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The map label for this gene is pcaR [H]

Identifier: 116619391

GI number: 116619391

Start: 333705

End: 334481

Strand: Direct

Name: pcaR [H]

Synonym: Acid_0250

Alternate gene names: 116619391

Gene position: 333705-334481 (Clockwise)

Preceding gene: 116619384

Following gene: 116619392

Centisome position: 3.35

GC content: 64.48

Gene sequence:

>777_bases
GTGCTCCAGCTAGAAGCATTCGAGGGCGATCCCGACTTCGTATTGTCGCTGGCGCGCGGGTTGGCGGTGATAGAGGGATT
TCAGGACCGGCCGGATGGCGTTACGGTGGGCGGCGTCGCCGCGCGGACTGGCCTGTCGCGGGCATCCGTGCGGCGGTTGC
TGATTACGCTGGAGCGGTTGGGCTACGCCAGCCACAACGGACCCGTCTACCGGTTGACCAGTCGCGCGCTGCGTCTCGGA
TTCTCCTTCTTATCGTCCAATTCGCTGGCCGCACTGGCGTTGCCGGTGTTGCAGGAAATCAGCGCCGTGATCCACGAGTC
GAGTTCGCTCAGCATGCTGGAAGGCGACGAGATCGTATACCTGGCGCGGTCGGCCACGCAACGTGTGATGTCGGTCGGAC
TCTCGGTGGGCAGCCGGCTACCCGCCTACTGCACATCGATGGGGCGGGTCATGCTGGCCAACCTGACGAAGCCCGAGATC
GCCGCCTATTTGGGACGGGTGGAACTCCAGGCCCGCACGCCCAAGTCGATCGTGAAGAAGGCGGCGTTCGCCGCGGAGTT
GGACCGCGTCCGGCAACAGGGCTACGCGATGGTGGACGAAGAACTCGAACTTGGGCTGCGCTCGTTAGCGGTGCCGGTGC
GGTCGAGATCGGGCAAAGTGGTCGCCGCTATCAACACCGGTGTGCAGGCGGCGCGGAGTACGGCGAAAGAGATGGTCAAA
CGATTTTTGCCCGTACTCCGCGAAGGCGCGGACCGGCTCTCGATCGCGCTTCCATGA

Upstream 100 bases:

>100_bases
CAGGTCTAACAGCTCCATGCGCGAATATCGCCCTATAGCGCGATATTCGCATACTCCCGTCCTCCCGTCAATATTCGCCA
AATTGTGCGATAATCGCCCC

Downstream 100 bases:

>100_bases
GTCGTCGCAACTTGACAAGGCTCGCGCGTTTATCGTACTAATAGCCGATAAGCGCACATCATGCGGCAAGCCTTCCTCTG
CGACGCGGTTCGCACGCCCT

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLQLEAFEGDPDFVLSLARGLAVIEGFQDRPDGVTVGGVAARTGLSRASVRRLLITLERLGYASHNGPVYRLTSRALRLG
FSFLSSNSLAALALPVLQEISAVIHESSSLSMLEGDEIVYLARSATQRVMSVGLSVGSRLPAYCTSMGRVMLANLTKPEI
AAYLGRVELQARTPKSIVKKAAFAAELDRVRQQGYAMVDEELELGLRSLAVPVRSRSGKVVAAINTGVQAARSTAKEMVK
RFLPVLREGADRLSIALP

Sequences:

>Translated_258_residues
MLQLEAFEGDPDFVLSLARGLAVIEGFQDRPDGVTVGGVAARTGLSRASVRRLLITLERLGYASHNGPVYRLTSRALRLG
FSFLSSNSLAALALPVLQEISAVIHESSSLSMLEGDEIVYLARSATQRVMSVGLSVGSRLPAYCTSMGRVMLANLTKPEI
AAYLGRVELQARTPKSIVKKAAFAAELDRVRQQGYAMVDEELELGLRSLAVPVRSRSGKVVAAINTGVQAARSTAKEMVK
RFLPVLREGADRLSIALP
>Mature_258_residues
MLQLEAFEGDPDFVLSLARGLAVIEGFQDRPDGVTVGGVAARTGLSRASVRRLLITLERLGYASHNGPVYRLTSRALRLG
FSFLSSNSLAALALPVLQEISAVIHESSSLSMLEGDEIVYLARSATQRVMSVGLSVGSRLPAYCTSMGRVMLANLTKPEI
AAYLGRVELQARTPKSIVKKAAFAAELDRVRQQGYAMVDEELELGLRSLAVPVRSRSGKVVAAINTGVQAARSTAKEMVK
RFLPVLREGADRLSIALP

Specific function: Positive regulator of all genes within the pca regulon, pcaBDC, pcaIJ and pcaF. Also required for the chemotactic response to aromatic compounds [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1788131, Length=251, Percent_Identity=30.2788844621514, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1789999, Length=249, Percent_Identity=30.5220883534137, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI1786468, Length=247, Percent_Identity=28.3400809716599, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87082359, Length=247, Percent_Identity=26.7206477732794, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1786715, Length=244, Percent_Identity=22.1311475409836, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI145693096, Length=254, Percent_Identity=29.1338582677165, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012794
- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 27739; Mature: 27739

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQLEAFEGDPDFVLSLARGLAVIEGFQDRPDGVTVGGVAARTGLSRASVRRLLITLERL
CCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHCCCHHHHHHHHHHHHHH
GYASHNGPVYRLTSRALRLGFSFLSSNSLAALALPVLQEISAVIHESSSLSMLEGDEIVY
CCCCCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCEEE
LARSATQRVMSVGLSVGSRLPAYCTSMGRVMLANLTKPEIAAYLGRVELQARTPKSIVKK
EHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHEECCCHHHHHHH
AAFAAELDRVRQQGYAMVDEELELGLRSLAVPVRSRSGKVVAAINTGVQAARSTAKEMVK
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHH
RFLPVLREGADRLSIALP
HHHHHHHCCCCCEEEECC
>Mature Secondary Structure
MLQLEAFEGDPDFVLSLARGLAVIEGFQDRPDGVTVGGVAARTGLSRASVRRLLITLERL
CCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHCCCHHHHHHHHHHHHHH
GYASHNGPVYRLTSRALRLGFSFLSSNSLAALALPVLQEISAVIHESSSLSMLEGDEIVY
CCCCCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCEEE
LARSATQRVMSVGLSVGSRLPAYCTSMGRVMLANLTKPEIAAYLGRVELQARTPKSIVKK
EHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHEECCCHHHHHHH
AAFAAELDRVRQQGYAMVDEELELGLRSLAVPVRSRSGKVVAAINTGVQAARSTAKEMVK
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHH
RFLPVLREGADRLSIALP
HHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8083169 [H]