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The map label for this gene is tyrS [H]

Identifier: 116516055

GI number: 116516055

Start: 1896175

End: 1897431

Strand: Direct

Name: tyrS [H]

Synonym: SPD_1926

Alternate gene names: 116516055

Gene position: 1896175-1897431 (Clockwise)

Preceding gene: 116516192

Following gene: 116516682

Centisome position: 92.67

GC content: 43.75

Gene sequence:

>1257_bases
ATGCACATTTTTGATGAGCTAAAAGAGCGTGGTTTGATATTTCAAACGACTGATGAAGAAGCTTTGCGTAAAGCCCTAGA
AGAAGGTCAAGTTTCTTATTATACTGGCTACGATCCAACTGCTGACAGCCTTCACCTAGGCCACCTTGTCGCAATCTTGA
CAAGTCGTCGCTTGCAACTAGCAGGTCACAAACCTTATGCGCTCGTTGGCGGTGCTACAGGTCTCATCGGAGATCCGTCC
TTCAAAGATGCTGAACGTAGTCTCCAAACAAAAGACACAGTAGATGGCTGGGTCAAGTCTATCCAAGGACAACTTTCTCG
TTTTCTTGACTTTGAAAATGGCGAAAACAAGGCTGTCATGGTCAACAACTACGACTGGTTTGGCAGCATCAGCTTCATTG
ACTTCCTCCGTGATATTGGAAAATACTTCACGGTCAACTACATGATGAGTAAGGAATCTGTTAAAAAACGGATCGAAACA
GGAATTTCTTACACTGAGTTCGCTTACCAAATCATGCAAGGGTATGACTTCTTCGTCCTTAACCAAGACCATAATGTCAC
TCTTCAAATCGGTGGTTCTGACCAGTGGGGAAATATGACAGCTGGTACCGAATTGCTTCGTCGTAAGGCGGACAAGACTG
GTCACGTTATCACTGTTCCACTAATCACAGATGCAACTGGTAAGAAATTTGGTAAATCAGAAGGAAATGCCGTCTGGCTC
AATCCCGAAAAGACTTCTCCATACGAAATGTACCAATTCTGGATGAACGTGATGGACGCTGACGCTGTTCGCTTCTTGAA
AATCTTTACTTTCTTGTCACTTGATGAGATTGAAGATATTCGTAAACAATTTGAAGCAGCGCCACACGAACGCTTGGCTC
AAAAAGTCTTGGCTCGTGAAGTTGTTACACTTGTTCACGGAGAAGAAGCCTACAAAGAAGCACTTAACATCACTGAGCAA
CTCTTTGCAGGAAACATCAAAAACCTTTCTGTCAAAGAGCTCAAACAAGGACTTCGTGGTGTGCCAAACTACCAAGTACA
GGCAGACGAAAACAACAATATCGTGGAACTGCTCGTCTCATCTGGTATAGTTAACTCAAAACGCCAAGCCCGTGAAGACG
TCCAAAACGGAGCCATCTACGTAAACGGCGACCGCATCCAAGAGCTTGACTATGTCTTGAGTGACGCTGATAAGTTAGAG
AATGAACTGACTGTTATCCGTCGTGGGAAGAAAAAATACTTTGTATTGACTTACTAA

Upstream 100 bases:

>100_bases
TTCAATAAAATAGCCACTTTCTTCCCTATTCTGCTAGGCTATTGCCCAAGTTTGTGATACAATAGGTAGAAACAATAATT
TTAAAAAGGAGAAAAAACAC

Downstream 100 bases:

>100_bases
ACTATTCAACATTTATCTATAAACAAAGGAGTTAACCTCGAGAAAGGTAACTCCTTTTTGCTGTTAATAACTCTCATCTA
TCTATTTTTAATAGACAGGC

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSLHLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPS
FKDAERSLQTKDTVDGWVKSIQGQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVITVPLITDATGKKFGKSEGNAVWL
NPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDEIEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQ
LFAGNIKNLSVKELKQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQELDYVLSDADKLE
NELTVIRRGKKKYFVLTY

Sequences:

>Translated_418_residues
MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSLHLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPS
FKDAERSLQTKDTVDGWVKSIQGQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVITVPLITDATGKKFGKSEGNAVWL
NPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDEIEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQ
LFAGNIKNLSVKELKQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQELDYVLSDADKLE
NELTVIRRGKKKYFVLTY
>Mature_418_residues
MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSLHLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPS
FKDAERSLQTKDTVDGWVKSIQGQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVITVPLITDATGKKFGKSEGNAVWL
NPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDEIEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQ
LFAGNIKNLSVKELKQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQELDYVLSDADKLE
NELTVIRRGKKKYFVLTY

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=432, Percent_Identity=35.1851851851852, Blast_Score=241, Evalue=1e-63,
Organism=Escherichia coli, GI1787925, Length=421, Percent_Identity=51.0688836104513, Blast_Score=445, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI25145802, Length=388, Percent_Identity=34.2783505154639, Blast_Score=226, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI25145805, Length=318, Percent_Identity=33.0188679245283, Blast_Score=173, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6325160, Length=440, Percent_Identity=35.9090909090909, Blast_Score=277, Evalue=3e-75,
Organism=Drosophila melanogaster, GI20130383, Length=436, Percent_Identity=38.302752293578, Blast_Score=316, Evalue=2e-86,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 47481; Mature: 47481

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSLHLGHLVAILTSRRLQL
CCCHHHHHHCCCEEEECCHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHCHHEE
AGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQGQLSRFLDFENGENKAVM
CCCCCEEEECCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
VNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIETGISYTEFAYQIMQGYDFFVL
EECCCCCCCHHHHHHHHHHHHHEEEHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCEEEE
NQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVITVPLITDATGKKFGKSEGNAVWL
ECCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCEEEE
NPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDEIEDIRKQFEAAPHERLAQKVLARE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHH
VVTLVHGEEAYKEALNITEQLFAGNIKNLSVKELKQGLRGVPNYQVQADENNNIVELLVS
HHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCEEEHHHH
SGIVNSKRQAREDVQNGAIYVNGDRIQELDYVLSDADKLENELTVIRRGKKKYFVLTY
CCCCCCHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
>Mature Secondary Structure
MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSLHLGHLVAILTSRRLQL
CCCHHHHHHCCCEEEECCHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHCHHEE
AGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQGQLSRFLDFENGENKAVM
CCCCCEEEECCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
VNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIETGISYTEFAYQIMQGYDFFVL
EECCCCCCCHHHHHHHHHHHHHEEEHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCEEEE
NQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVITVPLITDATGKKFGKSEGNAVWL
ECCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCEEEE
NPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDEIEDIRKQFEAAPHERLAQKVLARE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHH
VVTLVHGEEAYKEALNITEQLFAGNIKNLSVKELKQGLRGVPNYQVQADENNNIVELLVS
HHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCEEEHHHH
SGIVNSKRQAREDVQNGAIYVNGDRIQELDYVLSDADKLENELTVIRRGKKKYFVLTY
CCCCCCHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA