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The map label for this gene is recU

Identifier: 116515550

GI number: 116515550

Start: 342548

End: 343144

Strand: Reverse

Name: recU

Synonym: SPD_0337

Alternate gene names: 116515550

Gene position: 343144-342548 (Counterclockwise)

Preceding gene: 116516093

Following gene: 116516330

Centisome position: 16.77

GC content: 37.52

Gene sequence:

>597_bases
ATGGTCAACTATCCACATAAAGTTTCATCACAAAAAAGACAAACATCTCTTTCTCAACCCAAAAATTTCGCAAATCGAGG
AATGTCTTTTGAAAAGATGATCAATGCTACCAACGACTACTATTTGTCTCAGGGCTTGGCTGTTATACATAAGAAACCAA
CTCCTATTCAAATCGTACAAGTGGACTATCCACAACGAAGTCGTGCCAAGATTGTTGAAGCCTATTTTCGACAAGCTTCA
ACGACGGACTATTCTGGCGTTTATAATGGATATTACATCGACTTTGAAGTCAAGGAAACAAAACAAAAACGTGCGATTCC
GATGAAAAATTTTCATCCACATCAGATTCAGCATATGGAACAAGTCCTTGCCCAACAAGGAATCTGCTTTGTCCTTCTTC
ACTTTTCTTCTCAGCAAGAAACCTACTTATTGCCGGCATTCGATTTGATTCGCTTCTATCATCAAGATAAGGGACAAAAA
TCAATGCCACTTGAATATATTCGAGAATATGGATATGAAATCAAGGCTGGTGCCTTCCCTCAAATTCCTTATCTCAATGT
TATCAAAGAACATTTATTAGGTGGTAAAACAAGATGA

Upstream 100 bases:

>100_bases
AAAGCAGAATAGCCTAAAACCAAAGCTGTAGCCATTTTTAACCCTTTCTGTGCAAATTATGGTATAATAAAGTGATGTTA
TTGTACCAATAAGGAGAATT

Downstream 100 bases:

>100_bases
ACAAACCAACGATTCTGCGCCTAATCAAGTATCTGAGCATTAGCTTCTTAAGCTTGGTTATCGCAGCCATTGTCTTAGGC
GGAGGAGTTTTTTTCTACTA

Product: Holliday junction-specific endonuclease

Products: NA

Alternate protein names: Recombination protein U homolog

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQGLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQAS
TTDYSGVYNGYYIDFEVKETKQKRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR

Sequences:

>Translated_198_residues
MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQGLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQAS
TTDYSGVYNGYYIDFEVKETKQKRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR
>Mature_198_residues
MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQGLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQAS
TTDYSGVYNGYYIDFEVKETKQKRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR

Specific function: Endonuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves mobile four-strand junctions by introducing symmetrical nicks in paired strands. Promotes annealing of linear ssDNA with homologous dsDNA. Required for DNA repair

COG id: COG3331

COG function: function code R; Penicillin-binding protein-related factor A, putative recombinase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recU family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECU_STRP2 (Q04M97)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_815851.1
- ProteinModelPortal:   Q04M97
- SMR:   Q04M97
- STRING:   Q04M97
- EnsemblBacteria:   EBSTRT00000020591
- GeneID:   4442724
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_0337
- eggNOG:   COG3331
- GeneTree:   EBGT00050000028296
- HOGENOM:   HBG571967
- OMA:   GHYIDFE
- ProtClustDB:   PRK02234
- GO:   GO:0005737
- HAMAP:   MF_00130
- InterPro:   IPR004612
- InterPro:   IPR011335
- PIRSF:   PIRSF037785
- TIGRFAMs:   TIGR00648

Pfam domain/function: PF03838 RecU; SSF52980 Restrict_endonuc_II-like_core

EC number: NA

Molecular weight: Translated: 23131; Mature: 23131

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQGLAVIHKKPTPIQIVQ
CCCCCCHHHHHHHHHHHHCCHHHHHCCCCHHHHHCCCCHHHHHCCEEEEECCCCCEEEEE
VDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQKRAIPMKNFHPHQIQHME
ECCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCHHHHHCCCCCCCCHHHHHHHH
QVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQKSMPLEYIREYGYEIKAGAFP
HHHHHCCCEEEEEEECCCCCEEECHHHHHHHHHHHCCCCCCCCHHHHHHCCCEECCCCCC
QIPYLNVIKEHLLGGKTR
CCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQGLAVIHKKPTPIQIVQ
CCCCCCHHHHHHHHHHHHCCHHHHHCCCCHHHHHCCCCHHHHHCCEEEEECCCCCEEEEE
VDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQKRAIPMKNFHPHQIQHME
ECCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCHHHHHCCCCCCCCHHHHHHHH
QVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQKSMPLEYIREYGYEIKAGAFP
HHHHHCCCEEEEEEECCCCCEEECHHHHHHHHHHHCCCCCCCCHHHHHHCCCEECCCCCC
QIPYLNVIKEHLLGGKTR
CCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA