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The map label for this gene is smpB

Identifier: 116494483

GI number: 116494483

Start: 960885

End: 961358

Strand: Direct

Name: smpB

Synonym: LSEI_0977

Alternate gene names: 116494483

Gene position: 960885-961358 (Clockwise)

Preceding gene: 116494482

Following gene: 116494485

Centisome position: 33.19

GC content: 46.84

Gene sequence:

>474_bases
ATGGCGAAGAAACATCGACAAAAACCTGACAACCTGCTCGCCCAGAATAAAAAGGCTGGTCATGATTACAACATTCTTGA
TACCTACGAAGCCGGTATTGCGCTGACAGGTACCGAAATCAAATCCGTGCGTGATGGCAAACTTAATTTACGTGACGGTT
TTGCCCGAATTCGGAATAATGAGGCGTGGTTAGAAAACGTCCACATTAGTCCGTATAAAGAGGGCAATTTATTCAACGTT
GACCCAATGCGTAATCGCAAGCTGCTCTTGCACAAACGAGAAATTCGCAAGTTAGGTCAAATGACAGCGCGCCAAGGTGT
CACGCTTGTGCCGTTGCGGATGTATCTAAAACACGGCTATGCCAAGGTTCTGATTGGTGTGGCTGAAGGGAAGCATAACT
ACGACAAACGTGAAACCTTAAAGCGCAAGGATCAGGAACGCGAGGTTCAGCGCGCTTTGAAGGCACGGTATTAA

Upstream 100 bases:

>100_bases
CGACCACGCAAACCAGCTGGTGCGCCACATCAAGGCAAGCACTATAATGCCAAGCGCGGCACTAAATAACGCTGGCAACT
TTTGAAAGGGGGATGGTTCA

Downstream 100 bases:

>100_bases
ACGCCCAGTCGCCCAGTGGCATTAAGCTAATGCTGATGTGAGCATCAAAAATCAAACTTCTAGCTCGTCCATCAGCAATA
TCATGATGTCGCTGATGTGC

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MAKKHRQKPDNLLAQNKKAGHDYNILDTYEAGIALTGTEIKSVRDGKLNLRDGFARIRNNEAWLENVHISPYKEGNLFNV
DPMRNRKLLLHKREIRKLGQMTARQGVTLVPLRMYLKHGYAKVLIGVAEGKHNYDKRETLKRKDQEREVQRALKARY

Sequences:

>Translated_157_residues
MAKKHRQKPDNLLAQNKKAGHDYNILDTYEAGIALTGTEIKSVRDGKLNLRDGFARIRNNEAWLENVHISPYKEGNLFNV
DPMRNRKLLLHKREIRKLGQMTARQGVTLVPLRMYLKHGYAKVLIGVAEGKHNYDKRETLKRKDQEREVQRALKARY
>Mature_156_residues
AKKHRQKPDNLLAQNKKAGHDYNILDTYEAGIALTGTEIKSVRDGKLNLRDGFARIRNNEAWLENVHISPYKEGNLFNVD
PMRNRKLLLHKREIRKLGQMTARQGVTLVPLRMYLKHGYAKVLIGVAEGKHNYDKRETLKRKDQEREVQRALKARY

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=156, Percent_Identity=41.6666666666667, Blast_Score=117, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_LACC3 (Q03AJ7)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_806217.1
- ProteinModelPortal:   Q03AJ7
- SMR:   Q03AJ7
- STRING:   Q03AJ7
- GeneID:   4418883
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_0977
- eggNOG:   COG0691
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- PhylomeDB:   Q03AJ7
- ProtClustDB:   PRK05422
- BioCyc:   LCAS321967:LSEI_0977-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 18302; Mature: 18171

Theoretical pI: Translated: 10.79; Mature: 10.79

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKHRQKPDNLLAQNKKAGHDYNILDTYEAGIALTGTEIKSVRDGKLNLRDGFARIRNN
CCCCCCCCCHHHHHCCCCCCCCCCCEECCCCCEEEECHHHHHCCCCCCCHHHHHHHHCCC
EAWLENVHISPYKEGNLFNVDPMRNRKLLLHKREIRKLGQMTARQGVTLVPLRMYLKHGY
HHHHHCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHCCH
AKVLIGVAEGKHNYDKRETLKRKDQEREVQRALKARY
HHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKKHRQKPDNLLAQNKKAGHDYNILDTYEAGIALTGTEIKSVRDGKLNLRDGFARIRNN
CCCCCCCCHHHHHCCCCCCCCCCCEECCCCCEEEECHHHHHCCCCCCCHHHHHHHHCCC
EAWLENVHISPYKEGNLFNVDPMRNRKLLLHKREIRKLGQMTARQGVTLVPLRMYLKHGY
HHHHHCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHCCH
AKVLIGVAEGKHNYDKRETLKRKDQEREVQRALKARY
HHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA