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The map label for this gene is prfA

Identifier: 116494651

GI number: 116494651

Start: 1139763

End: 1140842

Strand: Direct

Name: prfA

Synonym: LSEI_1155

Alternate gene names: 116494651

Gene position: 1139763-1140842 (Clockwise)

Preceding gene: 116494649

Following gene: 116494652

Centisome position: 39.37

GC content: 46.76

Gene sequence:

>1080_bases
ATGGATAAGATTTTTGAACAGTTGGATGGTCTGCTGGACCGTTATGAAGAACTTCAGGAGCTGATGTCAGACCCTGAAGT
GATTAGCGATACAAAGCGTTATCTTGCCTTGTCCAAAGAAGAAGGCGGCATGCGGGATGTTGTCGCCGCTTACAAGAAAT
ATAAGCAAGTGCTTTCAGATATTAAAGAAAGTGAAGAAGTTCTGCGGGAAAGCAAAGACGACGACATGGAAGCTTTGGCC
AAAGAAGACCTCGAAGACTTGCAGAAGCAAAAGGCTGACCTTGAAGATCAAATCAAGGTACTGATGCTGCCCAAGGATCC
TAACGACGATAAAAATATCATTATGGAAATTCGTGGTGCAGCAGGTGGCGACGAAGCCAGTCTTTTTGCTGGCGATTTGT
TGAATATGTATATGCATTACGCTGAGCGCCAAGGCTGGAAGACCGAGATCATTGACGCAACTCCTACTGAAGTCGGCGGG
TACAAAGAGGTTGTGGTCATGATTACTGGGGACAACGTCTACTCCAAGCTTAAGTATGAAAATGGGGCGCATCGTGTGCA
GCGCGTTCCAGTGACTGAAAGTGCTGGCCGCGTGCATACCAGCACTGCAACGGTTGGTGTGATGCCAGAGTACGATGAAG
TTGATCTAAAAATTGATCCAAAAGATATTCGGACAGATGTCTATCGGTCATCAGGCGCCGGTGGTCAGCACGTCAACAAA
ACCAGTTCGGCCGTGCGGATGACCCATATTCCGAGTGGCATTGTGGTCTCAATGCAAGATGAACGTAGTCAGCAGGAAAA
CCGGGCCCGCGCAATGCAGATTCTGCGTTCGCGTGTTTACGATTATTATGAAACCCAGAATCAGGAAAAATATGATCAGA
ATCGGAAGAATGCGATTGGGACTGGTGATCGGTCGGAACGAATCAGAACTTACAATTACCCGCAAAACCGGGTTACTGAT
CATCGAATTGGGTTAACGCTCAATAAGTTGGATCGTATCATGAATGGCGAACTCGACGAAATTATCGATGCGTTGATCGT
TCATGATCAGGCGCAAAAGATGGAATCGCTCAATGTCTAA

Upstream 100 bases:

>100_bases
TAATTTTTAAATGGCGCTTGACGATAAGTGCCCGCACTGGCGCGTTTAGCGACTAGGCGTGTCGATTGTGACGCCGGTAA
ATGCATTTTTAGGAGGAAAC

Downstream 100 bases:

>100_bases
AACGTATGCCGAGGCGCTCAAGTGGGCGTCTTTGTTGTTGACCAAGGACGGGATTGATCCTGACGGTGCGCGGTATGTCT
TGATGACACGTGCGGACTTC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MDKIFEQLDGLLDRYEELQELMSDPEVISDTKRYLALSKEEGGMRDVVAAYKKYKQVLSDIKESEEVLRESKDDDMEALA
KEDLEDLQKQKADLEDQIKVLMLPKDPNDDKNIIMEIRGAAGGDEASLFAGDLLNMYMHYAERQGWKTEIIDATPTEVGG
YKEVVVMITGDNVYSKLKYENGAHRVQRVPVTESAGRVHTSTATVGVMPEYDEVDLKIDPKDIRTDVYRSSGAGGQHVNK
TSSAVRMTHIPSGIVVSMQDERSQQENRARAMQILRSRVYDYYETQNQEKYDQNRKNAIGTGDRSERIRTYNYPQNRVTD
HRIGLTLNKLDRIMNGELDEIIDALIVHDQAQKMESLNV

Sequences:

>Translated_359_residues
MDKIFEQLDGLLDRYEELQELMSDPEVISDTKRYLALSKEEGGMRDVVAAYKKYKQVLSDIKESEEVLRESKDDDMEALA
KEDLEDLQKQKADLEDQIKVLMLPKDPNDDKNIIMEIRGAAGGDEASLFAGDLLNMYMHYAERQGWKTEIIDATPTEVGG
YKEVVVMITGDNVYSKLKYENGAHRVQRVPVTESAGRVHTSTATVGVMPEYDEVDLKIDPKDIRTDVYRSSGAGGQHVNK
TSSAVRMTHIPSGIVVSMQDERSQQENRARAMQILRSRVYDYYETQNQEKYDQNRKNAIGTGDRSERIRTYNYPQNRVTD
HRIGLTLNKLDRIMNGELDEIIDALIVHDQAQKMESLNV
>Mature_359_residues
MDKIFEQLDGLLDRYEELQELMSDPEVISDTKRYLALSKEEGGMRDVVAAYKKYKQVLSDIKESEEVLRESKDDDMEALA
KEDLEDLQKQKADLEDQIKVLMLPKDPNDDKNIIMEIRGAAGGDEASLFAGDLLNMYMHYAERQGWKTEIIDATPTEVGG
YKEVVVMITGDNVYSKLKYENGAHRVQRVPVTESAGRVHTSTATVGVMPEYDEVDLKIDPKDIRTDVYRSSGAGGQHVNK
TSSAVRMTHIPSGIVVSMQDERSQQENRARAMQILRSRVYDYYETQNQEKYDQNRKNAIGTGDRSERIRTYNYPQNRVTD
HRIGLTLNKLDRIMNGELDEIIDALIVHDQAQKMESLNV

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=302, Percent_Identity=42.7152317880795, Blast_Score=268, Evalue=4e-72,
Organism=Homo sapiens, GI34577120, Length=349, Percent_Identity=35.5300859598854, Blast_Score=238, Evalue=7e-63,
Organism=Homo sapiens, GI166795305, Length=207, Percent_Identity=40.5797101449275, Blast_Score=184, Evalue=8e-47,
Organism=Escherichia coli, GI1787462, Length=356, Percent_Identity=48.314606741573, Blast_Score=355, Evalue=2e-99,
Organism=Escherichia coli, GI2367172, Length=339, Percent_Identity=37.1681415929204, Blast_Score=214, Evalue=8e-57,
Organism=Caenorhabditis elegans, GI17542784, Length=351, Percent_Identity=33.6182336182336, Blast_Score=189, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6321295, Length=318, Percent_Identity=42.7672955974843, Blast_Score=246, Evalue=3e-66,
Organism=Drosophila melanogaster, GI19921226, Length=335, Percent_Identity=37.910447761194, Blast_Score=218, Evalue=4e-57,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_LACC3 (Q03A29)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_806385.1
- ProteinModelPortal:   Q03A29
- SMR:   Q03A29
- STRING:   Q03A29
- GeneID:   4421612
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_1155
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- PhylomeDB:   Q03A29
- ProtClustDB:   PRK00591
- BioCyc:   LCAS321967:LSEI_1155-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40964; Mature: 40964

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKIFEQLDGLLDRYEELQELMSDPEVISDTKRYLALSKEEGGMRDVVAAYKKYKQVLSD
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHH
IKESEEVLRESKDDDMEALAKEDLEDLQKQKADLEDQIKVLMLPKDPNDDKNIIMEIRGA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEEEECC
AGGDEASLFAGDLLNMYMHYAERQGWKTEIIDATPTEVGGYKEVVVMITGDNVYSKLKYE
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEEEEECCCHHHHHCCC
NGAHRVQRVPVTESAGRVHTSTATVGVMPEYDEVDLKIDPKDIRTDVYRSSGAGGQHVNK
CCHHHEEECCCCCCCCCEEECCEEEECCCCCCCCEEEECHHHHHHHHHHCCCCCCCCCCC
TSSAVRMTHIPSGIVVSMQDERSQQENRARAMQILRSRVYDYYETQNQEKYDQNRKNAIG
HHHHEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
TGDRSERIRTYNYPQNRVTDHRIGLTLNKLDRIMNGELDEIIDALIVHDQAQKMESLNV
CCCCCCCCEECCCCCCCCCCHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDKIFEQLDGLLDRYEELQELMSDPEVISDTKRYLALSKEEGGMRDVVAAYKKYKQVLSD
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHH
IKESEEVLRESKDDDMEALAKEDLEDLQKQKADLEDQIKVLMLPKDPNDDKNIIMEIRGA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEEEECC
AGGDEASLFAGDLLNMYMHYAERQGWKTEIIDATPTEVGGYKEVVVMITGDNVYSKLKYE
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEEEEECCCHHHHHCCC
NGAHRVQRVPVTESAGRVHTSTATVGVMPEYDEVDLKIDPKDIRTDVYRSSGAGGQHVNK
CCHHHEEECCCCCCCCCEEECCEEEECCCCCCCCEEEECHHHHHHHHHHCCCCCCCCCCC
TSSAVRMTHIPSGIVVSMQDERSQQENRARAMQILRSRVYDYYETQNQEKYDQNRKNAIG
HHHHEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
TGDRSERIRTYNYPQNRVTDHRIGLTLNKLDRIMNGELDEIIDALIVHDQAQKMESLNV
CCCCCCCCEECCCCCCCCCCHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA