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The map label for this gene is rrmA [H]

Identifier: 116516682

GI number: 116516682

Start: 1900004

End: 1900852

Strand: Direct

Name: rrmA [H]

Synonym: SPD_1929

Alternate gene names: 116516682

Gene position: 1900004-1900852 (Clockwise)

Preceding gene: 116516055

Following gene: 116515564

Centisome position: 92.86

GC content: 42.05

Gene sequence:

>849_bases
ATGAATACAAATCTCAAGCCCAAACTTCAGCGTTTTGCTTCTGCGACTGCCTTTGCCTGTCCTATCTGTCAAGAAAATCT
GACTCTGTTAGAGACTAATTTCAAGTGCTGCAACCGTCATTCTTTTGACTTGGCGAAATTTGGCTATGTCAATCTAGCAC
CTCAAATCAAGCAATCTGCTAACTATGACAAGGAAAATTTTCAAAACCGTCAACAAATCCTAGAAGCCGGCTTTTACCAA
GCTATCTTAGATGCTGTATCTGACTTGCTTGCAAGCTCAAAAACTACCACAACAATTTTGGATATCGGTTGTGGTGAAGG
ATTCTATTCTCGCAAACTACAAGAAAGTCACTCTGAAAAAACTTTCTATGCCTTTGACATCTCCAAAGATTCAGTCCAAA
TCGCTGCTAAAAGTGAACCCAACTGGGCAGTCAATTGGTTCGTTGGCGACTTGGCACGCCTTCCTATAAAAGACGCTAGC
ATGGATATCCTGCTTGATATCTTCTCACCTGCCAACTATGGAGAATTTCGTCGCGTTTTATCCAAAGACGGTATCTTGAT
AAAGGTTATCCCAACTGAAAATCACCTCAAAGAAATCCGTCAAAGAGTACAGGACCAGCTGACAAACAAGGAGTATTCTA
ACCAAGATATCAAGGAGCATTTCCAGGAACACTTTACCATCCTATCTAGTCAAACTGCCTCTCTGACTAAGACTATCACA
GCAGAACAACTCCAAGCCCTACTCAGTATGACTCCTCTCCTCTTTCACGTTGACCAGAGCAAGATTGACTGGAGCCAACT
GACAGAGATTACCATCGAAGCAGAGATTTTGGTTGGGAAAGCATTCTAA

Upstream 100 bases:

>100_bases
TTCATTCTATAAATTGGAGAATTAGTTTTATTTCTACTGAATCTCCCATCTACTTATTCCTAAAGTTGCTTTCATTTGCC
TCTTTTGATACACTTAAACT

Downstream 100 bases:

>100_bases
TACTCTTCAAACCACGTCAACGTCGCCTTGCCGTACGTATGGTTACTGACTTCGTCAGTTTCATCTACAACCTCAAAACC
ATGTTTTGAGCTGACTTCGT

Product: rRNA (guanine-N1-)-methyltransferase

Products: S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDLAKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQ
AILDAVSDLLASSKTTTTILDIGCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAS
MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIKEHFQEHFTILSSQTASLTKTIT
AEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEILVGKAF

Sequences:

>Translated_282_residues
MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDLAKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQ
AILDAVSDLLASSKTTTTILDIGCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAS
MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIKEHFQEHFTILSSQTASLTKTIT
AEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEILVGKAF
>Mature_282_residues
MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDLAKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQ
AILDAVSDLLASSKTTTTILDIGCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAS
MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIKEHFQEHFTILSSQTASLTKTIT
AEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEILVGKAF

Specific function: Specifically Methylates The Guanosine In Position 745 Of 23s rRNA. [C]

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmA family [H]

Homologues:

Organism=Escherichia coli, GI1788125, Length=244, Percent_Identity=33.1967213114754, Blast_Score=140, Evalue=9e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR016718 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: 2.1.1.51 [C]

Molecular weight: Translated: 32000; Mature: 32000

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDLAKFGYVNLAPQIKQSA
CCCCCCHHHHHHHHHHHHCCCHHHCCCEEEECCHHHHCCCCCCHHHCCCCCCCHHHHHHC
NYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDIGCGEGFYSRKLQESHSEK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCC
TFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDASMDILLDIFSPANYGEFRRVL
EEEEEEECCCCEEEEECCCCCEEEEEEHHHHHHCCCCCCCHHHEEEECCCCCHHHHHHHH
SKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIKEHFQEHFTILSSQTASLTKTIT
CCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
AEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEILVGKAF
HHHHHHHHHHCHHEEECCCCCCCHHHHHEEEEEEEEEEECCC
>Mature Secondary Structure
MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDLAKFGYVNLAPQIKQSA
CCCCCCHHHHHHHHHHHHCCCHHHCCCEEEECCHHHHCCCCCCHHHCCCCCCCHHHHHHC
NYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDIGCGEGFYSRKLQESHSEK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCC
TFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDASMDILLDIFSPANYGEFRRVL
EEEEEEECCCCEEEEECCCCCEEEEEEHHHHHHCCCCCCCHHHEEEECCCCCHHHHHHHH
SKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIKEHFQEHFTILSSQTASLTKTIT
CCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
AEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEILVGKAF
HHHHHHHHHHCHHEEECCCCCCCHHHHHEEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Unidentified protein factor B [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; rRNA [C]

Specific reaction: S-Adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

General reaction: Methyl group transfer [C]

Inhibitor: Mg2+; Unidentified protein factor A1 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969509; 9384377 [H]