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The map label for this gene is fgs [H]

Identifier: 116494744

GI number: 116494744

Start: 1231867

End: 1233144

Strand: Direct

Name: fgs [H]

Synonym: LSEI_1252

Alternate gene names: 116494744

Gene position: 1231867-1233144 (Clockwise)

Preceding gene: 116494743

Following gene: 116494745

Centisome position: 42.55

GC content: 50.78

Gene sequence:

>1278_bases
ATGGACTACACCGAAAGTGTCGCGTATATTCATTCGTTTCCGCGCTTGGCCAAAACTGGCGATCATCAGCGCATTCTCAC
CTTGCTGCATGCCTTGGGTGACCCGCAAAAACGAGGCCATTATATTCATGTCACTGGGACAAATGGTAAAGGCTCAGCCG
CCAATGCCATTGCCCATATCCTTGAAGCAAGCGGTTTGAGTGTCGGTTTGTATACATCACCGTTCATTATGCGGTTTAAT
GAGCGCATGATGATTGATCATCAGCCCATTCCTGATGCTGAGCTTGTTAAAGCGGTCGCTGTCGGACAACAGGCCTTAAC
CCAGCTGCGGCAAGCGACACCAGATTTCGGGGTCACGGAATTCGAATTTATCACGGCTCTGGCCTTTTGGTATTTTGATC
AAAAACAGGTGGATTTGGCAGTGATTGAGGTGGGGATTGGTGGCGACACGGATTCCACCAATGTGATCACGCCGGTTGTA
AGTGTGCTGACTGAGGTAGCTTTAGATCATCAAAAACTGCTTGGCGATACGATTACTGAGATTGCCACGCATAAAGCTGG
CATCATTAAACCGCACGTCCCGGTTGTGACTGGCAATTTGGTACCAGATGCTGATGCTGTTGTGGCGGCAAAGGTTGCAG
CAACAGCTAGCACGTGGTTGCGATTTGACCGCGACTTTTCCGTTCCCAAAGCGCGCTTGCATGGCTGGGGTCAGCGATTG
ACTTATGCCGACAAGGATGGTGAGCTGACAGACATAGATATACCGCTGGTTGGTGATTACCAACAGCGAAATATGGCAAT
TGCGATTCAGACGGCAAAAGTCTATGCCTCTGCAACGAATTGGCCGTTGAAGTCGCAGGAAATTCGCCAAGGGCTAGCGG
CCAGTCGATGGCCGGCGCGATTAGAGAAGTTAAGTGACGAACCACTGATTGTGATTGACGGCGCCCATAATCCGGACGGC
ATCAAGGGATTAATTGCCGCATTGCACCAGCTCTTTAAGCAACCGGTGACAGTGATTGCTGGCATTTTGGCTGACAAGGA
TTATCACACCATGGCCGATGCACTAACGAAGGCATTTCATACCGTGTATCTCGTACCGGTACCAGGCAATGCCCGGGCTT
TGCCTGAGGCTGGGTATGCAGCCTTGCATGAAGGCCAATTAAAGGATTCCTGGCAAGAAGCAATGGCCGCGAGCCTTGTT
GACGATCCGGATCAGCCAATCATCTTAACCGGCTCGCTTTATCTGGCCGCTGATGTTCGACAAACTTTAAGAGCGTGA

Upstream 100 bases:

>100_bases
ACTTCTCCAGAATTTCTGGTATCATGAAACTTATGAAGCTAGCACTTGCACGCTGGCATAGAAACCGGGCGCGTTCATGC
CCTGAGGAAAGAAGTTAACC

Downstream 100 bases:

>100_bases
GCCAACCCGGGTAGAAACCGGAGTGTAAGCGGCCTTGAAGTCTAAATGGCTGATCCTTGGCCATTTAGACTTCAAGGTCC
TTATGTGCAGGTTTCTGCGC

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MDYTESVAYIHSFPRLAKTGDHQRILTLLHALGDPQKRGHYIHVTGTNGKGSAANAIAHILEASGLSVGLYTSPFIMRFN
ERMMIDHQPIPDAELVKAVAVGQQALTQLRQATPDFGVTEFEFITALAFWYFDQKQVDLAVIEVGIGGDTDSTNVITPVV
SVLTEVALDHQKLLGDTITEIATHKAGIIKPHVPVVTGNLVPDADAVVAAKVAATASTWLRFDRDFSVPKARLHGWGQRL
TYADKDGELTDIDIPLVGDYQQRNMAIAIQTAKVYASATNWPLKSQEIRQGLAASRWPARLEKLSDEPLIVIDGAHNPDG
IKGLIAALHQLFKQPVTVIAGILADKDYHTMADALTKAFHTVYLVPVPGNARALPEAGYAALHEGQLKDSWQEAMAASLV
DDPDQPIILTGSLYLAADVRQTLRA

Sequences:

>Translated_425_residues
MDYTESVAYIHSFPRLAKTGDHQRILTLLHALGDPQKRGHYIHVTGTNGKGSAANAIAHILEASGLSVGLYTSPFIMRFN
ERMMIDHQPIPDAELVKAVAVGQQALTQLRQATPDFGVTEFEFITALAFWYFDQKQVDLAVIEVGIGGDTDSTNVITPVV
SVLTEVALDHQKLLGDTITEIATHKAGIIKPHVPVVTGNLVPDADAVVAAKVAATASTWLRFDRDFSVPKARLHGWGQRL
TYADKDGELTDIDIPLVGDYQQRNMAIAIQTAKVYASATNWPLKSQEIRQGLAASRWPARLEKLSDEPLIVIDGAHNPDG
IKGLIAALHQLFKQPVTVIAGILADKDYHTMADALTKAFHTVYLVPVPGNARALPEAGYAALHEGQLKDSWQEAMAASLV
DDPDQPIILTGSLYLAADVRQTLRA
>Mature_425_residues
MDYTESVAYIHSFPRLAKTGDHQRILTLLHALGDPQKRGHYIHVTGTNGKGSAANAIAHILEASGLSVGLYTSPFIMRFN
ERMMIDHQPIPDAELVKAVAVGQQALTQLRQATPDFGVTEFEFITALAFWYFDQKQVDLAVIEVGIGGDTDSTNVITPVV
SVLTEVALDHQKLLGDTITEIATHKAGIIKPHVPVVTGNLVPDADAVVAAKVAATASTWLRFDRDFSVPKARLHGWGQRL
TYADKDGELTDIDIPLVGDYQQRNMAIAIQTAKVYASATNWPLKSQEIRQGLAASRWPARLEKLSDEPLIVIDGAHNPDG
IKGLIAALHQLFKQPVTVIAGILADKDYHTMADALTKAFHTVYLVPVPGNARALPEAGYAALHEGQLKDSWQEAMAASLV
DDPDQPIILTGSLYLAADVRQTLRA

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=320, Percent_Identity=31.25, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI66932984, Length=320, Percent_Identity=31.25, Blast_Score=133, Evalue=3e-31,
Organism=Escherichia coli, GI1788654, Length=390, Percent_Identity=31.025641025641, Blast_Score=159, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17553148, Length=354, Percent_Identity=27.4011299435028, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI71984923, Length=354, Percent_Identity=27.4011299435028, Blast_Score=131, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17553150, Length=354, Percent_Identity=27.4011299435028, Blast_Score=131, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6323760, Length=425, Percent_Identity=28.9411764705882, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6324815, Length=339, Percent_Identity=27.7286135693215, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24641571, Length=286, Percent_Identity=30.4195804195804, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24581568, Length=171, Percent_Identity=29.2397660818713, Blast_Score=85, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 46177; Mature: 46177

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00687 ALDEHYDE_DEHYDR_GLU ; PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYTESVAYIHSFPRLAKTGDHQRILTLLHALGDPQKRGHYIHVTGTNGKGSAANAIAHI
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHCCCEEEEECCCCCCHHHHHHHHH
LEASGLSVGLYTSPFIMRFNERMMIDHQPIPDAELVKAVAVGQQALTQLRQATPDFGVTE
HHHCCCEEEEECCCCCEEECCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHH
FEFITALAFWYFDQKQVDLAVIEVGIGGDTDSTNVITPVVSVLTEVALDHQKLLGDTITE
HHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IATHKAGIIKPHVPVVTGNLVPDADAVVAAKVAATASTWLRFDRDFSVPKARLHGWGQRL
HHHCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCC
TYADKDGELTDIDIPLVGDYQQRNMAIAIQTAKVYASATNWPLKSQEIRQGLAASRWPAR
EEECCCCCEEEEECCEECCCCCCCEEEEEEEEEEEEECCCCCCCHHHHHHHHHHCCCHHH
LEKLSDEPLIVIDGAHNPDGIKGLIAALHQLFKQPVTVIAGILADKDYHTMADALTKAFH
HHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
TVYLVPVPGNARALPEAGYAALHEGQLKDSWQEAMAASLVDDPDQPIILTGSLYLAADVR
EEEEEECCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEECCEEEHHHHH
QTLRA
HHHCC
>Mature Secondary Structure
MDYTESVAYIHSFPRLAKTGDHQRILTLLHALGDPQKRGHYIHVTGTNGKGSAANAIAHI
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHCCCEEEEECCCCCCHHHHHHHHH
LEASGLSVGLYTSPFIMRFNERMMIDHQPIPDAELVKAVAVGQQALTQLRQATPDFGVTE
HHHCCCEEEEECCCCCEEECCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHH
FEFITALAFWYFDQKQVDLAVIEVGIGGDTDSTNVITPVVSVLTEVALDHQKLLGDTITE
HHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IATHKAGIIKPHVPVVTGNLVPDADAVVAAKVAATASTWLRFDRDFSVPKARLHGWGQRL
HHHCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCC
TYADKDGELTDIDIPLVGDYQQRNMAIAIQTAKVYASATNWPLKSQEIRQGLAASRWPAR
EEECCCCCEEEEECCEECCCCCCCEEEEEEEEEEEEECCCCCCCHHHHHHHHHHCCCHHH
LEKLSDEPLIVIDGAHNPDGIKGLIAALHQLFKQPVTVIAGILADKDYHTMADALTKAFH
HHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
TVYLVPVPGNARALPEAGYAALHEGQLKDSWQEAMAASLVDDPDQPIILTGSLYLAADVR
EEEEEECCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEECCEEEHHHHH
QTLRA
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2105929; 9618466 [H]