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The map label for this gene is pflB [H]

Identifier: 116494896

GI number: 116494896

Start: 1401033

End: 1403294

Strand: Direct

Name: pflB [H]

Synonym: LSEI_1410

Alternate gene names: 116494896

Gene position: 1401033-1403294 (Clockwise)

Preceding gene: 116494895

Following gene: 116494897

Centisome position: 48.39

GC content: 46.2

Gene sequence:

>2262_bases
ATGAAACAGCTTGACGAAACCAAAGTTCCTAATTATTGGGAAGGCTTTAACGGCGGCGACTGGCAAGAAGAAATTAATGT
GCGCGATTTTATCGAGCATAACCTGAACCAATATGACGGGGACGAAAGTTTCCTTGCTGGTCCCACTGAAGCGACAACAG
TCCTGAACAATCAGGTTCTAAATTTAAAGAAGCAAGAACGTGCAAATGGCGGTGTTTTGGATGCTGACAACAATATTCCA
TCTACGATCACCTCACACGGCCCTGGTTATTTGAACAAAGATCTTGAAAAGATTGTTGGTGTTCAGACCGACAAGCCATT
CAAACGAGCATTCATGCCATTTGGCGGTATTCGAATGGCTGAAGATGCATTGGAATCTTATGGCTTCAAGACAGATCCTC
AAGAACACAAGATTTTCAATGAATATCGTAAGACCCATAACCAAGGTGTCTTTGATGCTTATACCCCTGATATGCGAAAG
GCTCGCCATTACAAGATCGTGACTGGCTTGCCAGACGCATACGGCCGCGGCCGGATTGTTTCTGATTTCCCACGGATCGC
TGTTTACGGGATCGATCGTTTAATGGCTGAGAAGTTCAAGGATTATAACTTGACCGGCGACGGCGAAATGACTGACGATG
TAATCAAATTGCGTGAAGAAATCAATGAACAATATCGTGCCTTAAACGACATGAAGAAGATGGCCAAGGAATATGGCTAT
GACATCTCGCGTCCGGCAGCTAATGCGCAAGAAGCTGTTCAGTGGATCTACTTTGGCTATCTGGCTGCTGTTAAGACTCA
AAACGGTGCCGCAATGTCCGTTGGCCGGATCGATACGGTTATTGATGCTTATATCCAACGCGATATGCGCTTAGGCAAAT
TGAACGAAGAACAGGCACAGGAACTGATTGATCATTTGGTTATGAAATTGCGGATGGTTCGTTTCATCCGGACTGAAGAA
TATAACTCACTGTTCTCAGGCGATCCAATCTGGGCAACCTTGTCATTGGCAGGAATGGGCAATGATGGCCGTCACCACGT
TACCAAGACGGCTTTCCGTTTCCTGAAGACTTTGGACAACATGGGCGCAGCACCAGAACCAAACATCACCTTGCTTTGGA
GCGAACGCTTGCCAGAAGGCTTTAAACGCTATGCAACCGAAGTATCCATCCAAAGTTCAACCATTCAATATGAAAATGAT
GACCTGATGCGCAATGAATGGGGTACCGATTACTACGGTATTGCTTGCTGTGTCTCGGCACAACCAATTGCTGATGGCGT
TCAATACTTTGGTGCCCGTGCTAACTTAGCTAAGACGGTTCTGTATGCGATCAACGGTGGTAAAGATGAAATTCAGGAAG
CGCAAGTAGGGCCTGAATATGCACCGATCACCAGCGATTACATTGATTACAAAGAATTCATGAATAAGTTCGACAAGATG
ATGGACTGGCTGGCCGACACGTATGTCAATGCTTTGAATGTGATTCACTATATGCATGACAAGTATTATTATGAAGCTGC
ACAGCTGGCATTGAAGGATACCCAACTGAACCGGACCTTCGCAACCGGGATTTCCGGCCTGTCACACGCGGTTGATTCCA
TCAGTGCAATCAAGTATGGTCATGTCAAGGCAATTCGCGATGAAAATGGTGTTGCAGTTGACTTCGTTGCTGACAATGAT
GACTATCCACGTTATGGTAACAACGATGATCGCGCTGATGACATCGCTAAGTGGTTGGTTAAGACCTTCTATAACAAGAT
GAACACGCATCATCTGTATCGCGGCGCGAAGTTGAGTACCAGTGTCCTGACCATTACTTCCAACGTGGTTTATGGTAAGA
ATACAGGGACAACACCAAACGGTCGTCAAAAAGGCGAACCATTCTCACCAGGTGCCAACCCGGCATACGGCGCAGAAAAG
AACGGCGCATTGGCATCCTTGATGTCCACCGCCAAGATTCCGTATCATTACGCAACTGACGGCATTAGTAATACGTTTGG
GGTTACTCCGAACACACTGGGCCACGATGATGAAACTCGTAAGGATACCTTGGTTCACATGGTTGACGGCTATATGGAAA
ACAGCGGCATGCATTTGAACATCAACGTCTTCAACAAGGAGACCCTGATTGATGCTCAGAAGCACCCAGAAGAATACCCA
ACATTGACCGTTCGGGTTTCTGGCTACTGCGTCTACTTCGCAGATTTGACCAAGGAACAACAAGACGATGTCATTGCTCG
GACCTTCTTCGAAGAAATGTAA

Upstream 100 bases:

>100_bases
ATTATATGGGCAATAAAGCGCTTGCATAGATTTGGCGGCGTGTTATACTGTGTTTGTAAGAACTGTCACATCTGTAATCC
ACTTTAGGAGGCAAACAACT

Downstream 100 bases:

>100_bases
GCAGCTTAACTCAAGACAGGAAGCCATTCCTGTTTTGCCAAAAGACGGCAACGATGCTGTTGCCGTTTTTTGGCAGAATG
AAATGAAAATCAATTGAAAA

Product: pyruvate-formate lyase

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 753; Mature: 753

Protein sequence:

>753_residues
MKQLDETKVPNYWEGFNGGDWQEEINVRDFIEHNLNQYDGDESFLAGPTEATTVLNNQVLNLKKQERANGGVLDADNNIP
STITSHGPGYLNKDLEKIVGVQTDKPFKRAFMPFGGIRMAEDALESYGFKTDPQEHKIFNEYRKTHNQGVFDAYTPDMRK
ARHYKIVTGLPDAYGRGRIVSDFPRIAVYGIDRLMAEKFKDYNLTGDGEMTDDVIKLREEINEQYRALNDMKKMAKEYGY
DISRPAANAQEAVQWIYFGYLAAVKTQNGAAMSVGRIDTVIDAYIQRDMRLGKLNEEQAQELIDHLVMKLRMVRFIRTEE
YNSLFSGDPIWATLSLAGMGNDGRHHVTKTAFRFLKTLDNMGAAPEPNITLLWSERLPEGFKRYATEVSIQSSTIQYEND
DLMRNEWGTDYYGIACCVSAQPIADGVQYFGARANLAKTVLYAINGGKDEIQEAQVGPEYAPITSDYIDYKEFMNKFDKM
MDWLADTYVNALNVIHYMHDKYYYEAAQLALKDTQLNRTFATGISGLSHAVDSISAIKYGHVKAIRDENGVAVDFVADND
DYPRYGNNDDRADDIAKWLVKTFYNKMNTHHLYRGAKLSTSVLTITSNVVYGKNTGTTPNGRQKGEPFSPGANPAYGAEK
NGALASLMSTAKIPYHYATDGISNTFGVTPNTLGHDDETRKDTLVHMVDGYMENSGMHLNINVFNKETLIDAQKHPEEYP
TLTVRVSGYCVYFADLTKEQQDDVIARTFFEEM

Sequences:

>Translated_753_residues
MKQLDETKVPNYWEGFNGGDWQEEINVRDFIEHNLNQYDGDESFLAGPTEATTVLNNQVLNLKKQERANGGVLDADNNIP
STITSHGPGYLNKDLEKIVGVQTDKPFKRAFMPFGGIRMAEDALESYGFKTDPQEHKIFNEYRKTHNQGVFDAYTPDMRK
ARHYKIVTGLPDAYGRGRIVSDFPRIAVYGIDRLMAEKFKDYNLTGDGEMTDDVIKLREEINEQYRALNDMKKMAKEYGY
DISRPAANAQEAVQWIYFGYLAAVKTQNGAAMSVGRIDTVIDAYIQRDMRLGKLNEEQAQELIDHLVMKLRMVRFIRTEE
YNSLFSGDPIWATLSLAGMGNDGRHHVTKTAFRFLKTLDNMGAAPEPNITLLWSERLPEGFKRYATEVSIQSSTIQYEND
DLMRNEWGTDYYGIACCVSAQPIADGVQYFGARANLAKTVLYAINGGKDEIQEAQVGPEYAPITSDYIDYKEFMNKFDKM
MDWLADTYVNALNVIHYMHDKYYYEAAQLALKDTQLNRTFATGISGLSHAVDSISAIKYGHVKAIRDENGVAVDFVADND
DYPRYGNNDDRADDIAKWLVKTFYNKMNTHHLYRGAKLSTSVLTITSNVVYGKNTGTTPNGRQKGEPFSPGANPAYGAEK
NGALASLMSTAKIPYHYATDGISNTFGVTPNTLGHDDETRKDTLVHMVDGYMENSGMHLNINVFNKETLIDAQKHPEEYP
TLTVRVSGYCVYFADLTKEQQDDVIARTFFEEM
>Mature_753_residues
MKQLDETKVPNYWEGFNGGDWQEEINVRDFIEHNLNQYDGDESFLAGPTEATTVLNNQVLNLKKQERANGGVLDADNNIP
STITSHGPGYLNKDLEKIVGVQTDKPFKRAFMPFGGIRMAEDALESYGFKTDPQEHKIFNEYRKTHNQGVFDAYTPDMRK
ARHYKIVTGLPDAYGRGRIVSDFPRIAVYGIDRLMAEKFKDYNLTGDGEMTDDVIKLREEINEQYRALNDMKKMAKEYGY
DISRPAANAQEAVQWIYFGYLAAVKTQNGAAMSVGRIDTVIDAYIQRDMRLGKLNEEQAQELIDHLVMKLRMVRFIRTEE
YNSLFSGDPIWATLSLAGMGNDGRHHVTKTAFRFLKTLDNMGAAPEPNITLLWSERLPEGFKRYATEVSIQSSTIQYEND
DLMRNEWGTDYYGIACCVSAQPIADGVQYFGARANLAKTVLYAINGGKDEIQEAQVGPEYAPITSDYIDYKEFMNKFDKM
MDWLADTYVNALNVIHYMHDKYYYEAAQLALKDTQLNRTFATGISGLSHAVDSISAIKYGHVKAIRDENGVAVDFVADND
DYPRYGNNDDRADDIAKWLVKTFYNKMNTHHLYRGAKLSTSVLTITSNVVYGKNTGTTPNGRQKGEPFSPGANPAYGAEK
NGALASLMSTAKIPYHYATDGISNTFGVTPNTLGHDDETRKDTLVHMVDGYMENSGMHLNINVFNKETLIDAQKHPEEYP
TLTVRVSGYCVYFADLTKEQQDDVIARTFFEEM

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=762, Percent_Identity=58.9238845144357, Blast_Score=927, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=750, Percent_Identity=57.2, Blast_Score=885, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=652, Percent_Identity=24.3865030674847, Blast_Score=172, Evalue=6e-44,
Organism=Escherichia coli, GI1790388, Length=188, Percent_Identity=32.4468085106383, Blast_Score=109, Evalue=7e-25,
Organism=Escherichia coli, GI1788933, Length=58, Percent_Identity=67.2413793103448, Blast_Score=85, Evalue=2e-17,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 85144; Mature: 85144

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQLDETKVPNYWEGFNGGDWQEEINVRDFIEHNLNQYDGDESFLAGPTEATTVLNNQVL
CCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHHH
NLKKQERANGGVLDADNNIPSTITSHGPGYLNKDLEKIVGVQTDKPFKRAFMPFGGIRMA
CCHHHHCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHCCCCCCHHH
EDALESYGFKTDPQEHKIFNEYRKTHNQGVFDAYTPDMRKARHYKIVTGLPDAYGRGRIV
HHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCEEEEECCCCCCCCCCEE
SDFPRIAVYGIDRLMAEKFKDYNLTGDGEMTDDVIKLREEINEQYRALNDMKKMAKEYGY
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DISRPAANAQEAVQWIYFGYLAAVKTQNGAAMSVGRIDTVIDAYIQRDMRLGKLNEEQAQ
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCHHHHH
ELIDHLVMKLRMVRFIRTEEYNSLFSGDPIWATLSLAGMGNDGRHHVTKTAFRFLKTLDN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHH
MGAAPEPNITLLWSERLPEGFKRYATEVSIQSSTIQYENDDLMRNEWGTDYYGIACCVSA
CCCCCCCCEEEEECHHCCHHHHHHHHHHEEECCEEEECCCCHHHHCCCCCCEEEEEEECC
QPIADGVQYFGARANLAKTVLYAINGGKDEIQEAQVGPEYAPITSDYIDYKEFMNKFDKM
CHHHHHHHHHCCHHHHHHHHHEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
MDWLADTYVNALNVIHYMHDKYYYEAAQLALKDTQLNRTFATGISGLSHAVDSISAIKYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
HVKAIRDENGVAVDFVADNDDYPRYGNNDDRADDIAKWLVKTFYNKMNTHHLYRGAKLST
CEEEEECCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHCCCCCCC
SVLTITSNVVYGKNTGTTPNGRQKGEPFSPGANPAYGAEKNGALASLMSTAKIPYHYATD
EEEEEEECEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEECC
GISNTFGVTPNTLGHDDETRKDTLVHMVDGYMENSGMHLNINVFNKETLIDAQKHPEEYP
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHCCHHCCCCCC
TLTVRVSGYCVYFADLTKEQQDDVIARTFFEEM
EEEEEECCEEEEEEHHCCCHHHHHHHHHHHHHC
>Mature Secondary Structure
MKQLDETKVPNYWEGFNGGDWQEEINVRDFIEHNLNQYDGDESFLAGPTEATTVLNNQVL
CCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHHH
NLKKQERANGGVLDADNNIPSTITSHGPGYLNKDLEKIVGVQTDKPFKRAFMPFGGIRMA
CCHHHHCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHCCCCCCHHH
EDALESYGFKTDPQEHKIFNEYRKTHNQGVFDAYTPDMRKARHYKIVTGLPDAYGRGRIV
HHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCEEEEECCCCCCCCCCEE
SDFPRIAVYGIDRLMAEKFKDYNLTGDGEMTDDVIKLREEINEQYRALNDMKKMAKEYGY
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DISRPAANAQEAVQWIYFGYLAAVKTQNGAAMSVGRIDTVIDAYIQRDMRLGKLNEEQAQ
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCHHHHH
ELIDHLVMKLRMVRFIRTEEYNSLFSGDPIWATLSLAGMGNDGRHHVTKTAFRFLKTLDN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHH
MGAAPEPNITLLWSERLPEGFKRYATEVSIQSSTIQYENDDLMRNEWGTDYYGIACCVSA
CCCCCCCCEEEEECHHCCHHHHHHHHHHEEECCEEEECCCCHHHHCCCCCCEEEEEEECC
QPIADGVQYFGARANLAKTVLYAINGGKDEIQEAQVGPEYAPITSDYIDYKEFMNKFDKM
CHHHHHHHHHCCHHHHHHHHHEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
MDWLADTYVNALNVIHYMHDKYYYEAAQLALKDTQLNRTFATGISGLSHAVDSISAIKYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
HVKAIRDENGVAVDFVADNDDYPRYGNNDDRADDIAKWLVKTFYNKMNTHHLYRGAKLST
CEEEEECCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHCCCCCCC
SVLTITSNVVYGKNTGTTPNGRQKGEPFSPGANPAYGAEKNGALASLMSTAKIPYHYATD
EEEEEEECEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEECC
GISNTFGVTPNTLGHDDETRKDTLVHMVDGYMENSGMHLNINVFNKETLIDAQKHPEEYP
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHCCHHCCCCCC
TLTVRVSGYCVYFADLTKEQQDDVIARTFFEEM
EEEEEECCEEEEEEHHCCCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA