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The map label for this gene is phnD [H]

Identifier: 116495889

GI number: 116495889

Start: 2431161

End: 2432108

Strand: Direct

Name: phnD [H]

Synonym: LSEI_2445

Alternate gene names: 116495889

Gene position: 2431161-2432108 (Clockwise)

Preceding gene: 116495888

Following gene: 116495890

Centisome position: 83.97

GC content: 43.25

Gene sequence:

>948_bases
ATGAAGTTCTGGAAAAGAATTGCAGTGGCTGGGATGGCAACCATTGCTGCGTTATCGCTAGCTGCTTGTGGTAGTAAGAG
TAGTTCTAGCAGCACAAGTACAAGCAATAGTTACACCCCCAAGTCATTAAATATTCAGTTTGTACCATCAAGCAACGCCA
ATACAATCGAAGCAAAAGCAAAACCTCTGGCTAGTTTGCTGAAGAAAGAGCTTGGCATCCCTGTCAAAGTCACCGTTTCG
ACAGACTACAACTCAATTGTCGAAGCTATGAAGAGTAAACAAGTCGATGTTGGGTTCTTACCACCTGATGGTTACGTTCA
GGCACACGCGCAAAAGGCAGCCGATGTTTTGTTGATGGCGCAACGATTTGGGATCAAACAGCCAGGTGGGAAAAGCACCG
ACAAACTGGTTGATTCATATCGTTCGATGATTGTTGTTAAAAAAGGCAGTAGCATCAAATCTTGGAAAGATCTCAAGAAT
AAAAAAATTGCCGTTCAAGATGTTACCAGTTCCGCTGGCTATATTTGGCCGATTGCCGAACTGTCCCAAAAGGGTCTCGA
CATTGTTAAACAAGATACCTTGGTTACTGTCAAAGGTCACGATCAAGGTGTCATGTCAGTGCTAAACGGTGATACCGATG
CCGCCTTTGTTTTTGAGGATGCCCGCAATATTGTCAAAAAAGATGTCCCTGACATTATGGACAAGGTTGAACCCATCTAC
TTCACAAAGCCAATTCCTAATGACACGATTTCCGTACGTCAGGACATGAGTGCCGCCTTCCGCAAAAAGCTTAGCAAGGC
TTTCATCGACATTGCTAAGACAAAAGAAGGTCATGCGATTATCTCAAGTATTTATACACATGAGGGCTATGTCAAAACCA
CAGATAGTGCCTTCAACATTGTGCGTAAGTATGACAAAATTGCCACTGGTGAATCAAAAAGCAAGTAA

Upstream 100 bases:

>100_bases
GTTCAAGTGACTTGTTCGTCTCAGTTCACTCTTTTGCGTCGCCGAGACAAGCAAGGTTCTGCTTACAAATGATTGATTCT
ACTTTTATGGAGGAAAACTG

Downstream 100 bases:

>100_bases
AGCAACGTTGCAAAAGCGCTAGCCACCTAGCGCTTTTGCTATACATGGAGGAAGTTTGCATGTCAGAAAGTAACCACCCA
GTTATTGAGTTTGACCACGT

Product: ABC-type phosphate/phosphonate transport system, periplasmic component

Products: 3-phospho-serine [Cytoplasm]; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MKFWKRIAVAGMATIAALSLAACGSKSSSSSTSTSNSYTPKSLNIQFVPSSNANTIEAKAKPLASLLKKELGIPVKVTVS
TDYNSIVEAMKSKQVDVGFLPPDGYVQAHAQKAADVLLMAQRFGIKQPGGKSTDKLVDSYRSMIVVKKGSSIKSWKDLKN
KKIAVQDVTSSAGYIWPIAELSQKGLDIVKQDTLVTVKGHDQGVMSVLNGDTDAAFVFEDARNIVKKDVPDIMDKVEPIY
FTKPIPNDTISVRQDMSAAFRKKLSKAFIDIAKTKEGHAIISSIYTHEGYVKTTDSAFNIVRKYDKIATGESKSK

Sequences:

>Translated_315_residues
MKFWKRIAVAGMATIAALSLAACGSKSSSSSTSTSNSYTPKSLNIQFVPSSNANTIEAKAKPLASLLKKELGIPVKVTVS
TDYNSIVEAMKSKQVDVGFLPPDGYVQAHAQKAADVLLMAQRFGIKQPGGKSTDKLVDSYRSMIVVKKGSSIKSWKDLKN
KKIAVQDVTSSAGYIWPIAELSQKGLDIVKQDTLVTVKGHDQGVMSVLNGDTDAAFVFEDARNIVKKDVPDIMDKVEPIY
FTKPIPNDTISVRQDMSAAFRKKLSKAFIDIAKTKEGHAIISSIYTHEGYVKTTDSAFNIVRKYDKIATGESKSK
>Mature_315_residues
MKFWKRIAVAGMATIAALSLAACGSKSSSSSTSTSNSYTPKSLNIQFVPSSNANTIEAKAKPLASLLKKELGIPVKVTVS
TDYNSIVEAMKSKQVDVGFLPPDGYVQAHAQKAADVLLMAQRFGIKQPGGKSTDKLVDSYRSMIVVKKGSSIKSWKDLKN
KKIAVQDVTSSAGYIWPIAELSQKGLDIVKQDTLVTVKGHDQGVMSVLNGDTDAAFVFEDARNIVKKDVPDIMDKVEPIY
FTKPIPNDTISVRQDMSAAFRKKLSKAFIDIAKTKEGHAIISSIYTHEGYVKTTDSAFNIVRKYDKIATGESKSK

Specific function: Part of the ABC transporter complex phnCDE involved in phosphate import. Responsible for phosphate binding [H]

COG id: COG3221

COG function: function code P; ABC-type phosphate/phosphonate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790543, Length=279, Percent_Identity=24.0143369175627, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015168
- InterPro:   IPR005770 [H]

Pfam domain/function: PF09084 NMT1 [H]

EC number: NA

Molecular weight: Translated: 34221; Mature: 34221

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFWKRIAVAGMATIAALSLAACGSKSSSSSTSTSNSYTPKSLNIQFVPSSNANTIEAKA
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEHHHH
KPLASLLKKELGIPVKVTVSTDYNSIVEAMKSKQVDVGFLPPDGYVQAHAQKAADVLLMA
HHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHH
QRFGIKQPGGKSTDKLVDSYRSMIVVKKGSSIKSWKDLKNKKIAVQDVTSSAGYIWPIAE
HHCCCCCCCCCCHHHHHHHHHCEEEEECCCCCHHHHHHCCCEEEEEECCCCCCEEEEHHH
LSQKGLDIVKQDTLVTVKGHDQGVMSVLNGDTDAAFVFEDARNIVKKDVPDIMDKVEPIY
HHHCCCCEEECCCEEEEECCCCHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCEE
FTKPIPNDTISVRQDMSAAFRKKLSKAFIDIAKTKEGHAIISSIYTHEGYVKTTDSAFNI
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECHHHHHH
VRKYDKIATGESKSK
HHHHHHHHCCCCCCC
>Mature Secondary Structure
MKFWKRIAVAGMATIAALSLAACGSKSSSSSTSTSNSYTPKSLNIQFVPSSNANTIEAKA
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEHHHH
KPLASLLKKELGIPVKVTVSTDYNSIVEAMKSKQVDVGFLPPDGYVQAHAQKAADVLLMA
HHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHH
QRFGIKQPGGKSTDKLVDSYRSMIVVKKGSSIKSWKDLKNKKIAVQDVTSSAGYIWPIAE
HHCCCCCCCCCCHHHHHHHHHCEEEEECCCCCHHHHHHCCCEEEEEECCCCCCEEEEHHH
LSQKGLDIVKQDTLVTVKGHDQGVMSVLNGDTDAAFVFEDARNIVKKDVPDIMDKVEPIY
HHHCCCCEEECCCEEEEECCCCHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCEE
FTKPIPNDTISVRQDMSAAFRKKLSKAFIDIAKTKEGHAIISSIYTHEGYVKTTDSAFNI
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECHHHHHH
VRKYDKIATGESKSK
HHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA