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The map label for this gene is gatA

Identifier: 116516449

GI number: 116516449

Start: 397910

End: 399376

Strand: Reverse

Name: gatA

Synonym: SPD_0397

Alternate gene names: 116516449

Gene position: 399376-397910 (Counterclockwise)

Preceding gene: 116515845

Following gene: 116515626

Centisome position: 19.52

GC content: 45.33

Gene sequence:

>1467_bases
ATGACTTTTAACAATAAAACTATTGAAGAGTTGCACAATCTCCTTGTCTCTAAGGAAATTTCTGCAACAGAATTGACCCA
AGCAACACTTGAAAATATCAAGTCTCGTGAGGAAGCCCTCAATTCATTTGTCACCATCGCTGAGGAGCAAGCTCTTGTTC
AAGCTAAAGCCATTGATGAAGCTGGAATTGATGCTGACAATGTCCTTTCAGGAATTCCACTTGCTGTTAAGGATAACATC
TCTACAGACGGTATTCTCACAACTGCTGCCTCAAAAATGCTCTACAACTATGAGCCAATCTTTGATGCGACAGCTGTTGC
CAATGCAAAAACCAAGGGCATGATTGTCGTTGGAAAGACCAACATGGACGAATTTGCTATGGGTGGTTCCGGTGAAACTT
CACACTACGGAGCAACTAAAAACGCTTGGGACCACAGCAAGGTTCCTGGTGGGTCATCAAGTGGTTCTGCCGCAGCTGTA
GCCTCAGGACAAGTTCGCTTGTCACTTGGTTCTGATACTGGTGGTTCCATCCGCCAACCTGCTGCCTTCAACGGAATCGT
TGGTCTCAAACCAACCTACGGAACAGTTTCACGTTTCGGTCTCATTGCCTTTGGTAGCTCATTAGACCAGATTGGACCTT
TTGCTCCTACTGTTAAGGAAAATGCCCTCTTGCTCAACGCTATTGCCAGCGAAGATGCTAAAGACTCTACTTCTGCTCCT
GTCCGCATCGCCGACTTTACTTCAAAAATCGGCCAAGACATTAAGGGCATGAAAATTGCTTTGCCTAAGGAATACCTAGG
CGAAGGAATTGATCCAGAGGTTAAGGAAACAATCTTAAACGCGGCCAAACACTTTGAAAAATTGGGTGCTATCGTCGAAG
AAGTCAGCCTTCCTCACTCTAAATACGGTGTTGCCGTTTATTACATCATCGCTTCATCAGAAGCTTCATCAAACTTGCAA
CGTTTTGACGGTATCCGTTACGGCTACCGCGCAGAAGATGCGACTAATCTTGATGAAATCTATGTAAACAGCCGTAGCCA
AGGTTTTGGTGAAGAAGTGAAGCGCCGTATCATGCTGGGTACTTTCAGTCTTTCATCAGGTTACTACGATGCCTACTACA
AGAAGGCTGGACAGGTCCGTACACTTATCATTCAAGATTTCGAAAAAGTCTTCGCGGATTACGATTTGATTTTGGGTCCA
ACTGCTCCAAGTGTTGCCTATGACTTGGATTCTCTCAACCATGACCCAGTTGCCATGTACTTAGCCGACCTCTTGACCAT
ACCTGTAAACTTGGCAGGACTGCCTGGAATTTCGATTCCTGCTGGATTCTCTCAAGGTCTACCTGTCGGTCTGCAATTGA
TTGGTCCCAAGCACTCTGAGGAAACCATTTACCAAGTTGCTGCTGCCTTTGAAGCAACAACAGGCTACCACAAACAACAA
CCCGTGATTTTTGGAGGTGACAACTAA

Upstream 100 bases:

>100_bases
AAGAAGGAACAGACCGTGATCGCTTGTTTAAAAACGTACCTGAACAAGACAACTACTATATCAAGGTGCCAGCTATCCTA
GACGATGGAGGAGATGCCTG

Downstream 100 bases:

>100_bases
TGAACTTTGAAACAGTCATTGGACTTGAAGTCCACGTAGAGCTCAACACCAATTCAAAAATCTTCTCACCTACTTCTGCC
CACTTCGGAAATGATCAAAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFVTIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNI
STDGILTTAASKMLYNYEPIFDATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTVKENALLLNAIASEDAKDSTSAP
VRIADFTSKIGQDIKGMKIALPKEYLGEGIDPEVKETILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQ
RFDGIRYGYRAEDATNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFADYDLILGP
TAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVGLQLIGPKHSEETIYQVAAAFEATTGYHKQQ
PVIFGGDN

Sequences:

>Translated_488_residues
MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFVTIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNI
STDGILTTAASKMLYNYEPIFDATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTVKENALLLNAIASEDAKDSTSAP
VRIADFTSKIGQDIKGMKIALPKEYLGEGIDPEVKETILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQ
RFDGIRYGYRAEDATNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFADYDLILGP
TAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVGLQLIGPKHSEETIYQVAAAFEATTGYHKQQ
PVIFGGDN
>Mature_487_residues
TFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFVTIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNIS
TDGILTTAASKMLYNYEPIFDATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAVA
SGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTVKENALLLNAIASEDAKDSTSAPV
RIADFTSKIGQDIKGMKIALPKEYLGEGIDPEVKETILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQR
FDGIRYGYRAEDATNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFADYDLILGPT
APSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVGLQLIGPKHSEETIYQVAAAFEATTGYHKQQP
VIFGGDN

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=506, Percent_Identity=38.5375494071146, Blast_Score=310, Evalue=2e-84,
Organism=Homo sapiens, GI195972892, Length=491, Percent_Identity=25.4582484725051, Blast_Score=125, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17543272, Length=452, Percent_Identity=36.283185840708, Blast_Score=264, Evalue=6e-71,
Organism=Caenorhabditis elegans, GI17537465, Length=479, Percent_Identity=24.6346555323591, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17556264, Length=182, Percent_Identity=32.4175824175824, Blast_Score=89, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17538252, Length=321, Percent_Identity=24.9221183800623, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=188, Percent_Identity=31.9148936170213, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=151, Percent_Identity=34.4370860927152, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556278, Length=151, Percent_Identity=34.4370860927152, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=458, Percent_Identity=32.9694323144105, Blast_Score=231, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6319685, Length=436, Percent_Identity=30.5045871559633, Blast_Score=142, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6320448, Length=193, Percent_Identity=30.0518134715026, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24648113, Length=490, Percent_Identity=41.2244897959184, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI24652985, Length=487, Percent_Identity=26.4887063655031, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI19922090, Length=487, Percent_Identity=26.4887063655031, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24652981, Length=487, Percent_Identity=26.4887063655031, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24652983, Length=487, Percent_Identity=26.4887063655031, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI161078093, Length=510, Percent_Identity=24.9019607843137, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24644968, Length=511, Percent_Identity=24.0704500978474, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI45550774, Length=522, Percent_Identity=24.1379310344828, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648435, Length=522, Percent_Identity=24.1379310344828, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648437, Length=522, Percent_Identity=24.1379310344828, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21356731, Length=506, Percent_Identity=23.7154150197628, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24648441, Length=454, Percent_Identity=24.2290748898678, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648439, Length=454, Percent_Identity=24.2290748898678, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_STRP2 (Q04M41)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_815907.1
- ProteinModelPortal:   Q04M41
- SMR:   Q04M41
- STRING:   Q04M41
- PhosSite:   Q04M41
- EnsemblBacteria:   EBSTRT00000020450
- GeneID:   4442568
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_0397
- eggNOG:   COG0154
- GeneTree:   EBGT00050000026783
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- ProtClustDB:   PRK00012
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 52005; Mature: 51874

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 77-77 ACT_SITE 152-152 ACT_SITE 176-176

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFVTIAEEQALVQAKAIDE
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFDATAVANAKTKGMIVVGKT
CCCCHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEECC
NMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAVASGQVRLSLGSDTGGSIRQP
CHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCCCCCCCCC
AAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTVKENALLLNAIASEDAKDSTSAP
CCCCCCEECCCCCCHHHHHHHHHCCCCHHHCCCCCCCCCCCCEEEEHHHCCCCCCCCCCC
VRIADFTSKIGQDIKGMKIALPKEYLGEGIDPEVKETILNAAKHFEKLGAIVEEVSLPHS
EEEHHHHHHHCCCCCCCEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDATNLDEIYVNSRSQGFGEEVKRRIMLG
CCCEEEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCHHEEECCCCCCCHHHHHHHHEEE
TFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFADYDLILGPTAPSVAYDLDSLNHDPVAMY
EEECCCCCHHHHHHHCCCEEHHHHHHHHHHHCCCCEEECCCCCCHHHHHHCCCCCHHHHH
LADLLTIPVNLAGLPGISIPAGFSQGLPVGLQLIGPKHSEETIYQVAAAFEATTGYHKQQ
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCC
PVIFGGDN
CEEECCCC
>Mature Secondary Structure 
TFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFVTIAEEQALVQAKAIDE
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFDATAVANAKTKGMIVVGKT
CCCCHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEECC
NMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAVASGQVRLSLGSDTGGSIRQP
CHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCCCCCCCCC
AAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTVKENALLLNAIASEDAKDSTSAP
CCCCCCEECCCCCCHHHHHHHHHCCCCHHHCCCCCCCCCCCCEEEEHHHCCCCCCCCCCC
VRIADFTSKIGQDIKGMKIALPKEYLGEGIDPEVKETILNAAKHFEKLGAIVEEVSLPHS
EEEHHHHHHHCCCCCCCEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDATNLDEIYVNSRSQGFGEEVKRRIMLG
CCCEEEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCHHEEECCCCCCCHHHHHHHHEEE
TFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFADYDLILGPTAPSVAYDLDSLNHDPVAMY
EEECCCCCHHHHHHHCCCEEHHHHHHHHHHHCCCCEEECCCCCCHHHHHHCCCCCHHHHH
LADLLTIPVNLAGLPGISIPAGFSQGLPVGLQLIGPKHSEETIYQVAAAFEATTGYHKQQ
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCC
PVIFGGDN
CEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA