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The map label for this gene is glyA

Identifier: 116494654

GI number: 116494654

Start: 1143033

End: 1144265

Strand: Direct

Name: glyA

Synonym: LSEI_1158

Alternate gene names: 116494654

Gene position: 1143033-1144265 (Clockwise)

Preceding gene: 116494653

Following gene: 116494655

Centisome position: 39.48

GC content: 48.01

Gene sequence:

>1233_bases
ATGGATTTCATGGCACAAGATCCAGAAGTTTTCGGTGCAATTCATAACGAAGAGGAACGACAAGAACATAATATTGAACT
TATCGCTTCAGAAAACATTGTTTCCCCAGCGGTTCGGGCGGCACAAGGGTCAGTGCTGACGAATAAGTATTCTGAAGGTT
ATCCTGGCCATCGTTATTATGGTGGTAATCAATACATTGATGTGGTTGAGAATCTCGCCATTGATCGCGCTAAAAAATTG
TTTGGGGCCGAATTTGCAAATGTACAGCCGCATTCCGGTTCACAGGCAAATATGGCCACGTATCGCGCCTTTTTAGAAGA
TGGCGATAAAGTGCTGGCAATGGATCTAACCGATGGCGGCCATCTGACTCACGGCAGCCCAGTTAGTTTTAGTGGTCAGG
AATATCATTTCTACCATTATGGTTTGGATCCGAAGACAGAACGGCTAAATTACGCCAAGATTCGTGAGCAGGCTGAACAG
GTGCAACCGCGAATGATTGTGGCGGGGGCTTCGGCTTATAGCCGCGAGATTGATTTCAAAAAATTCCGCGAAATTGCCGA
TCATGTTGGGGCCTTTTTGATGGTCGACATGGCGCATATTGCCGGGTTGGTTGCCGCAGGTTTGCATATGAATCCGGTGC
CTTACGCTGATGTGGTCACAACAACGACGCATAAAACGCTGCGCGGCCCCCGTGGCGGCTTGATCCTTGCTAAGGCGCAA
TACGGCAAGGCCATTAATTCAGCACTTTTCCCCGGTATTCAAGGCGGGCCGCTTGATCACGTGGTTGCAGCAAAAGCAGT
TGCATTAGGCGAAGCGTTGCAGCCATCTTTTAAAACATATGCTCAGCATATTCTGGATAACATGCAGGCGATGGTGAGTG
GTTTTGAAGAAGATCCGCATTTGCGGTTGATTTCTGGTGGTTCTGATAACCACATGGTCTTGATTGATGTAACGGGTTAT
GGCGTAAATGGTCGCCAAGTTCAAGATTTACTGGATGAAGTTGGTATCACGACTAACAAAAATCAAATTCCAGGAGAGCA
GAATGGCCCGTTTAAAACCAGCGGTATCCGAGTAGGTACGGCTGCCATTACCACTCGCGGCTTTACAGCAGATGAGAGCA
AACGAGTTGGCGAATTGATCAGTGCCGCGATTGCCCAGCGTGATGATCAGCCAGCTTTGGACCAGATTCATCAAGAAGTG
CTTGCACTGACAGCCCGTCATCCTTTATCATAG

Upstream 100 bases:

>100_bases
GCTATAATGAGACCAACTGACAGAGCCTTTGTGAGTTGGTTTTTTAATAAAAGCAAATGCAACGGGTGTTTTGATGGCGC
ATTTTAAAGGAGGAACTTGC

Downstream 100 bases:

>100_bases
TTAGGGTTTGCAAACAAATGATTATCAAAGAAAACGTGATTGACTTCTTTTGAAACCGGTCTATCAGAGGGCTTAGGACG
TGATGCTCACGGTAGCACAT

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MDFMAQDPEVFGAIHNEEERQEHNIELIASENIVSPAVRAAQGSVLTNKYSEGYPGHRYYGGNQYIDVVENLAIDRAKKL
FGAEFANVQPHSGSQANMATYRAFLEDGDKVLAMDLTDGGHLTHGSPVSFSGQEYHFYHYGLDPKTERLNYAKIREQAEQ
VQPRMIVAGASAYSREIDFKKFREIADHVGAFLMVDMAHIAGLVAAGLHMNPVPYADVVTTTTHKTLRGPRGGLILAKAQ
YGKAINSALFPGIQGGPLDHVVAAKAVALGEALQPSFKTYAQHILDNMQAMVSGFEEDPHLRLISGGSDNHMVLIDVTGY
GVNGRQVQDLLDEVGITTNKNQIPGEQNGPFKTSGIRVGTAAITTRGFTADESKRVGELISAAIAQRDDQPALDQIHQEV
LALTARHPLS

Sequences:

>Translated_410_residues
MDFMAQDPEVFGAIHNEEERQEHNIELIASENIVSPAVRAAQGSVLTNKYSEGYPGHRYYGGNQYIDVVENLAIDRAKKL
FGAEFANVQPHSGSQANMATYRAFLEDGDKVLAMDLTDGGHLTHGSPVSFSGQEYHFYHYGLDPKTERLNYAKIREQAEQ
VQPRMIVAGASAYSREIDFKKFREIADHVGAFLMVDMAHIAGLVAAGLHMNPVPYADVVTTTTHKTLRGPRGGLILAKAQ
YGKAINSALFPGIQGGPLDHVVAAKAVALGEALQPSFKTYAQHILDNMQAMVSGFEEDPHLRLISGGSDNHMVLIDVTGY
GVNGRQVQDLLDEVGITTNKNQIPGEQNGPFKTSGIRVGTAAITTRGFTADESKRVGELISAAIAQRDDQPALDQIHQEV
LALTARHPLS
>Mature_410_residues
MDFMAQDPEVFGAIHNEEERQEHNIELIASENIVSPAVRAAQGSVLTNKYSEGYPGHRYYGGNQYIDVVENLAIDRAKKL
FGAEFANVQPHSGSQANMATYRAFLEDGDKVLAMDLTDGGHLTHGSPVSFSGQEYHFYHYGLDPKTERLNYAKIREQAEQ
VQPRMIVAGASAYSREIDFKKFREIADHVGAFLMVDMAHIAGLVAAGLHMNPVPYADVVTTTTHKTLRGPRGGLILAKAQ
YGKAINSALFPGIQGGPLDHVVAAKAVALGEALQPSFKTYAQHILDNMQAMVSGFEEDPHLRLISGGSDNHMVLIDVTGY
GVNGRQVQDLLDEVGITTNKNQIPGEQNGPFKTSGIRVGTAAITTRGFTADESKRVGELISAAIAQRDDQPALDQIHQEV
LALTARHPLS

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI261862352, Length=402, Percent_Identity=43.2835820895522, Blast_Score=328, Evalue=5e-90,
Organism=Homo sapiens, GI261862350, Length=402, Percent_Identity=43.2835820895522, Blast_Score=328, Evalue=5e-90,
Organism=Homo sapiens, GI261862348, Length=402, Percent_Identity=43.2835820895522, Blast_Score=328, Evalue=5e-90,
Organism=Homo sapiens, GI19923315, Length=402, Percent_Identity=43.2835820895522, Blast_Score=328, Evalue=5e-90,
Organism=Homo sapiens, GI261862346, Length=399, Percent_Identity=43.3583959899749, Blast_Score=313, Evalue=1e-85,
Organism=Homo sapiens, GI22547186, Length=402, Percent_Identity=42.2885572139304, Blast_Score=302, Evalue=4e-82,
Organism=Homo sapiens, GI22547189, Length=387, Percent_Identity=40.5684754521964, Blast_Score=271, Evalue=6e-73,
Organism=Escherichia coli, GI1788902, Length=412, Percent_Identity=55.3398058252427, Blast_Score=436, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI25144732, Length=397, Percent_Identity=44.8362720403023, Blast_Score=338, Evalue=2e-93,
Organism=Caenorhabditis elegans, GI25144729, Length=401, Percent_Identity=44.3890274314214, Blast_Score=338, Evalue=4e-93,
Organism=Saccharomyces cerevisiae, GI6319739, Length=412, Percent_Identity=43.6893203883495, Blast_Score=345, Evalue=9e-96,
Organism=Saccharomyces cerevisiae, GI6323087, Length=402, Percent_Identity=43.7810945273632, Blast_Score=324, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24640005, Length=401, Percent_Identity=45.1371571072319, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI221329721, Length=401, Percent_Identity=45.1371571072319, Blast_Score=342, Evalue=2e-94,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_LACC3 (Q03A26)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_806388.1
- ProteinModelPortal:   Q03A26
- SMR:   Q03A26
- STRING:   Q03A26
- GeneID:   4421615
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_1158
- eggNOG:   COG0112
- HOGENOM:   HBG301263
- OMA:   THINSAL
- ProtClustDB:   PRK00011
- BioCyc:   LCAS321967:LSEI_1158-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 44586; Mature: 44586

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFMAQDPEVFGAIHNEEERQEHNIELIASENIVSPAVRAAQGSVLTNKYSEGYPGHRYY
CCCCCCCHHHHHHHCCHHHHHHCCEEEEECCCCHHHHHHHHCCCCEECCCCCCCCCCEEC
GGNQYIDVVENLAIDRAKKLFGAEFANVQPHSGSQANMATYRAFLEDGDKVLAMDLTDGG
CCCHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCC
HLTHGSPVSFSGQEYHFYHYGLDPKTERLNYAKIREQAEQVQPRMIVAGASAYSREIDFK
CCCCCCCCCCCCCEEEEEEECCCCCHHHCHHHHHHHHHHHCCCEEEEECCCHHHHCCCHH
KFREIADHVGAFLMVDMAHIAGLVAAGLHMNPVPYADVVTTTTHKTLRGPRGGLILAKAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEECCCHHHHCCCCCCEEEEEHH
YGKAINSALFPGIQGGPLDHVVAAKAVALGEALQPSFKTYAQHILDNMQAMVSGFEEDPH
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCC
LRLISGGSDNHMVLIDVTGYGVNGRQVQDLLDEVGITTNKNQIPGEQNGPFKTSGIRVGT
EEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCEEEEE
AAITTRGFTADESKRVGELISAAIAQRDDQPALDQIHQEVLALTARHPLS
HEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDFMAQDPEVFGAIHNEEERQEHNIELIASENIVSPAVRAAQGSVLTNKYSEGYPGHRYY
CCCCCCCHHHHHHHCCHHHHHHCCEEEEECCCCHHHHHHHHCCCCEECCCCCCCCCCEEC
GGNQYIDVVENLAIDRAKKLFGAEFANVQPHSGSQANMATYRAFLEDGDKVLAMDLTDGG
CCCHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCC
HLTHGSPVSFSGQEYHFYHYGLDPKTERLNYAKIREQAEQVQPRMIVAGASAYSREIDFK
CCCCCCCCCCCCCEEEEEEECCCCCHHHCHHHHHHHHHHHCCCEEEEECCCHHHHCCCHH
KFREIADHVGAFLMVDMAHIAGLVAAGLHMNPVPYADVVTTTTHKTLRGPRGGLILAKAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEECCCHHHHCCCCCCEEEEEHH
YGKAINSALFPGIQGGPLDHVVAAKAVALGEALQPSFKTYAQHILDNMQAMVSGFEEDPH
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCC
LRLISGGSDNHMVLIDVTGYGVNGRQVQDLLDEVGITTNKNQIPGEQNGPFKTSGIRVGT
EEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCEEEEE
AAITTRGFTADESKRVGELISAAIAQRDDQPALDQIHQEVLALTARHPLS
HEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA