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The map label for this gene is ywbI [H]

Identifier: 116494899

GI number: 116494899

Start: 1404402

End: 1405370

Strand: Direct

Name: ywbI [H]

Synonym: LSEI_1413

Alternate gene names: 116494899

Gene position: 1404402-1405370 (Clockwise)

Preceding gene: 116494898

Following gene: 116494900

Centisome position: 48.51

GC content: 43.76

Gene sequence:

>969_bases
ATGAAAACGAAACAAGAGCGCATTTTTTCCTCAAAGACACTAACTTATTTTCTGCAATTGGCAGAAACCATGAACTACAC
CCAAGCCGCCCAATTGCTTGGCATTACCCAGCCAGCCTTAACACAGCAGATCAAGAAATTGGAACGGACAGTGGGGGCAC
CGCTGTTTTATTCTGTCGGGAAGAAACTGCATTTATCTGACGCTGGTCAGACCATGTTAAAGTCAACCCATGAGATCTAC
GAGATCTTGAACCAGACAACCGACGAAATTCAACAAACAACCAGTGCAACCGTCGGAAAAATCAATATTGGGTTATTAAG
CTCAATCGAGGATAGCGCCTTTATTGCGTTCATTGCTCATTACTATCAACTGAATCCTGAAGTTGAAGTAACGCTACGGA
TGATGACCCGTCACGAAATTTGGGAATCATTGGAAAACAACCAGATTGATTTGGCTATCATGAATCTGCCAGATGAAACC
ATTAAGAACTGGAAACCATACGCCAGCAAGAAAATTATTGATGAACAATTGCTGTTCTTGCACCACGATGACGCCTTGGC
TAACCGTAAGCGAATCCATTTCAAAGATACTTTAGCTGAACCTTGGGCAACTTATCCTGACGACTATTTCCTTGGACAGC
TTCTGCGTGAGGCGTACAAGAATCAAATGGTTGATTTCCCGCACGTGGTCGCACATTTCACCACGCCATATCAGTTAACC
CAATTCGTTAACTTGTCAGGGGTCAACACGGCCTTACCAACCAGTTTTGTTCGGGCACGCGCTGATCAAATCAAGGCCTA
TGCTATTGCGTTTGATCCGGCCATTCGCTTTGAGATTTCTTTTGTTTATCGCAAAGAGAAAGCTGACATTCCGCGGATCT
CGAACTTCTTCCAGACCTTTGACGATTATCTGAAGGAAAAAGATTACTTGTCGCGGGTCAGTGAAAACCGTGTTAAACTT
GATAAGTAA

Upstream 100 bases:

>100_bases
CGATTAAAAACGCGGCAAATTCGTTGCGTTTTTTTTCATTGCCACTTATGATGATGGTTGAACGATATTATTAAATAATA
AACGAAAGGGAAGCCGCCCT

Downstream 100 bases:

>100_bases
ATTATTCAGTTGAGGAGCGATCAGATAAGATGACGACTTTGATTTTTGGGCATCAGAACCCGGACACCGACGCGATTACG
AGTGCCATGAGCTGGGCAGA

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MKTKQERIFSSKTLTYFLQLAETMNYTQAAQLLGITQPALTQQIKKLERTVGAPLFYSVGKKLHLSDAGQTMLKSTHEIY
EILNQTTDEIQQTTSATVGKINIGLLSSIEDSAFIAFIAHYYQLNPEVEVTLRMMTRHEIWESLENNQIDLAIMNLPDET
IKNWKPYASKKIIDEQLLFLHHDDALANRKRIHFKDTLAEPWATYPDDYFLGQLLREAYKNQMVDFPHVVAHFTTPYQLT
QFVNLSGVNTALPTSFVRARADQIKAYAIAFDPAIRFEISFVYRKEKADIPRISNFFQTFDDYLKEKDYLSRVSENRVKL
DK

Sequences:

>Translated_322_residues
MKTKQERIFSSKTLTYFLQLAETMNYTQAAQLLGITQPALTQQIKKLERTVGAPLFYSVGKKLHLSDAGQTMLKSTHEIY
EILNQTTDEIQQTTSATVGKINIGLLSSIEDSAFIAFIAHYYQLNPEVEVTLRMMTRHEIWESLENNQIDLAIMNLPDET
IKNWKPYASKKIIDEQLLFLHHDDALANRKRIHFKDTLAEPWATYPDDYFLGQLLREAYKNQMVDFPHVVAHFTTPYQLT
QFVNLSGVNTALPTSFVRARADQIKAYAIAFDPAIRFEISFVYRKEKADIPRISNFFQTFDDYLKEKDYLSRVSENRVKL
DK
>Mature_322_residues
MKTKQERIFSSKTLTYFLQLAETMNYTQAAQLLGITQPALTQQIKKLERTVGAPLFYSVGKKLHLSDAGQTMLKSTHEIY
EILNQTTDEIQQTTSATVGKINIGLLSSIEDSAFIAFIAHYYQLNPEVEVTLRMMTRHEIWESLENNQIDLAIMNLPDET
IKNWKPYASKKIIDEQLLFLHHDDALANRKRIHFKDTLAEPWATYPDDYFLGQLLREAYKNQMVDFPHVVAHFTTPYQLT
QFVNLSGVNTALPTSFVRARADQIKAYAIAFDPAIRFEISFVYRKEKADIPRISNFFQTFDDYLKEKDYLSRVSENRVKL
DK

Specific function: Positive Regulatory Protein For The Cyn Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=147, Percent_Identity=29.2517006802721, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787879, Length=296, Percent_Identity=24.6621621621622, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787601, Length=137, Percent_Identity=29.9270072992701, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1790399, Length=282, Percent_Identity=23.4042553191489, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI145693105, Length=305, Percent_Identity=22.6229508196721, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI2367136, Length=156, Percent_Identity=26.2820512820513, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 37307; Mature: 37307

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKQERIFSSKTLTYFLQLAETMNYTQAAQLLGITQPALTQQIKKLERTVGAPLFYSVG
CCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHCC
KKLHLSDAGQTMLKSTHEIYEILNQTTDEIQQTTSATVGKINIGLLSSIEDSAFIAFIAH
CEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEHHHHCCCHHHHHHHHHH
YYQLNPEVEVTLRMMTRHEIWESLENNQIDLAIMNLPDETIKNWKPYASKKIIDEQLLFL
HHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHCCCCHHHHHHHHHHHHHE
HHDDALANRKRIHFKDTLAEPWATYPDDYFLGQLLREAYKNQMVDFPHVVAHFTTPYQLT
ECCHHHHCCCCEEEHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHH
QFVNLSGVNTALPTSFVRARADQIKAYAIAFDPAIRFEISFVYRKEKADIPRISNFFQTF
HHHCCCCCCHHHHHHHHHHHHHHHEEEEEEECCEEEEEEEEEEECCCCCCCHHHHHHHHH
DDYLKEKDYLSRVSENRVKLDK
HHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKTKQERIFSSKTLTYFLQLAETMNYTQAAQLLGITQPALTQQIKKLERTVGAPLFYSVG
CCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHCC
KKLHLSDAGQTMLKSTHEIYEILNQTTDEIQQTTSATVGKINIGLLSSIEDSAFIAFIAH
CEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEHHHHCCCHHHHHHHHHH
YYQLNPEVEVTLRMMTRHEIWESLENNQIDLAIMNLPDETIKNWKPYASKKIIDEQLLFL
HHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHCCCCHHHHHHHHHHHHHE
HHDDALANRKRIHFKDTLAEPWATYPDDYFLGQLLREAYKNQMVDFPHVVAHFTTPYQLT
ECCHHHHCCCCEEEHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHH
QFVNLSGVNTALPTSFVRARADQIKAYAIAFDPAIRFEISFVYRKEKADIPRISNFFQTF
HHHCCCCCCHHHHHHHHHHHHHHHEEEEEEECCEEEEEEEEEEECCCCCCCHHHHHHHHH
DDYLKEKDYLSRVSENRVKLDK
HHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]