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The map label for this gene is pcaK [H]

Identifier: 116619395

GI number: 116619395

Start: 338034

End: 339401

Strand: Direct

Name: pcaK [H]

Synonym: Acid_0254

Alternate gene names: 116619395

Gene position: 338034-339401 (Clockwise)

Preceding gene: 116619394

Following gene: 116619398

Centisome position: 3.39

GC content: 66.01

Gene sequence:

>1368_bases
GTGAGCGCCCCGGTCGAGATCGGCTCGCTGCTGGATCGCGGCCGATGGACGCCGTACCAGAGACTACTCACCGCGCTCGC
CGCGCTGGCAGTTATCTTCGATGGCTTCGACATTCAGATTCTCGGCTTCGCGATTCCGTCGCTGATGAAGGAGTGGGGCG
TCGCGCGGCCGGCATTCGGTCCGGTGTTGGCTCTCGGGCTGGCAGGGATGGCGGCCGGGAGTCCGTTTGCCGGCTACTGG
GGCGATCGATGGGGACGCCGTCCCGCGCTGATCGGCTGCGTCGCTCTCTTCGGCCTGGCGACGGTCGCGACGTCGTCTGT
CCATTCGGTGTTCGCGCTCGCTGTTTTGCGTTTTGTCACTGGATTGGGCGCCGGCGGAGCGGTGCCGAACGCCACCGCGT
TCGCGGCTGAGTTCGCGCCCTTGCGGCAGCGCGCCGTGGCGGTGAAGCTGACCATCGTTTGCGTTCCCCTGGGAGGAATG
CTGGGCGGTCTGATCGCGGCGCAGGTGCTTCCCGCGCTGGGCTGGCGCGCGCTGTATCAGATTGGTGGGGCGCTGCCGCT
GGCGTTCGCGGCCATACTTTGGACGGTGCTCCCGGAGTCGCCCAGCTTTCTGGCGCAACGTCCGCAAAGATGGCCGGCGC
TGGAGCAACTTCTGCGGCGGATGAGCCGCGCCGTCCCGGCCGGCAGCAGCTTCGAAGATCAGCGTGAAAGCGGGTCGGTC
CGCCGCGCACCCATCCGCGAACTGCTCGGGTCCCGGCACTTGCGCGATACCGTTGGCTTGTGGCTCGCTTTCTTTTTCTG
CCTGGGTGCCATTTACCTGGTGTTTGGCTGGCTGCCTGCCATGTTGACCTCGCAAGGGATGGACGTTGCCGGCGCCAGTC
GAGGTCTCGCTCTCTACAATTTTGGCGGCGTGCTGGGTGTCTTCATCTGGGCCGTGCTGATGACGCACCTTGGTTCACGC
CGTCCGCTGCTTTCAGGCGCACTCGCTGCGGCCGGTACCGCGATCGCTATCCTGCTAGTGCCGGTGCAATCGCAGGGCGG
GCGCGAGTTGATGTTCGCTGGGTTGGCCCTGAACGGACTGGTGTCGAACGCTGTGCAGACGTCGATGTATTCACTGGCTG
CGCACGTATATCCCACCGCTGTGCGCGCCTCTGGTGTCGCTTATGCGGCCGCCATCGGGCGCGTGGGAGGCATCTTCAGT
TCCATCTTCGGCGCCGCGATCATCAGCGCGGGAGCGGGCGCTTACTGGGGAACGCTCGCCGCGTCGATGGCCTGCGCGTT
CGCAGGCCTCGCCTGGGTGCGCAGTCACTACCCTGCGTTGGCAAAGGAGAATGCCGAGGCGGTGCGCCCGGATCAAGAGC
GTTTATAA

Upstream 100 bases:

>100_bases
GCGGCTTCGCCAGCACCGAAGAAGGCAACTCCCTCACCGAAGACGAGCAGTGGGCGAAGTTGGCGCGCATCGTCGAAGTG
GCCGGGAAGGTGTGGGGAGC

Downstream 100 bases:

>100_bases
GACTGCAGGCCCGGCTTGATGCTCTTCTCGTCAAGGAACTGCTTCATCCCCTCCGCGCGCGCATCTTCGGGATCGCGATG
CAAAGCCTGATCGAGCTTCG

Product: major facilitator transporter

Products: Proton [Cytoplasm]; 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MSAPVEIGSLLDRGRWTPYQRLLTALAALAVIFDGFDIQILGFAIPSLMKEWGVARPAFGPVLALGLAGMAAGSPFAGYW
GDRWGRRPALIGCVALFGLATVATSSVHSVFALAVLRFVTGLGAGGAVPNATAFAAEFAPLRQRAVAVKLTIVCVPLGGM
LGGLIAAQVLPALGWRALYQIGGALPLAFAAILWTVLPESPSFLAQRPQRWPALEQLLRRMSRAVPAGSSFEDQRESGSV
RRAPIRELLGSRHLRDTVGLWLAFFFCLGAIYLVFGWLPAMLTSQGMDVAGASRGLALYNFGGVLGVFIWAVLMTHLGSR
RPLLSGALAAAGTAIAILLVPVQSQGGRELMFAGLALNGLVSNAVQTSMYSLAAHVYPTAVRASGVAYAAAIGRVGGIFS
SIFGAAIISAGAGAYWGTLAASMACAFAGLAWVRSHYPALAKENAEAVRPDQERL

Sequences:

>Translated_455_residues
MSAPVEIGSLLDRGRWTPYQRLLTALAALAVIFDGFDIQILGFAIPSLMKEWGVARPAFGPVLALGLAGMAAGSPFAGYW
GDRWGRRPALIGCVALFGLATVATSSVHSVFALAVLRFVTGLGAGGAVPNATAFAAEFAPLRQRAVAVKLTIVCVPLGGM
LGGLIAAQVLPALGWRALYQIGGALPLAFAAILWTVLPESPSFLAQRPQRWPALEQLLRRMSRAVPAGSSFEDQRESGSV
RRAPIRELLGSRHLRDTVGLWLAFFFCLGAIYLVFGWLPAMLTSQGMDVAGASRGLALYNFGGVLGVFIWAVLMTHLGSR
RPLLSGALAAAGTAIAILLVPVQSQGGRELMFAGLALNGLVSNAVQTSMYSLAAHVYPTAVRASGVAYAAAIGRVGGIFS
SIFGAAIISAGAGAYWGTLAASMACAFAGLAWVRSHYPALAKENAEAVRPDQERL
>Mature_454_residues
SAPVEIGSLLDRGRWTPYQRLLTALAALAVIFDGFDIQILGFAIPSLMKEWGVARPAFGPVLALGLAGMAAGSPFAGYWG
DRWGRRPALIGCVALFGLATVATSSVHSVFALAVLRFVTGLGAGGAVPNATAFAAEFAPLRQRAVAVKLTIVCVPLGGML
GGLIAAQVLPALGWRALYQIGGALPLAFAAILWTVLPESPSFLAQRPQRWPALEQLLRRMSRAVPAGSSFEDQRESGSVR
RAPIRELLGSRHLRDTVGLWLAFFFCLGAIYLVFGWLPAMLTSQGMDVAGASRGLALYNFGGVLGVFIWAVLMTHLGSRR
PLLSGALAAAGTAIAILLVPVQSQGGRELMFAGLALNGLVSNAVQTSMYSLAAHVYPTAVRASGVAYAAAIGRVGGIFSS
IFGAAIISAGAGAYWGTLAASMACAFAGLAWVRSHYPALAKENAEAVRPDQERL

Specific function: Could Be A Transporter For 3-Phenylpropionate (Hydrocinnamic Acid). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Homo sapiens, GI24308167, Length=446, Percent_Identity=26.6816143497758, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI7662270, Length=231, Percent_Identity=25.1082251082251, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI213021148, Length=410, Percent_Identity=23.6585365853659, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI87081723, Length=405, Percent_Identity=29.8765432098765, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1786229, Length=375, Percent_Identity=26.6666666666667, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI87082404, Length=393, Percent_Identity=23.1552162849873, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87082231, Length=423, Percent_Identity=22.9314420803783, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI87082159, Length=410, Percent_Identity=25.609756097561, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788068, Length=340, Percent_Identity=23.8235294117647, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788074, Length=349, Percent_Identity=24.9283667621776, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789207, Length=234, Percent_Identity=26.9230769230769, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI32564663, Length=411, Percent_Identity=26.5206812652068, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19922874, Length=433, Percent_Identity=26.7898383371824, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004746
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47590; Mature: 47459

Theoretical pI: Translated: 10.59; Mature: 10.59

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPVEIGSLLDRGRWTPYQRLLTALAALAVIFDGFDIQILGFAIPSLMKEWGVARPAFG
CCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHH
PVLALGLAGMAAGSPFAGYWGDRWGRRPALIGCVALFGLATVATSSVHSVFALAVLRFVT
HHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLGAGGAVPNATAFAAEFAPLRQRAVAVKLTIVCVPLGGMLGGLIAAQVLPALGWRALYQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
IGGALPLAFAAILWTVLPESPSFLAQRPQRWPALEQLLRRMSRAVPAGSSFEDQRESGSV
HCCHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCCC
RRAPIRELLGSRHLRDTVGLWLAFFFCLGAIYLVFGWLPAMLTSQGMDVAGASRGLALYN
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHH
FGGVLGVFIWAVLMTHLGSRRPLLSGALAAAGTAIAILLVPVQSQGGRELMFAGLALNGL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEEEEEECCCCCCCCHHHHHHHHHHH
VSNAVQTSMYSLAAHVYPTAVRASGVAYAAAIGRVGGIFSSIFGAAIISAGAGAYWGTLA
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ASMACAFAGLAWVRSHYPALAKENAEAVRPDQERL
HHHHHHHHHHHHHHHHCCHHHHCCCHHCCCCCCCC
>Mature Secondary Structure 
SAPVEIGSLLDRGRWTPYQRLLTALAALAVIFDGFDIQILGFAIPSLMKEWGVARPAFG
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHH
PVLALGLAGMAAGSPFAGYWGDRWGRRPALIGCVALFGLATVATSSVHSVFALAVLRFVT
HHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLGAGGAVPNATAFAAEFAPLRQRAVAVKLTIVCVPLGGMLGGLIAAQVLPALGWRALYQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
IGGALPLAFAAILWTVLPESPSFLAQRPQRWPALEQLLRRMSRAVPAGSSFEDQRESGSV
HCCHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCCC
RRAPIRELLGSRHLRDTVGLWLAFFFCLGAIYLVFGWLPAMLTSQGMDVAGASRGLALYN
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHH
FGGVLGVFIWAVLMTHLGSRRPLLSGALAAAGTAIAILLVPVQSQGGRELMFAGLALNGL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEEEEEECCCCCCCCHHHHHHHHHHH
VSNAVQTSMYSLAAHVYPTAVRASGVAYAAAIGRVGGIFSSIFGAAIISAGAGAYWGTLA
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ASMACAFAGLAWVRSHYPALAKENAEAVRPDQERL
HHHHHHHHHHHHHHHHCCHHHHCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 3-(3-hydroxyphenyl)propionate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 3-(3-hydroxyphenyl)propionate [Periplasm] = Proton [Cytoplasm] + 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8063101 [H]