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The map label for this gene is nrdE [H]

Identifier: 116494954

GI number: 116494954

Start: 1460845

End: 1463016

Strand: Direct

Name: nrdE [H]

Synonym: LSEI_1468

Alternate gene names: 116494954

Gene position: 1460845-1463016 (Clockwise)

Preceding gene: 116494953

Following gene: 116494955

Centisome position: 50.46

GC content: 46.18

Gene sequence:

>2172_bases
ATGTCATTAAAAACAATTAGTCCGGATCAAGTCACCTATTACGCGCTCAACAACGAGATCAACATTCCTGTGAATGACCA
GATTCCGTTGAACAAAGACAAGGAAGCGTTGCAGGCATTTTTGACCGAAAATGTAGCGCCAAATACGATGCAATTTGATT
CGCTGGCTGATCGACTGAAGTATTTAGTCGATAACCACTATTATGAAGCCGACTTTTTGAACAAGTATCAGCCAGCTTTT
TTGGAAAAACTGGATCAATTTCTTTCAGCGCAGCATTTTCAGTTCAAATCCTTCATGGCCGCCTACAAATACTATGCACA
GTATGCCTTGAAAACCGATGACGGTACCCAGTATTTAGAAGATTTTAAAGATCGGGTTTTTGCCAACGCACTTTTCTTTG
CAGACGGTAACGAGGACTTGGCAGTTGACCTTGCTGATGAAATGATTCATCAACGTTATCAACCGGCCACCCCTTCCTTC
TTGAATGCTGGCCGGGCCCGCCGTGGCGAACTGGTTTCATGTTTTTTGTTGCAAATCACTGATGACATGAATTCAATTGG
CCGGGCGATCAATTCGGCCTTGGAATTATCCCGAATTGGTGGCGGCGTTGGTCTCACCTTGTCCAACCTACGTGAAGCCG
GTGCCCCAATTAAGGGCATTGAAGGTGCCGCTAGCGGCGTCTTGCCAGTCATGAAGCTGTTGGAAGACTCTTTTTCCTAT
AGCAACCAATTGGGCCAGCGACAAGGTGCCGGTGTTGTGTATCTGAACGTTTTCCATCCTGACATCATCAGTTTCTTAGG
TGCCAAGAAGGAAAACGCGGACGAAAAGTACCGGGTTAAAACACTCAGTCTTGGCGTTGTCGTTCCGGATAAGTATTATG
ACCTCATTAAGGCTAATGCCGATATGTACCTCTTCTCCCCTTACTCGGTTGAACGGGTTTACGGGAAGCCGTTCTCTTAT
GTTGATATCACCAAGGAATATGACAACATGGTTGCCAATCCGGCCATTAAGAAGTACAAGATTAAGGCCCGTGATCTAGA
AAACGAAATCTCCAAACTTCAGCAGGAATCTGGTTATCCTTATATTATCAACATTGATACGGCAAATCGCGCCAACCCAA
TCGAAGGCAAAATCATCATGAGTAATCTGTGCTCGGAAATCATGCAGGTTCAGGTGCCTTCCAAGTTGAACAATAAACAG
GAATATGAAGTGCTGGGTACTGATGTATCTTGCAACTTAGGTAGCACGAATATTCCCAACCTGATGCATAGCCGCGACTT
TGGCCGCAGTGTCGAAGCAATGGTTCGTGCTTTGACTTATGTCACGGACCACAGCAACATCGACGTTGTACCTTCCGTCC
AGCACGGTAATCACCAGGCTCACAGCATTGGCCTTGGTGCGATGGGCTTGCACACTTATTTTGCGAAGAATCATATGTTC
TACGGTTCGCCTGAAAGCTTGGACTTCACCAATATTTACTTCCTTTTGCTGAATTACTACACCCTGAAGGCATCCAATAA
AATTGCCCAGGAACGCGGCGAAAGCTTCCACAACTTCGAAAACAGCAAGTATGCGGACGGTAGCTACTTCGATAAGTACA
CCGAGCAAGTATGGGCACCGAAGTATGACAAGGTTCGCGCGCTGTTTGATGGCATTCACATTCCGACGCAGGCTGATTGG
GAAGCGCTGAAGACCAGTGTCATGAAAGATGGTCTCTACCATCAGAACCGCATGGCTGTTGCGCCAAATGGGTCGATCAG
TTACATCAACGACACCAGTGCATCATTACAGCCGATCGTTAACCGCATTGAAGATCGGCAAGAAAAGAAGATCGGCACGA
TTTACTACCCTGCTCCAGACCTTTCTAATGACACGATGCCTTACTATCAAAGTGCCTATGATATTGATATGCGTAAAGTG
ATTGACGTTTACGCTGCTGCCCAGCAGCATGTCGATCAAGGAATGGCCATGACGTTGTTCATGCGCAGTACCATTCCGGC
TGGCCTTTATCCTTGGAAAGATGGCCGAACCGATAAAATGACCACCCGTGATTTAAATATTTTGCGTAATTACGCACATC
ATAAGGGCATCAAGTCGATTTACTACATCCGGACTTACACGGATGATCAAGAAGAAATCGGCAGCAACGCTTGTGAAAGC
TGCTCAATCTGA

Upstream 100 bases:

>100_bases
ACCGGACGCCTTGAAGCAACTGGCTGTTTGATCAGCCAGTTTTTTTATCGCATCACAACGCAACCAGCTATTATAAAACG
CAGCGAAAGCAGGGTTTAGC

Downstream 100 bases:

>100_bases
TAATCTTTTAACAGGGGGAGTTTCCCCTGTTTTTACATAAGGGAGCGTAAACATGGCAAAACAGTATATTGCGATCAATT
GGAACGCGATCGAAGACGAA

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase R1 subunit [H]

Number of amino acids: Translated: 723; Mature: 722

Protein sequence:

>723_residues
MSLKTISPDQVTYYALNNEINIPVNDQIPLNKDKEALQAFLTENVAPNTMQFDSLADRLKYLVDNHYYEADFLNKYQPAF
LEKLDQFLSAQHFQFKSFMAAYKYYAQYALKTDDGTQYLEDFKDRVFANALFFADGNEDLAVDLADEMIHQRYQPATPSF
LNAGRARRGELVSCFLLQITDDMNSIGRAINSALELSRIGGGVGLTLSNLREAGAPIKGIEGAASGVLPVMKLLEDSFSY
SNQLGQRQGAGVVYLNVFHPDIISFLGAKKENADEKYRVKTLSLGVVVPDKYYDLIKANADMYLFSPYSVERVYGKPFSY
VDITKEYDNMVANPAIKKYKIKARDLENEISKLQQESGYPYIINIDTANRANPIEGKIIMSNLCSEIMQVQVPSKLNNKQ
EYEVLGTDVSCNLGSTNIPNLMHSRDFGRSVEAMVRALTYVTDHSNIDVVPSVQHGNHQAHSIGLGAMGLHTYFAKNHMF
YGSPESLDFTNIYFLLLNYYTLKASNKIAQERGESFHNFENSKYADGSYFDKYTEQVWAPKYDKVRALFDGIHIPTQADW
EALKTSVMKDGLYHQNRMAVAPNGSISYINDTSASLQPIVNRIEDRQEKKIGTIYYPAPDLSNDTMPYYQSAYDIDMRKV
IDVYAAAQQHVDQGMAMTLFMRSTIPAGLYPWKDGRTDKMTTRDLNILRNYAHHKGIKSIYYIRTYTDDQEEIGSNACES
CSI

Sequences:

>Translated_723_residues
MSLKTISPDQVTYYALNNEINIPVNDQIPLNKDKEALQAFLTENVAPNTMQFDSLADRLKYLVDNHYYEADFLNKYQPAF
LEKLDQFLSAQHFQFKSFMAAYKYYAQYALKTDDGTQYLEDFKDRVFANALFFADGNEDLAVDLADEMIHQRYQPATPSF
LNAGRARRGELVSCFLLQITDDMNSIGRAINSALELSRIGGGVGLTLSNLREAGAPIKGIEGAASGVLPVMKLLEDSFSY
SNQLGQRQGAGVVYLNVFHPDIISFLGAKKENADEKYRVKTLSLGVVVPDKYYDLIKANADMYLFSPYSVERVYGKPFSY
VDITKEYDNMVANPAIKKYKIKARDLENEISKLQQESGYPYIINIDTANRANPIEGKIIMSNLCSEIMQVQVPSKLNNKQ
EYEVLGTDVSCNLGSTNIPNLMHSRDFGRSVEAMVRALTYVTDHSNIDVVPSVQHGNHQAHSIGLGAMGLHTYFAKNHMF
YGSPESLDFTNIYFLLLNYYTLKASNKIAQERGESFHNFENSKYADGSYFDKYTEQVWAPKYDKVRALFDGIHIPTQADW
EALKTSVMKDGLYHQNRMAVAPNGSISYINDTSASLQPIVNRIEDRQEKKIGTIYYPAPDLSNDTMPYYQSAYDIDMRKV
IDVYAAAQQHVDQGMAMTLFMRSTIPAGLYPWKDGRTDKMTTRDLNILRNYAHHKGIKSIYYIRTYTDDQEEIGSNACES
CSI
>Mature_722_residues
SLKTISPDQVTYYALNNEINIPVNDQIPLNKDKEALQAFLTENVAPNTMQFDSLADRLKYLVDNHYYEADFLNKYQPAFL
EKLDQFLSAQHFQFKSFMAAYKYYAQYALKTDDGTQYLEDFKDRVFANALFFADGNEDLAVDLADEMIHQRYQPATPSFL
NAGRARRGELVSCFLLQITDDMNSIGRAINSALELSRIGGGVGLTLSNLREAGAPIKGIEGAASGVLPVMKLLEDSFSYS
NQLGQRQGAGVVYLNVFHPDIISFLGAKKENADEKYRVKTLSLGVVVPDKYYDLIKANADMYLFSPYSVERVYGKPFSYV
DITKEYDNMVANPAIKKYKIKARDLENEISKLQQESGYPYIINIDTANRANPIEGKIIMSNLCSEIMQVQVPSKLNNKQE
YEVLGTDVSCNLGSTNIPNLMHSRDFGRSVEAMVRALTYVTDHSNIDVVPSVQHGNHQAHSIGLGAMGLHTYFAKNHMFY
GSPESLDFTNIYFLLLNYYTLKASNKIAQERGESFHNFENSKYADGSYFDKYTEQVWAPKYDKVRALFDGIHIPTQADWE
ALKTSVMKDGLYHQNRMAVAPNGSISYINDTSASLQPIVNRIEDRQEKKIGTIYYPAPDLSNDTMPYYQSAYDIDMRKVI
DVYAAAQQHVDQGMAMTLFMRSTIPAGLYPWKDGRTDKMTTRDLNILRNYAHHKGIKSIYYIRTYTDDQEEIGSNACESC
SI

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=592, Percent_Identity=25.8445945945946, Blast_Score=163, Evalue=7e-40,
Organism=Escherichia coli, GI1789030, Length=723, Percent_Identity=51.313969571231, Blast_Score=751, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=592, Percent_Identity=24.4932432432432, Blast_Score=156, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17555242, Length=583, Percent_Identity=25.3859348198971, Blast_Score=167, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6322123, Length=614, Percent_Identity=23.7785016286645, Blast_Score=166, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6320914, Length=600, Percent_Identity=23.5, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI17352481, Length=486, Percent_Identity=25.3086419753086, Blast_Score=150, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 81866; Mature: 81735

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKTISPDQVTYYALNNEINIPVNDQIPLNKDKEALQAFLTENVAPNTMQFDSLADRLK
CCCCCCCCCCEEEEEECCEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCEEHHHHHHHHH
YLVDNHYYEADFLNKYQPAFLEKLDQFLSAQHFQFKSFMAAYKYYAQYALKTDDGTQYLE
HHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHH
DFKDRVFANALFFADGNEDLAVDLADEMIHQRYQPATPSFLNAGRARRGELVSCFLLQIT
HHHHHHHHHEEEEECCCCCEEEEHHHHHHHHHCCCCCCHHHHCCCCCCCCHHEEEEEHHH
DDMNSIGRAINSALELSRIGGGVGLTLSNLREAGAPIKGIEGAASGVLPVMKLLEDSFSY
HHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
SNQLGQRQGAGVVYLNVFHPDIISFLGAKKENADEKYRVKTLSLGVVVPDKYYDLIKANA
HHHHHHCCCCCEEEEEECCHHHHHHHCCCCCCCCCCEEEEEEEEEEEECHHHHHHHHCCC
DMYLFSPYSVERVYGKPFSYVDITKEYDNMVANPAIKKYKIKARDLENEISKLQQESGYP
CEEEECCCCHHHHCCCCCCEEECCHHHHHHHCCCCHHHEEEHHHHHHHHHHHHHHHCCCC
YIINIDTANRANPIEGKIIMSNLCSEIMQVQVPSKLNNKQEYEVLGTDVSCNLGSTNIPN
EEEEECCCCCCCCCCCHHHHHHHHHHHHHEECCHHCCCCCCEEEECCCEEECCCCCCCCH
LMHSRDFGRSVEAMVRALTYVTDHSNIDVVPSVQHGNHQAHSIGLGAMGLHTYFAKNHMF
HHHHHHCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHEECCCHHHHHHHHHHCCEE
YGSPESLDFTNIYFLLLNYYTLKASNKIAQERGESFHNFENSKYADGSYFDKYTEQVWAP
ECCCCCCCHHHHHHHHHHHHHEECCHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHCCC
KYDKVRALFDGIHIPTQADWEALKTSVMKDGLYHQNRMAVAPNGSISYINDTSASLQPIV
CHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCEEEECCCCHHHHHHH
NRIEDRQEKKIGTIYYPAPDLSNDTMPYYQSAYDIDMRKVIDVYAAAQQHVDQGMAMTLF
HHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCHHEEHH
MRSTIPAGLYPWKDGRTDKMTTRDLNILRNYAHHKGIKSIYYIRTYTDDQEEIGSNACES
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHCCCCHHC
CSI
CCC
>Mature Secondary Structure 
SLKTISPDQVTYYALNNEINIPVNDQIPLNKDKEALQAFLTENVAPNTMQFDSLADRLK
CCCCCCCCCEEEEEECCEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCEEHHHHHHHHH
YLVDNHYYEADFLNKYQPAFLEKLDQFLSAQHFQFKSFMAAYKYYAQYALKTDDGTQYLE
HHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHH
DFKDRVFANALFFADGNEDLAVDLADEMIHQRYQPATPSFLNAGRARRGELVSCFLLQIT
HHHHHHHHHEEEEECCCCCEEEEHHHHHHHHHCCCCCCHHHHCCCCCCCCHHEEEEEHHH
DDMNSIGRAINSALELSRIGGGVGLTLSNLREAGAPIKGIEGAASGVLPVMKLLEDSFSY
HHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
SNQLGQRQGAGVVYLNVFHPDIISFLGAKKENADEKYRVKTLSLGVVVPDKYYDLIKANA
HHHHHHCCCCCEEEEEECCHHHHHHHCCCCCCCCCCEEEEEEEEEEEECHHHHHHHHCCC
DMYLFSPYSVERVYGKPFSYVDITKEYDNMVANPAIKKYKIKARDLENEISKLQQESGYP
CEEEECCCCHHHHCCCCCCEEECCHHHHHHHCCCCHHHEEEHHHHHHHHHHHHHHHCCCC
YIINIDTANRANPIEGKIIMSNLCSEIMQVQVPSKLNNKQEYEVLGTDVSCNLGSTNIPN
EEEEECCCCCCCCCCCHHHHHHHHHHHHHEECCHHCCCCCCEEEECCCEEECCCCCCCCH
LMHSRDFGRSVEAMVRALTYVTDHSNIDVVPSVQHGNHQAHSIGLGAMGLHTYFAKNHMF
HHHHHHCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHEECCCHHHHHHHHHHCCEE
YGSPESLDFTNIYFLLLNYYTLKASNKIAQERGESFHNFENSKYADGSYFDKYTEQVWAP
ECCCCCCCHHHHHHHHHHHHHEECCHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHCCC
KYDKVRALFDGIHIPTQADWEALKTSVMKDGLYHQNRMAVAPNGSISYINDTSASLQPIV
CHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCEEEECCCCHHHHHHH
NRIEDRQEKKIGTIYYPAPDLSNDTMPYYQSAYDIDMRKVIDVYAAAQQHVDQGMAMTLF
HHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCHHEEHH
MRSTIPAGLYPWKDGRTDKMTTRDLNILRNYAHHKGIKSIYYIRTYTDDQEEIGSNACES
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHCCCCHHC
CSI
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]