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The map label for this gene is potD [H]

Identifier: 116516276

GI number: 116516276

Start: 1250318

End: 1251388

Strand: Reverse

Name: potD [H]

Synonym: SPD_1218

Alternate gene names: 116516276

Gene position: 1251388-1250318 (Counterclockwise)

Preceding gene: 116517092

Following gene: 116515414

Centisome position: 61.16

GC content: 41.64

Gene sequence:

>1071_bases
ATGAAAAAAATCTATTCATTTTTAGCAGGAATTGCAGCGATTATCCTTGTCTTGTGGGGAATTGCGACTCATTTAGATAG
TAAAATCAATAGTCGAGATAGTCAAAAATTGGTTATCTATAACTGGGGAGACTATATCGATCCTGAACTCTTGACTCAGT
TTACAGAAGAAACAGGAATTCAAGTTCAGTACGAGGCTTTTGACTCCAACGAAGCCATGTACACTAAGATAAAGCAGGGT
GGAACGACCTACGATATTGCCATTCCAAGTGAATACATGATTAACAAGATGAAGGACGAAGACCTCTTGGTTCCGCTTGA
TTATTCAAAAATTGAAGGAATCGAAAATATCGGACCAGAGTTTCTCAACCAGTCCTTTGACCCAGGTAATAAATTCTCCA
TCCCTTACTTCTGGGGAACCTTAGGAATTGTCTACAACGAAACCATGGTAGATGAAGCGCCTGAGCATTGGGATGACCTT
TGGAAGCTGGAGTATAAGAATTCTATCATGCTTTTTGATGGGGCGCGTGAGGTGCTGGGACTAGGACTCAATTCCCTCGG
CTACAGCCTCAACTCCAAGGATCCGCAGCAGTTGGAAGAGACAGTGGATAAGCTCTACAAACTGACTCCAAATATCAAGG
CTATCGTTGCGGACGAGATGAAGGGCTACATGATTCAGAATAATGTTGCAATCGGCGTGACCTTCTCTGGTGAAGCCAGC
CAAATGTTAGAAAAAAATGAAAATCTACGTTATGTGGTACCGACAGAGGCCAGCAATCTTTGGTTTGACAATATGGTCAT
TCCCAAAACAGTTAAAAATCAAGACTCAGCCTATGCCTTTATCAACTTTATGTTGAAACCTGAAAATGCTCTCCAAAATG
CGGAGTATGTCGGCTATTCAACACCAAACCTACCAGCGAAGGAATTGCTCCCAGAGGAAACAAAGGAAGATAAGGCCTTC
TATCCCGATGTTGAAACCATGAAACACCTAGAAGTTTATGAGAAATTTGACCATAAATGGACAGGGAAATATAGCGACCT
CTTCCTACAGTTTAAAATGTATCGGAAGTAG

Upstream 100 bases:

>100_bases
TTTCGCTAGAAATCAATGCCCTTTCTGCTCTAGTCTTTCTCTTTAGTATTATCCTAGTTGTAGGTTATTACTTTATCTCT
CGTGAGAAGGAGGAGCAAGC

Downstream 100 bases:

>100_bases
GAGATAAACGTAAGAAGACGAATCAGTCAAGCTGGTTCGTTTTTAAGGAATAGAAGTCAGTTTTTAGAGCCCTATCTTTT
TCTATTCTAGTTGTTTATTT

Product: spermidine/putrescine ABC transporter spermidine/putrescine-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGDYIDPELLTQFTEETGIQVQYEAFDSNEAMYTKIKQG
GTTYDIAIPSEYMINKMKDEDLLVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
WKLEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDPQQLEETVDKLYKLTPNIKAIVADEMKGYMIQNNVAIGVTFSGEAS
QMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQDSAYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAF
YPDVETMKHLEVYEKFDHKWTGKYSDLFLQFKMYRK

Sequences:

>Translated_356_residues
MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGDYIDPELLTQFTEETGIQVQYEAFDSNEAMYTKIKQG
GTTYDIAIPSEYMINKMKDEDLLVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
WKLEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDPQQLEETVDKLYKLTPNIKAIVADEMKGYMIQNNVAIGVTFSGEAS
QMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQDSAYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAF
YPDVETMKHLEVYEKFDHKWTGKYSDLFLQFKMYRK
>Mature_356_residues
MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGDYIDPELLTQFTEETGIQVQYEAFDSNEAMYTKIKQG
GTTYDIAIPSEYMINKMKDEDLLVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
WKLEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDPQQLEETVDKLYKLTPNIKAIVADEMKGYMIQNNVAIGVTFSGEAS
QMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQDSAYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAF
YPDVETMKHLEVYEKFDHKWTGKYSDLFLQFKMYRK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=307, Percent_Identity=41.6938110749186, Blast_Score=252, Evalue=2e-68,
Organism=Escherichia coli, GI1787078, Length=344, Percent_Identity=30.5232558139535, Blast_Score=169, Evalue=3e-43,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40982; Mature: 40982

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGDYIDPELLTQFTEETGI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCC
QVQYEAFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDLLVPLDYSKIEGIENIGPE
EEEEEEECCCCCEEEEECCCCCEEEEECCHHHHHHHCCCCCEEEECCHHHHCCHHHCCHH
FLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDLWKLEYKNSIMLFDGAREVLG
HHCCCCCCCCCEECCHHHHHHHHEECCHHHHCCHHHHHHHEEEEECCEEEEECCHHHHHH
LGLNSLGYSLNSKDPQQLEETVDKLYKLTPNIKAIVADEMKGYMIQNNVAIGVTFSGEAS
CCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCEEEEEEECCCHH
QMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQDSAYAFINFMLKPENALQNAEYVGYS
HHHHCCCCCEEEECCCCCCEEECCEECCCHHCCCCCEEEEEEEEECCCHHHCCCCEECCC
TPNLPAKELLPEETKEDKAFYPDVETMKHLEVYEKFDHKWTGKYSDLFLQFKMYRK
CCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHEEEEEECCC
>Mature Secondary Structure
MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGDYIDPELLTQFTEETGI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCC
QVQYEAFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDLLVPLDYSKIEGIENIGPE
EEEEEEECCCCCEEEEECCCCCEEEEECCHHHHHHHCCCCCEEEECCHHHHCCHHHCCHH
FLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDLWKLEYKNSIMLFDGAREVLG
HHCCCCCCCCCEECCHHHHHHHHEECCHHHHCCHHHHHHHEEEEECCEEEEECCHHHHHH
LGLNSLGYSLNSKDPQQLEETVDKLYKLTPNIKAIVADEMKGYMIQNNVAIGVTFSGEAS
CCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCEEEEEEECCCHH
QMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQDSAYAFINFMLKPENALQNAEYVGYS
HHHHCCCCCEEEECCCCCCEEECCEECCCHHCCCCCEEEEEEEEECCCHHHCCCCEECCC
TPNLPAKELLPEETKEDKAFYPDVETMKHLEVYEKFDHKWTGKYSDLFLQFKMYRK
CCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]