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The map label for this gene is ywkE [H]

Identifier: 116494652

GI number: 116494652

Start: 1140835

End: 1141665

Strand: Direct

Name: ywkE [H]

Synonym: LSEI_1156

Alternate gene names: 116494652

Gene position: 1140835-1141665 (Clockwise)

Preceding gene: 116494651

Following gene: 116494653

Centisome position: 39.4

GC content: 51.02

Gene sequence:

>831_bases
ATGTCTAAAACGTATGCCGAGGCGCTCAAGTGGGCGTCTTTGTTGTTGACCAAGGACGGGATTGATCCTGACGGTGCGCG
GTATGTCTTGATGACACGTGCGGACTTCACCCCAAGTCAATTGATTTTGCATCGTCAAGATCTGATGCCTGAAACACGTT
GGCAGCAATTTCAACAGGATGTCGAACGCTTGCGTCAGTTTGAGCCGGCCCAATACATTGTCGGGGTAGCCCCTTTTTTT
GGTGAGTTGTTTAAGGTCACACCAGCAGTTTTAATTCCGCGATTTGAAACTGAGGAGCTTGTGGCTTGGGCCGCTGAAGA
GCAACGGACAGCACAAACTGGCTTGGATCTTGGTACTGGCAGCGGGGCGATTGGCTTAACCTTAGCCCGCAAGTTGCCGC
AAACAACGATGACCTTGAGCGATGTTTCACCAGAAGCACTTGCGGTCGCTAAGCAAAATGCTAAGGAACAGCAGGTGGCA
GTTCAGTTTACGGTGAGTGACTTGTTTGCAGCCCTACCAGCCCGGTATGACTTTGTTGTGACAAATTTGCCTTATATCGC
CCCAGAAGAGACGCCTGTGATGGATCAAAGCACGCTGCGTTATGAACCTAAGTTAGCATTATTTGCAGGGCATCATGGCC
TCGCCGTTTTTGAGCAGTTTGTTGCAGCGTTGCCTCAGCACTTAACAGCTGGCGGGGCGGCATACTTAGAATTCGGTTAT
CGCCAAGAGCCGGCATTGCGCGAACTTTTTGCTAAGCAACTGCCACAGGCGCAGGTGACTTTTCGACGCGATATGGCAGA
TCATCCTCGTATGGCCAAGCTGCAATTCTGA

Upstream 100 bases:

>100_bases
TTAACGCTCAATAAGTTGGATCGTATCATGAATGGCGAACTCGACGAAATTATCGATGCGTTGATCGTTCATGATCAGGC
GCAAAAGATGGAATCGCTCA

Downstream 100 bases:

>100_bases
AGGTTAAAAAGCATGGTGTGACCCTTGAACCGATCTGCTACAATTTTTCTACTAAGTTTGAGCTATTTTAAAAAGAACAT
GATTTGGAGGCAACATCTTG

Product: methylase of polypeptide chain release factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MSKTYAEALKWASLLLTKDGIDPDGARYVLMTRADFTPSQLILHRQDLMPETRWQQFQQDVERLRQFEPAQYIVGVAPFF
GELFKVTPAVLIPRFETEELVAWAAEEQRTAQTGLDLGTGSGAIGLTLARKLPQTTMTLSDVSPEALAVAKQNAKEQQVA
VQFTVSDLFAALPARYDFVVTNLPYIAPEETPVMDQSTLRYEPKLALFAGHHGLAVFEQFVAALPQHLTAGGAAYLEFGY
RQEPALRELFAKQLPQAQVTFRRDMADHPRMAKLQF

Sequences:

>Translated_276_residues
MSKTYAEALKWASLLLTKDGIDPDGARYVLMTRADFTPSQLILHRQDLMPETRWQQFQQDVERLRQFEPAQYIVGVAPFF
GELFKVTPAVLIPRFETEELVAWAAEEQRTAQTGLDLGTGSGAIGLTLARKLPQTTMTLSDVSPEALAVAKQNAKEQQVA
VQFTVSDLFAALPARYDFVVTNLPYIAPEETPVMDQSTLRYEPKLALFAGHHGLAVFEQFVAALPQHLTAGGAAYLEFGY
RQEPALRELFAKQLPQAQVTFRRDMADHPRMAKLQF
>Mature_275_residues
SKTYAEALKWASLLLTKDGIDPDGARYVLMTRADFTPSQLILHRQDLMPETRWQQFQQDVERLRQFEPAQYIVGVAPFFG
ELFKVTPAVLIPRFETEELVAWAAEEQRTAQTGLDLGTGSGAIGLTLARKLPQTTMTLSDVSPEALAVAKQNAKEQQVAV
QFTVSDLFAALPARYDFVVTNLPYIAPEETPVMDQSTLRYEPKLALFAGHHGLAVFEQFVAALPQHLTAGGAAYLEFGYR
QEPALRELFAKQLPQAQVTFRRDMADHPRMAKLQF

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=241, Percent_Identity=30.2904564315353, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1787463, Length=226, Percent_Identity=34.070796460177, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87082085, Length=184, Percent_Identity=34.2391304347826, Blast_Score=96, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24582226, Length=193, Percent_Identity=31.6062176165803, Blast_Score=92, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 30916; Mature: 30785

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTYAEALKWASLLLTKDGIDPDGARYVLMTRADFTPSQLILHRQDLMPETRWQQFQQD
CCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHH
VERLRQFEPAQYIVGVAPFFGELFKVTPAVLIPRFETEELVAWAAEEQRTAQTGLDLGTG
HHHHHHCCCHHHHEEHHHHHHHHHHHCHHHEECCCCHHHHHHHHHHHHHHHHHCCCCCCC
SGAIGLTLARKLPQTTMTLSDVSPEALAVAKQNAKEQQVAVQFTVSDLFAALPARYDFVV
CCHHHHHHHHHCCCHHEECCCCCHHHHHHHHHCCCHHHEEEEEEHHHHHHHCCCCCEEEE
TNLPYIAPEETPVMDQSTLRYEPKLALFAGHHGLAVFEQFVAALPQHLTAGGAAYLEFGY
ECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHCCC
RQEPALRELFAKQLPQAQVTFRRDMADHPRMAKLQF
CCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCEECCC
>Mature Secondary Structure 
SKTYAEALKWASLLLTKDGIDPDGARYVLMTRADFTPSQLILHRQDLMPETRWQQFQQD
CCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHH
VERLRQFEPAQYIVGVAPFFGELFKVTPAVLIPRFETEELVAWAAEEQRTAQTGLDLGTG
HHHHHHCCCHHHHEEHHHHHHHHHHHCHHHEECCCCHHHHHHHHHHHHHHHHHCCCCCCC
SGAIGLTLARKLPQTTMTLSDVSPEALAVAKQNAKEQQVAVQFTVSDLFAALPARYDFVV
CCHHHHHHHHHCCCHHEECCCCCHHHHHHHHHCCCHHHEEEEEEHHHHHHHCCCCCEEEE
TNLPYIAPEETPVMDQSTLRYEPKLALFAGHHGLAVFEQFVAALPQHLTAGGAAYLEFGY
ECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHCCC
RQEPALRELFAKQLPQAQVTFRRDMADHPRMAKLQF
CCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]