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The map label for this gene is yhcT [H]

Identifier: 116515727

GI number: 116515727

Start: 1806543

End: 1807418

Strand: Direct

Name: yhcT [H]

Synonym: SPD_1822

Alternate gene names: 116515727

Gene position: 1806543-1807418 (Clockwise)

Preceding gene: 116515808

Following gene: 116517036

Centisome position: 88.29

GC content: 39.61

Gene sequence:

>876_bases
ATGCAATTCACATTTACATTACCCGCCTCTCTACCTCAAATGACGGTAAAGCAATTACTTGAGGAACAACTCCTCATCCC
TAGAAAAATCCGTCATTTTTTGAGAATCAAGAAACATATTTTGATAAATCAAGAAGAAGTCCGCTGGAACGAAATCGTAA
ATCCTGGAGATGTTTGCCAGTTGACTTTTGACGAGGAAGATTATCCCCAAAAGACGATCCCTTGGGGCAACCCAGACTTA
GTGCAGGAAGTTTATCAAGATCAACACTTGATTATTGTAAACAAACCAGAGGGGATGAAAACGCATGGTAATCAACCAAA
CGAAATTGCCCTTCTTAACCATGTCAGTACCTATGTTGGCCAAACCTGCTATGTCGTTCATCGTCTGGACATGGAAACCA
GTGGCTTAGTTCTCTTTGCCAAAAATCCTTTTATCCTGCCCATTCTCAATCGCTTATTGGAGAAAAAAGAGATTTCTAGA
GAATATTGGGCTCTAGTTGATGGAAATATCAACAGAAAAGAACTTGTTTTCAGAGACAAAATTGGACGTGATCGCCATGA
TCGTAGAAAAAGAATAGTTGATGCAAAAAATGGGAAATATGCTGAAACGCATGTAAGCAGATTAAAGCAATTCTCAAACA
AGACTTCCTTGGCTCATTGCAAGCTAAAGACAGGGCGAACCCATCAGATTCGTGTGCACCTTTTGCATCATAATCTTCCT
ATCCTGGGAGACCCTCTCTATAATAGTAAATCAAAGACAAGCCGGCTTATGCTTCATGCCTTCCGACTTTCCTTTACCCA
CCCACTTACTTTAGAGAGGCTAACTTTCACTACCCTTTCAAATACATTTGAAAAAGAATTAAAAAAGAATGGATGA

Upstream 100 bases:

>100_bases
TTATCTAATTTCATGCGTTTATTTTATCATCTTCATCATAGGAAGACAAGAATTTAGCTATTTCCTATCCAAATAGGGCT
TTTTTTGTTACAATATCTGT

Downstream 100 bases:

>100_bases
TCGTGTCATCCATTTTTCCATATAAAAAAGCAAGACCACAAAGCCTTGCTTTCTATCAACTCAAGAATTATTTAGCAATC
TTTGCGAAGTATTCAAGAGT

Product: ribosomal large subunit pseudouridine synthase, RluD subfamily protein

Products: NA

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MQFTFTLPASLPQMTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVRWNEIVNPGDVCQLTFDEEDYPQKTIPWGNPDL
VQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHVSTYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISR
EYWALVDGNINRKELVFRDKIGRDRHDRRKRIVDAKNGKYAETHVSRLKQFSNKTSLAHCKLKTGRTHQIRVHLLHHNLP
ILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLERLTFTTLSNTFEKELKKNG

Sequences:

>Translated_291_residues
MQFTFTLPASLPQMTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVRWNEIVNPGDVCQLTFDEEDYPQKTIPWGNPDL
VQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHVSTYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISR
EYWALVDGNINRKELVFRDKIGRDRHDRRKRIVDAKNGKYAETHVSRLKQFSNKTSLAHCKLKTGRTHQIRVHLLHHNLP
ILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLERLTFTTLSNTFEKELKKNG
>Mature_291_residues
MQFTFTLPASLPQMTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVRWNEIVNPGDVCQLTFDEEDYPQKTIPWGNPDL
VQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHVSTYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISR
EYWALVDGNINRKELVFRDKIGRDRHDRRKRIVDAKNGKYAETHVSRLKQFSNKTSLAHCKLKTGRTHQIRVHLLHHNLP
ILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLERLTFTTLSNTFEKELKKNG

Specific function: Unknown

COG id: COG0564

COG function: function code J; Pseudouridylate synthases, 23S RNA-specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI23308689, Length=176, Percent_Identity=32.3863636363636, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI221316663, Length=191, Percent_Identity=24.0837696335079, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI17158025, Length=202, Percent_Identity=26.7326732673267, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1789155, Length=235, Percent_Identity=29.3617021276596, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1786244, Length=203, Percent_Identity=32.512315270936, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788946, Length=211, Percent_Identity=33.6492890995261, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787327, Length=202, Percent_Identity=32.1782178217822, Blast_Score=92, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17534965, Length=172, Percent_Identity=34.3023255813954, Blast_Score=71, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6324506, Length=226, Percent_Identity=27.4336283185841, Blast_Score=86, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6320168, Length=215, Percent_Identity=28.3720930232558, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321608, Length=213, Percent_Identity=26.2910798122066, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24583449, Length=174, Percent_Identity=36.7816091954023, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24583447, Length=174, Percent_Identity=36.7816091954023, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24583445, Length=174, Percent_Identity=36.7816091954023, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19921090, Length=174, Percent_Identity=31.0344827586207, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006225
- InterPro:   IPR006224
- InterPro:   IPR006145
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 34092; Mature: 34092

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: PS01129 PSI_RLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFTFTLPASLPQMTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVRWNEIVNPGDVCQ
CEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEE
LTFDEEDYPQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHVSTYVG
EEECCCCCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEHHHHHHHHHC
QTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRKELVFRDK
CEEEEEEEECCCCCCEEEEECCCEEHHHHHHHHHHHHHCCHHEEEECCCCCHHHHHHHHH
IGRDRHDRRKRIVDAKNGKYAETHVSRLKQFSNKTSLAHCKLKTGRTHQIRVHLLHHNLP
CCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEEEECCCC
ILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLERLTFTTLSNTFEKELKKNG
EECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQFTFTLPASLPQMTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVRWNEIVNPGDVCQ
CEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEE
LTFDEEDYPQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHVSTYVG
EEECCCCCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEHHHHHHHHHC
QTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRKELVFRDK
CEEEEEEEECCCCCCEEEEECCCEEHHHHHHHHHHHHHCCHHEEEECCCCCHHHHHHHHH
IGRDRHDRRKRIVDAKNGKYAETHVSRLKQFSNKTSLAHCKLKTGRTHQIRVHLLHHNLP
CCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEEEECCCC
ILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLERLTFTTLSNTFEKELKKNG
EECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]