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The map label for this gene is supH [H]

Identifier: 116516695

GI number: 116516695

Start: 1305868

End: 1306695

Strand: Reverse

Name: supH [H]

Synonym: SPD_1280

Alternate gene names: 116516695

Gene position: 1306695-1305868 (Counterclockwise)

Preceding gene: 116515589

Following gene: 116515324

Centisome position: 63.86

GC content: 43.96

Gene sequence:

>828_bases
ATGATGATTAAGGTAATTGCGACAGATATGGATGGGACCTTGCTGGATGCTAGAGGTCAGCTTGATCTCCCACGATTGGA
AAAGATTTTAGATCAGTTGGATCAAAGGGGCATTCGTTTTGTCATTGCGACGGGCAATGAAATTCACCGCATGAGACAAC
TACTGAGTCCCTTGGTGGATCGAGTGGTTCTGGTTGTTGCTAATGGCGCTCGTATTTTTGAAAACAATGAATTGATTCAG
GCTCAGACATGGGATGACGCCATTGTCAACAAGGCTTTGACTCATTTCAAGGGTCGAGCGTGTCAGGACCAGTTTGTTGT
AACGGGGATGAAGGGTGATTTTGTCAAGGAAGGTACGATTTTTACAGATCTTGAAAGTTTTATGACTCCAGAAATGATTG
AAAAATTCTACCAACGGATGCAATTTGTGGATGAATTAACATCTGACCTCTTTGGTGGTGTGCTCAAGATGAGCATGGTT
GTTGGTGAGGAACGTTTGAGTTCGGTTTTGGAAGAAATCAATGCTCTCTTTGATGGCCGTGTCCGAGCTGTATCCAGTGG
CTATGGTTGCATTGATATCCTCCAAGCTGGGATTCATAAAGCATGGGGCTTGGAGGAATTACTCAAGCGCTGGGACTTGA
AATCCCAAGAAATCATGGCTTTTGGTGATAGTGAAAATGATGTTGAAATGCTTGAAATGGCTGGAATTGCCTATGCGATG
GAAAATGCTGATGAGAAAGCCAAAGCTGTGGCGACTGCTCTAGCACCAGCCAACAGCCAAGGAGGAGTTTATCAAGTCTT
GGAAAACTGGTTAGAAAAAGGAGAATGA

Upstream 100 bases:

>100_bases
GTTAGATGAAGCGACTAAGGAACGAATTGATACAGCCTATAAAGAAATGAATGAACATGTGGGATTGAAGTGGTATTAGC
TTGAATCCAGAGGAAAGTAA

Downstream 100 bases:

>100_bases
AGTGGCAGTACAGTTATTAGAAAATTGGCTCCTAAAGGAACAAGAAAAAATTCAAACTAAGTATCGTCACCTAAATCACA
TTTCTGTTGTAGAACCAAAC

Product: Cof family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIATGNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQ
AQTWDDAIVNKALTHFKGRACQDQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEIMAFGDSENDVEMLEMAGIAYAM
ENADEKAKAVATALAPANSQGGVYQVLENWLEKGE

Sequences:

>Translated_275_residues
MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIATGNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQ
AQTWDDAIVNKALTHFKGRACQDQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEIMAFGDSENDVEMLEMAGIAYAM
ENADEKAKAVATALAPANSQGGVYQVLENWLEKGE
>Mature_275_residues
MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIATGNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQ
AQTWDDAIVNKALTHFKGRACQDQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEIMAFGDSENDVEMLEMAGIAYAM
ENADEKAKAVATALAPANSQGGVYQVLENWLEKGE

Specific function: Catalyzes the hydrolysis of sugar phosphate to sugar and inorganic phosphate. Has a wide substrate specificity catalyzing the hydrolysis of fructose-1-P most efficiently, but it remains uncertain if this is the real substrate in vivo [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family. SupH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787043, Length=275, Percent_Identity=32.7272727272727, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI87081790, Length=281, Percent_Identity=29.5373665480427, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI48994981, Length=260, Percent_Identity=29.6153846153846, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI2367265, Length=273, Percent_Identity=25.6410256410256, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI87081741, Length=290, Percent_Identity=26.2068965517241, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR013200
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF08282 Hydrolase_3 [H]

EC number: =3.1.3.23 [H]

Molecular weight: Translated: 30759; Mature: 30759

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIATGNEIHRMRQLLSPLVD
CEEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHH
RVVLVVANGARIFENNELIQAQTWDDAIVNKALTHFKGRACQDQFVVTGMKGDFVKEGTI
HHHHHHCCCCEEECCCCEEEECCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEECCCH
FTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMVVGEERLSSVLEEINALFDGR
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
VRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEIMAFGDSENDVEMLEMAGIAYAM
HHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHH
ENADEKAKAVATALAPANSQGGVYQVLENWLEKGE
HCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIATGNEIHRMRQLLSPLVD
CEEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHH
RVVLVVANGARIFENNELIQAQTWDDAIVNKALTHFKGRACQDQFVVTGMKGDFVKEGTI
HHHHHHCCCCEEECCCCEEEECCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEECCCH
FTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMVVGEERLSSVLEEINALFDGR
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
VRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEIMAFGDSENDVEMLEMAGIAYAM
HHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHH
ENADEKAKAVATALAPANSQGGVYQVLENWLEKGE
HCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]