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The map label for this gene is recF

Identifier: 116516540

GI number: 116516540

Start: 2029900

End: 2030997

Strand: Direct

Name: recF

Synonym: SPD_2054

Alternate gene names: 116516540

Gene position: 2029900-2030997 (Clockwise)

Preceding gene: 116515378

Following gene: 116515858

Centisome position: 99.21

GC content: 34.34

Gene sequence:

>1098_bases
ATGTGGCTACAACATCTATCTCTCAAGACTTTTCGTAATTACAAAGAAACGAAAATAGACTTTAATCCTAAATTGAATGT
CTTTTTGGGACGTAATGCACAAGGAAAAACAAACATGTTAGAGGCTATCTATTTTTTAGCCTTAACGCGTAGTCATCGAA
CTCGAACAGATAAAAATCTCATTCATTTTGATGAGGAACAACTTCATCTTTCAGGTCTCGTTCAGAAAAAAACTGGATCC
ATTCCCCTAGAAATCGAACTAACACAAAAAGGCCGTGTAACAAAAGTTAATCACTTAAAACAGGCACGCCTTTCAGATTA
TGTAGGACACATGAATGTTGTCTTATTTGCTCCTGAAGATTTACAACTAATTAAAGGAGCACCTTCGATTCGACGAAAAT
TCATTGATATGGAACTTGGGCAAATTAAGCCAATCTATTTATCTGACTTAACCAATTATAACCACATCCTAAAGCAAAGA
AACACTTACCTAAAATCAGCTCAAAAAATAGATGAAACATTCCTTTCTGTGTTAGATGATCAGCTAGTTGATTATGGATG
TCGTGTAATGAATCACCGCTTAGATTTCATAAAAAAACTAGAATCATTTGGGCGTAAGAAACATTTTGAACTCTCTAATC
AGATCGAAGAGTTGTCAATATCCTATCAATCTTCTGTCAATATAACTGACAAACAAAACTTATCCGAATCTTTCAAAATT
GCTTTAGAAAAAAGTAGATCCAGAGATTTATTTAAAAAGAATACTGGTGTCGGTCCTCATCGAGATGACATTTCTTTTTA
TATAAATGGGATGGATGCTAGTTTCGGAAGCCAAGGTCAACATCGTAGTCTCGTCCTCTCGATAAAATTAGCAGAAATCG
AATTAATGGAAAGCATTACCACAGAATCTCCTATATTACTGCTTGACGATGTGATGAGTGAACTTGACAACACTAGACAG
TTAAAATTATTAGAAACGATTTCTCAATCAATCCAAACCTTTATCACAACAACAAGCTTAGACCATCTTCAAAATCTGCC
AGAAAATCTAAGTATCTTCACTATTCAGGATGGTAAAGCTTCTGTAAATGGAAATTGA

Upstream 100 bases:

>100_bases
AACTTGTCAAAAAGATGAATAAGGGAGTTAAAAAAGACAAATCGAAACCTGCTTCATCACCTAAAAGCCAACAAGCTCCA
CGATTCCCTGGTAGATAATC

Downstream 100 bases:

>100_bases
CAACATTGTAAAAGAACTCCTGTTTTCACTGGAGTTCTTTTCATTGTTTTTTACATAGAATAATTTGGTGCCTCATTAGT
AATTTGCACATCATGAGGAT

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIYFLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGS
IPLEIELTQKGRVTKVNHLKQARLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEELSISYQSSVNITDKQNLSESFKI
ALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFGSQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQ
LKLLETISQSIQTFITTTSLDHLQNLPENLSIFTIQDGKASVNGN

Sequences:

>Translated_365_residues
MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIYFLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGS
IPLEIELTQKGRVTKVNHLKQARLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEELSISYQSSVNITDKQNLSESFKI
ALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFGSQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQ
LKLLETISQSIQTFITTTSLDHLQNLPENLSIFTIQDGKASVNGN
>Mature_365_residues
MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIYFLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGS
IPLEIELTQKGRVTKVNHLKQARLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEELSISYQSSVNITDKQNLSESFKI
ALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFGSQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQ
LKLLETISQSIQTFITTTSLDHLQNLPENLSIFTIQDGKASVNGN

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=374, Percent_Identity=24.5989304812834, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_STRP2 (Q04HV1)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_817437.1
- ProteinModelPortal:   Q04HV1
- STRING:   Q04HV1
- EnsemblBacteria:   EBSTRT00000019498
- GeneID:   4441043
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_2054
- eggNOG:   COG1195
- GeneTree:   EBGT00050000028130
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   PRK00064
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 41917; Mature: 41917

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIYFLALTRSHRTRTDKNL
CCCHHHHHHHHHCCHHCEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
IHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQARLSDYVGHMNVVLFAPED
EECCHHHHHHHHHHHHCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHHCCEEEEEECCCC
LQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQRNTYLKSAQKIDETFLSVLDD
HHHHCCCHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEELSISYQSSVNITDKQNLSESFKI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHEECCCCCCCCCHHCCHHHHHH
ALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFGSQGQHRSLVLSIKLAEIELMESIT
HHHHHCCHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCCEEEEEEEEHHHHHHHHHC
TESPILLLDDVMSELDNTRQLKLLETISQSIQTFITTTSLDHLQNLPENLSIFTIQDGKA
CCCCEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEEEEECCCC
SVNGN
CCCCC
>Mature Secondary Structure
MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIYFLALTRSHRTRTDKNL
CCCHHHHHHHHHCCHHCEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
IHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQARLSDYVGHMNVVLFAPED
EECCHHHHHHHHHHHHCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHHCCEEEEEECCCC
LQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQRNTYLKSAQKIDETFLSVLDD
HHHHCCCHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEELSISYQSSVNITDKQNLSESFKI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHEECCCCCCCCCHHCCHHHHHH
ALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFGSQGQHRSLVLSIKLAEIELMESIT
HHHHHCCHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCCEEEEEEEEHHHHHHHHHC
TESPILLLDDVMSELDNTRQLKLLETISQSIQTFITTTSLDHLQNLPENLSIFTIQDGKA
CCCCEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEEEEECCCC
SVNGN
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA