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The map label for this gene is tapB [H]

Identifier: 116619190

GI number: 116619190

Start: 71430

End: 73055

Strand: Direct

Name: tapB [H]

Synonym: Acid_0045

Alternate gene names: 116619190

Gene position: 71430-73055 (Clockwise)

Preceding gene: 116619189

Following gene: 116619191

Centisome position: 0.72

GC content: 59.78

Gene sequence:

>1626_bases
ATGGCGACGATGGACAAGGCGAAATTATCGGAACCCGGGGCGGAGCAGTCCCAGGCGCAGGCCATCGCCGCGCGGTATCG
CTGCGAATATGTGGACCTGCGGGAGGCGGGGATCGATCACGATCTCTTCCGCTCGATTCCCGTGGACCTGATGTTCCGCT
ACAATTTCGTGCCGTTGCACGCGCAGAACGGCACCCTGGACATCGCACTTTCCGACCCGCGGAATCTCAACCTGATCGAC
GAATTGACGCTGCTGCTCAATAAGAAGCTGCGCGTTAAAGTGGCCACGCTTTCGCAGATTTCGGACCTGCTCAAGAAAAC
CGAGCAATCGCAGCGCGTCCTGGAGGAAGTCACCGAGGGCTTCGCGCTGGACGTGGTGGTGGAGGACGGCGATTCCGACG
AGACGCTCTCCATCGACAAACTGACCGCGACCGATTCGGACATCGCGCCGGTCATCAAACTGGTGGACACCACGATCTTC
AACGCCCTGGAGCGGCGGGCCAGCGATATTCATATCGAATCGCGCGACCAGGAAGTGGCCATCAAGTACCGCATCGATGG
CGTGCTGCACTATGCGATGCCGCCGATCGCGAAGGACTGGCAGTCCACCATTATTTCGCGCATCAAGGTGATGAGCGAGT
TGGATATCGCCGAAAAGCGCGTGCCGCAGGACGGCCGTTTCCGGGTGCGCTACAAGAATCGCCTGATCGATTTCCGCGTC
TCCATCATGCCCACGATTCACGGCGAAGACGCCGTGCTCCGCGTCTTGGACAAGGAATCGATGAGCGAGAAGTTCGCCAA
GCTTTCGCTCGACGTGGTGGGCTTCAGCGAGGCCGACCTGGTCAAGTTCCGGCGCTACATTATGGAGCCGTACGGCATGG
TGCTGGTCACGGGTCCTACGGGATCCGGCAAGACGACCACGTTGTACGCCGCGCTGAGCGAGATCAAGAACGACGAAGAC
AAGATCATCACCATCGAAGACCCGGTGGAATATCAGTTGAAGGGGATCACGCAGATTCCGGTGAATGACAAGAAGGGGCT
GACCTTCGCCCGTGGACTGCGCTCGATTCTGCGCCACGATCCGGACAAGATCATGGTGGGCGAAATCCGCGACCAGGAGA
CGGCGCAGATCGCCATCAACTCCGCGCTCACCGGCCACCTGGTGTTCACCACGGTGCACGCGAACAACGTGCTCGACGTG
CTGGGACGCTTCCTGAACATGGGCGTGGAGGCGTACAACTTCGTTTCCGCGCTGAATTGCATTCTGGCGCAGCGCCTGGT
GCGGCAGATCTGCGAGCACTGTAAGCGCGAGGTCAAATACCCGGATTCGCTGATGGTGGAGAGCGCGCTGAATCCCGACG
AGTGGCGGGACGTCCCGTTATACGAAGGCGCAGGGTGCTTCGAATGCGGGGGAACCGGTTTCCGGGGACGCTCGGCGATC
CACGAACTGCTGGATCTGTCTGACCGGATTCGCGAAATGATTCTGGATAAGCGGCCGACGTCCGAGATCAAGCGGGCGGC
GCGCGAAGAAGGCATGACCTTCCTTCGGGAATCGGCGGTAGACAAAATGCGCCTGGGGATTACGACCCTGCGCGAGATCA
ATAAGGTCACATTCATTGAAGGGTAA

Upstream 100 bases:

>100_bases
GCGATCATGATCGTCATGGGTGGAGTGGTGGCGTTCGTATTGATTGCGCTGTATCTGCCGATCTTCTCACTGGCGGACAC
CATTCGGTAAGGGTTGAATT

Downstream 100 bases:

>100_bases
AATGCAAACACATTTCTCGCAGAGACGCAGAGACGCAGAAAACCGCAAAGAAAACCTGAAGTCTTTCTTCTTTGCGTTAC
TTTGCGGCTTTGCGCCTTTG

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 541; Mature: 540

Protein sequence:

>541_residues
MATMDKAKLSEPGAEQSQAQAIAARYRCEYVDLREAGIDHDLFRSIPVDLMFRYNFVPLHAQNGTLDIALSDPRNLNLID
ELTLLLNKKLRVKVATLSQISDLLKKTEQSQRVLEEVTEGFALDVVVEDGDSDETLSIDKLTATDSDIAPVIKLVDTTIF
NALERRASDIHIESRDQEVAIKYRIDGVLHYAMPPIAKDWQSTIISRIKVMSELDIAEKRVPQDGRFRVRYKNRLIDFRV
SIMPTIHGEDAVLRVLDKESMSEKFAKLSLDVVGFSEADLVKFRRYIMEPYGMVLVTGPTGSGKTTTLYAALSEIKNDED
KIITIEDPVEYQLKGITQIPVNDKKGLTFARGLRSILRHDPDKIMVGEIRDQETAQIAINSALTGHLVFTTVHANNVLDV
LGRFLNMGVEAYNFVSALNCILAQRLVRQICEHCKREVKYPDSLMVESALNPDEWRDVPLYEGAGCFECGGTGFRGRSAI
HELLDLSDRIREMILDKRPTSEIKRAAREEGMTFLRESAVDKMRLGITTLREINKVTFIEG

Sequences:

>Translated_541_residues
MATMDKAKLSEPGAEQSQAQAIAARYRCEYVDLREAGIDHDLFRSIPVDLMFRYNFVPLHAQNGTLDIALSDPRNLNLID
ELTLLLNKKLRVKVATLSQISDLLKKTEQSQRVLEEVTEGFALDVVVEDGDSDETLSIDKLTATDSDIAPVIKLVDTTIF
NALERRASDIHIESRDQEVAIKYRIDGVLHYAMPPIAKDWQSTIISRIKVMSELDIAEKRVPQDGRFRVRYKNRLIDFRV
SIMPTIHGEDAVLRVLDKESMSEKFAKLSLDVVGFSEADLVKFRRYIMEPYGMVLVTGPTGSGKTTTLYAALSEIKNDED
KIITIEDPVEYQLKGITQIPVNDKKGLTFARGLRSILRHDPDKIMVGEIRDQETAQIAINSALTGHLVFTTVHANNVLDV
LGRFLNMGVEAYNFVSALNCILAQRLVRQICEHCKREVKYPDSLMVESALNPDEWRDVPLYEGAGCFECGGTGFRGRSAI
HELLDLSDRIREMILDKRPTSEIKRAAREEGMTFLRESAVDKMRLGITTLREINKVTFIEG
>Mature_540_residues
ATMDKAKLSEPGAEQSQAQAIAARYRCEYVDLREAGIDHDLFRSIPVDLMFRYNFVPLHAQNGTLDIALSDPRNLNLIDE
LTLLLNKKLRVKVATLSQISDLLKKTEQSQRVLEEVTEGFALDVVVEDGDSDETLSIDKLTATDSDIAPVIKLVDTTIFN
ALERRASDIHIESRDQEVAIKYRIDGVLHYAMPPIAKDWQSTIISRIKVMSELDIAEKRVPQDGRFRVRYKNRLIDFRVS
IMPTIHGEDAVLRVLDKESMSEKFAKLSLDVVGFSEADLVKFRRYIMEPYGMVLVTGPTGSGKTTTLYAALSEIKNDEDK
IITIEDPVEYQLKGITQIPVNDKKGLTFARGLRSILRHDPDKIMVGEIRDQETAQIAINSALTGHLVFTTVHANNVLDVL
GRFLNMGVEAYNFVSALNCILAQRLVRQICEHCKREVKYPDSLMVESALNPDEWRDVPLYEGAGCFECGGTGFRGRSAIH
ELLDLSDRIREMILDKRPTSEIKRAAREEGMTFLRESAVDKMRLGITTLREINKVTFIEG

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=432, Percent_Identity=41.2037037037037, Blast_Score=330, Evalue=1e-91,
Organism=Escherichia coli, GI1786296, Length=489, Percent_Identity=36.6053169734151, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI87082188, Length=119, Percent_Identity=44.5378151260504, Blast_Score=99, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 61035; Mature: 60904

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATMDKAKLSEPGAEQSQAQAIAARYRCEYVDLREAGIDHDLFRSIPVDLMFRYNFVPLH
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEHHHCCCCHHHHHHCCCEEEEEEEEEEEE
AQNGTLDIALSDPRNLNLIDELTLLLNKKLRVKVATLSQISDLLKKTEQSQRVLEEVTEG
ECCCEEEEEECCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCC
FALDVVVEDGDSDETLSIDKLTATDSDIAPVIKLVDTTIFNALERRASDIHIESRDQEVA
EEEEEEEECCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCEEE
IKYRIDGVLHYAMPPIAKDWQSTIISRIKVMSELDIAEKRVPQDGRFRVRYKNRLIDFRV
EEEEECEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEE
SIMPTIHGEDAVLRVLDKESMSEKFAKLSLDVVGFSEADLVKFRRYIMEPYGMVLVTGPT
EEEEEECCCHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHCCCCEEEEECCC
GSGKTTTLYAALSEIKNDEDKIITIEDPVEYQLKGITQIPVNDKKGLTFARGLRSILRHD
CCCCHHHHHHHHHHHCCCCCCEEEEECCCCHHCCCCEECCCCCCCCCHHHHHHHHHHHCC
PDKIMVGEIRDQETAQIAINSALTGHLVFTTVHANNVLDVLGRFLNMGVEAYNFVSALNC
CCCEEEECCCCCCHHHEEECCCCCCEEEEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHH
ILAQRLVRQICEHCKREVKYPDSLMVESALNPDEWRDVPLYEGAGCFECGGTGFRGRSAI
HHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCCCHHHHH
HELLDLSDRIREMILDKRPTSEIKRAAREEGMTFLRESAVDKMRLGITTLREINKVTFIE
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEC
G
C
>Mature Secondary Structure 
ATMDKAKLSEPGAEQSQAQAIAARYRCEYVDLREAGIDHDLFRSIPVDLMFRYNFVPLH
CCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEHHHCCCCHHHHHHCCCEEEEEEEEEEEE
AQNGTLDIALSDPRNLNLIDELTLLLNKKLRVKVATLSQISDLLKKTEQSQRVLEEVTEG
ECCCEEEEEECCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCC
FALDVVVEDGDSDETLSIDKLTATDSDIAPVIKLVDTTIFNALERRASDIHIESRDQEVA
EEEEEEEECCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCEEE
IKYRIDGVLHYAMPPIAKDWQSTIISRIKVMSELDIAEKRVPQDGRFRVRYKNRLIDFRV
EEEEECEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEE
SIMPTIHGEDAVLRVLDKESMSEKFAKLSLDVVGFSEADLVKFRRYIMEPYGMVLVTGPT
EEEEEECCCHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHCCCCEEEEECCC
GSGKTTTLYAALSEIKNDEDKIITIEDPVEYQLKGITQIPVNDKKGLTFARGLRSILRHD
CCCCHHHHHHHHHHHCCCCCCEEEEECCCCHHCCCCEECCCCCCCCCHHHHHHHHHHHCC
PDKIMVGEIRDQETAQIAINSALTGHLVFTTVHANNVLDVLGRFLNMGVEAYNFVSALNC
CCCEEEECCCCCCHHHEEECCCCCCEEEEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHH
ILAQRLVRQICEHCKREVKYPDSLMVESALNPDEWRDVPLYEGAGCFECGGTGFRGRSAI
HHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCCCHHHHH
HELLDLSDRIREMILDKRPTSEIKRAAREEGMTFLRESAVDKMRLGITTLREINKVTFIE
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8820654 [H]