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The map label for this gene is topA [H]

Identifier: 116494887

GI number: 116494887

Start: 1387071

End: 1389155

Strand: Direct

Name: topA [H]

Synonym: LSEI_1401

Alternate gene names: 116494887

Gene position: 1387071-1389155 (Clockwise)

Preceding gene: 116494886

Following gene: 116494888

Centisome position: 47.91

GC content: 46.52

Gene sequence:

>2085_bases
ATGGCCGACAAGAAGTTAGTCATTGTTGAATCGCCAGCCAAAGCCAAGACTATTGAAAAGTATCTTGGCCGAACCTATAA
AGTTGTTGCAAGTTTGGGGCACATTCGCGATCTACCTAAGAGTCAGATGGGGGTCGATGTCGATAACGACTACGAGCCAA
AGTACATTTCGATTCGCGGCAAAGGCGATGTGATTAAATCCCTTAAAAAAGATGCTAAAAAAGCTAGCAAGATTTATCTC
GCAGCCGATCCGGATCGTGAAGGAGAAGCCATTGCATGGCATGTCAGTCACATTTTAGGTTTGGATCCAAAAGCCAAAAA
TCGCGTCGTTTTTAACGAAATTACGAAAGATGCCGTTAAAAATGCGTTCAAAACCCCTCGTGGCATTGATATGGATCTTG
TAGATGCCCAGCAGGCACGGCGGATTTTGGATCGACTGGTAGGCTATTCCATCAGTCCTTTATTATGGAAGAAAGTTAAA
AAGGGCCTGAGTGCTGGCCGCGTTCAAAGTGTTGCGCTAAAGCTGATTATTGATCGCGAAAATGAAATCAAAAACTTCAC
GCCAGAAGAATACTGGTCATTAGATGCTGAGTTCCAAAAGGGCCGTAGTAAAAAGTTCAGTGCTAGTTTCTATGGCCTGA
AGAATAAAAAGCAGGCTTTAAAGAACAACGAGGCCGTTCAAAAGATTTTGGGTCAAGTGGACAAAACTAAGCCGTTTGCT
GTGACGAATGTTGTTAAAAAGGAACGCAAACGCTTCCCATCCCCGCCGTTCACAACCAGTACCTTGCAGCAGGAAGCTAA
CCGTAAGTTGGGATTCCGCACCCGAAAGACCATGATGTTGGCCCAGCAACTGTATGAAGGAATTAACTTAGGTGGTAAGT
TAGGCACCGTTGGGCTGATCACGTACATGCGTACCGACTCGACTCGCGTGTCAAATGTTGCCAAAACTGAAACCAGCCAT
TACCTGCATGAGAAATACGGCGAAGCTTATGCTGCTGTCAAAATTCGCCAAGGTAAACTGCAGGAAGGGGCACAAGATGC
CCACGAAGCCATTCGGCCAACCAGCACATTACGGACACCGGATAGTTTGACGGCAGTTTTGGACAAGGATCAGATGAAAC
TTTATCGGCTGATCTGGAGTCGGTTTGTTGCCAGTGAAATGACCCCGGCGATTATTGACACTGTGGCCGTGACGTTAGAC
CAAAACGGTGTGACTTTCCGCGCCAACGGTAGTCAGACTAAGTTTGAAGGGTTTACCAAGTTGTATGTCTCATCACGTGA
CAGCGATCAAAAGGACAATATTTTGCCACCGCTTGCAGTTGGTGATGATGTCAAAATGTTGAAGACCGATCCGGCTCAAC
ACTTTACACAGCCACCAGCCCGTTACTCGGAAGCAAACCTTGTCAAAGCGCTTGAAGAAAATGGCGTTGGTCGTCCGTCA
ACTTATGCGCCAACTATCGAAACCATTCAGAAACGTTACTACGTGAAACTGAATGCTCGTCGGTTTGAACCGACTGAATT
AGGTGAGATCGTCAACAGCTTGATCGTTGAATTCTTCCCGGACATCGTGAATGTCAAGTTTACGGCGAACGTCGAATCTG
AACTTGATAGGATTGAAACCGGCGAGGCTGATTGGGTTAAGGTGATTGATCAGTTCTATAAGCCATTTGAAAAAGAAGTG
GCTCACGCCGAAACTGGGATCGCGAAGATTCAAATCAAAGACGAACCAGCCGGCTTCAACTGTGACATCTGCGGTGCGCC
AATGGTTATCAAAATGGGTCGCTATGGTAAGTTTTACGCCTGTTCCCGATTCCCGAATTGCCGCAACACCAAGGCCATTG
TCAAAGAGATCGGGGTTGTCTGCCCGAAATGTCATATCGGTCAAGTTGTTGAACGTAAGAGCAAGCGTAACCGCGTTTTT
TATGGTTGCTCGCGTTATCCAGACTGCGACTTTGTCTCATGGGACAAACCAGTCGGCCGCGATTGCCCGGTCTGCGGACA
TTTCTTAGTTGAGAAAAAGAGCAAAGCTGGCACCCAAGTTGTCTGCCCAAATAACGATTATAAAGAAGCCTTACAAAAAG
CATAA

Upstream 100 bases:

>100_bases
GCCGCCTTAGGACGCATCAATTACTGACGACGAATCGTCAGCATCGCACAAATTAATCAGGGCTTGCCTACTAGGCAATG
CCACCGGAAGGAGCGCGTTC

Downstream 100 bases:

>100_bases
TTTGAGAAAGCAGTGAGTATGTTATCTTTGTACTCACTGTTTTTTTCGGGTAAACTTGGGCAGAGCGCGAACAGGCGCGC
TTAGAAACCGGAGCCTAAAT

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 694; Mature: 693

Protein sequence:

>694_residues
MADKKLVIVESPAKAKTIEKYLGRTYKVVASLGHIRDLPKSQMGVDVDNDYEPKYISIRGKGDVIKSLKKDAKKASKIYL
AADPDREGEAIAWHVSHILGLDPKAKNRVVFNEITKDAVKNAFKTPRGIDMDLVDAQQARRILDRLVGYSISPLLWKKVK
KGLSAGRVQSVALKLIIDRENEIKNFTPEEYWSLDAEFQKGRSKKFSASFYGLKNKKQALKNNEAVQKILGQVDKTKPFA
VTNVVKKERKRFPSPPFTTSTLQQEANRKLGFRTRKTMMLAQQLYEGINLGGKLGTVGLITYMRTDSTRVSNVAKTETSH
YLHEKYGEAYAAVKIRQGKLQEGAQDAHEAIRPTSTLRTPDSLTAVLDKDQMKLYRLIWSRFVASEMTPAIIDTVAVTLD
QNGVTFRANGSQTKFEGFTKLYVSSRDSDQKDNILPPLAVGDDVKMLKTDPAQHFTQPPARYSEANLVKALEENGVGRPS
TYAPTIETIQKRYYVKLNARRFEPTELGEIVNSLIVEFFPDIVNVKFTANVESELDRIETGEADWVKVIDQFYKPFEKEV
AHAETGIAKIQIKDEPAGFNCDICGAPMVIKMGRYGKFYACSRFPNCRNTKAIVKEIGVVCPKCHIGQVVERKSKRNRVF
YGCSRYPDCDFVSWDKPVGRDCPVCGHFLVEKKSKAGTQVVCPNNDYKEALQKA

Sequences:

>Translated_694_residues
MADKKLVIVESPAKAKTIEKYLGRTYKVVASLGHIRDLPKSQMGVDVDNDYEPKYISIRGKGDVIKSLKKDAKKASKIYL
AADPDREGEAIAWHVSHILGLDPKAKNRVVFNEITKDAVKNAFKTPRGIDMDLVDAQQARRILDRLVGYSISPLLWKKVK
KGLSAGRVQSVALKLIIDRENEIKNFTPEEYWSLDAEFQKGRSKKFSASFYGLKNKKQALKNNEAVQKILGQVDKTKPFA
VTNVVKKERKRFPSPPFTTSTLQQEANRKLGFRTRKTMMLAQQLYEGINLGGKLGTVGLITYMRTDSTRVSNVAKTETSH
YLHEKYGEAYAAVKIRQGKLQEGAQDAHEAIRPTSTLRTPDSLTAVLDKDQMKLYRLIWSRFVASEMTPAIIDTVAVTLD
QNGVTFRANGSQTKFEGFTKLYVSSRDSDQKDNILPPLAVGDDVKMLKTDPAQHFTQPPARYSEANLVKALEENGVGRPS
TYAPTIETIQKRYYVKLNARRFEPTELGEIVNSLIVEFFPDIVNVKFTANVESELDRIETGEADWVKVIDQFYKPFEKEV
AHAETGIAKIQIKDEPAGFNCDICGAPMVIKMGRYGKFYACSRFPNCRNTKAIVKEIGVVCPKCHIGQVVERKSKRNRVF
YGCSRYPDCDFVSWDKPVGRDCPVCGHFLVEKKSKAGTQVVCPNNDYKEALQKA
>Mature_693_residues
ADKKLVIVESPAKAKTIEKYLGRTYKVVASLGHIRDLPKSQMGVDVDNDYEPKYISIRGKGDVIKSLKKDAKKASKIYLA
ADPDREGEAIAWHVSHILGLDPKAKNRVVFNEITKDAVKNAFKTPRGIDMDLVDAQQARRILDRLVGYSISPLLWKKVKK
GLSAGRVQSVALKLIIDRENEIKNFTPEEYWSLDAEFQKGRSKKFSASFYGLKNKKQALKNNEAVQKILGQVDKTKPFAV
TNVVKKERKRFPSPPFTTSTLQQEANRKLGFRTRKTMMLAQQLYEGINLGGKLGTVGLITYMRTDSTRVSNVAKTETSHY
LHEKYGEAYAAVKIRQGKLQEGAQDAHEAIRPTSTLRTPDSLTAVLDKDQMKLYRLIWSRFVASEMTPAIIDTVAVTLDQ
NGVTFRANGSQTKFEGFTKLYVSSRDSDQKDNILPPLAVGDDVKMLKTDPAQHFTQPPARYSEANLVKALEENGVGRPST
YAPTIETIQKRYYVKLNARRFEPTELGEIVNSLIVEFFPDIVNVKFTANVESELDRIETGEADWVKVIDQFYKPFEKEVA
HAETGIAKIQIKDEPAGFNCDICGAPMVIKMGRYGKFYACSRFPNCRNTKAIVKEIGVVCPKCHIGQVVERKSKRNRVFY
GCSRYPDCDFVSWDKPVGRDCPVCGHFLVEKKSKAGTQVVCPNNDYKEALQKA

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=625, Percent_Identity=25.6, Blast_Score=136, Evalue=6e-32,
Organism=Homo sapiens, GI4507635, Length=539, Percent_Identity=24.6753246753247, Blast_Score=112, Evalue=8e-25,
Organism=Escherichia coli, GI1787529, Length=753, Percent_Identity=39.1766268260292, Blast_Score=504, Evalue=1e-144,
Organism=Escherichia coli, GI1788061, Length=603, Percent_Identity=24.0464344941957, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI48994931, Length=105, Percent_Identity=39.0476190476191, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI32563869, Length=554, Percent_Identity=24.9097472924188, Blast_Score=125, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17555378, Length=448, Percent_Identity=23.8839285714286, Blast_Score=103, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6323263, Length=561, Percent_Identity=22.8163992869875, Blast_Score=103, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24585251, Length=659, Percent_Identity=23.8239757207891, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24640096, Length=526, Percent_Identity=23.9543726235741, Blast_Score=108, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 78081; Mature: 77949

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADKKLVIVESPAKAKTIEKYLGRTYKVVASLGHIRDLPKSQMGVDVDNDYEPKYISIRG
CCCCEEEEEECCCHHHHHHHHHCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEEEEC
KGDVIKSLKKDAKKASKIYLAADPDREGEAIAWHVSHILGLDPKAKNRVVFNEITKDAVK
CHHHHHHHHHHHHHHCEEEEEECCCCCCCEEEEEHHHHHCCCCCCCCCHHHHHHHHHHHH
NAFKTPRGIDMDLVDAQQARRILDRLVGYSISPLLWKKVKKGLSAGRVQSVALKLIIDRE
HHHCCCCCCCCHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHEEEEEEECCC
NEIKNFTPEEYWSLDAEFQKGRSKKFSASFYGLKNKKQALKNNEAVQKILGQVDKTKPFA
HHCCCCCCHHHHCCCHHHHCCCCCCCCHHHHCCCHHHHHHHCHHHHHHHHHHHCCCCCHH
VTNVVKKERKRFPSPPFTTSTLQQEANRKLGFRTRKTMMLAQQLYEGINLGGKLGTVGLI
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHEE
TYMRTDSTRVSNVAKTETSHYLHEKYGEAYAAVKIRQGKLQEGAQDAHEAIRPTSTLRTP
EEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCC
DSLTAVLDKDQMKLYRLIWSRFVASEMTPAIIDTVAVTLDQNGVTFRANGSQTKFEGFTK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEEEEECCCCEEEEECCCCCHHCCCEE
LYVSSRDSDQKDNILPPLAVGDDVKMLKTDPAQHFTQPPARYSEANLVKALEENGVGRPS
EEEECCCCCCCCCCCCCCCCCCCCCEEECCCHHHCCCCCCCCCHHHHHHHHHHCCCCCCC
TYAPTIETIQKRYYVKLNARRFEPTELGEIVNSLIVEFFPDIVNVKFTANVESELDRIET
CCCCHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCEEEEECHHHHHHHHCC
GEADWVKVIDQFYKPFEKEVAHAETGIAKIQIKDEPAGFNCDICGAPMVIKMGRYGKFYA
CCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEE
CSRFPNCRNTKAIVKEIGVVCPKCHIGQVVERKSKRNRVFYGCSRYPDCDFVSWDKPVGR
ECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCC
DCPVCGHFLVEKKSKAGTQVVCPNNDYKEALQKA
CCCHHHHHHHHHHCCCCCEEECCCCHHHHHHHCC
>Mature Secondary Structure 
ADKKLVIVESPAKAKTIEKYLGRTYKVVASLGHIRDLPKSQMGVDVDNDYEPKYISIRG
CCCEEEEEECCCHHHHHHHHHCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEEEEC
KGDVIKSLKKDAKKASKIYLAADPDREGEAIAWHVSHILGLDPKAKNRVVFNEITKDAVK
CHHHHHHHHHHHHHHCEEEEEECCCCCCCEEEEEHHHHHCCCCCCCCCHHHHHHHHHHHH
NAFKTPRGIDMDLVDAQQARRILDRLVGYSISPLLWKKVKKGLSAGRVQSVALKLIIDRE
HHHCCCCCCCCHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHEEEEEEECCC
NEIKNFTPEEYWSLDAEFQKGRSKKFSASFYGLKNKKQALKNNEAVQKILGQVDKTKPFA
HHCCCCCCHHHHCCCHHHHCCCCCCCCHHHHCCCHHHHHHHCHHHHHHHHHHHCCCCCHH
VTNVVKKERKRFPSPPFTTSTLQQEANRKLGFRTRKTMMLAQQLYEGINLGGKLGTVGLI
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHEE
TYMRTDSTRVSNVAKTETSHYLHEKYGEAYAAVKIRQGKLQEGAQDAHEAIRPTSTLRTP
EEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCC
DSLTAVLDKDQMKLYRLIWSRFVASEMTPAIIDTVAVTLDQNGVTFRANGSQTKFEGFTK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEEEEECCCCEEEEECCCCCHHCCCEE
LYVSSRDSDQKDNILPPLAVGDDVKMLKTDPAQHFTQPPARYSEANLVKALEENGVGRPS
EEEECCCCCCCCCCCCCCCCCCCCCEEECCCHHHCCCCCCCCCHHHHHHHHHHCCCCCCC
TYAPTIETIQKRYYVKLNARRFEPTELGEIVNSLIVEFFPDIVNVKFTANVESELDRIET
CCCCHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCEEEEECHHHHHHHHCC
GEADWVKVIDQFYKPFEKEVAHAETGIAKIQIKDEPAGFNCDICGAPMVIKMGRYGKFYA
CCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEE
CSRFPNCRNTKAIVKEIGVVCPKCHIGQVVERKSKRNRVFYGCSRYPDCDFVSWDKPVGR
ECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCC
DCPVCGHFLVEKKSKAGTQVVCPNNDYKEALQKA
CCCHHHHHHHHHHCCCCCEEECCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]