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The map label for this gene is ybiK [C]

Identifier: 116619627

GI number: 116619627

Start: 598094

End: 599296

Strand: Direct

Name: ybiK [C]

Synonym: Acid_0488

Alternate gene names: 116619627

Gene position: 598094-599296 (Clockwise)

Preceding gene: 116619618

Following gene: 116619628

Centisome position: 6.0

GC content: 60.27

Gene sequence:

>1203_bases
TTGCGTTTTCCGTTGCCGTTACGGAAAGCCCGGCAAGCGGCCGTTACGCTGCTGTTGCTGGCGGAAGGAGAAATCTGCAT
GAAGGACATGAGTTTCAGCAGGCGGGCGATAATGATGGGTGCGGCCGCGCTTCCCATGGCGCGGAAACTCGTCGCCCAAG
CTCGTCCGAAGAGTGTCGTGATTTCGACTGCCAACGCGTTCAATGGCGGCATCAATTGCTGCGCGAAGGCCATGGAAGCG
ATCAAATCCGGCACGGATACCCTGGATGCGGTGATCGCCGGCGTCAACATTCCGGAACTGGATCCGCGGGAAACCAGCGT
TGGGTATGGCGGACTGCCAAATGAAGATTGCGTGGTGGAACTCGACGCCAGCGTGATTCACGGCCCGTCGAGGCGCGGTG
GTGCCGTGGGCGCGCTCCGCGGCATCAAGACTCCCTCGAAGATCGCGCAACTGGTGATGGCCGAGACCGATCACATGATG
CTGGTGGGTGAAGGCGCACTGCGCTTCGCCAAGGCGTGGGGCTACGCGGAAGAAGATCTGCTCACCGACCGCTCGCGCCT
TGCCTGGCGCATGTGGAAACGCGAGATGCGCGATCGCGGCGGACATACCAACTGGGAGAGCAGCACCGATGCGCCTCCCG
CGAAGAAAATGGCCGAGCTGAAGAAGGCCTTCCCGGGTGCGGATGAAGAGTCGCTGGCTTGGGCCTACGAGATTGCATTA
AATCCGCCGCCGCACGGCACCATCAACTGCCTGGCGCTGAATGAAAAGGGCGAGATGTCCGCGGTGACGACTACCAGCGG
CGCGGCCTTCAAGATCCCCGGCCGGCTGGGCGATTCGCCGATCATCGGCGGAGGCATGTGGCTGGACCAGGATATCGGGG
CCGCGGGTTCGACCGGACGCGGCGAAGAAAACTTGAGAGTTTGTGGGGCGCATACGATTGTCGAGAACATGCGGCAAGGT
ATGCGTCCGAAGGATGCGATCCTCGATGCGTTCAAAAGGGTGGCTCGTAATTTTGATAATGATGCGGGCCGGCTTGCGCA
GATCGATCTGAATTTTTACGCACTGAATAAGGATGGGGAGTACGCAGGCGGTTCGCTGTGGGACAGACGTAGCAAGGGTG
CACCTGCCGCCCAGTTCGCAGTCTGCACCGGCGACAGCAATAGCAGGCACGAAAACTGCATATTTTTAATGCAGAGAAGC
TGA

Upstream 100 bases:

>100_bases
ATCACACGGTGATCACAACTGCGCCAGCCGCGACGACGTAAGATAGCGGACTATTTCCTCCCGGTTTCCCTATGACATAA
TGTGACTTCTTTCCCAGTCC

Downstream 100 bases:

>100_bases
GTTTGTAGAAGAATTCCTCGCGTGCGGAGGTCACCAAGTGTTGTGGCGTGCGCAAGCGATCCGATAAAATGAAACAAAAC
CGTTGACGCCGTCATCTCTA

Product: glycosylasparaginase

Products: NA

Alternate protein names: Aspartylglucosaminidase; AGA; Glycosylasparaginase; N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MRFPLPLRKARQAAVTLLLLAEGEICMKDMSFSRRAIMMGAAALPMARKLVAQARPKSVVISTANAFNGGINCCAKAMEA
IKSGTDTLDAVIAGVNIPELDPRETSVGYGGLPNEDCVVELDASVIHGPSRRGGAVGALRGIKTPSKIAQLVMAETDHMM
LVGEGALRFAKAWGYAEEDLLTDRSRLAWRMWKREMRDRGGHTNWESSTDAPPAKKMAELKKAFPGADEESLAWAYEIAL
NPPPHGTINCLALNEKGEMSAVTTTSGAAFKIPGRLGDSPIIGGGMWLDQDIGAAGSTGRGEENLRVCGAHTIVENMRQG
MRPKDAILDAFKRVARNFDNDAGRLAQIDLNFYALNKDGEYAGGSLWDRRSKGAPAAQFAVCTGDSNSRHENCIFLMQRS

Sequences:

>Translated_400_residues
MRFPLPLRKARQAAVTLLLLAEGEICMKDMSFSRRAIMMGAAALPMARKLVAQARPKSVVISTANAFNGGINCCAKAMEA
IKSGTDTLDAVIAGVNIPELDPRETSVGYGGLPNEDCVVELDASVIHGPSRRGGAVGALRGIKTPSKIAQLVMAETDHMM
LVGEGALRFAKAWGYAEEDLLTDRSRLAWRMWKREMRDRGGHTNWESSTDAPPAKKMAELKKAFPGADEESLAWAYEIAL
NPPPHGTINCLALNEKGEMSAVTTTSGAAFKIPGRLGDSPIIGGGMWLDQDIGAAGSTGRGEENLRVCGAHTIVENMRQG
MRPKDAILDAFKRVARNFDNDAGRLAQIDLNFYALNKDGEYAGGSLWDRRSKGAPAAQFAVCTGDSNSRHENCIFLMQRS
>Mature_400_residues
MRFPLPLRKARQAAVTLLLLAEGEICMKDMSFSRRAIMMGAAALPMARKLVAQARPKSVVISTANAFNGGINCCAKAMEA
IKSGTDTLDAVIAGVNIPELDPRETSVGYGGLPNEDCVVELDASVIHGPSRRGGAVGALRGIKTPSKIAQLVMAETDHMM
LVGEGALRFAKAWGYAEEDLLTDRSRLAWRMWKREMRDRGGHTNWESSTDAPPAKKMAELKKAFPGADEESLAWAYEIAL
NPPPHGTINCLALNEKGEMSAVTTTSGAAFKIPGRLGDSPIIGGGMWLDQDIGAAGSTGRGEENLRVCGAHTIVENMRQG
MRPKDAILDAFKRVARNFDNDAGRLAQIDLNFYALNKDGEYAGGSLWDRRSKGAPAAQFAVCTGDSNSRHENCIFLMQRS

Specific function: Cleaves the GlcNAc-Asn bond which joins oligosaccharides to the peptide of asparagine-linked glycoproteins. Requires that the glycosylated asparagine moiety is not substituted on its N- (R1) and C- (R2) terminus [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI285002251, Length=267, Percent_Identity=34.8314606741573, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI145275202, Length=249, Percent_Identity=32.5301204819277, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI145275200, Length=249, Percent_Identity=32.5301204819277, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI285002253, Length=267, Percent_Identity=32.5842696629214, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI98991769, Length=231, Percent_Identity=29.8701298701299, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17569239, Length=266, Percent_Identity=31.9548872180451, Blast_Score=120, Evalue=9e-28,
Organism=Drosophila melanogaster, GI221330102, Length=266, Percent_Identity=34.5864661654135, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24652135, Length=266, Percent_Identity=34.5864661654135, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24655553, Length=279, Percent_Identity=30.1075268817204, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24657358, Length=225, Percent_Identity=30.2222222222222, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.5.1.26 [H]

Molecular weight: Translated: 43108; Mature: 43108

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFPLPLRKARQAAVTLLLLAEGEICMKDMSFSRRAIMMGAAALPMARKLVAQARPKSVV
CCCCCCHHHHHHHHEEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
ISTANAFNGGINCCAKAMEAIKSGTDTLDAVIAGVNIPELDPRETSVGYGGLPNEDCVVE
EEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEE
LDASVIHGPSRRGGAVGALRGIKTPSKIAQLVMAETDHMMLVGEGALRFAKAWGYAEEDL
ECHHHCCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCHHHH
LTDRSRLAWRMWKREMRDRGGHTNWESSTDAPPAKKMAELKKAFPGADEESLAWAYEIAL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEEE
NPPPHGTINCLALNEKGEMSAVTTTSGAAFKIPGRLGDSPIIGGGMWLDQDIGAAGSTGR
CCCCCCCEEEEEECCCCCEEEEEECCCCEEEECCCCCCCCEECCCEEECCCCCCCCCCCC
GEENLRVCGAHTIVENMRQGMRPKDAILDAFKRVARNFDNDAGRLAQIDLNFYALNKDGE
CCCCEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCC
YAGGSLWDRRSKGAPAAQFAVCTGDSNSRHENCIFLMQRS
CCCCCHHHCCCCCCCCEEEEEEECCCCCCCCCEEEEEECC
>Mature Secondary Structure
MRFPLPLRKARQAAVTLLLLAEGEICMKDMSFSRRAIMMGAAALPMARKLVAQARPKSVV
CCCCCCHHHHHHHHEEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
ISTANAFNGGINCCAKAMEAIKSGTDTLDAVIAGVNIPELDPRETSVGYGGLPNEDCVVE
EEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEE
LDASVIHGPSRRGGAVGALRGIKTPSKIAQLVMAETDHMMLVGEGALRFAKAWGYAEEDL
ECHHHCCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCHHHH
LTDRSRLAWRMWKREMRDRGGHTNWESSTDAPPAKKMAELKKAFPGADEESLAWAYEIAL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEEE
NPPPHGTINCLALNEKGEMSAVTTTSGAAFKIPGRLGDSPIIGGGMWLDQDIGAAGSTGR
CCCCCCCEEEEEECCCCCEEEEEECCCCEEEECCCCCCCCEECCCEEECCCCCCCCCCCC
GEENLRVCGAHTIVENMRQGMRPKDAILDAFKRVARNFDNDAGRLAQIDLNFYALNKDGE
CCCCEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCC
YAGGSLWDRRSKGAPAAQFAVCTGDSNSRHENCIFLMQRS
CCCCCHHHCCCCCCCCEEEEEEECCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7840643; 8250923; 9541410 [H]