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The map label for this gene is tolB [H]

Identifier: 116619651

GI number: 116619651

Start: 624537

End: 625931

Strand: Direct

Name: tolB [H]

Synonym: Acid_0512

Alternate gene names: 116619651

Gene position: 624537-625931 (Clockwise)

Preceding gene: 116619650

Following gene: 116619652

Centisome position: 6.27

GC content: 58.14

Gene sequence:

>1395_bases
ATGAAGAAACTCATCTGTTTTGCGCTGGCCTCGGCGGGAGCCCTGCTCGTCCTGGCGGCACAGGATGCCCGAATCGTCGT
GGACCGCACCCAGGGGGCCATCCCCGTCATCGCCATCCCGGATCTCCGCGGCTCCGGCGACGCCCAGAAATTCATGGGTG
CCTTCAACGAGACGCTCTATAACGACGTCAACAGCTCCGGTCTTCTGAAAATCGTCCCCAAGACCTCTCTGCCCCTGTTC
GTTCCCCAGCAGCCCTCGGACTTTCAACAGCCTGCTCCGCCGCCCGCCCCTACTCGCGGACGCACTCCGCAGATGACCCA
GGCCGCAAGCGGAGGCGGACGCTGGATGCAGGATTGGTCCAGCCCGCCCCCGCAGGCGAATTATCTGGCCTTCGGCTATA
CGGCCGTGCAAAACGGAGTCTTCGTGCTCCAGGGCTGGCTCTACGATCTGAGTAAGGGTAACCCCGCCGCCGCCCAAGTC
ATCGGCAATCGGTATCTCGGGTCCGTAGACGAGGCCGGCGCCCGCAAAGTCGCCCATGAGTTCGCCGCGGAGATTGTCGG
CCTGTTTGGGGGAAAATCCCTGTTCGGCACTCATATCTATTTCAGTTCTAACCGCTCCGGCCACAAAGAAATCTGGGCTA
TGGATCCGGACGGCAAAAATCAACGCCAGATAACGCGTTTCAATAGCATTTCGACTTACCCCAACGTTTCCCCCGACGGC
ACTAAAATAGCGTTTACTAGCTGGGTGAAGGGGCAACCTGCTATATTTATCTTTTCGGTGGATCCGGTCCGGGATTTGCG
GTTTTATAACCAGACCGCTTCTGTGAACCAGGCAGGTTCTTTTACCCCCGATGGAAAGCAGATTGTCTACGCTTCGTCCG
CGGGGAATGGGATCTGTTGCCGGATCTTCATCGCGAATTTAGACGGAACGGGGTTTCGGCCGATCTCTTCGCCCAGCGCC
ATCGAAGTCGAGCCAAAGGTAAATCCGAAGACCGGCGCCGACATTGTCTTCTCTTCGGGACGCTCCGGTCCGCAACAGAT
TTACCGCATGAATATGGATGGAGCGGACGTGGAACGTTTGACACCCGGAGTTGGTGAAGCTTCCAATCCGTCGTGGCACC
CCGACGGGCAGCACATCGCTTTTGCGTGGACCCAGGGCTATGCCACCGGCGCCTTCAACATATTCACCATGAATGTGGCT
ACTCGGGAATACGTGCAGTTGACGCACGGCGATGGCAAGAACGAAAATCCCAATTGGGCGCCCGACGGCGTGCACATCGT
CTTCGCGAAGACTCGCGGAAATTCCAGCCAGATTTATACCATGCTGGCCAATGGAACCCAGATACAGCAACTAACTACCG
CGGGCCAGAATGAGAGGCCGGTATGGGGCAGGTAA

Upstream 100 bases:

>100_bases
TATGGACGCGTCGCCGTTCCCCCAAATGCCGCCTCAGTTCCCCAAGAGCGAGGCGGAAATCGAATTCGTGTTTCAACTGA
AACGCTAGAGGATCAAAGGC

Downstream 100 bases:

>100_bases
CGCAAGTTTAGGAGGCGATGTTCATGTTCAGTAAACGGAAGTTGTCTACCATCGGCCTGGCGCTCGCGCTGGCGATGTTT
GCGGTTGGATGCAAGAAGAA

Product: WD40 domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MKKLICFALASAGALLVLAAQDARIVVDRTQGAIPVIAIPDLRGSGDAQKFMGAFNETLYNDVNSSGLLKIVPKTSLPLF
VPQQPSDFQQPAPPPAPTRGRTPQMTQAASGGGRWMQDWSSPPPQANYLAFGYTAVQNGVFVLQGWLYDLSKGNPAAAQV
IGNRYLGSVDEAGARKVAHEFAAEIVGLFGGKSLFGTHIYFSSNRSGHKEIWAMDPDGKNQRQITRFNSISTYPNVSPDG
TKIAFTSWVKGQPAIFIFSVDPVRDLRFYNQTASVNQAGSFTPDGKQIVYASSAGNGICCRIFIANLDGTGFRPISSPSA
IEVEPKVNPKTGADIVFSSGRSGPQQIYRMNMDGADVERLTPGVGEASNPSWHPDGQHIAFAWTQGYATGAFNIFTMNVA
TREYVQLTHGDGKNENPNWAPDGVHIVFAKTRGNSSQIYTMLANGTQIQQLTTAGQNERPVWGR

Sequences:

>Translated_464_residues
MKKLICFALASAGALLVLAAQDARIVVDRTQGAIPVIAIPDLRGSGDAQKFMGAFNETLYNDVNSSGLLKIVPKTSLPLF
VPQQPSDFQQPAPPPAPTRGRTPQMTQAASGGGRWMQDWSSPPPQANYLAFGYTAVQNGVFVLQGWLYDLSKGNPAAAQV
IGNRYLGSVDEAGARKVAHEFAAEIVGLFGGKSLFGTHIYFSSNRSGHKEIWAMDPDGKNQRQITRFNSISTYPNVSPDG
TKIAFTSWVKGQPAIFIFSVDPVRDLRFYNQTASVNQAGSFTPDGKQIVYASSAGNGICCRIFIANLDGTGFRPISSPSA
IEVEPKVNPKTGADIVFSSGRSGPQQIYRMNMDGADVERLTPGVGEASNPSWHPDGQHIAFAWTQGYATGAFNIFTMNVA
TREYVQLTHGDGKNENPNWAPDGVHIVFAKTRGNSSQIYTMLANGTQIQQLTTAGQNERPVWGR
>Mature_464_residues
MKKLICFALASAGALLVLAAQDARIVVDRTQGAIPVIAIPDLRGSGDAQKFMGAFNETLYNDVNSSGLLKIVPKTSLPLF
VPQQPSDFQQPAPPPAPTRGRTPQMTQAASGGGRWMQDWSSPPPQANYLAFGYTAVQNGVFVLQGWLYDLSKGNPAAAQV
IGNRYLGSVDEAGARKVAHEFAAEIVGLFGGKSLFGTHIYFSSNRSGHKEIWAMDPDGKNQRQITRFNSISTYPNVSPDG
TKIAFTSWVKGQPAIFIFSVDPVRDLRFYNQTASVNQAGSFTPDGKQIVYASSAGNGICCRIFIANLDGTGFRPISSPSA
IEVEPKVNPKTGADIVFSSGRSGPQQIYRMNMDGADVERLTPGVGEASNPSWHPDGQHIAFAWTQGYATGAFNIFTMNVA
TREYVQLTHGDGKNENPNWAPDGVHIVFAKTRGNSSQIYTMLANGTQIQQLTTAGQNERPVWGR

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=301, Percent_Identity=24.9169435215947, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 50081; Mature: 50081

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLICFALASAGALLVLAAQDARIVVDRTQGAIPVIAIPDLRGSGDAQKFMGAFNETLY
CCCEEEEEECCCCEEEEEEECCCEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHH
NDVNSSGLLKIVPKTSLPLFVPQQPSDFQQPAPPPAPTRGRTPQMTQAASGGGRWMQDWS
HCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHCC
SPPPQANYLAFGYTAVQNGVFVLQGWLYDLSKGNPAAAQVIGNRYLGSVDEAGARKVAHE
CCCCCCCEEEEEEHHHHCCEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHH
FAAEIVGLFGGKSLFGTHIYFSSNRSGHKEIWAMDPDGKNQRQITRFNSISTYPNVSPDG
HHHHHHHHCCCCCEEEEEEEEECCCCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCC
TKIAFTSWVKGQPAIFIFSVDPVRDLRFYNQTASVNQAGSFTPDGKQIVYASSAGNGICC
CEEEEEEECCCCCEEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCEEE
RIFIANLDGTGFRPISSPSAIEVEPKVNPKTGADIVFSSGRSGPQQIYRMNMDGADVERL
EEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEECCCCCCHHHEEECCCCCCHHHC
TPGVGEASNPSWHPDGQHIAFAWTQGYATGAFNIFTMNVATREYVQLTHGDGKNENPNWA
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEEEECCEEEEEEECCCCCCCCCCCC
PDGVHIVFAKTRGNSSQIYTMLANGTQIQQLTTAGQNERPVWGR
CCCEEEEEEEECCCCCEEEEEEECCCEEEHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKKLICFALASAGALLVLAAQDARIVVDRTQGAIPVIAIPDLRGSGDAQKFMGAFNETLY
CCCEEEEEECCCCEEEEEEECCCEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHH
NDVNSSGLLKIVPKTSLPLFVPQQPSDFQQPAPPPAPTRGRTPQMTQAASGGGRWMQDWS
HCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHCC
SPPPQANYLAFGYTAVQNGVFVLQGWLYDLSKGNPAAAQVIGNRYLGSVDEAGARKVAHE
CCCCCCCEEEEEEHHHHCCEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHH
FAAEIVGLFGGKSLFGTHIYFSSNRSGHKEIWAMDPDGKNQRQITRFNSISTYPNVSPDG
HHHHHHHHCCCCCEEEEEEEEECCCCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCC
TKIAFTSWVKGQPAIFIFSVDPVRDLRFYNQTASVNQAGSFTPDGKQIVYASSAGNGICC
CEEEEEEECCCCCEEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCEEE
RIFIANLDGTGFRPISSPSAIEVEPKVNPKTGADIVFSSGRSGPQQIYRMNMDGADVERL
EEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEECCCCCCHHHEEECCCCCCHHHC
TPGVGEASNPSWHPDGQHIAFAWTQGYATGAFNIFTMNVATREYVQLTHGDGKNENPNWA
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEEEECCEEEEEEECCCCCCCCCCCC
PDGVHIVFAKTRGNSSQIYTMLANGTQIQQLTTAGQNERPVWGR
CCCEEEEEEEECCCCCEEEEEEECCCEEEHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA