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The map label for this gene is pyk [H]

Identifier: 116494851

GI number: 116494851

Start: 1354628

End: 1356394

Strand: Direct

Name: pyk [H]

Synonym: LSEI_1365

Alternate gene names: 116494851

Gene position: 1354628-1356394 (Clockwise)

Preceding gene: 116494850

Following gene: 116494852

Centisome position: 46.79

GC content: 47.88

Gene sequence:

>1767_bases
ATGAAAAAAACCAAGATCGTCAGCACGCTTGGACCTGCAAGTAACACCACTGATATCATCGTTAAGTTGATTGAAGCGGG
TGCCAACGTTTTCCGTTTCAATTTCTCACATGGTGATCATGAAGAGCATTTGGCACGGATGAACATGGTTCACGAAGCTG
AAAAGATCACGGGTAAGACCGTTGGTATCATGCTGGATACGAAGGGTGCCGAAATTCGGACAACTCTTGAAGATACCCCG
AATGGTAAGATCGATTTTCACACCGGTGATAAAGTTCGCATCTCCATGGATGCAAGTCTGAAAGGCACCAAGGAAAAAGT
TGCTGTTACCTATCCTGGCCTTTATGATGACACTCACGTTGGCGGCCATGTTTTGTTTGATGATGGCTTGATTGACATGA
AGATCACCGAAAAGGACGAAAAGAACCGTGAACTCGTTACCGTTGTTCAAAACGACGGTGTCCTTGGTGGTAAGAAAGGT
GTCAACGCTCCTGGTGTTGCCATCAACTTGCCTGGGATTACGGAAAAAGACTCCGATGACATTCGTTTCGGCTTGGACAA
CGGGATCAACTTCATTGCTGCTTCCTTCGTTCGTAAGCCTCAAGACGTTTTAGACATTCGTGAACTCCTTGAAGAAAAGA
ACGCACTTAATGTTCAGATCTTCCCTAAGATCGAATCACAAGAAGGCATCGACAACATTGATGACATCTTGAAGGTTTCC
GATGGCTTAATGGTTGCTCGTGGCGACATGGGTGTTGAAATTCCATTTGAACATGTTCCGATCGTTCAGAAGCGCTTGAT
CAAGAAGTGCAACTCTTTGGGTAAGCCGGTTATCACGGCTACACAAATGTTGGATTCCATGCAAGAAAACCCACGTCCTA
CCCGTGCCGAAGTAAACGACGTTGCCAATGCCGTGTTTGATGGCACCGATGCAACGATGCTTTCTGGCGAATCTGCTAAT
GGTGACTATCCGGTTGAATCCGTTGCTGCAATGGCTCGGATTGACGAATACACCGAAGCAGCGATGATGGATCAGGATGC
CTTCGCATTGAAGGAATCCAGTAACAAGAACGTCACCGAAGCTGTTGGCCAAAGTGTTGCACACACGGCTCGCAACTTAG
GGGTTAAGACCATTGTTGCTGCGACCGAATCCGGCTACACTGCCCGCATGATTTCCAAGTATCGTCCGAAGGCTGACATT
CTTGCCATTACCTTCAGCGAAAAGACTCAACGCGGCCTGATGGTCAACTGGGGCGTTTACCCAATCATTGCTGAAAAGCC
TGCTAACACCGATGCTATGTTCGACTTGGCAACCAAGAAGGCCCAAGACCTTGGCTTTGCTAAGGAAGGCGATCTGATCT
TGATCACTGCCGGTGTTCCTGTTGGTGAATCTGGCACAACCAACGTAATGAAGGTTCAACTCATTGGCTCCAAGCTGGTT
CAAGGCTCAGGTGTCGGCGACGAATCCACGATTGGTAAGGCAGTCATCGCAAGCAACGCTCAAGAAGCTGCTGCTAAGAT
GCAAAAGGGTGACATTCTGGTTGTTAAGACGACTGACAAAGATTATTTGCCAGCAATCGAAAAAGCAGCTGCCTTGGTTG
TTGAAACCGGTGGCTTGACCAGTCACGCAGCTGTTGTTGGTATTGCTATGGGTATCCCAGTTGTTGTTGGTGCTGAAAAT
GCTACCAGCGTCATCTCCGATGGCCAGATCATTACTGTTGACTCCCGTCGTGGGATCATCTACAAAGGTGCTACCAACGC
CCTTTAA

Upstream 100 bases:

>100_bases
GAACTTTCGCTCTACACCTTAGCGGAAGAGTTAACATTTTAATCTCGTAAACAAACGACATTAACGTCTGTTTAAATAAT
TTCAAGGAGCGATTTCACTT

Downstream 100 bases:

>100_bases
TCTTCAAAATGACCTGTTAAAGGTTAGGAAAAACTCTCAAGGAAACTTGGGAGTTTTTTTCTTCCAAAAAAACAGTAGCG
TTGCCTGTTTTGAGCAACTT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 588; Mature: 588

Protein sequence:

>588_residues
MKKTKIVSTLGPASNTTDIIVKLIEAGANVFRFNFSHGDHEEHLARMNMVHEAEKITGKTVGIMLDTKGAEIRTTLEDTP
NGKIDFHTGDKVRISMDASLKGTKEKVAVTYPGLYDDTHVGGHVLFDDGLIDMKITEKDEKNRELVTVVQNDGVLGGKKG
VNAPGVAINLPGITEKDSDDIRFGLDNGINFIAASFVRKPQDVLDIRELLEEKNALNVQIFPKIESQEGIDNIDDILKVS
DGLMVARGDMGVEIPFEHVPIVQKRLIKKCNSLGKPVITATQMLDSMQENPRPTRAEVNDVANAVFDGTDATMLSGESAN
GDYPVESVAAMARIDEYTEAAMMDQDAFALKESSNKNVTEAVGQSVAHTARNLGVKTIVAATESGYTARMISKYRPKADI
LAITFSEKTQRGLMVNWGVYPIIAEKPANTDAMFDLATKKAQDLGFAKEGDLILITAGVPVGESGTTNVMKVQLIGSKLV
QGSGVGDESTIGKAVIASNAQEAAAKMQKGDILVVKTTDKDYLPAIEKAAALVVETGGLTSHAAVVGIAMGIPVVVGAEN
ATSVISDGQIITVDSRRGIIYKGATNAL

Sequences:

>Translated_588_residues
MKKTKIVSTLGPASNTTDIIVKLIEAGANVFRFNFSHGDHEEHLARMNMVHEAEKITGKTVGIMLDTKGAEIRTTLEDTP
NGKIDFHTGDKVRISMDASLKGTKEKVAVTYPGLYDDTHVGGHVLFDDGLIDMKITEKDEKNRELVTVVQNDGVLGGKKG
VNAPGVAINLPGITEKDSDDIRFGLDNGINFIAASFVRKPQDVLDIRELLEEKNALNVQIFPKIESQEGIDNIDDILKVS
DGLMVARGDMGVEIPFEHVPIVQKRLIKKCNSLGKPVITATQMLDSMQENPRPTRAEVNDVANAVFDGTDATMLSGESAN
GDYPVESVAAMARIDEYTEAAMMDQDAFALKESSNKNVTEAVGQSVAHTARNLGVKTIVAATESGYTARMISKYRPKADI
LAITFSEKTQRGLMVNWGVYPIIAEKPANTDAMFDLATKKAQDLGFAKEGDLILITAGVPVGESGTTNVMKVQLIGSKLV
QGSGVGDESTIGKAVIASNAQEAAAKMQKGDILVVKTTDKDYLPAIEKAAALVVETGGLTSHAAVVGIAMGIPVVVGAEN
ATSVISDGQIITVDSRRGIIYKGATNAL
>Mature_588_residues
MKKTKIVSTLGPASNTTDIIVKLIEAGANVFRFNFSHGDHEEHLARMNMVHEAEKITGKTVGIMLDTKGAEIRTTLEDTP
NGKIDFHTGDKVRISMDASLKGTKEKVAVTYPGLYDDTHVGGHVLFDDGLIDMKITEKDEKNRELVTVVQNDGVLGGKKG
VNAPGVAINLPGITEKDSDDIRFGLDNGINFIAASFVRKPQDVLDIRELLEEKNALNVQIFPKIESQEGIDNIDDILKVS
DGLMVARGDMGVEIPFEHVPIVQKRLIKKCNSLGKPVITATQMLDSMQENPRPTRAEVNDVANAVFDGTDATMLSGESAN
GDYPVESVAAMARIDEYTEAAMMDQDAFALKESSNKNVTEAVGQSVAHTARNLGVKTIVAATESGYTARMISKYRPKADI
LAITFSEKTQRGLMVNWGVYPIIAEKPANTDAMFDLATKKAQDLGFAKEGDLILITAGVPVGESGTTNVMKVQLIGSKLV
QGSGVGDESTIGKAVIASNAQEAAAKMQKGDILVVKTTDKDYLPAIEKAAALVVETGGLTSHAAVVGIAMGIPVVVGAEN
ATSVISDGQIITVDSRRGIIYKGATNAL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI10835121, Length=489, Percent_Identity=40.2862985685072, Blast_Score=364, Evalue=1e-100,
Organism=Homo sapiens, GI32967597, Length=489, Percent_Identity=40.2862985685072, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI33286422, Length=489, Percent_Identity=39.6728016359918, Blast_Score=354, Evalue=1e-97,
Organism=Homo sapiens, GI33286420, Length=489, Percent_Identity=39.6728016359918, Blast_Score=354, Evalue=1e-97,
Organism=Homo sapiens, GI33286418, Length=489, Percent_Identity=39.6728016359918, Blast_Score=347, Evalue=2e-95,
Organism=Homo sapiens, GI310128732, Length=290, Percent_Identity=41.3793103448276, Blast_Score=218, Evalue=1e-56,
Organism=Homo sapiens, GI310128730, Length=290, Percent_Identity=41.3793103448276, Blast_Score=218, Evalue=1e-56,
Organism=Homo sapiens, GI310128736, Length=238, Percent_Identity=37.8151260504202, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI310128734, Length=238, Percent_Identity=37.8151260504202, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI310128738, Length=217, Percent_Identity=38.2488479262673, Blast_Score=142, Evalue=6e-34,
Organism=Escherichia coli, GI1787965, Length=475, Percent_Identity=47.3684210526316, Blast_Score=416, Evalue=1e-117,
Organism=Escherichia coli, GI1788160, Length=479, Percent_Identity=38.8308977035491, Blast_Score=282, Evalue=4e-77,
Organism=Escherichia coli, GI1787994, Length=144, Percent_Identity=30.5555555555556, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17544584, Length=485, Percent_Identity=38.7628865979381, Blast_Score=341, Evalue=5e-94,
Organism=Caenorhabditis elegans, GI71984413, Length=486, Percent_Identity=39.5061728395062, Blast_Score=320, Evalue=1e-87,
Organism=Caenorhabditis elegans, GI17506829, Length=486, Percent_Identity=39.5061728395062, Blast_Score=320, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI71984406, Length=486, Percent_Identity=39.5061728395062, Blast_Score=320, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI17506831, Length=486, Percent_Identity=39.5061728395062, Blast_Score=319, Evalue=2e-87,
Organism=Saccharomyces cerevisiae, GI6319279, Length=492, Percent_Identity=39.0243902439024, Blast_Score=328, Evalue=2e-90,
Organism=Saccharomyces cerevisiae, GI6324923, Length=497, Percent_Identity=39.0342052313883, Blast_Score=312, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24648964, Length=484, Percent_Identity=40.0826446280992, Blast_Score=341, Evalue=1e-93,
Organism=Drosophila melanogaster, GI28571814, Length=486, Percent_Identity=40.3292181069959, Blast_Score=340, Evalue=2e-93,
Organism=Drosophila melanogaster, GI24648966, Length=414, Percent_Identity=42.2705314009662, Blast_Score=310, Evalue=2e-84,
Organism=Drosophila melanogaster, GI24581235, Length=484, Percent_Identity=35.1239669421488, Blast_Score=284, Evalue=2e-76,
Organism=Drosophila melanogaster, GI24646914, Length=268, Percent_Identity=41.044776119403, Blast_Score=201, Evalue=2e-51,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 62807; Mature: 62807

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTKIVSTLGPASNTTDIIVKLIEAGANVFRFNFSHGDHEEHLARMNMVHEAEKITGKT
CCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCE
VGIMLDTKGAEIRTTLEDTPNGKIDFHTGDKVRISMDASLKGTKEKVAVTYPGLYDDTHV
EEEEEECCCCEEEEEECCCCCCEEEEECCCEEEEEECCCCCCCCCEEEEEECCCCCCCCC
GGHVLFDDGLIDMKITEKDEKNRELVTVVQNDGVLGGKKGVNAPGVAINLPGITEKDSDD
CCEEEEECCEEEEEECCCCCCCCEEEEEEECCCEECCCCCCCCCCEEEECCCCCCCCCCC
IRFGLDNGINFIAASFVRKPQDVLDIRELLEEKNALNVQIFPKIESQEGIDNIDDILKVS
EEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHC
DGLMVARGDMGVEIPFEHVPIVQKRLIKKCNSLGKPVITATQMLDSMQENPRPTRAEVND
CCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHH
VANAVFDGTDATMLSGESANGDYPVESVAAMARIDEYTEAAMMDQDAFALKESSNKNVTE
HHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHH
AVGQSVAHTARNLGVKTIVAATESGYTARMISKYRPKADILAITFSEKTQRGLMVNWGVY
HHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHCCCCCEEEEEEECCCCCCCEEEECCEE
PIIAEKPANTDAMFDLATKKAQDLGFAKEGDLILITAGVPVGESGTTNVMKVQLIGSKLV
EEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEECHHHH
QGSGVGDESTIGKAVIASNAQEAAAKMQKGDILVVKTTDKDYLPAIEKAAALVVETGGLT
CCCCCCCCHHHCHHHHHCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHCEEEEEECCCCC
SHAAVVGIAMGIPVVVGAENATSVISDGQIITVDSRRGIIYKGATNAL
HHHHHHHHHHCCCEEEECCCCHHHCCCCEEEEEECCCCEEEECCCCCC
>Mature Secondary Structure
MKKTKIVSTLGPASNTTDIIVKLIEAGANVFRFNFSHGDHEEHLARMNMVHEAEKITGKT
CCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCE
VGIMLDTKGAEIRTTLEDTPNGKIDFHTGDKVRISMDASLKGTKEKVAVTYPGLYDDTHV
EEEEEECCCCEEEEEECCCCCCEEEEECCCEEEEEECCCCCCCCCEEEEEECCCCCCCCC
GGHVLFDDGLIDMKITEKDEKNRELVTVVQNDGVLGGKKGVNAPGVAINLPGITEKDSDD
CCEEEEECCEEEEEECCCCCCCCEEEEEEECCCEECCCCCCCCCCEEEECCCCCCCCCCC
IRFGLDNGINFIAASFVRKPQDVLDIRELLEEKNALNVQIFPKIESQEGIDNIDDILKVS
EEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHC
DGLMVARGDMGVEIPFEHVPIVQKRLIKKCNSLGKPVITATQMLDSMQENPRPTRAEVND
CCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHH
VANAVFDGTDATMLSGESANGDYPVESVAAMARIDEYTEAAMMDQDAFALKESSNKNVTE
HHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHH
AVGQSVAHTARNLGVKTIVAATESGYTARMISKYRPKADILAITFSEKTQRGLMVNWGVY
HHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHCCCCCEEEEEEECCCCCCCEEEECCEE
PIIAEKPANTDAMFDLATKKAQDLGFAKEGDLILITAGVPVGESGTTNVMKVQLIGSKLV
EEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEECHHHH
QGSGVGDESTIGKAVIASNAQEAAAKMQKGDILVVKTTDKDYLPAIEKAAALVVETGGLT
CCCCCCCCHHHCHHHHHCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHCEEEEEECCCCC
SHAAVVGIAMGIPVVVGAENATSVISDGQIITVDSRRGIIYKGATNAL
HHHHHHHHHHCCCEEEECCCCHHHCCCCEEEEEECCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8755908; 8366023; 8274531 [H]