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The map label for this gene is gmuD [H]

Identifier: 116515373

GI number: 116515373

Start: 1814981

End: 1816390

Strand: Direct

Name: gmuD [H]

Synonym: SPD_1830

Alternate gene names: 116515373

Gene position: 1814981-1816390 (Clockwise)

Preceding gene: 116517036

Following gene: 116515645

Centisome position: 88.7

GC content: 38.58

Gene sequence:

>1410_bases
ATGAAAAAAAAGTTAGTATTTCCTAATCTGTTTTGGTGGGGAGCAGCTTCTAGCGGACCTCAGACAGAAGGTCAATATGG
AAAAGTACATGAAAATGTGATGGACTACTGGTTCAAAACGCATCCAGAAGATTTTTTCGATAATGTCGGACCTCTTGTAG
CCAGTAACTTTTTTCATACTTACACCGAAGATTTCCACTTGATGAAGGAAATTGGAGTTAATTCTTTCCGCACTTCCATC
CAATGGAGTCGACTCATCAAGAATTTAGAGACAGGTGAGCCTGATCCAAAAGGTATTGCTTTCTACAATGCCATCATTGA
AGAAGCTAAAAAGAACCAGATGGATCTTGTGATGAATTTACATCATTTTGATTTACCAGTGGAACTTCTTCAAAAATACG
GTGGTTGGGAAAGCAAACATGTAGTGGAGTTATTCGTGAAGTTTGCCAAGACTGCTTTCACATGCTTTGGAGATAAGGTT
CATTACTGGACAACTTTCAATGAGCCAATGGTCATTCCAGAAGCAGGATACTTATATGCTTTCCATTATCCAAATCTAAA
AGGAAAGGGAAAAGAGGCCGTACAAGTCATCTATAATCTAAACCTTGCTAGTGCAAAAGTGATTCAACTATATCGCTCAT
TAGAACTTGATGGAAAGATTGGGATTATTTTAAACTTGACACCTGCTTATCCAAGAAGTAATTCTCCAGAAGACTTAGAA
GCAAGTCGATTTACAGATGACTTCTTTAACAAAGTCTTCTTGAATCCAGCTGTTAAAGGAACTTTCCCAGAAAGATTGGT
AAAACAGCTAGAGAGAGATGGCGTGTTATGGAGTCATACCGAAAAAGAGCTTCAACTGATGAAATCAAATACGGTTGATT
TTCTTGGAGTAAACTACTACCATCCAAAACGTGTTCAAGCACAAGCAAATCCTGAGGAATATCAGACGCCCTGGATGCCA
GACCAATACTTCAAAGAGTATGAATGGCCGGAGCGTCGCATGAATCCATATCGTGGTTGGGAAATTTTTCCGAAAGCCAT
TTATGATATTGCTATGATTGTGAAGGAAGAATATGGTAATATCCCATGGTTTATCAGTGAAAACGGAATGGGTGTTGAAA
ACGAAGCACGGTTTATCGATGAAAATGGAGTTATCGATGACGTGTATCGTATTGAATTTTATGAAGAACATTTAAGATGG
CTACATAAAGCCATTGAAGAGGGAAGTCACTGTTTTGGATACCACGCTTGGACCGCATTTGATTGCTGGTCTTGGAATAA
TGCATATAAGAATCGTTACGGATTTATCTCCGTTGATTTAGAAACGCAAAAGAGAACCATCAAGAGCTCAGGAAGATGGT
ATCGCAAAGTAAGTGACAATAACGGTTTTGAAGTAGAAATTGAGGAGTAA

Upstream 100 bases:

>100_bases
GGCAAACACAAGATATGTATCAACAATTTTTAAAAATGTCTAGACAAATTTTAAAATTATGCTATACTTTATGTATAGAA
TAATAGAAAGGAGGTTGAGC

Downstream 100 bases:

>100_bases
AGAGAGGAGATATTCATATGAAAAACTCAAAATTTATAGACCAATTTGCCACCTTTGCTGGTAAACTAGGGAACCAAATT
CATTTAAAAACCCTAAGAGA

Product: glycosyl hydrolase, family protein 1

Products: NA

Alternate protein names: Aryl-phospho-beta-D-glucosidase BglD; Glucomannan utilization protein D [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPEDFFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSI
QWSRLIKNLETGEPDPKGIAFYNAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDGKIGIILNLTPAYPRSNSPEDLE
ASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVLWSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMP
DQYFKEYEWPERRMNPYRGWEIFPKAIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIEFYEEHLRW
LHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSSGRWYRKVSDNNGFEVEIEE

Sequences:

>Translated_469_residues
MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPEDFFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSI
QWSRLIKNLETGEPDPKGIAFYNAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDGKIGIILNLTPAYPRSNSPEDLE
ASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVLWSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMP
DQYFKEYEWPERRMNPYRGWEIFPKAIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIEFYEEHLRW
LHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSSGRWYRKVSDNNGFEVEIEE
>Mature_469_residues
MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPEDFFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSI
QWSRLIKNLETGEPDPKGIAFYNAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDGKIGIILNLTPAYPRSNSPEDLE
ASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVLWSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMP
DQYFKEYEWPERRMNPYRGWEIFPKAIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIEFYEEHLRW
LHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSSGRWYRKVSDNNGFEVEIEE

Specific function: Phospho-beta-D-glucosidase that seems to be involved in the degradation of glucomannan. Is also capable of hydrolyzing aryl-phospho-beta-D-glucosides, although very weakly, and plays only a minor role, if any, in the degradation of these substrates in viv

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI13273313, Length=490, Percent_Identity=29.5918367346939, Blast_Score=192, Evalue=7e-49,
Organism=Homo sapiens, GI110681710, Length=484, Percent_Identity=28.099173553719, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI32481206, Length=489, Percent_Identity=29.6523517382413, Blast_Score=182, Evalue=5e-46,
Organism=Homo sapiens, GI24497614, Length=483, Percent_Identity=27.1221532091097, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI28376633, Length=475, Percent_Identity=26.3157894736842, Blast_Score=139, Evalue=5e-33,
Organism=Escherichia coli, GI1789070, Length=494, Percent_Identity=30.3643724696356, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI2367174, Length=489, Percent_Identity=30.0613496932515, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI2367270, Length=490, Percent_Identity=28.3673469387755, Blast_Score=187, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17539390, Length=469, Percent_Identity=30.0639658848614, Blast_Score=182, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17552856, Length=478, Percent_Identity=28.4518828451883, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI21356577, Length=486, Percent_Identity=27.1604938271605, Blast_Score=193, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 55198; Mature: 55198

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPEDFFDNVGPLVASNFFHT
CCCCEECCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHH
YTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFYNAIIEEAKKNQMDLVMNL
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEH
HHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKVHYWTTFNEPMVIPEAGYLYA
HHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEECCCCEEEE
FHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDGKIGIILNLTPAYPRSNSPEDLE
EECCCCCCCCHHHEEEEHCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCH
ASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVLWSHTEKELQLMKSNTVDFLGVNYY
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEECCCHHHHHHHHCCCEEEEECCCC
HPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRMNPYRGWEIFPKAIYDIAMIVKEEYGN
CCCCEECCCCHHHCCCCCCCHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHCCC
IPWFISENGMGVENEARFIDENGVIDDVYRIEFYEEHLRWLHKAIEEGSHCFGYHAWTAF
CCEEECCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEE
DCWSWNNAYKNRYGFISVDLETQKRTIKSSGRWYRKVSDNNGFEVEIEE
EECCCCCHHCCCCEEEEEEEHHHHHHHHCCCCEEEEECCCCCEEEEECC
>Mature Secondary Structure
MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPEDFFDNVGPLVASNFFHT
CCCCEECCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHH
YTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFYNAIIEEAKKNQMDLVMNL
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEH
HHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKVHYWTTFNEPMVIPEAGYLYA
HHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEECCCCEEEE
FHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDGKIGIILNLTPAYPRSNSPEDLE
EECCCCCCCCHHHEEEEHCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCH
ASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVLWSHTEKELQLMKSNTVDFLGVNYY
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEECCCHHHHHHHHCCCEEEEECCCC
HPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRMNPYRGWEIFPKAIYDIAMIVKEEYGN
CCCCEECCCCHHHCCCCCCCHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHCCC
IPWFISENGMGVENEARFIDENGVIDDVYRIEFYEEHLRWLHKAIEEGSHCFGYHAWTAF
CCEEECCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEE
DCWSWNNAYKNRYGFISVDLETQKRTIKSSGRWYRKVSDNNGFEVEIEE
EECCCCCHHCCCCEEEEEEEHHHHHHHHCCCCEEEEECCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]