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The map label for this gene is ygcA [C]

Identifier: 116494563

GI number: 116494563

Start: 1045045

End: 1046409

Strand: Direct

Name: ygcA [C]

Synonym: LSEI_1061

Alternate gene names: 116494563

Gene position: 1045045-1046409 (Clockwise)

Preceding gene: 116494562

Following gene: 116494564

Centisome position: 36.09

GC content: 47.62

Gene sequence:

>1365_bases
TTGGCCCAACCAGCTGTAACCAAGAACCAAGATCTGGAAGTTACCATTCAAGATCTCACTTATGAAGGGATGGGCGTTGC
CAAAGTCGACGGCTTTCCGCTTTTCATTGAAGATGCCCTGCCTGGCGAAAAAATGCAGGTGCATGTCCTCAAAACGCAGA
AACAATATGGCTTTGCCAAAGTTGTCAAACGATTGAGCGACTCACCGGATCGTGTTCCCGGTGCAGATAGCACTTATATC
CGTACGGGGATTGCGCCACTGTCGATCTTGGCATATCCGGCACAATTGCAATTTAAGCAGAAACAAATTCAAGAACTTTA
TAAAAAAGCGCATCTTGATATTGCGGTGCTGCCGACCATTGGGATGACGAATCCTTTAGGCTATCGCAACAAGGCGCAAG
TACCGGTTCGTACCGTGAATGGCGAACTGCAAACTGGCTTTTATAAAAAACACAGCCACGACCTTGTACCAATTGAAGAT
TTTCTGATTCAAGATCCGAAAATTGACCAAACCATTGTGGTGGTGCGTGATCTTTTACGAAAATACCATGTGCCAGCTTA
TGATGAACGCGCTAATAAAGGCGTTATTCGTAACATCATGGTGCGCCGGGGCCATTATACGAAGCAGATGATGATTGTCT
TGGTGTCACAAACTTGGCAAGTGCCGCATCTCAAAGATATTGTCGAAGAGATCACCTTAGCATTACCAGAAGTAACGAGT
GTGGTTGTGAACTTGAATGACAAACGAACCAACGTCATTCTAGGCCGCAAGTCACGAACCATTTACGGCGAGGACTATTT
GCTGGATAAGTTGCTAGGCAGTGAATTCCAGATCTCGCCAGTCAGTTTTTATCAGGTTAATCCGATTCAGACTGAGGTGC
TTTACAAAAAGGCCATTGAGGCTGCTGGTTTGACTGGTAAGGAAAATGTGATCGATGCTTATTCAGGTATCGGGACGATT
TCCTTGACGATGGCACGTCATGCCAAACATGTTTACGGCGTCGAAGTGGTTGAACCTGCTGTGCGCGATGCACGGGCAAA
CGCGGTGCGCAATCACATTGATAATGTCACCTTTGAAGTCGGCAAGGCGGAGGAGGTCATCCAACGCTGGAAAGACAACG
AACTGCCAGTGGATGTGCTCATGGTCGATCCGCCGCGCAAAGGACTGGATAAAAGTTTCATTGACGCTGTCGGTTATATG
AAGCCGCCAAAGATCGTCTATGTGTCCTGCAACTCCGCGACTTTGGCAAGAGATTTGCAGCTGCTAGCCGCCTTTGGCTA
CACAGCAAAGGAAACCCAGCCAGTGGACATGTTCCCGCAGACGCAGCATATTGAGAGTATTACGGCGTTGACGTTGGCGA
AGTAA

Upstream 100 bases:

>100_bases
CAGAAGCAGAAGGACTACATGGCCGAAGTCGAATCTATTTCTCATCAAGATATTGATGGTGATGGACAGATCGGCTCACA
AGACGAAAAGGATGACTAAT

Downstream 100 bases:

>100_bases
GACTGCACAACTATCATGGCGCAACTCAAAGAGGCACCTGCACATCAATTGATTGCTGCGGGTGCCTCTTTTTGACTGTA
GCAGGTATGAATATCTGTTG

Product: tRNA (uracil-5-)-methyltransferase related enzyme

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MAQPAVTKNQDLEVTIQDLTYEGMGVAKVDGFPLFIEDALPGEKMQVHVLKTQKQYGFAKVVKRLSDSPDRVPGADSTYI
RTGIAPLSILAYPAQLQFKQKQIQELYKKAHLDIAVLPTIGMTNPLGYRNKAQVPVRTVNGELQTGFYKKHSHDLVPIED
FLIQDPKIDQTIVVVRDLLRKYHVPAYDERANKGVIRNIMVRRGHYTKQMMIVLVSQTWQVPHLKDIVEEITLALPEVTS
VVVNLNDKRTNVILGRKSRTIYGEDYLLDKLLGSEFQISPVSFYQVNPIQTEVLYKKAIEAAGLTGKENVIDAYSGIGTI
SLTMARHAKHVYGVEVVEPAVRDARANAVRNHIDNVTFEVGKAEEVIQRWKDNELPVDVLMVDPPRKGLDKSFIDAVGYM
KPPKIVYVSCNSATLARDLQLLAAFGYTAKETQPVDMFPQTQHIESITALTLAK

Sequences:

>Translated_454_residues
MAQPAVTKNQDLEVTIQDLTYEGMGVAKVDGFPLFIEDALPGEKMQVHVLKTQKQYGFAKVVKRLSDSPDRVPGADSTYI
RTGIAPLSILAYPAQLQFKQKQIQELYKKAHLDIAVLPTIGMTNPLGYRNKAQVPVRTVNGELQTGFYKKHSHDLVPIED
FLIQDPKIDQTIVVVRDLLRKYHVPAYDERANKGVIRNIMVRRGHYTKQMMIVLVSQTWQVPHLKDIVEEITLALPEVTS
VVVNLNDKRTNVILGRKSRTIYGEDYLLDKLLGSEFQISPVSFYQVNPIQTEVLYKKAIEAAGLTGKENVIDAYSGIGTI
SLTMARHAKHVYGVEVVEPAVRDARANAVRNHIDNVTFEVGKAEEVIQRWKDNELPVDVLMVDPPRKGLDKSFIDAVGYM
KPPKIVYVSCNSATLARDLQLLAAFGYTAKETQPVDMFPQTQHIESITALTLAK
>Mature_453_residues
AQPAVTKNQDLEVTIQDLTYEGMGVAKVDGFPLFIEDALPGEKMQVHVLKTQKQYGFAKVVKRLSDSPDRVPGADSTYIR
TGIAPLSILAYPAQLQFKQKQIQELYKKAHLDIAVLPTIGMTNPLGYRNKAQVPVRTVNGELQTGFYKKHSHDLVPIEDF
LIQDPKIDQTIVVVRDLLRKYHVPAYDERANKGVIRNIMVRRGHYTKQMMIVLVSQTWQVPHLKDIVEEITLALPEVTSV
VVNLNDKRTNVILGRKSRTIYGEDYLLDKLLGSEFQISPVSFYQVNPIQTEVLYKKAIEAAGLTGKENVIDAYSGIGTIS
LTMARHAKHVYGVEVVEPAVRDARANAVRNHIDNVTFEVGKAEEVIQRWKDNELPVDVLMVDPPRKGLDKSFIDAVGYMK
PPKIVYVSCNSATLARDLQLLAAFGYTAKETQPVDMFPQTQHIESITALTLAK

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=415, Percent_Identity=29.3975903614458, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI51173878, Length=415, Percent_Identity=29.3975903614458, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI269784680, Length=471, Percent_Identity=24.6284501061571, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI269784676, Length=471, Percent_Identity=24.6284501061571, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI20149688, Length=471, Percent_Identity=24.6284501061571, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI269784678, Length=355, Percent_Identity=27.3239436619718, Blast_Score=108, Evalue=7e-24,
Organism=Escherichia coli, GI1789148, Length=469, Percent_Identity=30.0639658848614, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1787083, Length=362, Percent_Identity=23.2044198895028, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1790403, Length=217, Percent_Identity=30.8755760368664, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6322909, Length=440, Percent_Identity=26.1363636363636, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24666579, Length=425, Percent_Identity=30.5882352941176, Blast_Score=145, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 50903; Mature: 50771

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS50926 TRAM ; PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQPAVTKNQDLEVTIQDLTYEGMGVAKVDGFPLFIEDALPGEKMQVHVLKTQKQYGFAK
CCCCCCCCCCCCEEEEEEECCCCCCEEEECCCEEEEECCCCCCCEEEEEEECCHHCCHHH
VVKRLSDSPDRVPGADSTYIRTGIAPLSILAYPAQLQFKQKQIQELYKKAHLDIAVLPTI
HHHHHCCCCCCCCCCCCCEEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEECCC
GMTNPLGYRNKAQVPVRTVNGELQTGFYKKHSHDLVPIEDFLIQDPKIDQTIVVVRDLLR
CCCCCCCCCCCCCCEEEECCCCHHCCEECCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHH
KYHVPAYDERANKGVIRNIMVRRGHYTKQMMIVLVSQTWQVPHLKDIVEEITLALPEVTS
HHCCCCCCCCCCCCHHHHHHHHCCCCHHHHHEEEECCCCCCCHHHHHHHHHHHHCCCCEE
VVVNLNDKRTNVILGRKSRTIYGEDYLLDKLLGSEFQISPVSFYQVNPIQTEVLYKKAIE
EEEECCCCCEEEEEECCCCEEECCHHHHHHHCCCCEEECCEEEEEECCCHHHHHHHHHHH
AAGLTGKENVIDAYSGIGTISLTMARHAKHVYGVEVVEPAVRDARANAVRNHIDNVTFEV
HCCCCCCCHHHHHHCCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEC
GKAEEVIQRWKDNELPVDVLMVDPPRKGLDKSFIDAVGYMKPPKIVYVSCNSATLARDLQ
CCHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCEEEEEECCCHHHHHHHH
LLAAFGYTAKETQPVDMFPQTQHIESITALTLAK
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHEECC
>Mature Secondary Structure 
AQPAVTKNQDLEVTIQDLTYEGMGVAKVDGFPLFIEDALPGEKMQVHVLKTQKQYGFAK
CCCCCCCCCCCEEEEEEECCCCCCEEEECCCEEEEECCCCCCCEEEEEEECCHHCCHHH
VVKRLSDSPDRVPGADSTYIRTGIAPLSILAYPAQLQFKQKQIQELYKKAHLDIAVLPTI
HHHHHCCCCCCCCCCCCCEEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEECCC
GMTNPLGYRNKAQVPVRTVNGELQTGFYKKHSHDLVPIEDFLIQDPKIDQTIVVVRDLLR
CCCCCCCCCCCCCCEEEECCCCHHCCEECCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHH
KYHVPAYDERANKGVIRNIMVRRGHYTKQMMIVLVSQTWQVPHLKDIVEEITLALPEVTS
HHCCCCCCCCCCCCHHHHHHHHCCCCHHHHHEEEECCCCCCCHHHHHHHHHHHHCCCCEE
VVVNLNDKRTNVILGRKSRTIYGEDYLLDKLLGSEFQISPVSFYQVNPIQTEVLYKKAIE
EEEECCCCCEEEEEECCCCEEECCHHHHHHHCCCCEEECCEEEEEECCCHHHHHHHHHHH
AAGLTGKENVIDAYSGIGTISLTMARHAKHVYGVEVVEPAVRDARANAVRNHIDNVTFEV
HCCCCCCCHHHHHHCCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEC
GKAEEVIQRWKDNELPVDVLMVDPPRKGLDKSFIDAVGYMKPPKIVYVSCNSATLARDLQ
CCHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCEEEEEECCCHHHHHHHH
LLAAFGYTAKETQPVDMFPQTQHIESITALTLAK
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566 [H]