Click here to switch to the map view.

The map label for this gene is act [H]

Identifier: 116494898

GI number: 116494898

Start: 1403426

End: 1404277

Strand: Direct

Name: act [H]

Synonym: LSEI_1412

Alternate gene names: 116494898

Gene position: 1403426-1404277 (Clockwise)

Preceding gene: 116494897

Following gene: 116494899

Centisome position: 48.47

GC content: 48.0

Gene sequence:

>852_bases
ATGAAACTCATCGATCAGTCACAAAGCCCATTAAATATCTTAGAACAAGTTGGTCAGGATCATGATGGCCCGATCAAAGG
GTATGTTCACTCGGTCGAAAGTTTTGGTTCGGTCGATGGCCCTGGTATTCGCTTCGTTGTCTTTATGCAAGGGTGTCGCA
TGCGTTGCCAGTATTGTCACAACCCTGACACCTGGAACATTGGGGTTGGTGAAGAAATGACGGCCGATCAAATTTTGGCG
GATGCCCAGCGCTATAAAGCATTCTGGGGTGACCAAGGGGGTATTACGTGCAGTGGCGGTGAGAGTTTGGTACAAATCGA
TTTCATTCTCGAACTCTTCACCAAGGCTAAGGCACTGGGAATTTCGACTTGCCTCGATACGTCAGGCGGCCCCTTCACGC
GTGACCAACCGTGGTTTGGCCAGTTTGAAAAGCTGATGGCTGTTACTGACATCTCATTAGTTGATATTAAACACATCGAT
TCGGCCGAGCATAAAAAGCTCACCGGTTTTCCTAATGAAAATATTTTAGATATGGTGCAGTATATGTCGGCGCATGGTGA
TGACATGTGGATTCGCCACGTTCTGGTCCCGGAACGCACTGACTTTGACCCTTATCTCAAACGATTAGGGGACTATATTG
CGACATTGGACAAAAACGTGGTCCAAAAAGTTGAGATTCTGCCGTATCACACGTTAGGCGTTAAAAAGTATCACGAGCTT
GGCATTACGTACCCGCTTGAAGGCATCGAACCGCCGTCTGCCGATCGCGTTAAGAATGCAGAGAATCTGCTGCACGTTAA
GGATTATACCGGATGGCAAAGCTGGCGTCCGAAACCAGTTGCGAGCAACTGA

Upstream 100 bases:

>100_bases
GTTTTGCCAAAAGACGGCAACGATGCTGTTGCCGTTTTTTGGCAGAATGAAATGAAAATCAATTGAAAACGACACATGAT
GTTGTAGTGGAGGCAATAAG

Downstream 100 bases:

>100_bases
ATTTTTAGCTAGAACTTAACGTAACGATTAAAAACGCGGCAAATTCGTTGCGTTTTTTTTCATTGCCACTTATGATGATG
GTTGAACGATATTATTAAAT

Product: pyruvate-formate lyase-activating enzyme

Products: NA

Alternate protein names: PFL-activating enzyme; Formate-C-acetyltransferase-activating enzyme [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MKLIDQSQSPLNILEQVGQDHDGPIKGYVHSVESFGSVDGPGIRFVVFMQGCRMRCQYCHNPDTWNIGVGEEMTADQILA
DAQRYKAFWGDQGGITCSGGESLVQIDFILELFTKAKALGISTCLDTSGGPFTRDQPWFGQFEKLMAVTDISLVDIKHID
SAEHKKLTGFPNENILDMVQYMSAHGDDMWIRHVLVPERTDFDPYLKRLGDYIATLDKNVVQKVEILPYHTLGVKKYHEL
GITYPLEGIEPPSADRVKNAENLLHVKDYTGWQSWRPKPVASN

Sequences:

>Translated_283_residues
MKLIDQSQSPLNILEQVGQDHDGPIKGYVHSVESFGSVDGPGIRFVVFMQGCRMRCQYCHNPDTWNIGVGEEMTADQILA
DAQRYKAFWGDQGGITCSGGESLVQIDFILELFTKAKALGISTCLDTSGGPFTRDQPWFGQFEKLMAVTDISLVDIKHID
SAEHKKLTGFPNENILDMVQYMSAHGDDMWIRHVLVPERTDFDPYLKRLGDYIATLDKNVVQKVEILPYHTLGVKKYHEL
GITYPLEGIEPPSADRVKNAENLLHVKDYTGWQSWRPKPVASN
>Mature_283_residues
MKLIDQSQSPLNILEQVGQDHDGPIKGYVHSVESFGSVDGPGIRFVVFMQGCRMRCQYCHNPDTWNIGVGEEMTADQILA
DAQRYKAFWGDQGGITCSGGESLVQIDFILELFTKAKALGISTCLDTSGGPFTRDQPWFGQFEKLMAVTDISLVDIKHID
SAEHKKLTGFPNENILDMVQYMSAHGDDMWIRHVLVPERTDFDPYLKRLGDYIATLDKNVVQKVEILPYHTLGVKKYHEL
GITYPLEGIEPPSADRVKNAENLLHVKDYTGWQSWRPKPVASN

Specific function: Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=242, Percent_Identity=39.6694214876033, Blast_Score=194, Evalue=8e-51,
Organism=Escherichia coli, GI1790839, Length=287, Percent_Identity=27.1777003484321, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790389, Length=269, Percent_Identity=21.9330855018587, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI226510931, Length=275, Percent_Identity=25.0909090909091, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 31861; Mature: 31861

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIDQSQSPLNILEQVGQDHDGPIKGYVHSVESFGSVDGPGIRFVVFMQGCRMRCQYCH
CCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHCC
NPDTWNIGVGEEMTADQILADAQRYKAFWGDQGGITCSGGESLVQIDFILELFTKAKALG
CCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEEHHHHHHHHHHHHHHHH
ISTCLDTSGGPFTRDQPWFGQFEKLMAVTDISLVDIKHIDSAEHKKLTGFPNENILDMVQ
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEHHHCCCCCCCCCCCCCCCHHHHHHH
YMSAHGDDMWIRHVLVPERTDFDPYLKRLGDYIATLDKNVVQKVEILPYHTLGVKKYHEL
HHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCHHHHHHC
GITYPLEGIEPPSADRVKNAENLLHVKDYTGWQSWRPKPVASN
CCCCCCCCCCCCCHHHHCCHHHEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKLIDQSQSPLNILEQVGQDHDGPIKGYVHSVESFGSVDGPGIRFVVFMQGCRMRCQYCH
CCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHCC
NPDTWNIGVGEEMTADQILADAQRYKAFWGDQGGITCSGGESLVQIDFILELFTKAKALG
CCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEEHHHHHHHHHHHHHHHH
ISTCLDTSGGPFTRDQPWFGQFEKLMAVTDISLVDIKHIDSAEHKKLTGFPNENILDMVQ
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEHHHCCCCCCCCCCCCCCCHHHHHHH
YMSAHGDDMWIRHVLVPERTDFDPYLKRLGDYIATLDKNVVQKVEILPYHTLGVKKYHEL
HHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCHHHHHHC
GITYPLEGIEPPSADRVKNAENLLHVKDYTGWQSWRPKPVASN
CCCCCCCCCCCCCHHHHCCHHHEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11029425; 10899886; 12397186 [H]