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The map label for this gene is thrA [C]

Identifier: 116619197

GI number: 116619197

Start: 78374

End: 79846

Strand: Direct

Name: thrA [C]

Synonym: Acid_0052

Alternate gene names: 116619197

Gene position: 78374-79846 (Clockwise)

Preceding gene: 116619196

Following gene: 116619198

Centisome position: 0.79

GC content: 65.92

Gene sequence:

>1473_bases
TTGAAAGATCTCCTGGTAATGAAGTTCGGCGGCACCAGCGTGGGCAGCGCCGAGAGAATGAAAGTGGCGGCGCAACTTGC
CGCCCAGGAACAGAAAAAGCGGCCGGTGGCGATCGTGGTGTCGGCGATGAGCAAGGTCACCGACCTGCTGCTGGATACCA
TGCGGCACGCCGAAGCCGGCGATCGCGCGGGAATGGAGCGCAATCTCAACCTGTTGCGCGAGCGCCACGAGCAGGCCTGC
CGGGAGTTGCTGCCGGCCTCCGAACAGGGGGCGGTGCTGGCCAAACTTCACGACGTGATCGACGAGTTCGAGCGCATCGT
CAACGGCATGGCGATGCTCGGCGAGCGTCCGCCGCGGTCGGTAGACGAGGCCGTCGCGGTGGGAGAGCGGCTCACGGTGG
TCCTGGTGAGCGAGCACTTGCGCGTCAGCGGGACGCCCTCGGTGGCGGTCAACGCGCGCGACGTGGTGGTGACGGACGCG
GTCTTCGGTAATGCTTCGCCGTTGATGGAGCCCACGCGTGTGAAGGCTCGCGCCACACTCCGGCCGCAATTGGAGCAGGG
ACTGCTGCCGGTGATCACGGGATTCAACGGCGCCACGGCGGACGGGCGTCCGACCACGCTGGGCCGCGGCGGCAGCGACT
TTTCGGCGTCGATTGTGGCGGCGGCGCTGGACGCGAGCGAATTGTGGATCTGGACCGACGTGGATGGCATCATGAGCGCG
GATCCGCGCCTGGTGCCGGATGCGCAGGTGCTCGAAGAGATCACCTACGCGGAGGCCGCGGAACTGGCCTACAACGGCGC
CAAGGTGCTGCATCCGCGAACGTTGGCGCCGCTGGTGGAGAAGGCGATCCCGGTCTGGAGCAAGAACAGCTTCGCGCCGG
AGAAGCCGGGTACCAAGATCGTGCCCTCCATGGCGGTGGAGCATGGGGCGCGCGCGGTGGCCAGTATGCGCAATGTGGCG
CTGGTCTCGCTGACCCCGGCAAGTCCCGAAGTGAACGGCGTGCAGGTACTGGCGCGGGCGCTCGAAGCCATCGCTCGGGC
GGACGTGGAAGTGCTCGTATTGACCAGTTCCAGCTACCGGCAGAATTTCTGCGTGCTGGTGCGGGAAGACGAGTTGGACC
GCTCGGTGCAGGCGCTGGAATCCGCCCTGGCGCTGGAACTTGCGCACCACTATGTACATCCAATAGAAGTAGATCGTAAC
GTGGGACTGCTGGCCGCCGTGGGCGAGGGCATGCAGGGGAAGCCCGGCCTGGCGGGTCGCATTTTCACTGCCATCTCACG
GGTGCAGGTGAACATCATCGCCATCGCGCAGGGTTCCAGTGAGTTGACGATCGCGGTTGTGGTTCGCCGCGAAGGCCTGG
AGAAGGCCGTCAAAGCGGTGCACGCGGAGTGCGGATTAGGGATGCGTCCACCGGTGCCGGTGTTTCCCTTTGGGGCCTCC
GAAGGACGGGCGCAGCGTCCGACAAAGCGGTAG

Upstream 100 bases:

>100_bases
ACGCGGTGTATCTTGATTCCACCGCGCTGAGCATCGCGGAGGTGGAGGAAGCGATTCTCAAAATCGTGCGTTCGCGCGTG
ACAAACGGAAGGGACTTCAG

Downstream 100 bases:

>100_bases
GGCCCGGTTTCCGATATACTGGAGTTTCCCCTTTAGATCACAGGAGTTCTGTCTAACATGTTTGACCTATTTCGTAGCCG
CGACAAAATGGTGCGCATCC

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MKDLLVMKFGGTSVGSAERMKVAAQLAAQEQKKRPVAIVVSAMSKVTDLLLDTMRHAEAGDRAGMERNLNLLRERHEQAC
RELLPASEQGAVLAKLHDVIDEFERIVNGMAMLGERPPRSVDEAVAVGERLTVVLVSEHLRVSGTPSVAVNARDVVVTDA
VFGNASPLMEPTRVKARATLRPQLEQGLLPVITGFNGATADGRPTTLGRGGSDFSASIVAAALDASELWIWTDVDGIMSA
DPRLVPDAQVLEEITYAEAAELAYNGAKVLHPRTLAPLVEKAIPVWSKNSFAPEKPGTKIVPSMAVEHGARAVASMRNVA
LVSLTPASPEVNGVQVLARALEAIARADVEVLVLTSSSYRQNFCVLVREDELDRSVQALESALALELAHHYVHPIEVDRN
VGLLAAVGEGMQGKPGLAGRIFTAISRVQVNIIAIAQGSSELTIAVVVRREGLEKAVKAVHAECGLGMRPPVPVFPFGAS
EGRAQRPTKR

Sequences:

>Translated_490_residues
MKDLLVMKFGGTSVGSAERMKVAAQLAAQEQKKRPVAIVVSAMSKVTDLLLDTMRHAEAGDRAGMERNLNLLRERHEQAC
RELLPASEQGAVLAKLHDVIDEFERIVNGMAMLGERPPRSVDEAVAVGERLTVVLVSEHLRVSGTPSVAVNARDVVVTDA
VFGNASPLMEPTRVKARATLRPQLEQGLLPVITGFNGATADGRPTTLGRGGSDFSASIVAAALDASELWIWTDVDGIMSA
DPRLVPDAQVLEEITYAEAAELAYNGAKVLHPRTLAPLVEKAIPVWSKNSFAPEKPGTKIVPSMAVEHGARAVASMRNVA
LVSLTPASPEVNGVQVLARALEAIARADVEVLVLTSSSYRQNFCVLVREDELDRSVQALESALALELAHHYVHPIEVDRN
VGLLAAVGEGMQGKPGLAGRIFTAISRVQVNIIAIAQGSSELTIAVVVRREGLEKAVKAVHAECGLGMRPPVPVFPFGAS
EGRAQRPTKR
>Mature_490_residues
MKDLLVMKFGGTSVGSAERMKVAAQLAAQEQKKRPVAIVVSAMSKVTDLLLDTMRHAEAGDRAGMERNLNLLRERHEQAC
RELLPASEQGAVLAKLHDVIDEFERIVNGMAMLGERPPRSVDEAVAVGERLTVVLVSEHLRVSGTPSVAVNARDVVVTDA
VFGNASPLMEPTRVKARATLRPQLEQGLLPVITGFNGATADGRPTTLGRGGSDFSASIVAAALDASELWIWTDVDGIMSA
DPRLVPDAQVLEEITYAEAAELAYNGAKVLHPRTLAPLVEKAIPVWSKNSFAPEKPGTKIVPSMAVEHGARAVASMRNVA
LVSLTPASPEVNGVQVLARALEAIARADVEVLVLTSSSYRQNFCVLVREDELDRSVQALESALALELAHHYVHPIEVDRN
VGLLAAVGEGMQGKPGLAGRIFTAISRVQVNIIAIAQGSSELTIAVVVRREGLEKAVKAVHAECGLGMRPPVPVFPFGAS
EGRAQRPTKR

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=472, Percent_Identity=33.8983050847458, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI1790455, Length=470, Percent_Identity=32.3404255319149, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1790376, Length=465, Percent_Identity=27.741935483871, Blast_Score=119, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6320893, Length=498, Percent_Identity=25.5020080321285, Blast_Score=170, Evalue=5e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 52338; Mature: 52338

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDLLVMKFGGTSVGSAERMKVAAQLAAQEQKKRPVAIVVSAMSKVTDLLLDTMRHAEAG
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
DRAGMERNLNLLRERHEQACRELLPASEQGAVLAKLHDVIDEFERIVNGMAMLGERPPRS
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VDEAVAVGERLTVVLVSEHLRVSGTPSVAVNARDVVVTDAVFGNASPLMEPTRVKARATL
HHHHHHHCCCEEEEEEECCEEECCCCCEEECCCEEEEEEEECCCCCCCCCCHHHHHHHHC
RPQLEQGLLPVITGFNGATADGRPTTLGRGGSDFSASIVAAALDASELWIWTDVDGIMSA
CHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCHHHCC
DPRLVPDAQVLEEITYAEAAELAYNGAKVLHPRTLAPLVEKAIPVWSKNSFAPEKPGTKI
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCC
VPSMAVEHGARAVASMRNVALVSLTPASPEVNGVQVLARALEAIARADVEVLVLTSSSYR
CHHHHHHHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCC
QNFCVLVREDELDRSVQALESALALELAHHYVHPIEVDRNVGLLAAVGEGMQGKPGLAGR
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEECCCCCCCCCCHHH
IFTAISRVQVNIIAIAQGSSELTIAVVVRREGLEKAVKAVHAECGLGMRPPVPVFPFGAS
HHHHHHHEEEEEEEEECCCCCEEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCEECCCCC
EGRAQRPTKR
CCCCCCCCCC
>Mature Secondary Structure
MKDLLVMKFGGTSVGSAERMKVAAQLAAQEQKKRPVAIVVSAMSKVTDLLLDTMRHAEAG
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
DRAGMERNLNLLRERHEQACRELLPASEQGAVLAKLHDVIDEFERIVNGMAMLGERPPRS
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VDEAVAVGERLTVVLVSEHLRVSGTPSVAVNARDVVVTDAVFGNASPLMEPTRVKARATL
HHHHHHHCCCEEEEEEECCEEECCCCCEEECCCEEEEEEEECCCCCCCCCCHHHHHHHHC
RPQLEQGLLPVITGFNGATADGRPTTLGRGGSDFSASIVAAALDASELWIWTDVDGIMSA
CHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCHHHCC
DPRLVPDAQVLEEITYAEAAELAYNGAKVLHPRTLAPLVEKAIPVWSKNSFAPEKPGTKI
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCC
VPSMAVEHGARAVASMRNVALVSLTPASPEVNGVQVLARALEAIARADVEVLVLTSSSYR
CHHHHHHHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCC
QNFCVLVREDELDRSVQALESALALELAHHYVHPIEVDRNVGLLAAVGEGMQGKPGLAGR
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEECCCCCCCCCCHHH
IFTAISRVQVNIIAIAQGSSELTIAVVVRREGLEKAVKAVHAECGLGMRPPVPVFPFGAS
HHHHHHHEEEEEEEEECCCCCEEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCEECCCCC
EGRAQRPTKR
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]