Click here to switch to the map view.

The map label for this gene is insF1 [H]

Identifier: 116495373

GI number: 116495373

Start: 1902718

End: 1903473

Strand: Direct

Name: insF1 [H]

Synonym: LSEI_1907

Alternate gene names: 116495373

Gene position: 1902718-1903473 (Clockwise)

Preceding gene: 116495372

Following gene: 116495392

Centisome position: 65.72

GC content: 44.71

Gene sequence:

>756_bases
TTGAAACAACGGATTCTGCGGATCTTTGCGGAATTTAAGCAGCGATACGGTGTTATGAAGATCCACCATGAATTGAATCT
GGAACTTCAACCACTGCAGCTTCGGTGCAGTCCACGACGGATTTCCCGGCTCATGAAGGAACTGGATATCCACTCCGTTA
CCGTCAATAAGTGGAAAGCGGCTTCGGCTTCCAAAACCAAGGTTGAACAGCGTCCCAACTTGCTTAAGCAGGATTTCTCG
ACCACTGGTTTAAATCAAAAATGGACCGCTGATATGACCTATATTCAAACGAAGCGTAATGGCTGGTGTTACTTATCAAC
CATCATGGACCTGCACTCACGACGGATTATCGGCTATTCGTTCTCAAAAAAGATGGATACTGATTTAGTCTTAAAGACCC
TTGAAAGCGCGGTTAAAAATCGAACCATTACTGGGGACCTGATTATCCATACGGATTTAGGATCACAGTATACCAGCGAT
GATTACAATCAACGTTTAACTGAGCTACATATCCGCCACTCATACAGCCGTAAGGGTTGTCCGTATGATAATGCGCCAAT
GGAATCCTTTCACGCTTCCCTCAAAAAGGAATGTGTTTATCCAGTGCCGGTCTTTGAAGATTATGAAACTGCCGCTGCCG
TCCTTTTTGAATATGTGCATGCTTTCTACAATAGGAAGAGAATTCATAGTTCACTGGGCTACCAGACCCCCTTACAAGTT
GAAATTGCAACACTTACGAGCCAAATGGCCGCCTGA

Upstream 100 bases:

>100_bases
ACCAGGTAACCAAGATGTGCCGAATCCTCGGTGTTTCCAGAGCTCAGTATTATCGTTATCGATCCCCCAAACCTTCAAAA
CGCCGGCCGAAGATGCGAGC

Downstream 100 bases:

>100_bases
TTTAATGCTTTCCAGGGTTCAAATAAGTTATTAATCGCGATTAATGATTTATTTGAGCTGTGGAAGGTAAACGCGGTTCT
GAATGTCTCTTAAAATCTGT

Product: transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MKQRILRIFAEFKQRYGVMKIHHELNLELQPLQLRCSPRRISRLMKELDIHSVTVNKWKAASASKTKVEQRPNLLKQDFS
TTGLNQKWTADMTYIQTKRNGWCYLSTIMDLHSRRIIGYSFSKKMDTDLVLKTLESAVKNRTITGDLIIHTDLGSQYTSD
DYNQRLTELHIRHSYSRKGCPYDNAPMESFHASLKKECVYPVPVFEDYETAAAVLFEYVHAFYNRKRIHSSLGYQTPLQV
EIATLTSQMAA

Sequences:

>Translated_251_residues
MKQRILRIFAEFKQRYGVMKIHHELNLELQPLQLRCSPRRISRLMKELDIHSVTVNKWKAASASKTKVEQRPNLLKQDFS
TTGLNQKWTADMTYIQTKRNGWCYLSTIMDLHSRRIIGYSFSKKMDTDLVLKTLESAVKNRTITGDLIIHTDLGSQYTSD
DYNQRLTELHIRHSYSRKGCPYDNAPMESFHASLKKECVYPVPVFEDYETAAAVLFEYVHAFYNRKRIHSSLGYQTPLQV
EIATLTSQMAA
>Mature_251_residues
MKQRILRIFAEFKQRYGVMKIHHELNLELQPLQLRCSPRRISRLMKELDIHSVTVNKWKAASASKTKVEQRPNLLKQDFS
TTGLNQKWTADMTYIQTKRNGWCYLSTIMDLHSRRIIGYSFSKKMDTDLVLKTLESAVKNRTITGDLIIHTDLGSQYTSD
DYNQRLTELHIRHSYSRKGCPYDNAPMESFHASLKKECVYPVPVFEDYETAAAVLFEYVHAFYNRKRIHSSLGYQTPLQV
EIATLTSQMAA

Specific function: Involved in the transposition of the insertion sequence IS3 [H]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=197, Percent_Identity=36.0406091370558, Blast_Score=124, Evalue=8e-29,
Organism=Escherichia coli, GI1788405, Length=242, Percent_Identity=36.7768595041322, Blast_Score=157, Evalue=7e-40,
Organism=Escherichia coli, GI1787263, Length=242, Percent_Identity=36.7768595041322, Blast_Score=157, Evalue=7e-40,
Organism=Escherichia coli, GI1786753, Length=242, Percent_Identity=36.7768595041322, Blast_Score=157, Evalue=7e-40,
Organism=Escherichia coli, GI1786570, Length=242, Percent_Identity=36.7768595041322, Blast_Score=157, Evalue=7e-40,
Organism=Escherichia coli, GI1786490, Length=242, Percent_Identity=36.7768595041322, Blast_Score=157, Evalue=7e-40,
Organism=Escherichia coli, GI1789981, Length=241, Percent_Identity=32.3651452282158, Blast_Score=106, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 29230; Mature: 29230

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQRILRIFAEFKQRYGVMKIHHELNLELQPLQLRCSPRRISRLMKELDIHSVTVNKWKA
CHHHHHHHHHHHHHHCCEEEEEHHCCCEEECCEEECCHHHHHHHHHHHCCCEEEECCCCC
ASASKTKVEQRPNLLKQDFSTTGLNQKWTADMTYIQTKRNGWCYLSTIMDLHSRRIIGYS
CCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCEEEEECCCCCEEHHHHHHHHHCEEEEEE
FSKKMDTDLVLKTLESAVKNRTITGDLIIHTDLGSQYTSDDYNQRLTELHIRHSYSRKGC
CCCCCCHHHHHHHHHHHHHCCCEECEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCC
PYDNAPMESFHASLKKECVYPVPVFEDYETAAAVLFEYVHAFYNRKRIHSSLGYQTPLQV
CCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
EIATLTSQMAA
EHHHHHHHHCC
>Mature Secondary Structure
MKQRILRIFAEFKQRYGVMKIHHELNLELQPLQLRCSPRRISRLMKELDIHSVTVNKWKA
CHHHHHHHHHHHHHHCCEEEEEHHCCCEEECCEEECCHHHHHHHHHHHCCCEEEECCCCC
ASASKTKVEQRPNLLKQDFSTTGLNQKWTADMTYIQTKRNGWCYLSTIMDLHSRRIIGYS
CCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCEEEEECCCCCEEHHHHHHHHHCEEEEEE
FSKKMDTDLVLKTLESAVKNRTITGDLIIHTDLGSQYTSDDYNQRLTELHIRHSYSRKGC
CCCCCCHHHHHHHHHHHHHCCCEECEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCC
PYDNAPMESFHASLKKECVYPVPVFEDYETAAAVLFEYVHAFYNRKRIHSSLGYQTPLQV
CCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
EIATLTSQMAA
EHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987844; 9278503 [H]