BacMap

Definition	Lactobacillus casei ATCC 334, complete genome.
Accession	NC_008526
Length	2,895,264

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The map label for this gene is recQ [H]

Identifier: 116494582

GI number: 116494582

Start: 1065162

End: 1066940

Strand: Direct

Name: recQ [H]

Synonym: LSEI_1081

Alternate gene names: 116494582

Gene position: 1065162-1066940 (Clockwise)

Preceding gene: 116494581

Following gene: 116494585

Centisome position: 36.79

GC content: 49.02

Gene sequence:

>1779_bases
ATGATTGCGCCGCAAGCGGAGACAATTTTAAAGGAAAAATTTGGCTATGATCATTTTCGTGCTGGTCAAAATCAGGCCAT
CAGTCGCGTTTTGGCTGGTGAAAGCACGCTGGTTGTGATGCCAACAGGCGGCGGGAAATCGTTGTGTTATCAGATTCCAG
CGATGTTGTTGTCGGGATTGACGCTTGTGGTGTCGCCGCTCATCGCGTTGATGAAGGATCAGGTGGATGCGCTGAACGAT
AACGGGATTCCGGCGGCTTTCATTAACAGCACGCTCGATTATCAGGAGATTGCTGATCGCCTTGATCAAGCAAGAAGTGG
TCAGATTAAATTATTATATGTTGCCCCAGAACGGTTTGACACGGATTGGTTTGTGCAACGGCTTGGCAGCACCGATATTT
CGTTATTTGCAATTGATGAAGCCCATTGTATTTCCCAGTGGGGGCACGATTTTCGTCCGAGTTATTTGAATCTGGCGAAT
GTGGCGGCTCAGTTGCCTTCGCAGCCACCAGTCATTGCCTTAACTGCGACGGCCACACCGCGAGTTGCGCAGGATATTTG
CCAGCGACTGGCGATTCCGGCTGACGGGATGATTAATACTGGTTTTGAGCGAAGTAATCTGAGCTTCAAGGTCGTACGCG
ATCAGGACGAAGATCGGTATCTATTAGATTACCTGAAACTGAATGTCGATCAGGCAGGCATCATCTATGCCAGCACCCGT
AAAGAAGTTGACCGTTTGTATACCTTTTTGGCACATAAGAAGTTGCCTGTGGCCAAATATCACGCGGGCATGAGTGAAGC
GCAGCGTGCTGCTAATCAGGAAGATTTTTTGTTCGATCGCAAACCAATTATGATTGCAACGAATGCGTTCGGCATGGGTA
TTGATAAAAGCAACGTGCGGTTTGTCATTCACGCCCAGATTCCCAAAGATGTCGAATCGTATTATCAAGAAGCCGGTCGG
GCTGGTCGTGATGGCTTGCCGAGTGAGGCAATTTTGCTGTTTAAGCCGCAAGATCTCCAGGTTCAGCGGTTCTTCATCGA
TCAATCCGAAGGCGATGACGAGCACAAACAGCGGCAATATGAGAAGTTGAAAATGATGGAACGGTATGCGAACACAGATC
AGTGTTTGCAACAGTTTATTTTGAACTATTTCGGTCAAAACGGGACAAAACCATGTGGTCGGTGTTCGAATTGTTTGGAT
GACCGGGAATCAGTGGATGTTACGGTTGATGCGCAGAAGGTCTTATCTTGTGTGGTTCGCTTGCATGAACGTTTTGGCAA
AGGCGTTGTGGCACAAGTGTTGGCTGGGGCCCACAATCAACGGGTATTGTCGTTTCATCTAGATCAATTGTCAACTTATG
GGCTGATGAGCAGTCGTCGTCAACGCGACATTACAACCTTCATAGATTTTCTGGCGGCTGGCGGTTATTTGGAAACTCGC
GGTGGTCAGTACCCAACTCTGGGCTTAACGGTCAAAGGCGCTGAGGTTCTTCGCGGACAAGCGCAAGTATTCCAGAAGAC
GGCGCAAAAAGCCGAGCAGCATCTCGAAGTCGACGACACTGTCTTTCAGGAACTGCGCAGTACTCGGCGACAACTGGCGG
AGCAGCAGCATTTACCGCCTTATATGATTTTCTCTGATAAGACGTTGAAGGCAATTGGCAGCGCCATGCCGCAAAGTCTT
GATGAGTTGATGGCAGTCAAAGGCGTCGGGCAGGCGAAGTTGGATAAGTATGGCCAAATCTTCTTGGATGTCCTGCAGGC
GATTAAGGCCAAGGCTTGA

Upstream 100 bases:

>100_bases
GCAAGCATTTTGTGAATGAATGTAGACAAGTGAGAGGGCACATGTGCCCGATGTCATAATCTATAAGTGAAGCCTTTTGT
TCTTTAGTAGGGGGAAGACG

Downstream 100 bases:

>100_bases
ATGGCTAGTGTCGATCAAAAAAGGTCGTGATCGCGTCTTTCCCGTTTTGAAGCGGGGGGATGCGGTCACGGCCTTTTTAG
CTTTTTACCAGGTTTTCTCC

Product: superfamily II DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 592; Mature: 592

Protein sequence:

>592_residues
MIAPQAETILKEKFGYDHFRAGQNQAISRVLAGESTLVVMPTGGGKSLCYQIPAMLLSGLTLVVSPLIALMKDQVDALND
NGIPAAFINSTLDYQEIADRLDQARSGQIKLLYVAPERFDTDWFVQRLGSTDISLFAIDEAHCISQWGHDFRPSYLNLAN
VAAQLPSQPPVIALTATATPRVAQDICQRLAIPADGMINTGFERSNLSFKVVRDQDEDRYLLDYLKLNVDQAGIIYASTR
KEVDRLYTFLAHKKLPVAKYHAGMSEAQRAANQEDFLFDRKPIMIATNAFGMGIDKSNVRFVIHAQIPKDVESYYQEAGR
AGRDGLPSEAILLFKPQDLQVQRFFIDQSEGDDEHKQRQYEKLKMMERYANTDQCLQQFILNYFGQNGTKPCGRCSNCLD
DRESVDVTVDAQKVLSCVVRLHERFGKGVVAQVLAGAHNQRVLSFHLDQLSTYGLMSSRRQRDITTFIDFLAAGGYLETR
GGQYPTLGLTVKGAEVLRGQAQVFQKTAQKAEQHLEVDDTVFQELRSTRRQLAEQQHLPPYMIFSDKTLKAIGSAMPQSL
DELMAVKGVGQAKLDKYGQIFLDVLQAIKAKA

Sequences:

>Translated_592_residues
MIAPQAETILKEKFGYDHFRAGQNQAISRVLAGESTLVVMPTGGGKSLCYQIPAMLLSGLTLVVSPLIALMKDQVDALND
NGIPAAFINSTLDYQEIADRLDQARSGQIKLLYVAPERFDTDWFVQRLGSTDISLFAIDEAHCISQWGHDFRPSYLNLAN
VAAQLPSQPPVIALTATATPRVAQDICQRLAIPADGMINTGFERSNLSFKVVRDQDEDRYLLDYLKLNVDQAGIIYASTR
KEVDRLYTFLAHKKLPVAKYHAGMSEAQRAANQEDFLFDRKPIMIATNAFGMGIDKSNVRFVIHAQIPKDVESYYQEAGR
AGRDGLPSEAILLFKPQDLQVQRFFIDQSEGDDEHKQRQYEKLKMMERYANTDQCLQQFILNYFGQNGTKPCGRCSNCLD
DRESVDVTVDAQKVLSCVVRLHERFGKGVVAQVLAGAHNQRVLSFHLDQLSTYGLMSSRRQRDITTFIDFLAAGGYLETR
GGQYPTLGLTVKGAEVLRGQAQVFQKTAQKAEQHLEVDDTVFQELRSTRRQLAEQQHLPPYMIFSDKTLKAIGSAMPQSL
DELMAVKGVGQAKLDKYGQIFLDVLQAIKAKA
>Mature_592_residues
MIAPQAETILKEKFGYDHFRAGQNQAISRVLAGESTLVVMPTGGGKSLCYQIPAMLLSGLTLVVSPLIALMKDQVDALND
NGIPAAFINSTLDYQEIADRLDQARSGQIKLLYVAPERFDTDWFVQRLGSTDISLFAIDEAHCISQWGHDFRPSYLNLAN
VAAQLPSQPPVIALTATATPRVAQDICQRLAIPADGMINTGFERSNLSFKVVRDQDEDRYLLDYLKLNVDQAGIIYASTR
KEVDRLYTFLAHKKLPVAKYHAGMSEAQRAANQEDFLFDRKPIMIATNAFGMGIDKSNVRFVIHAQIPKDVESYYQEAGR
AGRDGLPSEAILLFKPQDLQVQRFFIDQSEGDDEHKQRQYEKLKMMERYANTDQCLQQFILNYFGQNGTKPCGRCSNCLD
DRESVDVTVDAQKVLSCVVRLHERFGKGVVAQVLAGAHNQRVLSFHLDQLSTYGLMSSRRQRDITTFIDFLAAGGYLETR
GGQYPTLGLTVKGAEVLRGQAQVFQKTAQKAEQHLEVDDTVFQELRSTRRQLAEQQHLPPYMIFSDKTLKAIGSAMPQSL
DELMAVKGVGQAKLDKYGQIFLDVLQAIKAKA

Specific function: Probable DNA helicase. Required for DNA repair and intramolecular recombination; probably has overlapping function with recS (AC P50729). It probably acts to help generate ss-DNA from ds-DNA breaks [H]

COG id: COG0514

COG function: function code L; Superfamily II DNA helicase

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Localized throughout the nucleoid in the presence or absence of DNA double- strand breaks [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HRDC domain [H]

Homologues:

Organism=Homo sapiens, GI4557365, Length=631, Percent_Identity=33.5974643423138, Blast_Score=300, Evalue=3e-81,
Organism=Homo sapiens, GI169218196, Length=475, Percent_Identity=38.5263157894737, Blast_Score=272, Evalue=6e-73,
Organism=Homo sapiens, GI110735439, Length=475, Percent_Identity=38.5263157894737, Blast_Score=272, Evalue=7e-73,
Organism=Homo sapiens, GI51242947, Length=362, Percent_Identity=39.7790055248619, Blast_Score=245, Evalue=1e-64,
Organism=Homo sapiens, GI51242943, Length=362, Percent_Identity=39.7790055248619, Blast_Score=245, Evalue=1e-64,
Organism=Homo sapiens, GI148612816, Length=420, Percent_Identity=37.1428571428571, Blast_Score=245, Evalue=1e-64,
Organism=Homo sapiens, GI14591902, Length=460, Percent_Identity=32.8260869565217, Blast_Score=237, Evalue=2e-62,
Organism=Homo sapiens, GI14591904, Length=460, Percent_Identity=32.8260869565217, Blast_Score=237, Evalue=2e-62,
Organism=Homo sapiens, GI284005309, Length=350, Percent_Identity=38.8571428571429, Blast_Score=221, Evalue=2e-57,
Organism=Homo sapiens, GI262118318, Length=356, Percent_Identity=24.1573033707865, Blast_Score=77, Evalue=6e-14,
Organism=Homo sapiens, GI169881239, Length=169, Percent_Identity=30.1775147928994, Blast_Score=77, Evalue=6e-14,
Organism=Homo sapiens, GI13514809, Length=169, Percent_Identity=30.1775147928994, Blast_Score=77, Evalue=6e-14,
Organism=Homo sapiens, GI301171475, Length=169, Percent_Identity=28.9940828402367, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI301171467, Length=169, Percent_Identity=28.9940828402367, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI87196351, Length=169, Percent_Identity=28.9940828402367, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI216548263, Length=356, Percent_Identity=24.1573033707865, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI262231852, Length=356, Percent_Identity=24.1573033707865, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI21071032, Length=343, Percent_Identity=21.2827988338192, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI13518015, Length=356, Percent_Identity=24.1573033707865, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI13514831, Length=340, Percent_Identity=23.8235294117647, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI48994977, Length=592, Percent_Identity=41.2162162162162, Blast_Score=462, Evalue=1e-131,
Organism=Escherichia coli, GI87082221, Length=342, Percent_Identity=23.3918128654971, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25145561, Length=645, Percent_Identity=31.0077519379845, Blast_Score=295, Evalue=5e-80,
Organism=Caenorhabditis elegans, GI32564293, Length=467, Percent_Identity=35.7601713062098, Blast_Score=271, Evalue=7e-73,
Organism=Caenorhabditis elegans, GI71987997, Length=500, Percent_Identity=34.6, Blast_Score=266, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI71987993, Length=500, Percent_Identity=34.6, Blast_Score=265, Evalue=4e-71,
Organism=Caenorhabditis elegans, GI17552866, Length=448, Percent_Identity=33.0357142857143, Blast_Score=226, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI17507945, Length=333, Percent_Identity=23.7237237237237, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71987143, Length=364, Percent_Identity=23.0769230769231, Blast_Score=73, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71995724, Length=353, Percent_Identity=24.929178470255, Blast_Score=69, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17507121, Length=353, Percent_Identity=24.6458923512748, Blast_Score=69, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI71982858, Length=212, Percent_Identity=25, Blast_Score=67, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71995718, Length=370, Percent_Identity=25.4054054054054, Blast_Score=66, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323844, Length=475, Percent_Identity=34.3157894736842, Blast_Score=265, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6320224, Length=360, Percent_Identity=22.5, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324217, Length=346, Percent_Identity=23.9884393063584, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324778, Length=143, Percent_Identity=27.972027972028, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24646066, Length=635, Percent_Identity=31.9685039370079, Blast_Score=291, Evalue=1e-78,
Organism=Drosophila melanogaster, GI24664226, Length=424, Percent_Identity=34.6698113207547, Blast_Score=241, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24664230, Length=424, Percent_Identity=34.4339622641509, Blast_Score=240, Evalue=2e-63,
Organism=Drosophila melanogaster, GI62472181, Length=424, Percent_Identity=34.4339622641509, Blast_Score=240, Evalue=2e-63,
Organism=Drosophila melanogaster, GI21358123, Length=357, Percent_Identity=37.8151260504202, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI17977678, Length=339, Percent_Identity=22.4188790560472, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI17985987, Length=242, Percent_Identity=25.2066115702479, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004589
- InterPro:   IPR006293
- InterPro:   IPR002121
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR010997
- InterPro:   IPR018982 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C; PF00570 HRDC; PF09382 RQC [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 66162; Mature: 66162

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50967 HRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAPQAETILKEKFGYDHFRAGQNQAISRVLAGESTLVVMPTGGGKSLCYQIPAMLLSGL
CCCCHHHHHHHHHCCCHHHHCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHH
TLVVSPLIALMKDQVDALNDNGIPAAFINSTLDYQEIADRLDQARSGQIKLLYVAPERFD
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCC
TDWFVQRLGSTDISLFAIDEAHCISQWGHDFRPSYLNLANVAAQLPSQPPVIALTATATP
HHHHHHHHCCCCEEEEEECHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCC
RVAQDICQRLAIPADGMINTGFERSNLSFKVVRDQDEDRYLLDYLKLNVDQAGIIYASTR
HHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEEEHHH
KEVDRLYTFLAHKKLPVAKYHAGMSEAQRAANQEDFLFDRKPIMIATNAFGMGIDKSNVR
HHHHHHHHHHHHCCCCHHHHHCCHHHHHHHCCCCCCEECCCCEEEEECCCCCCCCCCCCE
FVIHAQIPKDVESYYQEAGRAGRDGLPSEAILLFKPQDLQVQRFFIDQSEGDDEHKQRQY
EEEEECCCHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCHHHHHHHH
EKLKMMERYANTDQCLQQFILNYFGQNGTKPCGRCSNCLDDRESVDVTVDAQKVLSCVVR
HHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEECHHHHHHHHHH
LHERFGKGVVAQVLAGAHNQRVLSFHLDQLSTYGLMSSRRQRDITTFIDFLAAGGYLETR
HHHHHCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECC
GGQYPTLGLTVKGAEVLRGQAQVFQKTAQKAEQHLEVDDTVFQELRSTRRQLAEQQHLPP
CCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
YMIFSDKTLKAIGSAMPQSLDELMAVKGVGQAKLDKYGQIFLDVLQAIKAKA
EEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIAPQAETILKEKFGYDHFRAGQNQAISRVLAGESTLVVMPTGGGKSLCYQIPAMLLSGL
CCCCHHHHHHHHHCCCHHHHCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHH
TLVVSPLIALMKDQVDALNDNGIPAAFINSTLDYQEIADRLDQARSGQIKLLYVAPERFD
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCC
TDWFVQRLGSTDISLFAIDEAHCISQWGHDFRPSYLNLANVAAQLPSQPPVIALTATATP
HHHHHHHHCCCCEEEEEECHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCC
RVAQDICQRLAIPADGMINTGFERSNLSFKVVRDQDEDRYLLDYLKLNVDQAGIIYASTR
HHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEEEHHH
KEVDRLYTFLAHKKLPVAKYHAGMSEAQRAANQEDFLFDRKPIMIATNAFGMGIDKSNVR
HHHHHHHHHHHHCCCCHHHHHCCHHHHHHHCCCCCCEECCCCEEEEECCCCCCCCCCCCE
FVIHAQIPKDVESYYQEAGRAGRDGLPSEAILLFKPQDLQVQRFFIDQSEGDDEHKQRQY
EEEEECCCHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCHHHHHHHH
EKLKMMERYANTDQCLQQFILNYFGQNGTKPCGRCSNCLDDRESVDVTVDAQKVLSCVVR
HHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEECHHHHHHHHHH
LHERFGKGVVAQVLAGAHNQRVLSFHLDQLSTYGLMSSRRQRDITTFIDFLAAGGYLETR
HHHHHCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECC
GGQYPTLGLTVKGAEVLRGQAQVFQKTAQKAEQHLEVDDTVFQELRSTRRQLAEQQHLPP
CCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
YMIFSDKTLKAIGSAMPQSLDELMAVKGVGQAKLDKYGQIFLDVLQAIKAKA
EEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 9642195 [H]