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The map label for this gene is tuf [H]

Identifier: 116515356

GI number: 116515356

Start: 1334495

End: 1335691

Strand: Reverse

Name: tuf [H]

Synonym: SPD_1318

Alternate gene names: 116515356

Gene position: 1335691-1334495 (Counterclockwise)

Preceding gene: 116517081

Following gene: 116516784

Centisome position: 65.28

GC content: 44.28

Gene sequence:

>1197_bases
ATGGCAAAAGAAAAATACGATCGTAGTAAACCACACGTTAACATTGGTACTATCGGACACGTTGACCACGGTAAAACTAC
CCTAACTGCAGCTATCACAACTGTTTTGGCACGTCGCTTGCCTTCATCAGTTAACCAACCTAAAGACTATGCGTCTATCG
ATGCTGCTCCAGAAGAACGCGAACGCGGTATCACTATCAACACTGCGCACGTTGAGTACGAAACTGAAAAACGTCACTAC
GCTCACATCGACGCTCCAGGACACGCGGACTACGTTAAAAACATGATCACTGGTGCTGCTCAAATGGACGGAGCTATCCT
TGTAGTAGCTTCAACTGACGGACCAATGCCACAAACTCGTGAGCACATCCTTCTTTCACGTCAGGTTGGTGTTAAACACC
TTATCGTCTTCATGAACAAAGTTGACTTGGTTGACGACGAAGAATTGCTTGAATTGGTTGAAATGGAAATCCGTGACCTA
TTGTCAGAATACGACTTCCCAGGTGACGATCTTCCAGTTATCCAAGGTTCAGCACTTAAAGCTCTTGAAGGTGACTCTAA
ATACGAAGACATCGTTATGGAATTGATGAACACAGTTGATGAGTATATCCCAGAACCAGAACGTGACACTGACAAACCAT
TGCTTCTTCCAGTCGAGGACGTATTCTCAATCACTGGACGTGGTACAGTTGCTTCAGGACGTATCGACCGTGGTATCGTT
AAAGTCAACGACGAAATCGAAATCGTTGGTATCAAAGAAGAAACTCAAAAAGCAGTTGTTACTGGTGTTGAAATGTTCCG
TAAACAACTTGACGAAGGTCTTGCTGGAGATAACGTAGGTGTCCTTCTTCGTGGTGTTCAACGTGATGAAATCGAACGTG
GACAAGTTATCGCTAAACCAGGTTCAATCAACCCACACACTAAATTCAAAGGTGAAGTCTACATCCTTACTAAAGAAGAA
GGTGGACGTCACACTCCATTCTTCAACAACTACCGTCCACAATTCTACTTCCGTACTACTGACGTTACAGGTTCAATCGA
ACTTCCAGCAGGTACTGAAATGGTAATGCCTGGTGATAACGTGACAATCGACGTTGAGTTGATTCACCCAATCGCCGTAG
AACAAGGTACTACATTCTCTATCCGTGAGGGTGGACGTACTGTTGGTTCAGGTATGGTTACAGAAATCGAAGCTTAA

Upstream 100 bases:

>100_bases
CTTGCAATTTAGCTGAAATTTGATAAAATAGTAAGGAAAGTTAGACTGTATTGCCTACTGTCTATCTATAAAATATATTT
TATTGGAGGCTTTTACTCAA

Downstream 100 bases:

>100_bases
TTCGATTTAGTTCCCAGAAGAACAATTATTTAAGTTAGACACTAAAAGAATCTTGCTTGGCAAGGTTCTTTTTTTAGATA
TTGAACTAATACTCAATGAA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSVNQPKDYASIDAAPEERERGITINTAHVEYETEKRHY
AHIDAPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPVEDVFSITGRGTVASGRIDRGIV
KVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAGDNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEE
GGRHTPFFNNYRPQFYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGMVTEIEA

Sequences:

>Translated_398_residues
MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSVNQPKDYASIDAAPEERERGITINTAHVEYETEKRHY
AHIDAPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPVEDVFSITGRGTVASGRIDRGIV
KVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAGDNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEE
GGRHTPFFNNYRPQFYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGMVTEIEA
>Mature_397_residues
AKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSVNQPKDYASIDAAPEERERGITINTAHVEYETEKRHYA
HIDAPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDLL
SEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPVEDVFSITGRGTVASGRIDRGIVK
VNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAGDNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEG
GRHTPFFNNYRPQFYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGMVTEIEA

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=399, Percent_Identity=55.6390977443609, Blast_Score=458, Evalue=1e-129,
Organism=Homo sapiens, GI4503475, Length=448, Percent_Identity=29.0178571428571, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI4503471, Length=448, Percent_Identity=29.2410714285714, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI223555963, Length=443, Percent_Identity=29.3453724604966, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI5729864, Length=443, Percent_Identity=29.3453724604966, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI194018522, Length=321, Percent_Identity=29.9065420560748, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI194018520, Length=321, Percent_Identity=29.9065420560748, Blast_Score=128, Evalue=8e-30,
Organism=Homo sapiens, GI194097354, Length=321, Percent_Identity=29.9065420560748, Blast_Score=128, Evalue=8e-30,
Organism=Homo sapiens, GI46094014, Length=347, Percent_Identity=28.8184438040346, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI54607086, Length=315, Percent_Identity=25.7142857142857, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI4503507, Length=271, Percent_Identity=29.1512915129151, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI157426893, Length=294, Percent_Identity=27.891156462585, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1790412, Length=396, Percent_Identity=72.979797979798, Blast_Score=582, Evalue=1e-167,
Organism=Escherichia coli, GI1789737, Length=396, Percent_Identity=72.979797979798, Blast_Score=582, Evalue=1e-167,
Organism=Escherichia coli, GI2367247, Length=336, Percent_Identity=29.1666666666667, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1789108, Length=254, Percent_Identity=33.8582677165354, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI48994988, Length=277, Percent_Identity=30.3249097472924, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788922, Length=336, Percent_Identity=25.5952380952381, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=402, Percent_Identity=50.4975124378109, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25141371, Length=392, Percent_Identity=42.3469387755102, Blast_Score=320, Evalue=8e-88,
Organism=Caenorhabditis elegans, GI17552884, Length=448, Percent_Identity=30.5803571428571, Blast_Score=171, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17569207, Length=448, Percent_Identity=30.5803571428571, Blast_Score=171, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI32566303, Length=429, Percent_Identity=28.6713286713287, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI32566629, Length=340, Percent_Identity=30.2941176470588, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI115532067, Length=435, Percent_Identity=27.3563218390805, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=435, Percent_Identity=27.3563218390805, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17506081, Length=302, Percent_Identity=28.1456953642384, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17509919, Length=310, Percent_Identity=29.3548387096774, Blast_Score=98, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI32566301, Length=154, Percent_Identity=41.5584415584416, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17557151, Length=234, Percent_Identity=27.3504273504274, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=397, Percent_Identity=63.727959697733, Blast_Score=528, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6325337, Length=444, Percent_Identity=29.0540540540541, Blast_Score=166, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6319594, Length=444, Percent_Identity=29.0540540540541, Blast_Score=166, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6322937, Length=347, Percent_Identity=25.9365994236311, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6320863, Length=272, Percent_Identity=30.1470588235294, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6320377, Length=354, Percent_Identity=29.0960451977401, Blast_Score=93, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6323320, Length=249, Percent_Identity=29.3172690763052, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=143, Percent_Identity=32.1678321678322, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281363316, Length=394, Percent_Identity=55.5837563451777, Blast_Score=454, Evalue=1e-128,
Organism=Drosophila melanogaster, GI17864358, Length=394, Percent_Identity=55.5837563451777, Blast_Score=454, Evalue=1e-128,
Organism=Drosophila melanogaster, GI19921738, Length=390, Percent_Identity=47.1794871794872, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI45553807, Length=447, Percent_Identity=29.3064876957494, Blast_Score=169, Evalue=5e-42,
Organism=Drosophila melanogaster, GI45553816, Length=447, Percent_Identity=29.3064876957494, Blast_Score=169, Evalue=5e-42,
Organism=Drosophila melanogaster, GI24651721, Length=447, Percent_Identity=29.3064876957494, Blast_Score=169, Evalue=5e-42,
Organism=Drosophila melanogaster, GI17864154, Length=447, Percent_Identity=29.3064876957494, Blast_Score=169, Evalue=5e-42,
Organism=Drosophila melanogaster, GI24652838, Length=448, Percent_Identity=29.2410714285714, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI17137572, Length=448, Percent_Identity=29.2410714285714, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI45550900, Length=443, Percent_Identity=29.1196388261851, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI19922690, Length=307, Percent_Identity=30.6188925081433, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI17137380, Length=343, Percent_Identity=28.5714285714286, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24647046, Length=269, Percent_Identity=28.6245353159851, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24647048, Length=269, Percent_Identity=28.6245353159851, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24582462, Length=133, Percent_Identity=33.8345864661654, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43971; Mature: 43840

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSVNQPKDYASIDAAPEER
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCHHH
ERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTR
HCCCEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCH
EHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDLLSEYDFPGDDLPVIQGSALK
HHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHH
ALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPVEDVFSITGRGTVASGRIDRGIV
HCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHCCCCCEEECCCCCCCEE
KVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAGDNVGVLLRGVQRDEIERGQVIAKP
EECCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHCCCEEEECC
GSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQFYFRTTDVTGSIELPAGTEMVMPGDN
CCCCCCCEECCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCCEEEECCCC
VTIDVELIHPIAVEQGTTFSIREGGRTVGSGMVTEIEA
EEEEEEEECEEEECCCCEEEECCCCCCCCCCCEEEECC
>Mature Secondary Structure 
AKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSVNQPKDYASIDAAPEER
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCHHH
ERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTR
HCCCEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCH
EHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDLLSEYDFPGDDLPVIQGSALK
HHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHH
ALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPVEDVFSITGRGTVASGRIDRGIV
HCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHCCCCCEEECCCCCCCEE
KVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAGDNVGVLLRGVQRDEIERGQVIAKP
EECCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHCCCEEEECC
GSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQFYFRTTDVTGSIELPAGTEMVMPGDN
CCCCCCCEECCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCCEEEECCCC
VTIDVELIHPIAVEQGTTFSIREGGRTVGSGMVTEIEA
EEEEEEEECEEEECCCCEEEECCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA