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The map label for this gene is pcaF [H]

Identifier: 116619392

GI number: 116619392

Start: 334542

End: 335735

Strand: Direct

Name: pcaF [H]

Synonym: Acid_0251

Alternate gene names: 116619392

Gene position: 334542-335735 (Clockwise)

Preceding gene: 116619391

Following gene: 116619393

Centisome position: 3.36

GC content: 68.43

Gene sequence:

>1194_bases
ATGCGGCAAGCCTTCCTCTGCGACGCGGTTCGCACGCCCTTCGGGCGTTACGGAGGCGCACTGGCGGGAGTCCGCACGGA
TGACCTGGCGGCCATCCCTCTGCGAGCGTTGCTGGGGCGCAATCCGCAACTCGACCCGGGCGCACTCGATGACGTCGTCT
ACGGCTGCGTGAATCAGGCAGGTGAGGACAATCGCAATGTCGCCCGCATGGCGCTGCTGCTGGCGGGAATCCCGGAGAGC
GTGCCGGGAACGACGGTGAATCGATTGTGCGGATCGAGTCTGGAAGCGGCCGCGGTGGCGGCCCGCGCCATCCGCTGCGG
AGAAATCGCGCTGGCGATCGCCGGCGGTGTGGAAAGCATGTCTCGCTCACCGCTGGTGCTCTCGAAGCCCGACGCCGCGT
GGACGCGCTCGCAGAAGATCGAAGACTCGACCCTCGGGTGGCGCTTCGTCAACCCCCTCATGAAAGAGCGATACGGCGTC
GACTCGATGGCGGAAACGGCCGAAAACGTCGCCACCGAATGCGGCATCCTGCGCGCCGACCAGGACGCCTTTGCATTTCG
AAGCCAACAGCGCGCAGGCGCGGCGATGACGGCGAGCCGGCTGGCGGAAGAGATCGTAGCCGTCGAGATTTCGAAGAAGC
CCGCCGTGTCGACTGACGAACATCCGCGGCCGGACACCACCCTGGAAGCGCTCACCGCGCTACGTCCGATCGTCAGGCCC
GGCGGTACGGTGACTGCGGGGAACGCGTCGGGCATCAACGACGGCGCAGGCGCCCTGCTGCTGGCCGGCGAGAGCGCCGC
GGCGCAATACGGCCTGACTCCGAAGGCGCGCATTGTGGCGACTGCCGTGGCTGGAGTCGCGCCGCGCGCGATGGGCCTGG
GACCAGTGCCCGCCGTGCGGAAGGTGCTCGCCCTCACCGGTCTCACCCTCGGCCAAATCGACCTGATCGAATTGAACGAA
GCATTCGCCGCGCAGGCGCTGGCGGTGCTGCGCGCACTGGGACTTGCCGACGATGCGGCGCACGTCAATCCCGATGGCGG
CGCAATCGCCCTCGGCCATCCGCTCGGGGCGAGCGGCGCGAGACTGCTGGGTGCGGCCGTGCGGCAACTGCAATGCGGCG
GGGGCAGGTACGCCCTGTGCACCATGTGCATCGGTGTGGGGCAGGGGATTGCGACCATCGTGGAGCGCGTCTGA

Upstream 100 bases:

>100_bases
CCGCGAAGGCGCGGACCGGCTCTCGATCGCGCTTCCATGAGTCGTCGCAACTTGACAAGGCTCGCGCGTTTATCGTACTA
ATAGCCGATAAGCGCACATC

Downstream 100 bases:

>100_bases
AGACCATGCGCACGCAGGTGGGCATCGTCGGCGCCGGACCGGCCGGCTTACTGCTGGCGCACCTGCTTCACCGGCAGGGC
ATCGAGTCCGTTGTGATCGA

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MRQAFLCDAVRTPFGRYGGALAGVRTDDLAAIPLRALLGRNPQLDPGALDDVVYGCVNQAGEDNRNVARMALLLAGIPES
VPGTTVNRLCGSSLEAAAVAARAIRCGEIALAIAGGVESMSRSPLVLSKPDAAWTRSQKIEDSTLGWRFVNPLMKERYGV
DSMAETAENVATECGILRADQDAFAFRSQQRAGAAMTASRLAEEIVAVEISKKPAVSTDEHPRPDTTLEALTALRPIVRP
GGTVTAGNASGINDGAGALLLAGESAAAQYGLTPKARIVATAVAGVAPRAMGLGPVPAVRKVLALTGLTLGQIDLIELNE
AFAAQALAVLRALGLADDAAHVNPDGGAIALGHPLGASGARLLGAAVRQLQCGGGRYALCTMCIGVGQGIATIVERV

Sequences:

>Translated_397_residues
MRQAFLCDAVRTPFGRYGGALAGVRTDDLAAIPLRALLGRNPQLDPGALDDVVYGCVNQAGEDNRNVARMALLLAGIPES
VPGTTVNRLCGSSLEAAAVAARAIRCGEIALAIAGGVESMSRSPLVLSKPDAAWTRSQKIEDSTLGWRFVNPLMKERYGV
DSMAETAENVATECGILRADQDAFAFRSQQRAGAAMTASRLAEEIVAVEISKKPAVSTDEHPRPDTTLEALTALRPIVRP
GGTVTAGNASGINDGAGALLLAGESAAAQYGLTPKARIVATAVAGVAPRAMGLGPVPAVRKVLALTGLTLGQIDLIELNE
AFAAQALAVLRALGLADDAAHVNPDGGAIALGHPLGASGARLLGAAVRQLQCGGGRYALCTMCIGVGQGIATIVERV
>Mature_397_residues
MRQAFLCDAVRTPFGRYGGALAGVRTDDLAAIPLRALLGRNPQLDPGALDDVVYGCVNQAGEDNRNVARMALLLAGIPES
VPGTTVNRLCGSSLEAAAVAARAIRCGEIALAIAGGVESMSRSPLVLSKPDAAWTRSQKIEDSTLGWRFVNPLMKERYGV
DSMAETAENVATECGILRADQDAFAFRSQQRAGAAMTASRLAEEIVAVEISKKPAVSTDEHPRPDTTLEALTALRPIVRP
GGTVTAGNASGINDGAGALLLAGESAAAQYGLTPKARIVATAVAGVAPRAMGLGPVPAVRKVLALTGLTLGQIDLIELNE
AFAAQALAVLRALGLADDAAHVNPDGGAIALGHPLGASGARLLGAAVRQLQCGGGRYALCTMCIGVGQGIATIVERV

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=400, Percent_Identity=42.25, Blast_Score=285, Evalue=5e-77,
Organism=Homo sapiens, GI148539872, Length=396, Percent_Identity=39.3939393939394, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI4501853, Length=395, Percent_Identity=38.9873417721519, Blast_Score=212, Evalue=5e-55,
Organism=Homo sapiens, GI4557237, Length=403, Percent_Identity=34.2431761786601, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI4504327, Length=424, Percent_Identity=33.0188679245283, Blast_Score=184, Evalue=9e-47,
Organism=Homo sapiens, GI194353979, Length=388, Percent_Identity=29.8969072164948, Blast_Score=118, Evalue=8e-27,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=65.3366583541147, Blast_Score=513, Evalue=1e-147,
Organism=Escherichia coli, GI48994986, Length=405, Percent_Identity=44.4444444444444, Blast_Score=290, Evalue=9e-80,
Organism=Escherichia coli, GI1788554, Length=402, Percent_Identity=47.0149253731343, Blast_Score=288, Evalue=6e-79,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=43.3915211970075, Blast_Score=268, Evalue=4e-73,
Organism=Escherichia coli, GI1788683, Length=407, Percent_Identity=34.1523341523341, Blast_Score=171, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI133906874, Length=396, Percent_Identity=40.9090909090909, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI25147385, Length=398, Percent_Identity=32.9145728643216, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17535921, Length=406, Percent_Identity=33.0049261083744, Blast_Score=193, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17551802, Length=428, Percent_Identity=31.0747663551402, Blast_Score=168, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI17535917, Length=403, Percent_Identity=28.7841191066998, Blast_Score=140, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6322031, Length=391, Percent_Identity=37.5959079283887, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6325229, Length=410, Percent_Identity=32.4390243902439, Blast_Score=183, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24655093, Length=400, Percent_Identity=41, Blast_Score=270, Evalue=1e-72,
Organism=Drosophila melanogaster, GI17648125, Length=399, Percent_Identity=40.1002506265664, Blast_Score=248, Evalue=6e-66,
Organism=Drosophila melanogaster, GI17137578, Length=420, Percent_Identity=33.0952380952381, Blast_Score=172, Evalue=5e-43,
Organism=Drosophila melanogaster, GI24640423, Length=403, Percent_Identity=32.258064516129, Blast_Score=168, Evalue=5e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 40711; Mature: 40711

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00737 THIOLASE_2 ; PS00099 THIOLASE_3 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQAFLCDAVRTPFGRYGGALAGVRTDDLAAIPLRALLGRNPQLDPGALDDVVYGCVNQA
CCCHHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHC
GEDNRNVARMALLLAGIPESVPGTTVNRLCGSSLEAAAVAARAIRCGEIALAIAGGVESM
CCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCEEEEEECCHHHH
SRSPLVLSKPDAAWTRSQKIEDSTLGWRFVNPLMKERYGVDSMAETAENVATECGILRAD
CCCCEEEECCCCHHHHCCCCCCHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEECC
QDAFAFRSQQRAGAAMTASRLAEEIVAVEISKKPAVSTDEHPRPDTTLEALTALRPIVRP
CHHHHHHHHHHCCHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
GGTVTAGNASGINDGAGALLLAGESAAAQYGLTPKARIVATAVAGVAPRAMGLGPVPAVR
CCCEECCCCCCCCCCCCEEEEECCHHHHHCCCCCHHHHHHHHHHHHCHHHCCCCCHHHHH
KVLALTGLTLGQIDLIELNEAFAAQALAVLRALGLADDAAHVNPDGGAIALGHPLGASGA
HHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCEECCCCCEEEECCCCCCCHH
RLLGAAVRQLQCGGGRYALCTMCIGVGQGIATIVERV
HHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MRQAFLCDAVRTPFGRYGGALAGVRTDDLAAIPLRALLGRNPQLDPGALDDVVYGCVNQA
CCCHHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHC
GEDNRNVARMALLLAGIPESVPGTTVNRLCGSSLEAAAVAARAIRCGEIALAIAGGVESM
CCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCEEEEEECCHHHH
SRSPLVLSKPDAAWTRSQKIEDSTLGWRFVNPLMKERYGVDSMAETAENVATECGILRAD
CCCCEEEECCCCHHHHCCCCCCHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEECC
QDAFAFRSQQRAGAAMTASRLAEEIVAVEISKKPAVSTDEHPRPDTTLEALTALRPIVRP
CHHHHHHHHHHCCHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
GGTVTAGNASGINDGAGALLLAGESAAAQYGLTPKARIVATAVAGVAPRAMGLGPVPAVR
CCCEECCCCCCCCCCCCEEEEECCHHHHHCCCCCHHHHHHHHHHHHCHHHCCCCCHHHHH
KVLALTGLTLGQIDLIELNEAFAAQALAVLRALGLADDAAHVNPDGGAIALGHPLGASGA
HHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCEECCCCCEEEECCCCCCCHH
RLLGAAVRQLQCGGGRYALCTMCIGVGQGIATIVERV
HHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]