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The map label for this gene is insI1 [C]

Identifier: 116495472

GI number: 116495472

Start: 1986192

End: 1987121

Strand: Direct

Name: insI1 [C]

Synonym: LSEI_2008

Alternate gene names: 116495472

Gene position: 1986192-1987121 (Clockwise)

Preceding gene: 116495471

Following gene: 116495474

Centisome position: 68.6

GC content: 40.97

Gene sequence:

>930_bases
TTGTCTAGTATAACCTATTCCGAACGAATTAAAATCGAAACCTTTTGTGAACTAGGGCTGTCCAATATCCAAATGGGCGT
TCGGCTGAACCGATCACCGTCAACAATTTCTTATGAATTATCTCGATGTCAACCTTATCAGGCTGAATTAGCACAAACAG
ATGCCGAATACAAGCGATCACGATGTGGTCGGAAAACTAAGCTGAGCGATGAGTTAAAGCAAAAAATTCTCAACCATTTA
CGTCTAAGCTGGTCACCAGGAATGATTGCTCACGAATTTAAACTAGCTACTAAATCTATTTATAATTGGCTAAATCAGGG
GAGAATTGGTTTCTCCTTGAATGATCTACCTGAACATGGCGTACGCCAACGGCGTAACGTTGACCAACGATCCAAATATA
ATCAATCTTTGGGGCGATCAATTGAACAGCGTCCCATGATGATTAATCAACGTAATCGCATCGGCGATTTTGAACTAGAT
ACAGTCGTTGGTCCTCGTGGGCATAGTAAGGCAGTTTTATTAACTTTAATCGATCGAAAATCACGGTTCCTTTGGGCATA
CCGGTTAAAAGATCGGACGACAGCGACTGTTAATGAAGCACTAACTAAGTTCCTAACCACTTTTAATGGTCCGGTGCACA
GCTTTACTGTGGACCGTGGCACTGAGTTTAGTGGGCTAGTATCACTTGAATCACAATATGGTATTAAGACCTATTACTGC
CATGCTTATACTCCAGCTGAACGTGGTAGTAATGAACGCTTTAATCGGAATTTACGTTATTTTTATCCTAAAGGGACTCG
TTTTGAGCACATTAGTGCTCAAGATTTAACGACGACGTTACTCCAAATTAACCAGCGACCGCTTAAAATACTCGACTGGC
AAACACCGTATCAGGTTATGCTGACAAATTTGTCCAAAAATTCGGATTAA

Upstream 100 bases:

>100_bases
AGGTAGATTGTAAAATTAATCCGAACGCTGTTCGGACAAAAAAGATCAGCTTCCTTTAAAATGGTGTTTACCACAAACCC
ATCTTTTAGGAGCTGATCTT

Downstream 100 bases:

>100_bases
ATTTGCAATCTACCAAAAGGATATTTGTAGAACAATTAAGCCGGAAATTATCAATCCAAAGGAGGTAAAATCAGGAACTT
CAAGTGAAAATTTCATCGTG

Product: IS30 family transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MSSITYSERIKIETFCELGLSNIQMGVRLNRSPSTISYELSRCQPYQAELAQTDAEYKRSRCGRKTKLSDELKQKILNHL
RLSWSPGMIAHEFKLATKSIYNWLNQGRIGFSLNDLPEHGVRQRRNVDQRSKYNQSLGRSIEQRPMMINQRNRIGDFELD
TVVGPRGHSKAVLLTLIDRKSRFLWAYRLKDRTTATVNEALTKFLTTFNGPVHSFTVDRGTEFSGLVSLESQYGIKTYYC
HAYTPAERGSNERFNRNLRYFYPKGTRFEHISAQDLTTTLLQINQRPLKILDWQTPYQVMLTNLSKNSD

Sequences:

>Translated_309_residues
MSSITYSERIKIETFCELGLSNIQMGVRLNRSPSTISYELSRCQPYQAELAQTDAEYKRSRCGRKTKLSDELKQKILNHL
RLSWSPGMIAHEFKLATKSIYNWLNQGRIGFSLNDLPEHGVRQRRNVDQRSKYNQSLGRSIEQRPMMINQRNRIGDFELD
TVVGPRGHSKAVLLTLIDRKSRFLWAYRLKDRTTATVNEALTKFLTTFNGPVHSFTVDRGTEFSGLVSLESQYGIKTYYC
HAYTPAERGSNERFNRNLRYFYPKGTRFEHISAQDLTTTLLQINQRPLKILDWQTPYQVMLTNLSKNSD
>Mature_308_residues
SSITYSERIKIETFCELGLSNIQMGVRLNRSPSTISYELSRCQPYQAELAQTDAEYKRSRCGRKTKLSDELKQKILNHLR
LSWSPGMIAHEFKLATKSIYNWLNQGRIGFSLNDLPEHGVRQRRNVDQRSKYNQSLGRSIEQRPMMINQRNRIGDFELDT
VVGPRGHSKAVLLTLIDRKSRFLWAYRLKDRTTATVNEALTKFLTTFNGPVHSFTVDRGTEFSGLVSLESQYGIKTYYCH
AYTPAERGSNERFNRNLRYFYPKGTRFEHISAQDLTTTLLQINQRPLKILDWQTPYQVMLTNLSKNSD

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG2826

COG function: function code L; Transposase and inactivated derivatives, IS30 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1786450, Length=325, Percent_Identity=29.8461538461538, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1790736, Length=325, Percent_Identity=29.8461538461538, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1787671, Length=325, Percent_Identity=29.8461538461538, Blast_Score=122, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR001598 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 35907; Mature: 35776

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSITYSERIKIETFCELGLSNIQMGVRLNRSPSTISYELSRCQPYQAELAQTDAEYKRS
CCCCCCHHCEEHHHHHHCCCCCEEEEEEECCCCCEEEEEHHHCCCHHHHHHHHHHHHHHH
RCGRKTKLSDELKQKILNHLRLSWSPGMIAHEFKLATKSIYNWLNQGRIGFSLNDLPEHG
HCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
VRQRRNVDQRSKYNQSLGRSIEQRPMMINQRNRIGDFELDTVVGPRGHSKAVLLTLIDRK
HHHHCCCHHHHHHHHHHCCCHHHCCCEECCCCCCCCEEEEEEECCCCCCCEEEEEEECCC
SRFLWAYRLKDRTTATVNEALTKFLTTFNGPVHSFTVDRGTEFSGLVSLESQYGIKTYYC
CCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEECCCCCCEEEEE
HAYTPAERGSNERFNRNLRYFYPKGTRFEHISAQDLTTTLLQINQRPLKILDWQTPYQVM
EEECHHHCCCCHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHH
LTNLSKNSD
HHHHCCCCC
>Mature Secondary Structure 
SSITYSERIKIETFCELGLSNIQMGVRLNRSPSTISYELSRCQPYQAELAQTDAEYKRS
CCCCCHHCEEHHHHHHCCCCCEEEEEEECCCCCEEEEEHHHCCCHHHHHHHHHHHHHHH
RCGRKTKLSDELKQKILNHLRLSWSPGMIAHEFKLATKSIYNWLNQGRIGFSLNDLPEHG
HCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
VRQRRNVDQRSKYNQSLGRSIEQRPMMINQRNRIGDFELDTVVGPRGHSKAVLLTLIDRK
HHHHCCCHHHHHHHHHHCCCHHHCCCEECCCCCCCCEEEEEEECCCCCCCEEEEEEECCC
SRFLWAYRLKDRTTATVNEALTKFLTTFNGPVHSFTVDRGTEFSGLVSLESQYGIKTYYC
CCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEECCCCCCEEEEE
HAYTPAERGSNERFNRNLRYFYPKGTRFEHISAQDLTTTLLQINQRPLKILDWQTPYQVM
EEECHHHCCCCHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHH
LTNLSKNSD
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8393068 [H]