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The map label for this gene is rluB [H]

Identifier: 116494858

GI number: 116494858

Start: 1360828

End: 1361571

Strand: Direct

Name: rluB [H]

Synonym: LSEI_1372

Alternate gene names: 116494858

Gene position: 1360828-1361571 (Clockwise)

Preceding gene: 116494857

Following gene: 116494859

Centisome position: 47.0

GC content: 45.3

Gene sequence:

>744_bases
ATGACAACCAAAACTGAACGGCTTCAAAAAATTATCGCAAATGCCGGTATTGCTTCGCGTCGACATGCGGAAACGTTGAT
TGCAACTGGCCATGTCAAAGTTAATGGCCAAATTGAAACTGAAATGGGCATCAAGATTGGTCCGCATGATGAAGTCGAAG
TGGATGGCGTGCCGATTGGCAAAGAGGCTAAGCGCTACTTTCTCTTTTATAAACCGCGCGGCGTGATTAGTGCTGTTCAA
GATAATAAGGGACGTGATGTCGTCACCGATTACTTTAAGGATATTACTGAGCGTCTTTATCCAGTTGGCCGATTAGATTA
CGATACAAGCGGCTTACTTGTGATGACAAACGATGGCGATTTAGCCAACCATTTGATGCATCCACGTTACGCGCTTGAGA
AAAAATATGTAGCCAAGGTTCAAGGTGTACCGAATCGACCTGCCTTGGCTCCGCTCAAAACAGGTGTCGAGGTTGATGGC
AAGATGACTGCGCCTGCTAAAGTAGACATTATCTCGACTGATAAGGCTAAAAAAACGGCGATCGTATCTTTGACCATTCA
TCAAGGCATGAATCATCAGGTTAAAAAGATGTTTAAAGCAGTCGGCTTCCCAGTTCTAAAACTATCTCGGGAAGCGTATG
GCAACTTAACTTTGGTGGGGTTACAGCCAGGTGAACATCGGCCAATCAAGCCGCAAGAACTGCAAGCCTTGCGAAAACTA
GCGGGTGAATCGGAAAATAAGTAG

Upstream 100 bases:

>100_bases
GCCGACACAGGTGAGGTTGATTTGTTTACGGCATTTCGGCACGATGATGATCAAGAGGAAGCTACTATAGACGAAAAGAA
AATGGAGCAAAATCAACAAC

Downstream 100 bases:

>100_bases
TTGGTGAAATTGTTAACGCTTTCAGTGCAAACGCTTGAACTTTTCCGGTGCCTTTGCTAAAGTAAAGCTGTTAATTAAAT
AATCTTGGTTCGTCTTCAGG

Product: 16S rRNA uridine-516 pseudouridylate synthase related enzyme

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MTTKTERLQKIIANAGIASRRHAETLIATGHVKVNGQIETEMGIKIGPHDEVEVDGVPIGKEAKRYFLFYKPRGVISAVQ
DNKGRDVVTDYFKDITERLYPVGRLDYDTSGLLVMTNDGDLANHLMHPRYALEKKYVAKVQGVPNRPALAPLKTGVEVDG
KMTAPAKVDIISTDKAKKTAIVSLTIHQGMNHQVKKMFKAVGFPVLKLSREAYGNLTLVGLQPGEHRPIKPQELQALRKL
AGESENK

Sequences:

>Translated_247_residues
MTTKTERLQKIIANAGIASRRHAETLIATGHVKVNGQIETEMGIKIGPHDEVEVDGVPIGKEAKRYFLFYKPRGVISAVQ
DNKGRDVVTDYFKDITERLYPVGRLDYDTSGLLVMTNDGDLANHLMHPRYALEKKYVAKVQGVPNRPALAPLKTGVEVDG
KMTAPAKVDIISTDKAKKTAIVSLTIHQGMNHQVKKMFKAVGFPVLKLSREAYGNLTLVGLQPGEHRPIKPQELQALRKL
AGESENK
>Mature_246_residues
TTKTERLQKIIANAGIASRRHAETLIATGHVKVNGQIETEMGIKIGPHDEVEVDGVPIGKEAKRYFLFYKPRGVISAVQD
NKGRDVVTDYFKDITERLYPVGRLDYDTSGLLVMTNDGDLANHLMHPRYALEKKYVAKVQGVPNRPALAPLKTGVEVDGK
MTAPAKVDIISTDKAKKTAIVSLTIHQGMNHQVKKMFKAVGFPVLKLSREAYGNLTLVGLQPGEHRPIKPQELQALRKLA
GESENK

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=237, Percent_Identity=34.5991561181435, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1787524, Length=223, Percent_Identity=34.0807174887892, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1790453, Length=244, Percent_Identity=34.4262295081967, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI87081838, Length=181, Percent_Identity=33.7016574585635, Blast_Score=102, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 27287; Mature: 27156

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKTERLQKIIANAGIASRRHAETLIATGHVKVNGQIETEMGIKIGPHDEVEVDGVPIG
CCCHHHHHHHHHHHCCCCCHHHHHEEEEECEEEECCEEEEECCCEECCCCCEEECCCCCC
KEAKRYFLFYKPRGVISAVQDNKGRDVVTDYFKDITERLYPVGRLDYDTSGLLVMTNDGD
CCCCEEEEEECCCCCEEHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCH
LANHLMHPRYALEKKYVAKVQGVPNRPALAPLKTGVEVDGKMTAPAKVDIISTDKAKKTA
HHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHCCCCEECCEECCCEEEEEEECCCCCCEE
IVSLTIHQGMNHQVKKMFKAVGFPVLKLSREAYGNLTLVGLQPGEHRPIKPQELQALRKL
EEEEEECCCCCHHHHHHHHHHCCHHEEECHHHCCCEEEEEECCCCCCCCCHHHHHHHHHH
AGESENK
HCCCCCC
>Mature Secondary Structure 
TTKTERLQKIIANAGIASRRHAETLIATGHVKVNGQIETEMGIKIGPHDEVEVDGVPIG
CCHHHHHHHHHHHCCCCCHHHHHEEEEECEEEECCEEEEECCCEECCCCCEEECCCCCC
KEAKRYFLFYKPRGVISAVQDNKGRDVVTDYFKDITERLYPVGRLDYDTSGLLVMTNDGD
CCCCEEEEEECCCCCEEHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCH
LANHLMHPRYALEKKYVAKVQGVPNRPALAPLKTGVEVDGKMTAPAKVDIISTDKAKKTA
HHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHCCCCEECCEECCCEEEEEEECCCCCCEE
IVSLTIHQGMNHQVKKMFKAVGFPVLKLSREAYGNLTLVGLQPGEHRPIKPQELQALRKL
EEEEEECCCCCHHHHHHHHHHCCHHEEECHHHCCCEEEEEECCCCCCCCCHHHHHHHHHH
AGESENK
HCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]