFPA
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHCCCCC
ADVQVLAPVPRKGNLVVRLHGTGTGRPILFLGHLDVVEARRSDWPWDPFEFREQEGYFYG
CCCEEEECCCCCCCEEEEEECCCCCCCEEEEECCHHHHHHCCCCCCCCHHHHCCCCEEEE
RGTSDMKGDDATLVAAFLRMKRENFQPSRDLILALTSDEEGGPANGVDWLVTQHRALIDA
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHEEE
QFCINTDAGGGHIKNGKHVYMAMQAAEKVFLSFKLEVTNNGGHSSLPVKDNAIYHLADGL
EEEEECCCCCCCCCCCCEEEEEHHHHHEEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHH
SRLSKFDFPVRLFDVTRAAFERSANVYGGQLGADLKTMVQNPADPAAVARLSAIPFYNAQ
HHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCCH
MRTTCVATMLSGGHAENALPAVATAVVNCRLLPIDNAADVKRTLQQVLADPKIQLDEMKE
HHHHHHHHHHCCCCCCCCHHHHHHHHHEEEEEECCCHHHHHHHHHHHHCCCCEEHHHCCC
PVITPYKPIDPDVMAAVAASTARLWPGLPVVPTMDTGASDGIYLIRAGIPTYGVSGIFGD
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCC
EDDVRAHGRNERILVQSFDQGVDFIYDLATRLARSN
CHHHHHCCCCCEEEEEEHHCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA