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The map label for this gene is ansA [H]

Identifier: 116515808

GI number: 116515808

Start: 1789645

End: 1790607

Strand: Direct

Name: ansA [H]

Synonym: SPD_1796

Alternate gene names: 116515808

Gene position: 1789645-1790607 (Clockwise)

Preceding gene: 116515416

Following gene: 116515727

Centisome position: 87.47

GC content: 45.79

Gene sequence:

>963_bases
ATGCCTAAGAAAATCCTTGTTTTACATACGGGTGGAACTATTTCCATGCAGGCCGATGCTTCTGGCGCTGTTGTGACGAG
TTCAGATAATCCCATGAACCATGTGTCCAACCCACTTGAAGGAATCCAAGTCCACGCCTTGGACTTTTTTAACCTTCCAA
GTCCCCATATCAAACCCAAACATATGCTGGTCCTCTACCAGAAAATTAAAGAGGAAGCAGATAACTACGATGGAGTGGTG
ATCACACACGGAACCGATACTTTAGAGGAAACAGCCTATTTCCTTGATACCATGGAAGTTCCCCATATGCCTATCGTTCT
AACAGGAGCCATGCGTAGCTCCAATGAGCTCGGTAGTGATGGTGTTTATAATTACCTAAGTGCTTTACGAGTGGCCAGCG
ATGACAGGGCTGCTGACAAAGGAGTTTTGGTCGTTATGAACGATGAAATCCACGCTGCCAAGTATGTCACCAAAACACAT
ACGACTAATGTCAGCACCTTCCAGACTCCAACACATGGCCCCCTCGGTCTCATCATGAAACAGGAAATCCTCTACTTCAA
AACAGCTGAACCTCGTGTTCGCTTTGACCTTGATCACATACAAGGTTTAGTCCCTATCATCTCGGCTTATGCTGGTATGA
CAGATGAGCTGATTGATATGCTGGATTTAGAACACTTGGACGGTTTGATTATCCAAGCCTTCGGAGCTGGTAATATTCCC
AAAGAAACGGCTCAAAAATTAGAAAGCCTTCTGCAAAAAGGAATCCCAGTCGCTCTGGTATCACGATGCTTTAACGGTAT
TGCCGAGCCTGTTTATGCCTACCAGGGTGGGGGCGTACAGTTGCAAAAAGCAGGCGTTTTCTTTGTTAAAGAACTCAACG
CCCAAAAAGCTCGCTTGAAACTCCTCATCGCCCTCAATGCCGGACTAACAGGACAGGCTTTGAAAGACTATATGGAAGGC
TAA

Upstream 100 bases:

>100_bases
CCATCGATATCAAAAAAAACAGCTTTGACTTCCATCCTATTCCAATCTCCCCTTTTGTGATACAATGATTATACCACATT
TCAGAAAGAGTTAGTAAATC

Downstream 100 bases:

>100_bases
TACTCTTCGAAAATCTCTGCAAACCACGTCAGCGTCGCCTTACCGTATGTATGGTTACTGACTTCGTCAGTTTCATCTAC
AACCTCAAAAACATGTTTTG

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQVHALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVV
ITHGTDTLEETAYFLDTMEVPHMPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELIDMLDLEHLDGLIIQAFGAGNIP
KETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQGGGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG

Sequences:

>Translated_320_residues
MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQVHALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVV
ITHGTDTLEETAYFLDTMEVPHMPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELIDMLDLEHLDGLIIQAFGAGNIP
KETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQGGGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG
>Mature_319_residues
PKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQVHALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVI
THGTDTLEETAYFLDTMEVPHMPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTHT
TNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELIDMLDLEHLDGLIIQAFGAGNIPK
ETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQGGGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=283, Percent_Identity=36.0424028268551, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1788065, Length=333, Percent_Identity=27.6276276276276, Blast_Score=89, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17558278, Length=342, Percent_Identity=26.0233918128655, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI193207372, Length=342, Percent_Identity=26.0233918128655, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI193207374, Length=342, Percent_Identity=26.0233918128655, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17558280, Length=342, Percent_Identity=26.0233918128655, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320527, Length=331, Percent_Identity=27.7945619335347, Blast_Score=126, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6323189, Length=339, Percent_Identity=27.4336283185841, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6323187, Length=339, Percent_Identity=27.4336283185841, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6323186, Length=339, Percent_Identity=27.4336283185841, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6323184, Length=339, Percent_Identity=27.4336283185841, Blast_Score=117, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24645530, Length=256, Percent_Identity=27.734375, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24639609, Length=215, Percent_Identity=25.1162790697674, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 34870; Mature: 34738

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS01076 ACETATE_KINASE_2 ; PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQVHALDFFNLPSPHIKPK
CCCEEEEEECCCEEEEECCCCCEEEECCCCCHHHHCCCCCCEEEEEEEEECCCCCCCCCH
HMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPHMPIVLTGAMRSSNELGSD
HHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCC
GVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTHTTNVSTFQTPTHGPLGLIMK
HHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHEEEECCCCCCEEECCCCCCCHHHHHH
QEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELIDMLDLEHLDGLIIQAFGAGNIP
HEEEEEECCCCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEEECCCCCCC
KETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQGGGVQLQKAGVFFVKELNAQKARLK
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEECCCCEEEEECCEEEEECCCCCCEEEE
LLIALNAGLTGQALKDYMEG
EEEEECCCCCHHHHHHHHCC
>Mature Secondary Structure 
PKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQVHALDFFNLPSPHIKPK
CCEEEEEECCCEEEEECCCCCEEEECCCCCHHHHCCCCCCEEEEEEEEECCCCCCCCCH
HMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPHMPIVLTGAMRSSNELGSD
HHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCC
GVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTHTTNVSTFQTPTHGPLGLIMK
HHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHEEEECCCCCCEEECCCCCCCHHHHHH
QEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELIDMLDLEHLDGLIIQAFGAGNIP
HEEEEEECCCCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEEECCCCCCC
KETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQGGGVQLQKAGVFFVKELNAQKARLK
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEECCCCEEEEECCEEEEECCCCCCEEEE
LLIALNAGLTGQALKDYMEG
EEEEECCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]