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The map label for this gene is guaA

Identifier: 116516474

GI number: 116516474

Start: 1300137

End: 1301699

Strand: Reverse

Name: guaA

Synonym: SPD_1274

Alternate gene names: 116516474

Gene position: 1301699-1300137 (Counterclockwise)

Preceding gene: 116516351

Following gene: 116517166

Centisome position: 63.62

GC content: 42.16

Gene sequence:

>1563_bases
ATGAGCAACATTTCAACTGATTTGCAAGATGTAGAAAAAATCATCGTATTGGACTATGGTAGCCAGTACAACCAGCTGAT
TTCACGCCGTATCCGTGAGATTGGTGTTTTTTCAGAACTAAAAAGCCATAAAATTTCAGCTGCTGAAGTTCGTGAAGTCA
ATCCTGTAGGAATTATTCTATCAGGTGGTCCAAATTCTGTATATGAAGATGGTTCATTTGATATTGACCCAGAAATCTTC
GAACTCGGAATTCCAATTTTGGGAATCTGTTATGGTATGCAGTTATTGACCCATAAACTTGGAGGAAAAGTTGTTCCTGC
AGGTGATGCTGGAAATCGTGAATACGGTCAATCAACCCTAACTCACACACCATCAGCGCTTTTTGAATCAACACCTGATG
AACAGACTGTTTTGATGAGCCATGGTGATGCGGTTACTGAGATTCCTGCTGACTTTGTTCGTACAGGTACATCAGCTGAC
TGCCCATACGCAGCCATCGAAAACCCAGATAAACACATTTACGGTATCCAATTCCACCCAGAAGTTCGTCATTCTGTATA
CGGAAATGATATCCTTCGTAACTTTGCCCTTAACATTTGTAAGGCTAAAGGTGACTGGTCAATGGATAATTTCATTGACA
TGCAGATCAAAAAAATTCGTGAAACCGTCGGTGATAAACGTGTCCTTCTTGGTCTATCAGGTGGTGTTGACTCATCTGTC
GTTGGGGTTCTTCTCCAAAAAGCGATTGGCGATCAATTGATCTGTATCTTCGTAGACCACGGTCTTCTTCGTAAAGGCGA
AGCTGATCAAGTTATGGACATGCTCGGTGGTAAGTTTGGTTTGAATATCGTCAAAGCAGACGCTGCTAAACGTTTCCTTG
ACAAACTTGCTGGCGTTTCTGATCCTGAACAAAAACGTAAAATCATTGGTAACGAGTTTGTCTATGTATTCGATGACGAA
GCAAGCAAGCTCAAAGATGTGAAATTCCTTGCTCAAGGTACTTTATATACAGATGTTATCGAGTCTGGTACGGATACAGC
TCAAACTATCAAGTCACACCACAACGTGGGTGGTCTTCCAGAAGATATGCAGTTTGAATTGATTGAACCACTCAATACTC
TTTACAAGGATGAAGTTCGTGCTCTTGGTACAGAGCTTGGTATGCCAGACCATATCGTATGGCGCCAACCATTCCCAGGA
CCAGGACTTGCTATCCGTGTCATGGGTGAAATCACTGAAGAGAAACTTGAAACCGTTCGTGAATCAGACGCTATTCTTCG
TGAAGAAATCGCTAAAGCTGGACTTGACCGCGATATTTGGCAATACTTCACTGTTAACACAGGCGTTCGTTCAGTCGGTG
TTATGGGTGACGGTCGTACGTATGACTACACGATTGCAATCCGTGCTATCACTTCTATCGATGGTATGACTGCTGATTTT
GCCAAAATTCCATGGGAAGTACTTCAAAAAATCTCAGTACGTATCGTAAATGAAGTGGATCATGTTAACCGTATCGTCTA
CGATATTACAAGTAAACCACCTGCAACAGTTGAGTGGGAGTAA

Upstream 100 bases:

>100_bases
TAAACTAGAAAAAGTCAAACTTCGCCTTGTTTAGTTGGTAATTCGCCCTTATTTGTGATAGAATATTGAGAAAAGATATT
TCTTTTGAGAAAGGAAAAAG

Downstream 100 bases:

>100_bases
TCGCAAAAAAATTAAAAGCTTTGTAAAATCAACGGTTACAGAGGATTAAAAACTGTAACTGGGATTAAAACGGGAACATT
TACTAAAAAGAATAAATTGA

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHKISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIF
ELGIPILGICYGMQLLTHKLGGKVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKKIRETVGDKRVLLGLSGGVDSSV
VGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLGGKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDE
ASKLKDVKFLAQGTLYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHIVWRQPFPG
PGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVRSVGVMGDGRTYDYTIAIRAITSIDGMTADF
AKIPWEVLQKISVRIVNEVDHVNRIVYDITSKPPATVEWE

Sequences:

>Translated_520_residues
MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHKISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIF
ELGIPILGICYGMQLLTHKLGGKVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKKIRETVGDKRVLLGLSGGVDSSV
VGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLGGKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDE
ASKLKDVKFLAQGTLYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHIVWRQPFPG
PGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVRSVGVMGDGRTYDYTIAIRAITSIDGMTADF
AKIPWEVLQKISVRIVNEVDHVNRIVYDITSKPPATVEWE
>Mature_519_residues
SNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHKISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFE
LGIPILGICYGMQLLTHKLGGKVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSADC
PYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKKIRETVGDKRVLLGLSGGVDSSVV
GVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLGGKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEA
SKLKDVKFLAQGTLYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHIVWRQPFPGP
GLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVRSVGVMGDGRTYDYTIAIRAITSIDGMTADFA
KIPWEVLQKISVRIVNEVDHVNRIVYDITSKPPATVEWE

Specific function: Catalyzes the synthesis of GMP from XMP

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain

Homologues:

Organism=Homo sapiens, GI4504035, Length=568, Percent_Identity=34.330985915493, Blast_Score=282, Evalue=4e-76,
Organism=Escherichia coli, GI1788854, Length=521, Percent_Identity=52.0153550863724, Blast_Score=550, Evalue=1e-157,
Organism=Escherichia coli, GI1789760, Length=186, Percent_Identity=28.494623655914, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71992717, Length=570, Percent_Identity=35.6140350877193, Blast_Score=292, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI71992710, Length=570, Percent_Identity=35.6140350877193, Blast_Score=292, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI133901714, Length=570, Percent_Identity=35.6140350877193, Blast_Score=292, Evalue=4e-79,
Organism=Saccharomyces cerevisiae, GI6323873, Length=519, Percent_Identity=52.0231213872832, Blast_Score=508, Evalue=1e-144,
Organism=Drosophila melanogaster, GI281365319, Length=548, Percent_Identity=36.4963503649635, Blast_Score=298, Evalue=7e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GUAA_STRP2 (Q04JQ8)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816740.1
- ProteinModelPortal:   Q04JQ8
- SMR:   Q04JQ8
- STRING:   Q04JQ8
- EnsemblBacteria:   EBSTRT00000020035
- GeneID:   4442944
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_1274
- eggNOG:   COG0519
- GeneTree:   EBGT00050000027163
- HOGENOM:   HBG391775
- OMA:   PSTIEPC
- ProtClustDB:   PRK00074
- HAMAP:   MF_00344
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR022310
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR00884
- TIGRFAMs:   TIGR00888

Pfam domain/function: PF00117 GATase; PF00958 GMP_synt_C; PF02540 NAD_synthase

EC number: =6.3.5.2

Molecular weight: Translated: 57473; Mature: 57342

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 90-90 ACT_SITE 179-179 ACT_SITE 181-181

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHKISAAEVREVNPVGIIL
CCCCCHHHHHHCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEE
SGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGGKVVPAGDAGNREYGQSTL
ECCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHCCCHH
THTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSADCPYAAIENPDKHIYGIQFHP
HCCCHHHHCCCCCCCEEEEECCCHHHHCCHHHHHCCCCCCCCCHHCCCCCCEEEEEEECC
EVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKKIRETVGDKRVLLGLSGGVDSSV
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHH
VGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLGGKFGLNIVKADAAKRFLDKLAGVS
HHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCEEEEHHHHHHHHHHHCCCC
DPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGTLYTDVIESGTDTAQTIKSHHNVGGLP
CHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCCCCC
EDMQFELIEPLNTLYKDEVRALGTELGMPDHIVWRQPFPGPGLAIRVMGEITEEKLETVR
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEEEHHHHHHHHHHHHH
ESDAILREEIAKAGLDRDIWQYFTVNTGVRSVGVMGDGRTYDYTIAIRAITSIDGMTADF
HHHHHHHHHHHHCCCCHHHHHHHEECCCCCEEEEECCCCEEEEEEEEEEEHHCCCCCCHH
AKIPWEVLQKISVRIVNEVDHVNRIVYDITSKPPATVEWE
HHCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCC
>Mature Secondary Structure 
SNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHKISAAEVREVNPVGIIL
CCCCHHHHHHCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEE
SGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGGKVVPAGDAGNREYGQSTL
ECCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHCCCHH
THTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSADCPYAAIENPDKHIYGIQFHP
HCCCHHHHCCCCCCCEEEEECCCHHHHCCHHHHHCCCCCCCCCHHCCCCCCEEEEEEECC
EVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKKIRETVGDKRVLLGLSGGVDSSV
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHH
VGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLGGKFGLNIVKADAAKRFLDKLAGVS
HHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCEEEEHHHHHHHHHHHCCCC
DPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGTLYTDVIESGTDTAQTIKSHHNVGGLP
CHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCCCCC
EDMQFELIEPLNTLYKDEVRALGTELGMPDHIVWRQPFPGPGLAIRVMGEITEEKLETVR
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEEEHHHHHHHHHHHHH
ESDAILREEIAKAGLDRDIWQYFTVNTGVRSVGVMGDGRTYDYTIAIRAITSIDGMTADF
HHHHHHHHHHHHCCCCHHHHHHHEECCCCCEEEEECCCCEEEEEEEEEEEHHCCCCCCHH
AKIPWEVLQKISVRIVNEVDHVNRIVYDITSKPPATVEWE
HHCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA